Abstract：
:While decades of research have elucidated many steps of the alphavirus lifecycle, the earliest replication dynamics have remained unclear. This missing time window has obscured early replicase strand-synthesis behavior and prevented elucidation of how the first events of infection might influence subsequent viral competition. Using quantitative live-cell and single-molecule imaging, we observed the initial replicase activity and its strand preferences in situ and measured the trajectory of replication over time. Under this quantitative framework, we investigated viral competition, where one alphavirus is able to exclude superinfection by a second homologous virus. We show that this appears as an indirect phenotypic consequence of a bidirectional competition between the two species, coupled with the rapid onset of viral replication and a limited total cellular carrying capacity. Together, these results emphasize the utility of analyzing viral kinetics within single cells.
journal_name
Cell Systjournal_title
Cell systemsauthors
Singer ZS,Ambrose PM,Danino T,Rice CMdoi
10.1016/j.cels.2020.12.005subject
Has Abstractpub_date
2021-01-21 00:00:00eissn
2405-4712issn
2405-4720pii
S2405-4712(20)30506-8pub_type
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