Abstract:
:Alterations in the pancreatic secretion of fluid and of enzymes in response to either pilocarpine (15 mg/kg) or an octapeptide of cholecystokinin (0.1 microgram/kg) have been found in rats that received daily injections of reserpine (0.5 mg/kg) for 7 days. During a 3-hr secretory period, significant reductions in the volume of pancreatic juice and in the total output of protein, amylase, and trypsin were observed in these animals. In the first hour of the secretory response, however, protease output was increased in the treated animals, particularly that of chymotrypsin, which was also increased in the longer secretory period following pilocarpine, but not cholecystokinin, stimulation. Zymogen granules isolated from the pancreas of the treated rats by differential centrifugation in a 0.3 M sucrose buffer had increased specific activities of the proteases when compared to those of untreated controls. Ultrastructurally, zymogen granules isolated from the pancreas of the treated rats showed changes in density, with bizonal and trizonal configurations being frequently observed, and had less distinct limiting membranes. In some, the membrane appeared broken at intervals, and there was granular material, presumably derived from the granule contents, lining the surface of the granule. It is concluded that pretreatment with reserpine inhibits fluid secretion and alters enzyme secretion in the rat exocrine pancreas. The latter effect is related to a nonparallel storage of amylase and proteases in the secretory granules induced by the drug treatment, probably through an action on protein synthesis or intracellular transport. An accumulation of proteases may lead to activation of these enzymes and to granule lysis. Inasmuch as the reserpine-treated rat has been proposed as an experimental model for cystic fibrosis, these findings are relevant in terms of possibly pathogenetic mechanisms in this disease.
journal_name
Pediatr Resjournal_title
Pediatric researchauthors
McCurdy RE,Martinez Rdoi
10.1203/00006450-198109000-00015subject
Has Abstractpub_date
1981-09-01 00:00:00pages
1308-13issue
9eissn
0031-3998issn
1530-0447journal_volume
15pub_type
杂志文章abstract::Normal hemostasis depends in part on the balance achieved between proaggregatory and prothrombotic platelet thromboxane A2, measured as its stable end-product thromboxane B2 (TXB2), and vascular prostacyclin (PGI2), which inhibits platelet aggregation and is antithrombotic. Cystathionine-beta-synthase deficiency is ch...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198206000-00018
更新日期:1982-06-01 00:00:00
abstract::We measured the cortisol and corticosterone responses to insulin-induced hypoglycemia in 13 growth hormone (GH)-deficient children and 30 short children without GH deficiency. Although there was no difference between the two groups in degree of hypoglycemia attained, baseline cortisol, baseline corticosterone, or cort...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198508000-00009
更新日期:1985-08-01 00:00:00
abstract::Many trials of mesenchymal stem cells (MSCs) have been published in the past 5-6 y. MSCs inhibit T-cell alloreactivity in vitro by soluble factors and direct cell-to-cell contact. They are safe to infuse in humans with no acute toxicity and no ectopic tissue formation. Promising results of MSC infusion for graft-vs.-h...
journal_title:Pediatric research
pub_type: 杂志文章,评审
doi:10.1038/pr.2011.56
更新日期:2012-04-01 00:00:00
abstract::Synchronized atrial contraction may be much more important in the newborn, who has a faster heart rate and a less compliant ventricle, than in the adult. We therefore investigated the extent by which synchronized atrioventricular contraction contributes to cardiac output and cardiac work in a neonatal circulation, and...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199803000-00004
更新日期:1998-03-01 00:00:00
abstract::There has been recent interest in the use of the phosphodiesterase-5 inhibitor sildenafil for treating pulmonary hypertension. We examined the interaction between inhaled nitric oxide (iNO) and i.v. sildenafil in 12 piglets with acute pulmonary hypertension and lung injury secondary to meconium aspiration. Six animals...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000112033.81970.C2
更新日期:2004-03-01 00:00:00
abstract::Mother's milk represents a foundational step in the proper development of newborn immunity. This is achieved, in part, through the action of numerous regulatory proteins such as soluble cluster of differentiation 14 (sCD14) found in significant quantities in human milk (~25-50 μg/mL). In adults, CD14 stimulates cytoki...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3181f70f21
更新日期:2010-12-01 00:00:00
abstract:BACKGROUND:To minimize secondary lung injury, ventilated preterm infants are extubated as soon as possible. To maximize extubation success, they are often placed in prone position. The effect of extubation and subsequent prone positioning on lung volumes is currently unknown. METHODS:Changes in end-expiratory lung vol...
