Abstract：
:Induced pluripotent stem cells (iPSC) hold tremendous potential for personalized cell-based repair strategies to treat musculoskeletal disorders. To establish human iPSCs as a potential source of viable chondroprogenitors for articular cartilage repair, we assessed the in vitro chondrogenic potential of the pluripotent population versus an iPSC-derived mesenchymal-like progenitor population. We found the direct plating of undifferentiated iPSCs into high-density micromass cultures in the presence of BMP-2 promoted chondrogenic differentiation, however these conditions resulted in a mixed population of cells resembling the phenotype of articular cartilage, transient cartilage, and fibrocartilage. The progenitor cells derived from human iPSCs exhibited immunophenotypic features of mesenchymal stem cells (MSCs) and developed along multiple mesenchymal lineages, including osteoblasts, adipocytes, and chondrocytes in vitro. The data indicate the derivation of a mesenchymal stem cell population from human iPSCs is necessary to limit culture heterogeneity as well as chondrocyte maturation in the differentiated progeny. Moreover, as compared to pellet culture differentiation, BMP-2 treatment of iPSC-derived MSC-like (iPSC-MSC) micromass cultures resulted in a phenotype more typical of articular chondrocytes, characterized by the enrichment of cartilage-specific type II collagen (Col2a1), decreased expression of type I collagen (Col1a1) as well as lack of chondrocyte hypertrophy. These studies represent a first step toward identifying the most suitable iPSC progeny for developing cell-based approaches to repair joint cartilage damage.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Guzzo RM,Gibson J,Xu RH,Lee FY,Drissi Hdoi
10.1002/jcb.24388subject
Has Abstractpub_date
2013-02-01 00:00:00pages
480-90issue
2eissn
0730-2312issn
1097-4644journal_volume
114pub_type
杂志文章abstract：OBJECTIVES:This study is aimed to explore the functions of long noncoding RNA (lncRNA) HEIH on the proliferation as well as metastasis of non-small cell lung cancer (NSCLC). METHODS:HEIH was shown to be increased in NSCLC by a microarray study on lncRNA profiling. We detected the expression of HEIH in different human ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.27629
更新日期：2019-03-01 00:00:00
abstract：:We report here the identification and characterization of a novel SR-related protein, referred to as SRrp37, based on its apparent molecular weight and subcellular location. SRrp37 was identified through a yeast two-hybrid screen during the course of searching for proteins interacting with pNO40, a ribosomal 60S core ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.22255
更新日期：2009-09-01 00:00:00
abstract：:In adipocytes, amino acids stimulate the target of rapamycin (TOR) signaling pathway leading to phosphorylation of the translational repressor, eIF-4E binding protein-I (4E-BP1), and ribosomal protein S6. L-leucine is the primary mediator of these effects. The structure-activity relationships of a putative L-leucine r...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/(sici)1097-4644(20000501)77:2<234::aid-jcb
更新日期：2000-03-01 00:00:00
abstract：:Arctigenin is a dibenzylbutyrolactone lignan isolated from Bardanae fructus, Arctium lappa L, Saussureamedusa, Torreya nucifera, and Ipomea cairica. It has been reported to exhibit anti-inflammatory activities, which is mainly mediated through its inhibitory effect on nuclear transcription factor-kappaB (NF-κB). But t...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
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更新日期：2011-10-01 00:00:00
abstract：:In the present study, we have investigated dexamethasone and insulin regulation of the expression of adipose-specific mRNA, namely, glycerophosphate dehydrogenase (G3PDH) and adipsin, at different stages of differentiation. During adipose conversion, insulin promotes an accumulation of G3PDH mRNA which is linked to ce...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.240420407
更新日期：1990-04-01 00:00:00
abstract：:Apoptosis has been described extensively in tooth development, which is under tight control of multiple apoptosis regulators, including anti-apoptotic protein Bcl-2. However, it is totally unclear how Bcl-2 is related to odontogenesis, especially dentinogenesis. Using a transgenic mouse Col2.3Bcl-2 in which human Bcl-...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.22722
更新日期：2010-10-01 00:00:00
abstract：:Using computer stress-induced duplex destabilization (SIDD) analysis and binding experiments, we identified a S/MAR element (-599/-200 bp) (Hp-S/MAR) adjacent to the cis-element (-165/-56 bp) in the rat haptoglobin gene. We examined its functional interactions with the lamins and lamin-associated proteins in the basal...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.22225
更新日期：2009-08-15 00:00:00
abstract：:Phospholipase D (PLD) and ADP-ribosylation factor 6 (ARF6) have been implicated in vesicular trafficking and rearrangement of the actin cytoskeleton. We have explored the co-localization of rat PLD1b and rat PLD2 with wild type and mutant forms of ARF6 in HeLa cells and studied their activation by ARF6 and the role of...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
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更新日期：2005-05-01 00:00:00
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journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28614
更新日期：2019-08-01 00:00:00
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journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.240590103
更新日期：1995-09-01 00:00:00
abstract：:Extracts of Malus hupehensis (Pamp.) Rehder, containing flavonoids with good antioxidant and antiliver injury properties, possess various biological activities. The aim of this study was to explore the antioxidant activity of these extracts in vitro and in vivo. The antioxidant activity of the extracts was studied usi...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28469
更新日期：2019-02-19 00:00:00
abstract：:Muscular dystrophies are characterized by continuous cycles of degeneration and regeneration that result in extensive fibrosis and a progressive diminution of muscle mass. Cell surface heparan sulfate proteoglycans are found almost ubiquitously on the surface and in the extracellular matrix (ECM) of mammalian cells. T...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.10184
更新日期：2002-01-01 00:00:00
abstract：:Several gap junction connexins have been shown to be essential for appropriate placental development and function. It is known that the expression and distribution of connexins change in response to environmental oxygen levels. The placenta develops under various oxygen levels, beginning at a low oxygen tension of app...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.25240
