Abstract:
:The beta-adrenergic agonist isoproterenol stimulates immunoreactive atrial natriuretic peptide (IR-ANP) secretion by superfused rat atria in vitro. beta-Adrenergic agonists alter the cellular handling of calcium, which culminates in a rise in the systolic calcium concentration. This is achieved by increasing calcium influx through voltage-dependent calcium channels and by increasing the storage pool of calcium in the sarcoplasmic reticulum (SR). We therefore asked the question whether isoproterenol-stimulated IR-ANP secretion was dependent on the protein kinase A-induced rise in systolic calcium or was due to a direct effect of protein kinase A activation. Isolated rat left atria paced at 3 Hz were superfused in vitro. IR-ANP secretion was determined by radioimmunoassay of timed collections of the superfusate. Superfusion with 0.1 microM isoproterenol or 0.5 mM dibutyryl cyclic AMP increased IR-ANP secretion twofold. Stimulated IR-ANP secretion was lowered to near baseline by lowering the buffer calcium concentration from 1.8 to 0.2 mM or by adding to the superfusate 10 microM nitrendipine (a calcium-channel blocker) or 1 microM ryanodine (an inhibitor of SR calcium release). Superfusion of nonbeating, electrically quiescent left atria with 0.1 microM isoproterenol failed to stimulate IR-ANP secretion. We conclude: 1) Isoproterenol-stimulated IR-ANP secretion is dependent on calcium influx through voltage-dependent calcium channels and on the release of calcium from the SR. 2) Enhanced calcium influx alone is not adequate to maintain isoproterenol-stimulated IR-ANP secretion. 3) The SR appears to be the primary source of calcium for isoproterenol-stimulated IR-ANP secretion.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Circ Resjournal_title
Circulation researchauthors
Schiebinger RJdoi
10.1161/01.res.65.3.600subject
Has Abstractpub_date
1989-09-01 00:00:00pages
600-6issue
3eissn
0009-7330issn
1524-4571journal_volume
65pub_type
杂志文章abstract::Atrial tissue expresses both connexin 40 (Cx40) and 43 (Cx43) proteins. To assess the relative roles of Cx40 and Cx43 in atrial electrical propagation, we synthesized cultured strands of atrial myocytes derived from mice with genetic deficiency in Cx40 or Cx43 expression and measured propagation velocity (PV) by high-...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000250607.34498.b4
更新日期:2006-11-24 00:00:00
abstract::A number of scavengers of reactive oxygen metabolites reduce myocardial injury when given before ischemia and reperfusion, but few, if any, have proven to be effective when given near the onset of reperfusion. This is particularly true when infarct size is measured after at least 48 hours of reperfusion, when the full...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.68.6.1652
更新日期:1991-06-01 00:00:00
abstract::By using excised postmortem hearts obtained from 15 mongrel dogs with the pericardium intact, we investigated mechanical interactions between the four heart chambers from the standpoint of ventricular pressure-volume relationships. The interactions investigated were those between (1) the atrium and the ventricle, (2) ...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.50.1.86
更新日期:1982-01-01 00:00:00
abstract::Basic calcium phosphate (BCP) crystal deposition underlies the development of arterial calcification. Inflammatory macrophages colocalize with BCP deposits in developing atherosclerotic lesions and in vitro can promote calcification through the release of TNF alpha. Here we have investigated whether BCP crystals can e...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000171451.88616.c2
更新日期:2005-06-24 00:00:00
abstract::Multiple voltage-gated K+ channels contribute to the repolarization phases of the cardiac action potential and are targets of several antiarrhythmic drugs. The Kv1.5 K+ channel gene is expressed in the heart, and heterologous expression of this gene generates a slowly inactivating K+ current. Previously, we found that...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.75.6.1006
更新日期:1994-12-01 00:00:00
abstract::Angiotensin II (Ang II) increases renal sympathetic nerve activity in anesthetized mice before and after ganglionic blockade, suggesting that Ang II may directly activate postganglionic sympathetic neurons. The present study directly tested this hypothesis in vitro. Neurons were dissociated from aortic-renal and celia...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/hh0801.089542
更新日期:2001-04-27 00:00:00
