Abstract:
:To determine whether epidermal growth factor (EGF) or EGF-like transforming growth factors (TGFs) are present in ovine fetal tissues, we have tested acid-ethanol extracts of ovine fetal kidney for the ability to induce anchorage-independent growth of normal rat kidney fibroblasts in soft agar and to compete with 125I-mouse EGF for binding to receptors in sheep liver. The fetal kidney extract (20-1000 micrograms protein/ml) stimulated a dose-dependent increase in the number of soft agar colonies of normal rat kidney cells. Approximately 70% of these colonies measured greater than or equal to 3100 microM2. The fetal kidney extract was dissolved in 1 M acetic acid and chromatographed on Bio-Gel P 10. Two peaks of TGF-like activity, with approximate MW 14.5K (peak 1) and 9K (peak 2), eluted from the column. Half-maximal effects of pooled peaks 1 and 2 on colony formation were achieved using 100 and 20 micrograms protein/ml, respectively. Peaks 1 and 2 also competed with 125I-mouse EGF for binding to EGF receptors in ovine fetal liver but had no activity in a homologous mouse EGF radioimmunoassay sensitive to 15 pg mouse EGF. Neither TGF activity nor EGF receptor binding activity was detected in Bio-Gel fractions co-eluting with 125I-mouse EGF. Specific 125I-EGF binding sites in fetal liver were detected as early as midgestation, and the number of EGF binding sites increased markedly in late gestation, exceeding the number of EGF binding sites in the livers of pregnant ewes. These findings demonstrate the presence of TGF-like activity in ovine fetal kidney and high affinity EGF receptors in ovine fetal liver.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Pediatr Resjournal_title
Pediatric researchauthors
Freemark M,Comer Mdoi
10.1203/00006450-198711000-00026subject
Has Abstractpub_date
1987-11-01 00:00:00pages
609-15issue
5eissn
0031-3998issn
1530-0447journal_volume
22pub_type
杂志文章abstract::Small for gestational age (SGA) children have a higher prevalence of cardiovascular risk factors at a young age. It is not known whether this increased risk is caused by their size at birth, a familial predisposition for cardiovascular disease or smallness at birth or a combination of these factors. The cardiovascular...
journal_title:Pediatric research
pub_type: 杂志文章,多中心研究
doi:10.1203/PDR.0b013e3181732922
更新日期:2008-07-01 00:00:00
abstract::The intranasal live attenuated influenza vaccine (LAIV), FluMist, has been widely appreciated by pediatricians, parents, and children alike for its ease of administration. However, concerns regarding lack of effectiveness in recent influenza seasons led to the CDC Advisory Committee on Immunization Practices (ACIP) re...
journal_title:Pediatric research
pub_type: 杂志文章,评审
doi:10.1038/pr.2017.239
更新日期:2018-01-01 00:00:00
abstract::Children treated for acute lymphoblastic leukemia may develop reduced bone mineral density during treatment, but there is little information on the mechanisms involved. In a prospective, longitudinal study on 15 children with ALL, we undertook serial measurements of markers of bone and collagen turnover, insulin-like ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-200010000-00012
更新日期:2000-10-01 00:00:00
abstract::A double-label, double-substrate incubation technique has been developed and used to study the conversion of progesterone to testosterone in testes extracts from incompletely virilized males. The procedure involves separation of the microsomes from a testicular homogenate, incubating the microsomes with 1 microM [7-3H...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198408000-00018
更新日期:1984-08-01 00:00:00
abstract::Kinetic data are presented for the decarboxylation of branched chain alpha-ketoacids (BCKA) by intact human fibroblasts. Cultured cells of normal individuals and nine patients with different clinical pictures of maple syrup urine disease (MSUD) are studied with both alpha-ketoisocaproic acid (2-oxo-4-methylpentanoic a...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-197509000-00005
更新日期:1975-09-01 00:00:00
abstract::Perinatal brain injury is a leading cause of death and disability in young children. Recent advances in obstetrics, reproductive medicine and neonatal intensive care have resulted in significantly higher survival rates of preterm or sick born neonates, at the price of increased prevalence of neurological, behavioural ...
