Seroimmunology of AIDS retrovirus infection. I. Use of immunofluorescence assay to confirm sera with ELISA reactivity.

Abstract:

:One thousand sera shown to be reactive by one of two commercial enzyme linked immunosorbent assays (ELISA) for antibodies to the AIDS virus were referred to the NSW State Reference Laboratory for confirmatory assays. Each serum was retested by two commercial ELISA systems (Abbott and ENI), the ENI exclusionary H9 ELISA and an immunofluorescence assay. Three hundred and twenty four sera were reactive by all 3 tests whereas 244 demonstrated concordant non-reactivity. Three hundred and seventy seven sera were reactive by Abbott EIA only and could not be confirmed positive by the ENI ELISA incorporating exclusionary testing, immunofluorescence or Western immunoblot of representative sera. Sera obtained from teaching hospital laboratories were more likely to be positive and less likely to be negative by all 3 tests, and were also less likely to be Abbott EIA reactive only compared with sera obtained from the blood transfusion service. Of the remaining 55 sera, 52 demonstrated a negative immunofluorescent reaction or a pattern of equal fluorescence on AIDS virus infected and control cells. Representative sera were shown to be negative on Western immunoblot analysis. Of the 3 sera which demonstrated immunofluorescence reactivity, one was positive and one was negative by Western immunoblot, and one could not be determined. We conclude that a combination of two ELISAs, one with an exclusionary ELISA test and an immunofluorescence assay is a reliable and simple means of confirming reactive sera for AIDS virus antibodies.

journal_name

Pathology

journal_title

Pathology

authors

Imrie AA,Kehrer S,Smith GW,Penny R,Cooper DA

doi

10.3109/00313028609087565

subject

Has Abstract

pub_date

1986-10-01 00:00:00

pages

438-43

issue

4

eissn

0031-3025

issn

1465-3931

journal_volume

18

pub_type

杂志文章
  • Rhabdoid tumour: a malignancy of early childhood with variable primary site, histology and clinical behaviour.

    abstract:AIMS:To correlate the immunostaining for INI1 protein and mutations in INI1 gene in possible rhabdoid tumours (RT) and atypical teratoid/rhabdoid tumours (AT/RT) seen at the Royal Children's Hospital in the last 10 years, and to study the clinicopathological features of those patients with negative nuclear staining. M...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.1080/00313020802436451

    authors: Wu X,Dagar V,Algar E,Muscat A,Bandopadhayay P,Ashley D,Wo Chow C

    更新日期:2008-12-01 00:00:00

  • Erythroblastosis fetalis--the discovery and partial elimination of rhesus incompatibility--the origins of exchange transfusion in Australia.

    abstract::The first 2 exchange transfusions in Australia for Rhesus-induced erythroblastosis (hemolytic disease of the newborn) were conducted in the 9 mth period from December 1945 to August 1946. These pioneering endeavours in medical research were undertaken by 3 transfusionists who were, or had been, directors of the Red Cr...

    journal_title:Pathology

    pub_type: 历史文章,杂志文章

    doi:10.1080/00313029400169431

    authors: Pearn JH

    更新日期:1994-04-01 00:00:00

  • Inflammatory pseudotumor of epididymis and Epstein-Barr virus: a study of two cases.

    abstract::Inflammatory pseudotumor (IP) is an uncommon tumor-like lesion of unknown etiology. Two cases of IP of the epididymis were identified in our hospital file. Having read a recent report on finding Epstein-Barr virus (EBV) in some cases of IP in lymph node, spleen and liver, the two cases of epididymal IP were tested for...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.1080/00313029700169664

    authors: Chan KW,Chan KL,Lam KY

    更新日期:1997-02-01 00:00:00

  • Diffuse mesothelioma of the peritoneum: correlation between histological and clinical parameters and survival in 73 patients.

    abstract::There are few studies addressing survival of diffuse peritoneal mesotheliomas (DPM).In this study, survival data were obtained retrospectively from 73 patients treated with intended cytoreductive surgery for DPM, with a mean follow-up of 42 months. Mesotheliomas were classified as well differentiated papillary (WDPM, ...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.1097/PAT.0000000000000181

    authors: Liu S,Staats P,Lee M,Alexander HR,Burke AP

    更新日期:2014-12-01 00:00:00

  • The histological and ultrastructural features of the epiphyseal plate in Morquio type A syndrome (mucopolysaccharidosis type IVA).

    abstract::Tissue from the epiphyseal plate of a patient with Morquio type A syndrome (mucopolysaccharidosis type IVA) was studied by undecalcified histological and electron microscopical techniques. Cartilage cells in the plate were vacuolated to a variable degree. The orderly proliferating structure of the epiphyseal plate was...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.3109/00313028609059462

    authors: McClure J,Smith PS,Sorby-Adams G,Hopwood J

    更新日期:1986-04-01 00:00:00

  • Not only 'Flinders Island' spotted fever.

