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Ribonucleotide reductase subunits from the yellow fever mosquito, Aedes aegypti: cloning and expression.

Abstract：

:Ribonucleotide reductase catalyses the de novo synthesis of deoxyribonucleotides. Class I reductases use an iron center to generate a tyrosyl free radical that can initiate formation of the deoxyribonucleotide. These reductases are alpha 2 beta 2 holoenzymes, and the subunits are denoted as R1 and R2. R1 contains the allosteric binding site and the active site, whereas R2 contains a binuclear iron center that initiates formation of the tyrosyl radical. We have cloned and sequenced the cDNAs encoding the R1 and R2 subunit in the yellow fever mosquito, Aedes aegypti. The messages for these proteins are increased in response to blood-feeding.

journal_name

Insect Biochem Mol Biol

journal_title

Insect biochemistry and molecular biology

authors

Pham DQ,Blachuta BJ,Nichol H,Winzerling JJ

doi

10.1016/s0965-1748(02)00041-3

subject

Has Abstract

pub_date

2002-09-01 00:00:00

pages

1037-44

issue

9

eissn

0965-1748

issn

1879-0240

pii

S0965174802000413

journal_volume

32

pub_type

杂志文章

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