Abstract：
:Spodoptera littoralis is a phytophagous generalist. Its host range includes more than 40 plant species, some of which produce 3-nitropropanoic acid (3-NPA), an irreversible inhibitor of mitochondrial succinate dehydrogenase. Growth in larvae fed an artificial diet with a sublethal admixture of 3-NPA (4.2 μmol per g) was slowed significantly, but larvae experienced no increase in mortality. In contrast, larvae injected with 25.2 μmol/g (bodyweight) 3-NPA experienced acute toxicity and death. To study the detoxification mechanism of 3-NPA in S. littoralis, the insect frass was analyzed by HPLC-MS. Comparative analysis of 3-NPA-treated and -untreated control samples using HR-MS(2) revealed a group of differential signals that were identified as amino acid amides of 3-NPA with glycine, alanine, serine, and threonine. When sublethal amounts of stable isotope-labeled 3-NPA were injected into a larva's hemolymph, 3-NPA amino acid conjugates were identified as putative detoxification products. Bioassays with synthetic standards confirmed that the toxicity of the amides was negligible in comparison to the toxicity of free 3-NPA, demonstrating that amino acid conjugation in S. littoralis represents an efficient way to detoxify 3-NPA. Furthermore, biosynthetic studies using crude fractions of the gut tissue indicated that conjugation of 3-NPA with amino acids occurs in epithelial cells of the insect's gut. Taken together, these results suggest that the detoxification of 3-NPA in S. littoralis proceeds via conjugation to specific amino acids within the epithelial cells followed by export of the nontoxic amino acid conjugates to the hemolymph via as yet uncharacterized mechanisms, most likely involving the Malpighian tubules.
journal_name
Insect Biochem Mol Bioljournal_title
Insect biochemistry and molecular biologyauthors
Novoselov A,Becker T,Pauls G,von Reuß SH,Boland Wdoi
10.1016/j.ibmb.2015.05.013subject
Has Abstractpub_date
2015-08-01 00:00:00pages
97-103eissn
0965-1748issn
1879-0240pii
S0965-1748(15)00103-4journal_volume
63pub_type
杂志文章abstract：:Resistance to insecticides by modification of their molecular targets is a serious problem in chemical control of many arthropod pests. Neonicotinoids target the nicotinic acetylcholine receptor (nAChR) of arthropods. The spectrum of possible resistance-conferring mutations of this receptor is poorly understood. Predi...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2007.12.007
更新日期：2008-05-01 00:00:00
abstract：:Molecules in the midgut of the tsetse fly (Diptera: Glossinidiae) are thought to play an important role in the life cycle of African trypanosomes by influencing their initial establishment in the midgut and subsequent differentiation events that ultimately affect parasite transmission. It is thus important to determin...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(02)00063-2
更新日期：2002-11-01 00:00:00
abstract：:Levels of mRNA for the two 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthases, (HMG-S1 and HMG-S2), and for HMG-CoA reductase (HMG-R) of Blattella germanica were analyzed in the fat body during the first gonadotrophic cycle. HMG-S2 and HMG-R showed the highest mRNA levels on day 0 and decreased thereafter, wher...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(96)00044-6
更新日期：1996-09-01 00:00:00
abstract：:Mosquito salivary proteins, which are fundamental to the process of blood feeding, also facilitate disease transmission and cause allergic reactions. The identification and characterisation of these proteins have been hampered by the difficulty of obtaining them in purified form. In this report, we describe the produc...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(99)00066-1
更新日期：1999-10-01 00:00:00
abstract：:Orcokinin (NFDEIDRSGFGFN) and orcokinin homologues are crustacean peptides eliciting potent myotropic effects in gut tissues. Through HPLC purification of brain extract of the cockroach Blattella germanica, we isolated the first insect orcokinin (NFDEIDRSGFNS). This insect orcokinin-like peptide do not show myotropic ...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2004.07.005
更新日期：2004-11-01 00:00:00
abstract：:In this report, we describe the glutathione transferase (GST) gene family in the dengue vector Aedes aegypti and suggest a novel role for a new class of mosquito GSTs. Twenty-six GST genes are present in Ae. aegypti, two of which are alternatively spliced to give a total of 29 transcripts for cytosolic GSTs. The six c...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2007.05.018
更新日期：2007-10-01 00:00:00
abstract：:Methylfarnesoate (MF), an analogue of the insect juvenile hormone III, has been implicated to play a vital role in the regulation of the growth and reproductive development in crustaceans. Farnesoic acid O-methyltransferase (FAMeT) is the key enzyme involved in catalyzing the final step in the MF biosynthetic pathway....