    abstract:AIM:To demonstrate that Flinders Island spotted fever (FISF), a spotted fever group rickettsial infection caused by Rickettsia honei, is found not only on Flinders Island (Bass Strait), Tasmania, but elsewhere in south-east Australia. METHODS:Cases of FISF were identified by rickettsial serology, culture and the detec...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.1080/00313020500099247

    authors: Unsworth NB,Stenos J,McGregor AR,Dyer JR,Graves SR

    更新日期:2005-06-01 00:00:00

  • Role of the renal sympathetic nervous system in mediating renal ischaemic injury-induced reductions in renal haemodynamic and excretory functions.

    abstract:AIM:We investigated the role of renal sympathetic innervation in the deterioration of renal haemodynamic and excretory functions during the early post-ischaemic phase of renal ischaemia/reperfusion injury. METHODS:Anaesthetised male Sprague-Dawley rats were subjected to unilateral renal ischaemia by clamping the left ...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.3109/00313021003631304

    authors: Salman IM,Ameer OZ,Sattar MA,Abdullah NA,Yam MF,Najim HS,Khan AH,Johns EJ

    更新日期:2010-04-01 00:00:00

  • Rare (new) entities of the breast and medullary carcinoma.

    abstract::Rare tumours are a heterogeneous group of conditions that encompass benign to malignant lesions both of stromal and epithelial origin. Here some of these tumours are described, in which the authors have most familiarity. An outline of medullary carcinoma is also presented. ...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.1080/00313020802563528

    authors: Foschini MP,Eusebi V

    更新日期:2009-01-01 00:00:00

  • Human papillomavirus update with a particular focus on cervical disease.

    abstract::Human papillomavirus (HPV) is a common viral infection of squamous epithelial tissues, but its importance has only recently been recognised by the medical community. HPVs are now realised to consist of many genotypes and are associated with a diverse spectrum of clinical manifestations. Within the genital tract, some ...

    journal_title:Pathology

    pub_type: 杂志文章,评审

    doi:10.1080/00313020212469

    authors: Garland SM

    更新日期:2002-06-01 00:00:00

  • Utility of B-type natriuretic peptides and cardiac troponins for population screening regarding cardiac abnormalities.

    abstract:AIMS:The increasing importance of cardiac disease has generated an interest in improved screening strategies regarding preclinical cardiac abnormalities and employing measurement of circulating biomarkers. This review focuses on the utility of the B-type natriuretic peptides (NP) and the cardiac troponins (cTns) for th...

    journal_title:Pathology

    pub_type: 杂志文章,评审

    doi:10.1097/PAT.0b013e32834e786e

    authors: Eggers KM,Venge P

    更新日期:2012-02-01 00:00:00

  • Angiotropic malignant melanoma and extravascular migratory metastasis: description of 36 cases with emphasis on a new mechanism of tumour spread.

    abstract:AIMS:We have identified in malignant melanoma an angio-tumoural complex in which tumour cells occupy a pericytic location along the endothelium of microvessels without evidence of intravasation. This pericytic angiotropism of melanoma cells, without any sign of intravasation, suggests that melanoma cells may migrate al...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.1080/00313020412331282708

    authors: Barnhill RL,Lugassy C

    更新日期:2004-10-01 00:00:00

  • Aneuploidy in sclerosing adenosis may predict an increased risk of malignancy. A study using the AgNOR silver stain.

    abstract::Sclerosing adenosis (SA) of the breast if a disease associated with an increase relative risk of malignancy. The exact incidence and factors influencing malignant transformation in association with SA are not well established. Two counts of the AgNOR silver staining technique have been correlated with ploidy and proli...

    journal_title:Pathology

    pub_type: 临床试验,杂志文章

    doi:10.1080/00313029700169015

    authors: Mourad WA

    更新日期:1997-08-01 00:00:00

  • Relations of TGF-beta1 with HIF-1 alpha, GLUT-1 and longer survival of colorectal cancer patients.

    abstract:AIMS AND METHODS:During colorectal carcinogenesis, transforming growth factor beta 1 (TGF-beta1) undergoes a functional change from suppression of cancer cell proliferation to inhibition of T cell mediated anti-cancer immunity. We aimed to evaluate relations among TGF-beta1 and cancer cell survival factors hypoxia indu...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.1080/00313020802579318

    authors: Sulkowska M,Wincewicz A,Sulkowski S,Koda M,Kanczuga-Koda L

    更新日期:2009-01-01 00:00:00

  • The perinatal autopsy: a contemporary approach.

    abstract::As autopsy rates in general hospitals decline, interest in the perinatal autopsy continues to rise, reflecting the emergence of a vigorous specialty growing in parallel with fetal medicine, prenatal diagnosis and clinical genetics. Perinatal autopsies are best carried out in tertiary centres which provide these servic...