journal_title:Pediatric research
pub_type: 临床试验,杂志文章
doi:10.1038/pr.2014.201
更新日期:2015-03-01 00:00:00
abstract::We examined adult sheep lymphocytes for the following surface markers: Surface membrane immunoglobulin, antigen identified by anti-sheep thymocyte globulin and complement receptors. We quantitated sheep peripheral blood mononuclear leukocyte proliferative responses to mitogens and alloantigens, and demonstrated a neut...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198405000-00012
更新日期:1984-05-01 00:00:00
abstract:BACKGROUND:Using a large population-based sample, this study aims to verify whether televiewing at 29 mo, a common early childhood pastime, is prospectively associated with school readiness at 65 mo. METHODS:Participants are a prospective longitudinal cohort of 991 girls and 1,006 boys from the Quebec Longitudinal Stu...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2013.105
更新日期:2013-09-01 00:00:00
abstract::Cancer stem cells (CSC) are recently proposed to be the cancer initiating cells responsible for tumorigenesis and contribute to cancer resistance. Advances have been made in identifying and enriching CSC in leukemia and several solid tumors, including breast, brain and lung cancers. These studies suggest that, like no...
journal_title:Pediatric research
pub_type: 杂志文章,评审
doi:10.1203/01.pdr.0000203592.04530.06
更新日期:2006-04-01 00:00:00
abstract::Labeled choline incorporation into adult rat lung alveolar epithelial cells and adult rat lung fibroblasts in monolayer culture was determined after incubation with insulin (Ins) 10 micrograms/ml, Dexamethasone (Dex) 10(-6)M, or no drug (ND). Incubation periods were 1, 3, 4, and 5 hours. The lecithin (phosphatidyl cho...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198007000-00014
更新日期:1980-07-01 00:00:00
abstract::A model of patent ductus arteriosus in premature lambs was created to examine the lamb's ability to handle the volume load imposed by a patent ductus arteriosus and to determine the lamb's ability to handle any additional volume load. Fifteen preterm lambs [133 +/- 2 (+/- SD) days gestation, term 145 days], whose duct...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198711000-00012
更新日期:1987-11-01 00:00:00
abstract::Severe tetrahydrobiopterin (BH4) deficiency is a naturally occurring model of cerebral catecholamine and serotonin shortage. Examination of the stimulated release and physiologic secretion pattern of several hormones in affected individuals permits certain conclusions concerning the involvement of these neurotransmitt...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199804000-00006
更新日期:1998-04-01 00:00:00
abstract::Neonatal hypoxic-ischemic encephalopathy (HIE) is a clinically defined neurological condition after lack of oxygen and often associated with cardiac dysfunction in term infants. Therapeutic hypothermia (HT) after birth is neuroprotective in infants with HIE. However, it is not known whether HT is also cardioprotective...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e31822941ee
更新日期:2011-10-01 00:00:00
abstract:BACKGROUND:Gender is increasingly recognized as an important influence on brain development, disease susceptibility, and response to pharmacologic/rehabilitative treatments. In regenerative medicine, it remains entirely unknown whether there is an interaction between transplanted stem cells and host gender that might b...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2014.7
更新日期:2014-05-01 00:00:00
abstract::Pregnane steroids have sedative and neuroprotective effects on the brain, due to interactions with the steroid-binding site of the GABAA receptor. In the adult brain, synthesis of the pregnane steroids is increased in response to stress. Therefore, we have used umbilicoplacental embolization to mimic chronic placental...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000088066.47755.36
更新日期:2003-12-01 00:00:00
abstract::We investigated the relative contribution of the two pathways of phosphatidylcholine (PC) synthesis in lungs from chick embryos of 12 to 20 days of incubation as well as from hatched chicks and adult chickens. Lung "blocks" were incubated for 60 min in vitro in the presence of [3H]methionine and [14C]choline. PC was s...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198105000-00008
更新日期:1981-05-01 00:00:00
abstract::Serial plasma concentrations of immunoreactive 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha), the stable hydration product of prostacyclin (PGI2), were measured with radioimmunoassay during the first month of life in 25 preterm infants with idiopathic respiratory distress syndrome (IRDS) and 38 preterm controls wi...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198210000-00005
更新日期:1982-10-01 00:00:00