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(01)00060-1
更新日期：2001-10-01 00:00:00
abstract：:The correlation between the natural levels of GST and the tolerance to the insecticide decamethrin (dMT), as well as the interaction between the molecules of affinity purified enzyme and the insecticide were investigated in order to collect further information on the obscure role of the Glutathione S-transferase syste...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(00)00123-5
更新日期：2001-03-15 00:00:00
abstract：:A number of insect ecdysteroids, plant ecdysteroids and juvenoids were assayed for their ability to activate Drosophila nuclear receptors in transfected tissue culture cells. Discrete modifications to 20-hydroxyecdysone, the apparent natural ligand for the ecdysone receptor (EcR), conferred dramatic changes on the tra...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(00)00075-8
更新日期：2000-11-01 00:00:00
abstract：:The importance of Chagas disease motivated the scientific effort to obtain the complete genomic sequence of the vector species Rhodnius prolixus, this information is also relevant to the understanding of triatomine biology in general. The central nervous system is the key regulator of insect physiology and behavior. N...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2015.05.003
更新日期：2016-02-01 00:00:00
abstract：:The phytophagous stink bug, Nezara viridula (L.) infests multiple plant species and impacts agricultural production worldwide. We analyzed the transcriptomes of N. viridula accessory salivary gland (ASG), principal salivary gland (PSG) and gut, with a focus on putative digestive proteases and nucleases that present a ...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2018.10.003
更新日期：2018-12-01 00:00:00
abstract：:A novel serine protease, named as scolonase, was purified and characterized from the tissue of the Korean centipede, Scolopendra subspinipes mutilans. Purified scolonase showed an apparent molecular weight of 25 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and an isoelectric point of 4.8 o...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2003.10.003
更新日期：2004-03-01 00:00:00
abstract：:Uridine diphosphate (UDP)-glycosyltransferases (UGTs) catalyze the addition of UDP-sugars to small hydrophobic molecules, turning them into more water-soluble metabolites. While their role in detoxification is well documented for vertebrates, arthropod UGTs have only recently been linked to the detoxification and sequ...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2019.04.010
更新日期：2019-06-01 00:00:00
abstract：:A blood meal induces the ovaries of female Aedes aegypti mosquitoes to produce ecdysteroid hormones that regulate many processes required for egg maturation. Various proteins involved in the intracellular transport and biosynthesis of ecdysteroid precursors have been identified by analysis of Drosophila melanogaster m...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2005.01.011
更新日期：2005-05-01 00:00:00
abstract：:Most moths utilize sex pheromones released by the female to attract a mate. Females produce the sex pheromone in the pheromone gland in a biosynthetic pathway which consists of several key enzymes. Fatty acyl-CoA reductase is one of the key enzymes, which catalyzes the conversion of fatty acyl-CoA to the corresponding...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2019.103260
更新日期：2020-01-01 00:00:00
abstract：:When the biotinylated Manduca sexta adipokinetic hormone gene was used as a probe for in situ hybridization, the intrinsic neurosecretory cells were stained with a biotin detection system that contained streptavidin or avidin. Further experiments showed that the DNA probe was not necessary for staining these cells by ...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/0965-1748(94)00095-y
更新日期：1995-05-01 00:00:00
abstract：:There is increasing interest in the development of effective mosquito repellents of natural origin to reduce transmission of diseases such as malaria and yellow fever. To achieve this we have employed an in vitro competition assay involving odorant-binding proteins (OBPs) of the malaria mosquito, Anopheles gambiae, wi...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2018.03.008
更新日期：2018-05-01 00:00:00
abstract：:Approximately 30-40% of the dry weight of an insect egg consists of lipid, mostly triacylglycerol (TAG). Although this lipid is essential for the energy needed by the developing embryo, little is known about the mechanism that leads to the accumulation of TAG in the insect egg. Insect oocytes can readily synthesize TA...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章,评审
doi：10.1016/j.ibmb.2006.01.014
更新日期：2006-04-01 00:00:00
