Synthesis of hydrolysis-resistant pyridoxal 5'-phosphate analogs and their biochemical and X-ray crystallographic characterization with the pyridoxal phosphatase chronophin.

Abstract:

:A set of phosphonic acid derivatives (1-4) of pyridoxal 5'-phosphate (PLP) was synthesized and characterized biochemically using purified murine pyridoxal phosphatase (PDXP), also known as chronophin. The most promising compound 1 displayed primarily competitive PDXP inhibitory activity with an IC50 value of 79μM, which was in the range of the Km of the physiological substrate PLP. We also report the X-ray crystal structure of PDXP bound to compound 3, which we solved to 2.75Å resolution (PDB code 5AES). The co-crystal structure proves that compound 3 binds in the same orientation as PLP, and confirms the mode of inhibition to be competitive. Thus, we identify compound 1 as a PDXP phosphatase inhibitor. Our results suggest a strategy to design new, potent and selective PDXP inhibitors, which may be useful to increase the sensitivity of tumor cells to treatment with cytotoxic agents.

journal_name

Bioorg Med Chem

authors

Knobloch G,Jabari N,Stadlbauer S,Schindelin H,Köhn M,Gohla A

doi

10.1016/j.bmc.2015.02.049

subject

Has Abstract

pub_date

2015-06-15 00:00:00

pages

2819-27

issue

12

eissn

0968-0896

issn

1464-3391

pii

S0968-0896(15)00153-4

journal_volume

23

pub_type

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