abstract::Recent data suggest that umbilical venous perfusion of the fetal liver has an important influence on fetal growth and postnatal liver function, and that maternal factors in late pregnancy modify this circulation. In a longitudinal study of 160 low-risk pregnancies, we determined how umbilical and portal venous blood f...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/pdr.0b013e318163a1de
更新日期:2008-03-01 00:00:00
abstract::BackgroundAdults born preterm have higher levels of cardiometabolic risk factors than their term-born peers. Studies have suggested that at least those born smallest eat less healthily. We examined the association between early (<34 weeks) and late (34-36 weeks) preterm birth and diet and food preferences in adult age...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2017.301
更新日期:2018-03-01 00:00:00
abstract::Human milk was fractionated to obtain a partially purified growth factor preparation. The growth factor in this fraction, designated as human milk growth factor III, exhibits chromatographic and biological characteristics similar to epidermal growth factor-urogastrone. Pretreatment of mice with human milk growth facto...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198509000-00009
更新日期:1985-09-01 00:00:00
abstract::Tracheal epithelial cells obtained from adult and infant ferrets were grown in primary culture in vitro. Cells from adult animals grew readily, and many ciliated cells were observed in the cultures. Successful cultures were derived from infant animals, but cell number in infant and adult cultures began to decrease aft...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199107000-00016
更新日期:1991-07-01 00:00:00
abstract::An imbalance in apoptosis or survival of immune cells plays an essential role in the pathophysiology of sepsis. Phagocytosis-induced cell death (PICD) is a common result of the pathogen-host cell interaction mediated by reactive oxygen species (ROS). Neonatal sepsis is frequently characterized by hyperinflammation. Co...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e31815b8e9f
更新日期:2008-01-01 00:00:00
abstract::D-myo-Inositol 1,4,5-triphosphate (IP3) is a key second messenger in many cells, including macrophages, T and B cells, and neutrophils, in which it regulates free intracellular calcium ion levels. In human polymorphonuclear leukocytes the rise of intracellular [Ca2+] is the signal that activates a number of functions ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199510000-00015
更新日期:1995-10-01 00:00:00
abstract::Sudden infant death syndrome is the most common cause of postneonatal infant mortality in the developed world. It is a diagnosis of exclusion with peak age of incidence between 2 and 6 mo. Fifty to 63% of these infants have a preexisting upper respiratory tract infection before death. We hypothesized that the immature...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-200210000-00005
更新日期:2002-10-01 00:00:00
abstract::This study investigated the effects of intraventricular/periventricular blood on cerebral cAMP production and cortical cerebrovascular reactivity. Under halothane and N2O anesthesia, 3 mL of either autologous blood or artificial cerebrospinal fluid (CSF) were injected into the left caudate nucleus; volume was adequate...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199709000-00010
更新日期:1997-09-01 00:00:00
abstract::We have previously shown that milk sensitization aggravates intestinal dysfunction in the malnourished guinea pigs, suggesting that it may also impair the recovery from malnutrition. To test this hypothesis, the growing guinea pigs were malnourished by feeding only maize for 7 d and then were refed for 21 d with a bal...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199812000-00018
更新日期:1998-12-01 00:00:00
abstract::Prenatal maternal therapy with glucocorticoid reduces the incidence of respiratory distress syndrome (RDS) in premature infants. To investigate the effects of this treatment on the fetal endocrine system, we determined serum concentrations of betamethasone, cortisol, dehydroepiandrosterone sulfate, growth hormone, and...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198002000-00011
更新日期:1980-02-01 00:00:00
abstract:BACKGROUND:Irisin is a myokine secreted by skeletal muscle during physical activity. Irisin treatment increased cortical bone mineral density (BMD) in young healthy mice and restored bone and muscle mass loss in a mouse model of disuse-induced osteoporosis and muscular atrophy. In humans, Irisin was positively correlat...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/s41390-019-0278-y
更新日期:2019-03-01 00:00:00
abstract::Congenital Central Hypoventilation Syndrome (CCHS) patients show partial retention of peripheral chemoreception despite impaired ventilatory responses to CO2 and hypoxia. The condition allows examination of central responses to hyperoxia, which minimizes afferent traffic from peripheral chemoreceptors. We used functio...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000155763.93819.46
更新日期:2005-04-01 00:00:00