journal_title:Pediatric research
pub_type: 杂志文章,评审
doi:10.1038/s41390-018-0222-6
更新日期:2019-01-01 00:00:00
abstract::The present study examined the extent to which abundance of the prolactin receptor (PRLR) and a range of primary mitochondrial proteins are influenced by either maternal nutrition and/or fetal number in adipose tissue. Pregnant sheep were control fed [consuming 100% of total metabolizable energy (ME) requirements (tak...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000047653.73271.C4
更新日期:2003-02-01 00:00:00
abstract::Alterations in the pancreatic secretion of fluid and of enzymes in response to either pilocarpine (15 mg/kg) or an octapeptide of cholecystokinin (0.1 microgram/kg) have been found in rats that received daily injections of reserpine (0.5 mg/kg) for 7 days. During a 3-hr secretory period, significant reductions in the ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198109000-00015
更新日期:1981-09-01 00:00:00
abstract::We measured the changes in tidal volume, duration of the various phases of the respiratory cycle, and peak nasal pressure during elastic and resistive loading in preterm infants. Values were calculated during the first loaded breath, when chemical drive was unchanged. Tidal volume decreased by equivalent percentages w...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-197704000-00002
更新日期:1977-04-01 00:00:00
abstract::We have previously demonstrated that vagal phase-response curves (PRC), which characterize the effects of critically timed, brief vagal stimuli on sinus node automaticity, exhibit a fundamentally different shape in the canine newborn than in the adult. In this study we analyzed the changes in sinus cycle length in res...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199401000-00013
更新日期:1994-01-01 00:00:00
abstract::Epithelial hyperplasia is an early feature of the renal and biliary lesions in autosomal recessive polycystic kidney disease (ARPKD). To explore the cellular basis of this hyperplasia we isolated, cultured, and characterized biliary tract epithelium from common bile duct explants of mice with ARPKD (the BPK mouse) and...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199506000-00014
更新日期:1995-06-01 00:00:00
abstract::Previous studies have shown that early life stress in the form of intermittent maternal separation (MS) predisposes adult rats to develop stress-induced intestinal mucosal dysfunction and visceral hypersensitivity. However, the mechanism involved in the functional abnormalities is unclear. Our aim was to study immatur...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.pdr.0000190577.62426.45
更新日期:2006-01-01 00:00:00
abstract::Naloxone has been shown to reverse the hemodynamic sequelae of experimental septic shock in adult animal models. Its effectiveness in the newborn has not been studied. To further investigate the efficacy of naloxone, we instrumented 18 piglets for continuous measurement of mean arterial pressure, mean pulmonary arteri...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198608000-00001
更新日期:1986-08-01 00:00:00
abstract::Alterations of the architecture of cerebral white matter in the developing human brain can affect cortical development and result in functional disabilities. A line scan diffusion-weighted magnetic resonance imaging (MRI) sequence with diffusion tensor analysis was applied to measure the apparent diffusion coefficient...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199810000-00019
更新日期:1998-10-01 00:00:00
abstract::A method is developed for the detection of homozygotes and heterozygote carriers of Niemann-Pick disease type A by employing a high performance liquid chromatography (HPLC) technique for the measurement of sphingomyelin (SP) as well as for the assay of the enzyme sphingomyelinase (SPase) in skin fibroblasts and amniot...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-197805000-00008
更新日期:1978-05-01 00:00:00
abstract::In view of their known high incidence of noninsulin dependent diabetes (NIDDM), we sought to determine whether Native American (Plains Indian) children and adolescents show evidence of risk factors for both NIDDM and cardiovascular disease. Children and adolescents between the ages of 4 and 19 y were recruited, and fi...