更新日期：2015-12-01 00:00:00
abstract：:Recently, glioma stem cells have been identified as the main cause of glioma propagation and recurrence and a number of several cell markers have been indicated as putative GSC markers. In the present work, a retrospective study to evaluate the prognostic potential of ability to generate GSCs in our series of 15 gliob...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.25043
更新日期：2015-05-01 00:00:00
abstract：:Rad51-mediated homologous recombination (HR) is essential for maintenance of genome integrity. The Xrcc3 protein functions in HR DNA repair, and studies suggest it has multiple roles at different stages in this pathway. Defects in vertebrate XRCC3 result in elevated levels of spontaneous and DNA damage-induced chromos...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.20232
更新日期：2004-10-15 00:00:00
abstract：:Follicle-stimulating hormone-follicle-stimulating hormone receptor (FSH-FSHR) interaction is one of the most thoroughly studied signaling pathways primarily because of being implicated in sexual reproduction in mammals by way of maintaining gonadal function and sexual fertility. Despite material advances in understand...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28044
更新日期：2018-11-02 00:00:00
abstract：:The expression of matrix metalloproteinase-13 (MMP-13), involved in bone turnover, is elevated in stretched MC3T3-E1 osteoblast-like cells. Strain-mediated forces impact bone remodeling due in large part to the movement of fluid through the canalicular-lacunar network. The resulting fluid shear stress (FSS) over the s...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21349
更新日期：2007-12-01 00:00:00
abstract：:Our previous study demonstrate that vitamin D3 induces the binding of vitamin D3 receptor (VDR) to Sp1 transcription factor and stimulates p27Kip1 expression via the Sp1 consensus sequences in the promoter. Both VDR and Sp1 are transcriptional activators, it is unclear which protein functions as the transcription comp...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.20780
更新日期：2006-08-15 00:00:00
abstract：:Macromolecules are sulfated during the vegetative growth of Dictyostelium discoideum. A characterisation of the structures of sulfated oligosaccharides associated with these macromolecules indicates that the oligosaccharides are heterogeneous. Endoglycosidase and pronase digestion were used with gel-filtration chromat...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.240380202
更新日期：1988-10-01 00:00:00
abstract：OBJECTIVE:To investigate the correlation between serum protein level of insulin growth factor 1 (IGF-1) and the degree of liver fibrosis in patients with chronic hepatitis C (CHC) combined with type 2 diabetes mellitus (T2DM). METHODS:The cases are divided into four groups. Then serum levels of IFG-1, alanine aminotra...
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pub_type： 杂志文章
doi：10.1002/jcb.27267
更新日期：2018-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1002/jcb.29124
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journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21894
更新日期：2008-10-15 00:00:00
abstract：INTRODUCTION:Pancreatic adenocarcinoma (PAAD) is one of the most fatal cancers in the world for early metastasis, extensive invasion, and poor prognosis with a 5-year survival rate less than 5%. However, the underlying mechanisms are poorly understood. Therefore, it is urgent to explore molecular markers for early diag...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28055
更新日期：2018-11-28 00:00:00
abstract：:To investigate changes in chromatin organization associated with DNA replication during the first stages of development of the sea urchin Tetrapygus niger, we compared micrococcal nuclease (MNase) digestion patterns of chromatin from zygotes harvested during the first S phase and from unfertilized eggs. We observed th...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.240590205
更新日期：1995-10-01 00:00:00
abstract：OBJECTIVE:Current study focused on the influence of miR-200b-3p on cardiocyte apoptosis of diabetic cardiomyopathy (DCM) by regulating CD36 and peroxisome proliferator-activated receptor γ (PPAR-γ) signaling pathway. METHODS:Bioinformatic analysis was used to analyze differentially expressed microRNA (miRNAs), messeng...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.27795
更新日期：2019-04-01 00:00:00
abstract：:Optimization of an in vitro culture that supports blastocyst (BL) development from single blastomeres (SBs) is essential to generate additional embryos for farm animals and humans and unravel the mechanisms that underlie totipotency. In this study, we have examined BL development from SBs that were derived from 2-cell...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.27106
更新日期：2018-09-01 00:00:00
abstract：:Recent literature provides us with the indication that the ratio of OPG/RANKL concentrations fluctuate in a non-linear (bell-shaped) mode, according to Frost's "mechanostat" theory, during the application of mechanical strain. A similar cellular signal transduction mechanism to mechanical loading, that may also regula...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.25793
更新日期：2017-05-01 00:00:00
abstract：:The cdk5/p35 complex has been implicated in a variety of functions related to brain development, including axonal outgrown and neuronal migration. In this study, by co-immunoprecipitation and pull-down experiments, we have shown that the cdk5/p35 complex associates with and phosphorylates the neuronal delta-catenin. I...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21041
更新日期：2007-02-15 00:00:00
abstract：:Membrane receptors for steroid hormones affect signaling pathways that modulate nuclear function, influence neuronal activity, ion flow, and the circulatory system. Indeed, 'new' steroid hormones have been identified by their interaction with membrane-initiated signaling systems. A brief summary of the FASEB Summer Re...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.10409
更新日期：2003-02-15 00:00:00
abstract：BACKGROUND:A critical feature for fibroblasts differentiation into myofibroblasts is the expression of alpha-smooth muscle actin (α-SMA) during the tissue injury and repair process. The epigenetic mechanism, DNA methylation, is involved in regulating α-SMA expression. It is not clear how methyl-CpG-binding protein 2 (M...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.29655
更新日期：2020-07-01 00:00:00