    journal_title:Pathology

    pub_type: 杂志文章,评审

    doi:10.3109/00313029209063622

    authors: Chambers HM

    更新日期:1992-04-01 00:00:00

  • Necropsy findings in childhood leukaemia, emphasizing neutropenic enterocolitis and cerebral calcification.

    abstract::In 50 necropsies on leukaemic children, the major cause of death was infection. In patients dying during therapy for induction or reinduction of remission, the most frequent infection was a distinctive neutropenic enterocolitis or typhlitis. This was seen in 46% of the whole series and was a major factor in the death ...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.3109/00313027609059005

    authors: Moir DH,Bale PM

    更新日期:1976-07-01 00:00:00

  • Lithium-induced lung toxicity in rats: the effect of caffeic acid phenethyl ester (CAPE).

    abstract:AIMS:We aimed to evaluate the effects of caffeic acid phenethyl ester (CAPE) on lithium (Li)-induced lung toxicity. METHODS:Twenty-two adult male Wistar albino rats weighing between 280 and 300 g were used. The rats were randomly divided into three groups: control, Li and Li+CAPE groups. Li and CAPE were co-administer...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.1080/00313020500464904

    authors: Sahin O,Sulak O,Yavuz Y,Uz E,Eren I,Ramazan Yilmaz H,Malas MA,Altuntas I,Songur A

    更新日期:2006-02-01 00:00:00

  • Decreased expression of Bax-interacting factor-1 (Bif-1) in invasive urinary bladder and gallbladder cancers.

    abstract:AIMS:Mounting evidence indicates that deregulation of apoptosis is involved in the mechanisms of cancer development. Bax-interacting factor-1 (Bif-1) interacts with both Bax and Bak that are crucial for the intrinsic apoptosis signalling. Functionally, loss of Bif-1 expression has been proven to enhance tumorigenesis. ...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.1080/00313020802320440

    authors: Kim SY,Oh YL,Kim KM,Jeong EG,Kim MS,Yoo NJ,Lee SH

    更新日期:2008-10-01 00:00:00

  • Histopathology of the stapes in otosclerosis.

    abstract::A histopathological examination was made of 60 stapes obtained during surgery for clinical otosclerosis. The findings, correlated with the clinical history and macroscopic appearances of the stapes, led to the following observations. The histological extent of the focus of otosclerosis agrees well with the macroscopic...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.3109/00313028409067908

    authors: Iyer PV,Gristwood RE

    更新日期:1984-01-01 00:00:00

  • Low molecular weight IgM in the sera of patients with chronic lymphocytic leukemia.

    abstract::With the use of 2 independent techniques, circulating monomeric and oligomeric IgM were detected in the majority of 29 patients with chronic lymphocytic leukemia (CLL). In contrast it was detected only in trace quantities in a minority of healthy subjects. In the CLL group no significant correlation was observed betwe...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.3109/00313029309068902

    authors: Xu HJ,Roberts-Thomson PJ

    更新日期:1993-01-01 00:00:00

  • Langerhans cell histiocytosis: a comprehensive review.

    abstract::Langerhans cell histiocytosis (LCH) is currently regarded as a myeloid neoplasm, with remarkably broad clinical spectrum, ranging from isolated skin or bone lesions to a disseminated disease that can involve nearly any organ. LCH is generally regarded as a sporadic disease that occurs predominantly in the paediatric p...

    journal_title:Pathology

    pub_type: 杂志文章,评审

    doi:10.1097/PAT.0000000000000256

    authors: El Demellawy D,Young JL,de Nanassy J,Chernetsova E,Nasr A

    更新日期:2015-06-01 00:00:00

  • Experimental autoimmune glomerulonephritis in the rat. II. A histopathological and fine structural study.

    abstract::Human glomerular basement membrane (GBM) emulsified in Freund's complete adjuvant was injected into 16 Holtzman rats. Eleven (69%) of them subsequently developed glomerulonephritis. This disease was characterized by glomerular changes which included focal lobular hypercellularity due to localized hyperplasia of intrac...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.3109/00313027609094437

    authors: McCausland IP,Herdson PB,Gavin JB

    更新日期:1976-04-01 00:00:00

  • Reversed polarity of the glandular epithelial cells in micropapillary carcinoma of the large intestine and the EMA/MUC1 immunostain.

    abstract::Micropapillary carcinoma of the colon and rectum is associated with an adverse prognosis. This tumour type displays reverse polarity of the tumour cells and is stated to be characterised by an inside-out epithelial membrane antigen (EMA)/MUC1 staining. Nine cases of primary colorectal carcinoma and one omental metasta...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.1097/PAT.0000000000000144

    authors: Cserni G

    更新日期:2014-10-01 00:00:00

  • A variant of gelatinous transformation of marrow in leukemic patients post-chemotherapy.