abstract::The potential for heparin to enhance the training-induced increase in collateral-dependent blood flow to the distal hind-limb muscles was evaluated after bilateral femoral artery ligation in adult male rats (approximately 350 g). Rats received either saline (n = 34) or heparin (n = 36) injections and were kept sedenta...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.76.3.448
更新日期:1995-03-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.71.4.870
更新日期:1992-10-01 00:00:00
abstract::The effects of Bay K 8644 on the high-threshold calcium channel was investigated by means of the whole-cell patch-clamp technique in single guinea pig ventricular myocytes. The goal of the experiments was to characterize the inhibitory effects of Bay K 8644 on the calcium channel, and identify the factors that influen...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.62.1.97
更新日期:1988-01-01 00:00:00
abstract::A potent transcriptional enhancer was previously identified upstream of the mouse renin gene. Within the enhancer is a TGACCT direct-repeat motif, required for enhancer activity, that is the consensus sequence recognized by members of the thyroid hormone subfamily of steroid hormone receptors. We previously reported t...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000071355.82009.43
更新日期:2003-05-16 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000155964.34150.F7
更新日期:2005-02-18 00:00:00
abstract::Myocardial damage due to reperfusion of ischemic tissue is caused primarily by infiltrating neutrophils. Although leukocyte beta2 integrins (CD18) play a critical role, significant neutrophil emigration persists when CD18 is neutralized or absent. This study examined the role of leukocyte beta1 integrin (alpha4) and i...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.0000013835.53611.97
更新日期:2002-03-22 00:00:00
abstract::We have previously demonstrated pressure-dependent membrane depolarization and action potential generation in cat cerebral arteries. It was the purpose of this study to examine and compare the membrane electrical responses to increasing transmural pressure in spontaneously hypertensive rats with those of their normote...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.57.2.319
更新日期:1985-08-01 00:00:00
abstract::Recent advances in cardiac physiology identify the integrin-linked kinase (ILK) as an essential molecule regulating cardiac growth, contractility, and repair. A key transducer of biochemical signals initiated at the plasma membrane by cell-matrix interactions, ILK now emerges as a crucial player in mechanotransduction...
journal_title:Circulation research
pub_type: 杂志文章,评审
doi:10.1161/01.RES.0000265233.40455.62
更新日期:2007-05-25 00:00:00
abstract:RATIONALE:Activin receptor-like kinase-1 (ALK1) is an endothelial transforming growth factor β receptor involved in angiogenesis. ALK1 expression is high in the embryo vasculature, becoming less detectable in the quiescent endothelium of adult stages. However, ALK1 expression becomes rapidly increased after angiogenic ...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.112.275586
更新日期:2013-01-04 00:00:00
abstract:RATIONALE:In the exploratory Phase II STEM-AMI (Stem Cells Mobilization in Acute Myocardial Infarction) trial, we reported that early administration of G-CSF (granulocyte colony-stimulating factor), in patients with anterior ST-segment-elevation myocardial infarction and left ventricular (LV) dysfunction after successf...
journal_title:Circulation research
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1161/CIRCRESAHA.118.314617
更新日期:2019-07-19 00:00:00
abstract::Cyclic GMP modulates gene expression in vascular smooth muscle cells (SMCs) in part by stimulating cGMP-dependent protein kinase I (PKGI) and the phosphorylation of transcription factors. In some cells, cGMP increases nuclear translocation of PKGI and PKGI-dependent phosphorylation of transcription regulators; however...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.108.176321
更新日期:2008-07-03 00:00:00
abstract::The aim of this study was to examine the effect of exercise training on reflex control of vascular resistance in human males. Forearm vascular responses to lower body negative pressure (LBNP) at -10 and -40 mm Hg were compared between highly trained young athletes (21.5 +/- 0.5 years old, n = 14) and age-matched nonat...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.59.1.43
更新日期:1986-07-01 00:00:00
abstract::Animal and preliminary human studies of adult cell therapy following acute myocardial infarction have shown an overall improvement of cardiac function. Myocardial and vascular regeneration have been initially proposed as mechanisms of stem cell action. However, in many cases, the frequency of stem cell engraftment and...
