Interaction between nitrous oxide and diazepam in the mouse staircase test.

Abstract:

:The interaction between nitrous oxide and diazepam was assessed in the mouse staircase test. In this paradigm, the numbers of rears (NR) (simultaneous standing on hindlegs and sniffing the air) and steps ascended (NSA) reflect anxiety and locomotor activity, respectively. Antianxiety drugs characteristically reduce NR but not NSA, while sedative drugs reduce both NR and NSA in generally parallel fashion. In this study, increasing concentrations of nitrous oxide alone produced progressively greater and statistically significant increases in NSA. Nitrous oxide alone had no significant effect on NR until, at a concentration of 75%, it decreased NR. Diazepam alone decreased NR equally and significantly at all doses. With certain combinations of concentration and dose, nitrous oxide enhanced the effect of diazepam on NR without altering NSA. These findings suggest that nitrous oxide can increase the antianxiety effect of diazepam without increasing its sedative effect in the mouse staircase paradigm.

journal_name

Anesth Analg

journal_title

Anesthesia and analgesia

authors

Pruhs RJ,Quock RM

subject

Has Abstract

pub_date

1989-04-01 00:00:00

pages

501-5

issue

4

eissn

0003-2999

issn

1526-7598

journal_volume

68

pub_type

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