    abstract::A variant of gelatinous transformation of marrow was described in leukemic patients post-chemotherapy. This lesion was found in 8 out of 1833 post-chemotherapy bone marrows from 429 patients with leukemia. Histologically, this variant form was identical to the classical gelatinous transformation except for the absence...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.3109/00313029309066592

    authors: Feng CS

    更新日期:1993-07-01 00:00:00

  • Immunohistochemistry for human telomerase reverse transcriptase catalytic subunit (hTERT): a study of 143 benign and malignant soft tissue and bone tumours.

    abstract:AIMS:Only a small number of mesenchymal tumours have been previously evaluated for hTERT expression. We hypothesised that hTERT expression would be frequently present in sarcomas, but not in benign tumours. METHODS:Sections from 143 bone/soft tissue tumours were immunostained for hTERT (44F12, 1:20; Novocastra) using ...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.1080/00313020903121614

    authors: Patel RM,Folpe AL

    更新日期:2009-01-01 00:00:00

  • Cancer stem cell markers in prostate cancer: an immunohistochemical study of ALDH1, SOX2 and EZH2.

    abstract::The aims of this study were to investigate the immunohistochemical expression and potential prognostic significance of putative cancer stems cell markers ALDH1, EZH2 and SOX2 in prostate cancer.A total of 142 consecutive radical prostatectomies submitted to one laboratory with a diagnosis of prostatic adenocarcinoma b...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.1097/PAT.0000000000000325

    authors: Matsika A,Srinivasan B,Day C,Mader SA,Kiernan DM,Broomfield A,Fu J,Hooper JD,Kench JG,Samaratunga H

    更新日期:2015-12-01 00:00:00

  • Granulomatous mastitis: a clinicopathological review of 26 cases.

    abstract:AIMS:Twenty-six cases of granulomatous mastitis were examined to determine the common histological and clinical features and the possible association with micro-organisms. METHODS:A retrospective review of the clinical and histological features of these cases was undertaken, including granuloma, inflammatory cell infi...

    journal_title:Pathology

    pub_type: 杂志文章,评审

    doi:10.1080/00313020410001692602

    authors: Tse GM,Poon CS,Ramachandram K,Ma TK,Pang LM,Law BK,Chu WC,Tang AP,Cheung HS

    更新日期:2004-06-01 00:00:00

  • Expression of placental proteins in complete and partial hydatidiform moles.

    abstract::Examination of routinely stained haematoxylin and eosin sections may sometimes prove inadequate in differentiating partial hydatidiform moles (PHM) from complete hydatidiform moles (CHM). While cytogenetic analysis can aid in the distinction, such facilities are not always available. The possibility of using immunohis...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.1080/00313029400169311

    authors: Cheah PL,Looi LM

    更新日期:1994-04-01 00:00:00

  • Hodgkin lymphoma: a review of pathological features and recent advances in pathogenesis.

    abstract::Hodgkin lymphoma (HL) is composed of two distinct pathological entities, nodular lymphocyte predominant HL and classic HL, the latter with four subtypes. In contrast with most other human lymphomas, in which the neoplastic cells are a major population of the tumour constituents, the neoplastic 'Hodgkin (H) and Reed-St...

    journal_title:Pathology

    pub_type: 杂志文章,评审

    doi:10.1016/j.pathol.2019.09.005

    authors: Piris MA,Medeiros LJ,Chang KC

    更新日期:2020-01-01 00:00:00

  • Multiple hepatic abscesses due to Yersinia enterocolitica.

    abstract::We report a case of multiple hepatic abscesses due to Yersinia enterocolitica in a 58 yr old male. This case is typical of the other 39 cases reported in the literature in that the patient had alcoholism, malnutrition, iron overload and a subsequently diagnosed malignancy. Ultrasound showed multiple hypoechoic areas i...

    journal_title:Pathology

    pub_type: 杂志文章,评审

    doi:10.1080/00313029500169353

    authors: Strungs I,Farrell DJ,Matar LD,Dekker L,Franz RJ

    更新日期:1995-10-01 00:00:00

  • Typing early Australian healthcare-associated MRSA: confirmation of major clones and emergence of ST1-MRSA-IV and novel ST2249-MRSA-III.

    abstract:AIMS:To investigate the evolutionary origins of Australian healthcare-associated (HCA) methicillin-resistant Staphylococcus aureus (MRSA) strains from a panel of historical isolates typed using current genotyping techniques. METHODS:Nineteen MRSA isolates from 1965 to 1981 were examined and antibiotic susceptibility p...

    journal_title:Pathology

    pub_type: 杂志文章

    doi:10.1097/PAT.0b013e3283632667

    authors: Lancashire JF,Jones A,Bergh H,Huygens F,Nimmo GR

    更新日期:2013-08-01 00:00:00