journal_title:Circulation research
pub_type: 杂志文章,评审
doi:10.1161/CIRCRESAHA.108.176826
更新日期:2008-11-21 00:00:00
abstract::The sphingolipid metabolites, sphingosine (SPH), SPH 1-phosphate (S1P), and sphingosylphosphorylcholine (SPC), can act as intracellular as well as extracellular signaling molecules. These compounds have been implicated in the regulation of cell growth, differentiation, and programmed cell death in nonmyocytes, but the...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.85.11.1000
更新日期:1999-11-26 00:00:00
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pub_type: 杂志文章,评审
doi:10.1161/CIRCRESAHA.116.304678
更新日期:2015-06-05 00:00:00
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pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.110.219089
更新日期:2010-10-15 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is a critical regulator of angiogenesis that stimulates proliferation, migration, and proteolytic activity of endothelial cells. Although the mitogenic activity of VEGF is endothelial cell specific, recent reports indicate VEGF is able to stimulate chemotaxis and tissue factor...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.83.8.832
更新日期:1998-10-19 00:00:00
abstract:RATIONALE:Cardiac progenitor cells (CPCs) improve left ventricular remodeling and function after acute or chronic myocardial infarction. However, the long-term (>5 weeks) effects, potential tumorigenicity, and fate of transplanted CPCs are unknown. OBJECTIVE:To assess the outcome of CPC therapy at 1 year. METHODS AND...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.115.307647
更新日期:2016-04-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.46.1.11
更新日期:1980-01-01 00:00:00
abstract:RATIONALE:Increased sympathetic nerve activity has been linked to the pathogenesis of hypertension in humans and animal models. Enhanced peripheral chemoreceptor sensitivity which increases sympathetic nerve activity has been observed in established hypertension but has not been identified as a possible mechanism for i...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.109.206946
更新日期:2010-02-19 00:00:00
abstract::We studied the role of copper as a potential mediator of postischemic reperfusion injury in the isolated, perfused rat heart. Hearts were equilibrated with Krebs-Henseleit buffer for 10 minutes and then loaded with copper by way of perfusion with buffer containing 20 microM copper(II)-bis-histidial for 30 minutes. Con...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.69.3.881
更新日期:1991-09-01 00:00:00
abstract::A variety of delivery systems have been used to genetically modify vascular endothelial cells and smooth muscle cells (SMCs), but currently available systems suffer from either inefficient in vivo gene transfer, transient episomal vector expression, or significant immune responses and inflammation. In the present stud...
journal_title:Circulation research
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00
abstract::Heme oxygenase (HO)-1 is a protective antioxidant enzyme that prevents cardiomyocyte apoptosis, for instance, during progressive cardiomyopathy. Here we identify a fundamental aspect of the HO-1 protection mechanism by demonstrating that HO-1 activity in mouse heart stimulates the bigenomic mitochondrial biogenesis pr...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000338597.71702.ad
更新日期:2008-11-21 00:00:00
abstract::The mTOR (mechanistic target of rapamycin) is a master regulator of several crucial cellular processes, including protein synthesis, cellular growth, proliferation, autophagy, lysosomal function, and cell metabolism. mTOR interacts with specific adaptor proteins to form 2 multiprotein complexes, called mTORC1 (mTOR co...
journal_title:Circulation research
pub_type: 杂志文章,评审
doi:10.1161/CIRCRESAHA.117.311147
更新日期:2018-02-02 00:00:00