abstract：:The ecdysone-inducible E75 gene responsible for the 75B puff of Drosophila melanogaster encodes a family of proteins which are members of the steroid receptor superfamily. These proteins are believed to be involved in the regulation of ecdysone response. In order to investigate the evolutionary conservation of E75, we...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/0965-1748(93)90086-8
更新日期：1993-01-01 00:00:00
abstract：:We have previously cloned and characterized BmJHE, a juvenile hormone (JH)-selective esterase (JHE) that is important for JH titer regulation in the silkworm Bombyx mori. Here, we sought to determine whether multiple genes might function as JH-specific esterase in this species. We searched for putative carboxyl/cholin...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2009.12.015
更新日期：2010-02-01 00:00:00
abstract：:Wood feeding insects depends heavily on the secretion of a combination of cellulases, mainly endoglucanases and other glucanases such as exoglucanases and xylanases, for efficient digestion of the cellulosic materials. To date, although a high number of endoglucanases have been found in xytophagous insects, little is ...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2012.05.002
更新日期：2012-09-01 00:00:00
abstract：:Larvae of the Chrysomelina species Phaedon cochleariae and Gastrophysa viridula produce monoterpenoids (iridoids) to defend themselves against predatory attacks by presenting the toxins upon attack as droplets on the top of nine pairs of dorsal glands. Although the conversion of 8-hydroxygeraniol-8-O-beta-d-glucoside ...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2006.11.011
更新日期：2007-03-01 00:00:00
abstract：:In addition to its wide role in metabolism, iron in insects has been implicated in vitellogenesis and the immune response. The NRAMP family comprises a well-conserved family of divalent cation transporters in metazoans. To gain insight on the role of NRAMP in Anopheles albimanus, we cloned a cDNA encoding a 571-residu...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2007.02.009
更新日期：2007-06-01 00:00:00
abstract：:Ribonucleotide reductase catalyses the de novo synthesis of deoxyribonucleotides. Class I reductases use an iron center to generate a tyrosyl free radical that can initiate formation of the deoxyribonucleotide. These reductases are alpha 2 beta 2 holoenzymes, and the subunits are denoted as R1 and R2. R1 contains the ...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(02)00041-3
更新日期：2002-09-01 00:00:00
abstract：:Phenoloxidase (PO), a melanin-synthesizing enzyme known to play an important role in insect defense, is found as a zymogen (ProPO) in hemolymph and cuticle, where it is activated by proteolysis. We characterized the first proPO cDNA in an eusocial insect, the Apis mellifera honey bee. The AmproPO cDNA contains an ORF ...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2005.01.013
更新日期：2005-06-01 00:00:00
abstract：:Juvenile hormone esterase (JHE, EC 3.1.1.1) from whole Drosophila melanogaster prepupae has previously been purified by selective precipitations, isoelectric focussing and two column chromatography steps. JHE bands from dried silver-stained SDS-PAGE gels of that material were digested with trypsin. The masses of the t...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(01)00035-2
更新日期：2001-04-27 00:00:00
abstract：:In an effort to study the mode of action of Cry11Ba, we identified toxin binding proteins in Anopheles gambiae larval midgut and investigated their receptor roles. Previously, an aminopeptidase (AgAPN2) and an alkaline phosphatase (AgALP1) were identified as receptors for Cry11Ba toxin in A. gambiae. However, an A. ga...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2012.11.007
更新日期：2013-02-01 00:00:00
abstract：:In this work, we have explored the biochemical changes characterizing the transition from vitellogenesis to follicular atresia, employing the hematophagous insect vector Dipetalogaster maxima as a model. Standardized insect rearing conditions were established to induce a gradual follicular degeneration stage by depriv...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2011.06.005
更新日期：2011-10-01 00:00:00
abstract：:The male ejaculate contains a multitude of seminal fluid proteins (SFPs), many of which are key reproductive molecules, as well as sperm. However, the identification of SFPs is notoriously difficult and a detailed understanding of this complex phenotype has only been achieved in a few model species. We employed a rece...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2018.12.002
更新日期：2019-01-01 00:00:00
abstract：:Among disease-vectors, the evolution of the tick innate immune system is still lagging when compared to insects. Such an investigation, which was initiated, by first cloning and sequencing lectins associated in the innate immunity of invertebrates and having fibrinogen related domains, helped in the sequencing of cDNA...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2005.04.001
更新日期：2005-09-01 00:00:00
