Abstract：
:We have recently described the isolation and purification to homogeneity of a new sialoglycopeptide from bovine brain cell surfaces that reversibly inhibits protein synthesis and DNA synthesis of normal but not transformed cells. Active inhibitory preparations, however, were shown to contain a protease activity that was not lost upon purification. Several experiments were performed to establish the relationship between the proteolytic activity of the sialoglycopeptide and the biological inhibitory activity. Both the protease activity and inhibitory activity were stable at pH 6-8 but were reduced or completely destroyed below pH 4 and above pH 9. Acid inactivation was reversible and upon dialysis, both the biological inhibitory and protease activities were regained. Deglycosylation and CNBr cleavage indicated that the polypeptide backbone, rather than carbohydrate moiety, played an important role in the protease and biological inhibitory activities. Furthermore, chemical modification of amino and tyrosine groups indicated that both residues are essential for both activities. Thus, the biological inhibitory activity and protease activity are very closely related and most likely reside with the same polypeptide sequence.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Sharifi BG,Bascom CC,Fattaey H,Nash S,Johnson TCdoi
10.1002/jcb.240310106subject
Has Abstractpub_date
1986-01-01 00:00:00pages
41-57issue
1eissn
0730-2312issn
1097-4644journal_volume
31pub_type
杂志文章abstract：:The lysosomal cysteine protease cathepsin B is implicated in degradation of extracellular matrix (ECM), a crucial step in a variety of physiological and pathological processes, including tumor dissemination and angiogenesis. In this study, we analyzed the contribution of extracellular and intracellular cathepsin B act...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
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更新日期：2006-04-15 00:00:00
abstract：:Patients with cervical cancer have abnormal cell proliferation and invasion after many years of latency. However, the precise mechanisms remain unclear. Mitogen- and stress-activated kinase 2 (MSK2) is a serine/threonine kinase which displays a phenotype that promotes tumor growth and metastasis in many different type...
journal_title：Journal of cellular biochemistry
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更新日期：2019-02-12 00:00:00
abstract：:Induced pluripotent stem cells (iPSC) hold tremendous potential for personalized cell-based repair strategies to treat musculoskeletal disorders. To establish human iPSCs as a potential source of viable chondroprogenitors for articular cartilage repair, we assessed the in vitro chondrogenic potential of the pluripoten...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.24388
更新日期：2013-02-01 00:00:00
abstract：:Gold nanoparticles (AuNP's) facilitate cancer cell recognition and can be manufactured by green synthesis using nutrient rich medicinal plants such as Moringa oleifera (MO). Targeting dysregulated oncogenes and tumor suppressor genes is crucial for cancer therapeutics. We investigated the antiproliferative effects of ...
journal_title：Journal of cellular biochemistry
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doi：10.1002/jcb.25528
更新日期：2016-10-01 00:00:00
abstract：:Annexin II is a multifunctional calcium-dependent phospholipid binding protein whose presence in epidermis has previously been reported. However, like other members of annexin family, annexin II has been regarded as either an intracellular protein or associated with the cellular membrane. Here, we report the presence ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.10256
更新日期：2002-01-01 00:00:00
abstract：:The regulatory machinery that governs genetic and epigenetic control of gene expression is compartmentalized in nuclear microenvironments. Temporal and spatial parameters of regulatory complex organization and assembly are functionally linked to biological control and are compromised with the onset and progression of ...
journal_title：Journal of cellular biochemistry
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更新日期：2008-08-15 00:00:00
abstract：:Amyloid-β protein (Aβ) is the main component of senile plaques in the brains of Alzheimer disease (AD) patients. Aβ is proteolytically derived from amyloid-β precursor protein by β- and γ-secretases. Secreted Aβ is then eliminated from the central nervous system by multiple clearance mechanisms, including phagocytosis...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
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更新日期：2017-12-01 00:00:00
abstract：:Chromium(VI) resistant Chinese hamster ovary (CHO) cell lines were established in this study by exposing parental CHO-K1 cells to sequential increases in CrO3 concentration. The final concentration of CrO3 used for selection was 7 microM for Cr7 and 16 microM for Cr16 cells. Cr16-1 was a subclone derived from Cr16 cel...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.240570410
更新日期：1995-04-01 00:00:00
abstract：:Recent data suggest that megakaryocytes (MKs) play a role in skeletal homeostasis. In vitro and in vivo data show that MKs stimulate osteoblast (OB) proliferation and inhibit osteoclast (OC) formation, thus favoring net bone deposition. There are several mouse models with dysregulated megakaryopoiesis and resultant hi...
