Abstract:
:Externalization of phosphatidylserine (PS) is thought to contribute to sickle cell disease (SCD) pathophysiology. The red blood cell (RBC) aminophospholipid translocase (APLT) mediates the transport of PS from the outer to the inner RBC membrane leaflet to maintain an asymmetric distribution of PL, while phospholipid scramblase (PLSCR) equilibrates PL across the RBC membrane, promoting PS externalization. We previously identified an association between PS externalization level and PLSCR activity in sickle RBC under basal conditions. Other studies showed that activation of protein kinase C (PKC) by PMA (phorbol-12-myristate-13-acetate) causes increased external PS on RBC. Therefore, we hypothesized that PMA-activated PKC stimulates PLSCR activity in RBC and thereby contributes to increased PS externalization. In the current studies, we show that PMA treatment causes immediate and variable PLSCR activation and subsequent PS externalization in control and sickle RBC. While TfR+ sickle reticulocytes display some endogenous PLSCR activity, we observed a robust activation of PLSCR in sickle reticulocytes treated with PMA. The PKC inhibitor, chelerythrine (Chel), significantly inhibited PMA-dependent PLSCR activation and PS externalization. Chel also inhibited endogenous PLSCR activity in sickle reticulocytes. These data provide evidence that PKC mediates PS externalization in RBC through activation of PLSCR.
journal_name
Eur J Haematoljournal_title
European journal of haematologyauthors
Barber LA,Palascak MB,Qi X,Joiner CH,Franco RSdoi
10.1111/ejh.12506subject
Has Abstractpub_date
2015-11-01 00:00:00pages
405-10issue
5eissn
0902-4441issn
1600-0609journal_volume
95pub_type
杂志文章abstract::Anaemia of inflammation (AI) is a frequent complication in patients suffering from chronic inflammatory disorders including infections, autoimmune and malignant disease. Cytokine imbalance with a shift towards T-helper (Th)1-type immune response seems to be important in the pathogenesis of this type of anaemia. Interf...
journal_title:European journal of haematology
pub_type: 杂志文章
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pub_type: 临床试验,杂志文章,多中心研究
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journal_title:European journal of haematology
pub_type: 杂志文章
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更新日期:1995-09-01 00:00:00
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更新日期:2015-05-01 00:00:00
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journal_title:European journal of haematology
pub_type: 杂志文章
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journal_title:European journal of haematology
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journal_title:European journal of haematology
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pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2004-11-01 00:00:00
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journal_title:European journal of haematology
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journal_title:European journal of haematology
pub_type: 杂志文章
doi:10.1111/ejh.12036
更新日期:2013-02-01 00:00:00
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journal_title:European journal of haematology
pub_type: 杂志文章,评审
doi:10.1111/ejh.12479
更新日期:2015-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-03-01 00:00:00