journal_title:Pediatric research
pub_type: 临床试验,杂志文章
doi:10.1203/00006450-199607000-00014
更新日期:1996-07-01 00:00:00
abstract:BACKGROUND:Postnatal growth restriction (PNGR) in premature infants increases risk of pulmonary hypertension (PH). In a rodent model, PNGR causes PH, while combining PNGR and hyperoxia increases PH severity. We hypothesized that PNGR causes intestinal dysbiosis and that treatment with a probiotic attenuates PNGR-associ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/s41390-019-0578-2
更新日期:2020-02-01 00:00:00
abstract::Previous studies have shown that proton magnetic resonance spectroscopy (MRS) is useful in predicting neurologic prognosis in children with traumatic brain injury (TBI). Reductions in N-acetyl derived metabolites and presence of lactate have been predictive of poor outcomes. We examined another spectroscopy metabolite...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000139928.60530.7D
更新日期:2004-10-01 00:00:00
abstract::Cerebral magnetic resonance imaging (MRI) has revealed white matter changes in patients with phenylketonuria (PKU), an inborn error of metabolism with increased plasma phenylalanine level. Because the significance of these lesions is unknown, this study was undertaken to determine whether glucose metabolism was depres...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199607000-00004
更新日期:1996-07-01 00:00:00
abstract::We examined adult sheep lymphocytes for the following surface markers: Surface membrane immunoglobulin, antigen identified by anti-sheep thymocyte globulin and complement receptors. We quantitated sheep peripheral blood mononuclear leukocyte proliferative responses to mitogens and alloantigens, and demonstrated a neut...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198405000-00012
更新日期:1984-05-01 00:00:00
abstract:INTRODUCTION:The Coulter LH780 hematology analyzer can evaluate mean neutrophil volume (MNV), conductivity (MNC), scatter (MNS), and distribution width (DW). We sought to investigate the value of volume, conductivity, and scatter (VCS) parameters in diagnosis and treatment efficacy of neonatal sepsis. RESULTS:We obser...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2011.16
更新日期:2012-01-01 00:00:00
abstract::There is great interest in the regenerative potential of the neural stem cells and progenitors that populate the germinal zones of the immature brain. Studies using animal models of pediatric brain injuries have provided a clearer understanding of the responses of these progenitors to injury. In this review, we have c...
journal_title:Pediatric research
pub_type: 杂志文章,评审
doi:10.1038/pr.2017.261
更新日期:2018-01-01 00:00:00
abstract::Heme oxygenase (HO), the rate-limiting enzyme in the formation of bilirubin, is expressed in the lung and may serve as an antioxidant. This enzyme results in the formation of antioxidant bile pigments and the degradation of pro-oxidant heme. We wanted to evaluate the differences in expression of HO-1, the inducible fo...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-200301000-00010
更新日期:2003-01-01 00:00:00
abstract::Gitelman disease was diagnosed in two unrelated children with hypokalemic metabolic alkalosis and growth failure (a boy and a girl aged 7 mo and 9.5 y, respectively, at clinical presentation) on the basis of mutations detected in the gene encoding the thiazide-sensitive NaCl cotransporter of the distal convoluted tubu...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199908000-00017
更新日期:1999-08-01 00:00:00
abstract::The objective of this study was to investigate changes of cerebral oxygenation and hemodynamics related to opening of the bypass bridge during veno-arterial extracorporeal membrane oxygenation (ECMO). Ten newborn infants and 12 piglets were studied during opening of the bridge for 10 and 1 s, respectively. Changes in ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199507000-00022
更新日期:1995-07-01 00:00:00
abstract::Spleens from 1-20-wk-old guinea pigs infected in utero with Treponema pallidum and age-matched controls, born to normal and heat-killed (56 degrees C, 2 h.) T. pallidum-injected mothers, were examined for their in vitro lymphoproliferative response to phytohemagglutinin, concanavalin A, and lipopolysaccharide. Additio...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199712000-00013
更新日期:1997-12-01 00:00:00
abstract:BACKGROUND:Hurricane Maria struck Puerto Rico on 20 September 2017 causing catastrophic devastation. Prolonged shortage of food had been a substantial challenge to the residents after Maria. Experiencing food insecurity in utero has been associated with negative health outcomes later in life. We aim to examine whether ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/s41390-020-0839-0
更新日期:2020-12-01 00:00:00
abstract:BACKGROUND:Maternal smoking impairs fetal growth; however, if postnatal growth differs between children born small for gestational age (SGA) with smoking and non-smoking mother is unknown. METHODS:Cohort-study of term born children born appropriate for gestational age with non-smoking mother (AGA-NS, n = 30,561), SGA ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/s41390-019-0352-5
更新日期:2019-06-01 00:00:00
abstract::To investigate the detailed pattern of change in circulating gonadotropin and testosterone concentrations around the onset of puberty and to determine the temporal relationship between the gonadotropin and testosterone secretion, plasma gonadotropin and testosterone were measured at 20-min intervals for 24 h in 21 nor...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199308000-00026
更新日期:1993-08-01 00:00:00
abstract::There is in vitro evidence that the ductus venosus of the fetal and newborn lamb relaxes to administration of E-series prostaglandins. We performed this study to determine whether the ductus venosus of the newborn lamb could be reopened by an infusion of prostaglandin E1. Fifteen lambs between 4 and 28 h of life and s...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198703000-00002
更新日期:1987-03-01 00:00:00