journal_title：Journal of cellular biochemistry
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更新日期：2010-03-01 00:00:00
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journal_title：Journal of cellular biochemistry
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更新日期：2010-02-01 00:00:00
abstract：:The epigenetics and molecular biology of human embryonic stem cells (hES cells) have received much more attention than their architecture. We present a more complete look at hES cells by electron microscopy, with a special emphasis on the architecture of the nucleus. We propose that there is an ultrastructural signatu...
journal_title：Journal of cellular biochemistry
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更新日期：2017-04-01 00:00:00
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journal_title：Journal of cellular biochemistry
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journal_title：Journal of cellular biochemistry
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journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.20177
更新日期：2004-09-01 00:00:00
abstract：:Ischemic stroke is the leading cause of disabilities worldwide. MicroRNA-377 (miR-377) plays important roles in ischemic injury. The present study focused on the mechanisms of miR-377 in protecting ischemic brain injury in rats. Cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) in rats. Primary ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.26181
更新日期：2018-01-01 00:00:00
abstract：:Signal transducer and activator of transcription 1 (Stat1) is a critical mediator of gene transcription in type I interferon (IFN-alpha/beta) signaling that is essential for host defense against viruses. In the skeletal system, type I IFNs (IFN-alpha/beta) also play an important physiological role in the inhibition of...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.20316
更新日期：2005-02-01 00:00:00
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journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,随机对照试验
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更新日期：2018-11-01 00:00:00
abstract：:E-peptide of the pro-Insulin-like growth factor-I (pro-IGF-I) is produced from pre-pro-IGF-I by proteolytic cleavage in the post-translational processing. Previous in vitro studies conducted in our laboratory showed that Ea4-peptide of rainbow trout (rt) pro-IGF-I or Eb-peptide of human (h) pro-IGF-I exhibited activit...
journal_title：Journal of cellular biochemistry
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doi：10.1002/jcb.21254
更新日期：2007-08-01 00:00:00
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journal_title：Journal of cellular biochemistry
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journal_title：Journal of cellular biochemistry
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更新日期：2012-06-01 00:00:00
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journal_title：Journal of cellular biochemistry
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journal_title：Journal of cellular biochemistry
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更新日期：1982-01-01 00:00:00
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journal_title：Journal of cellular biochemistry
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更新日期：2019-11-01 00:00:00
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journal_title：Journal of cellular biochemistry
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abstract：:Pulmonary fibrosis is a lethal inflammatory disease. In this study, we aimed to explore the potential-related circular RNAs (circRNAs) and genes that are associated with pulmonary fibrosis. Pulmonary fibrosis rat models were constructed and the fibrosis deposition was detected using hematoxylin and eosin and Masson st...
journal_title：Journal of cellular biochemistry
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更新日期：2019-02-14 00:00:00
abstract：:Inflammation, elicited in the skin following tissue damage or pathogen invasion, may become chronic with deleterious consequences. Tumor necrosis factor (TNF) is a key mediator of cutaneous inflammation and the keratinocyte an important protagonist of skin immunity. Calcitriol, the hormonally active vitamin D metaboli...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
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abstract：:Monoclonal antibodies that bind a large molecular weight plasma membrane protein of Acanthamoeba castellanii cause the cells to differentiate. A different monoclonal antibody that binds specifically to the major plasma membrane protein has no effect upon cell division or differentiation. The induction of differentiati...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.240290410
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abstract：:Phosphatidylethanolamine-binding protein 4 (PEBP4), a member of the PEBP family, has been reported to play a pivotal role in tumor progression. However, its role in epithelial-to-mesenchymal transition (EMT) in non-small cell lung cancer (NSCLC) cells remains unclear. Here, we investigated the effects and underlying m...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
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更新日期：2019-04-01 00:00:00
abstract：:Fluorescent proteins have enabled the color-coding of cells growing in vivo. Noninvasive imaging of cells expressing fluorescent proteins has allowed the real-time determination of the behavior of cancer cells, the progression of infection, the differentiation of stem cells, and interaction of stromal and cancer cells...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
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更新日期：2017-09-01 00:00:00
