Abstract:
:The genome RNA and six mRNA species of infectious hematopoietic necrosis virus were analyzed by denaturing gel electrophoresis. The following molecular weights were determined: genome RNA, 3.7 X 10(6); mRNA 1, 2.26 X 10(6); mRNA 2, 5.63 X 10(5); mRNA 3, 4.84 X 10(5); mRNA 4 (containing two different mRNA species), 3.00 X 10(5); and mRNA 5, 1.95 X 10(5). Densitometer analyses of gels were used to calculate the molar ratios of the intracellular mRNA species: mRNA 1, 0.02; mRNA 2, 0.49; mRNA 3, 1.0; mRNA 4, 2.52; and mRNA 5, 0.41. Hybrid selection studies determined the mRNA coding assignments as follows: mRNA 1 encodes the viral polymerase, L; mRNA 2 encodes the glycoprotein, G; mRNA 3 encodes the nucleocapsid protein, N; mRNA 4 is composed of two comigrating mRNA species which encode the matrix proteins, M1 and M2; and mRNA 5 encodes a previously unrecognized viral protein which is induced in infected cells but is not present in mature virions. This nonvirion protein has been designated the NV protein.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kurath G,Leong JCdoi
10.1128/JVI.53.2.462-468.1985subject
Has Abstractpub_date
1985-02-01 00:00:00pages
462-8issue
2eissn
0022-538Xissn
1098-5514journal_volume
53pub_type
杂志文章abstract::Infection of cells with herpes simplex virus type 1 (HSV-1) induces high levels of deoxypyrimidine triphosphatase. The majority of the enzyme activity is found in infected cell nuclei. A similar activity is induced by HSV type 2 (HSV-2) which, in contrast to the HSV-1 enzyme, fractionates to more than 99% in the solub...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.3.935-942.1982
更新日期:1982-09-01 00:00:00
abstract::Eight nonoverlapping regions of the hemagglutinin (HA) molecule of influenza virus A/PR/8/34 (PR8), which serve as recognition sites for class II-restricted T cells (TH) from BALB/c mice, have been identified in the form of 10- to 15-amino-acid-long synthetic peptides. These TH determinants are located between residue...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.1.364-372.1991
更新日期:1991-01-01 00:00:00
abstract::Generating durable humoral immunity through vaccination depends upon effective interactions of follicular helper T (Tfh) cells with germinal center (GC) B cells. Th1 polarization of Tfh cells is an important process shaping the success of Tfh-GC B cell interactions by influencing costimulatory and cytokine-dependent T...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01737-19
更新日期:2020-02-28 00:00:00
abstract::The human immunodeficiency virus rev gene product regulates the expression of viral structural genes. It was recently shown that Rev regulates the export of viral structural mRNAs from the nucleus to the cytoplasm. Analysis of Rev subcellular localization reveals marked accumulation in the nucleolus, suggesting a role...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.881-885.1990
更新日期:1990-02-01 00:00:00
abstract::Viruses can induce progressive neurologic disorders associated with diverse pathological manifestations, and therefore, viral infection of the brain can impair differentiated neural functions, depending on the initial viral tropism. We have previously reported that canine distemper virus (CDV) targets certain mouse br...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.9.7317-7327.1999
更新日期:1999-09-01 00:00:00
abstract::Previous analysis of hepatitis B virus (HBV) indicated base pairing between two cis-acting sequences, the 5' half of the upper stem of epsilon and phi, contributes to the synthesis of minus-strand DNA. Our goal was to identify other cis-acting sequences on the pregenomic RNA (pgRNA) involved in the synthesis of minus-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01414-07
更新日期:2007-11-01 00:00:00
abstract::Fluorescent retroviral envelope (Env) proteins were developed for direct visualization of viral particles. By fusing the enhanced green fluorescent protein (eGFP) to the N terminus of the amphotropic 4070A envelope protein, extracellular presentation of eGFP was achieved. Viruses incorporated the modified Env protein ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.10.6070-6075.2003
更新日期:2003-05-01 00:00:00
abstract::Interleukin-1 receptor antagonist (IL-1ra) gene polymorphisms in 83 human immunodeficiency virus (HIV)-seropositive women were evaluated. Fourteen of the subjects (16.9%) were homozygous for IL-1ra allele 2 (IL-1RN*2). These women had a lower median level of HIV RNA than did women homozygous for allele 1 (IL-1RN*1) (P...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.13.6242-6244.2001
更新日期:2001-07-01 00:00:00
abstract::The infection of a bacterium by a phage starts with attachment to a receptor molecule on the host cell surface by the phage. Since receptor-phage interactions are crucial to successful infections, they are major determinants of phage host range and, by extension, of the broader effects that phages have on bacterial co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01559-20
更新日期:2020-11-09 00:00:00
abstract::Transformation-defective (td) mutants with different sizes of genomic RNA were isolated from the Prague strain of Rous sarcoma virus, subgroup C(PR-C). All six td viruses (tdTYPR-C) isolated from a single UV-irradiated stock of PR-C (clone 2 of TYPR-C) had slightly longer RNA than did the ordinary class b RNA of tdB77...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.2.453-461.1979
更新日期:1979-05-01 00:00:00
abstract::The production and selection of infectious vaccinia virus recombinants expressing foreign genes was facilitated by the construction of plasmid vectors. These vectors contain all or part of the vaccinia virus thymidine kinase (TK) gene interrupted by multiple unique restriction endonuclease sites placed adjacent to the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.3.857-864.1984
更新日期:1984-03-01 00:00:00
abstract::Wild-type clones of reovirus serotypes 1 (Lang), 2 (Jones), and 3 (Dearing) were serially passaged in L cells at a high multiplicity of infection, and the virus population was examined at passage levels 2, 5, and 11 for the presence of temperature-sensitive (ts) mutants. By passage 11 all three serotypes contained ts ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.34.1.285-287.1980
更新日期:1980-04-01 00:00:00
abstract::Epstein-Barr (EBV) is a powerful immortalizing virus for human B lymphocytes in vitro and is associated with several human neoplasias in vivo. Previously, we have shown that the majority of EBV-infected cells in the peripheral blood of healthy, persistently infected individuals do not express the activated phenotype, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.4882-4891.1997
更新日期:1997-07-01 00:00:00
abstract::Previous studies have established that chlorella viruses encode K(+) channels with different structural and functional properties. In the current study, we exploit the different sensitivities of these channels to Cs(+) to determine if the membrane depolarization observed during virus infection is caused by the activit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.5.2437-2444.2006
更新日期:2006-03-01 00:00:00
abstract::We isolated revertants of a polyomavirus whose origin of DNA replication contains a point mutation in the palindrome to which large T antigen binds. Four independent second-site revertants contain an Asp-286----Asn-286 substitution in large T antigen. This mutant large T antigen activates replication of DNAs containin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.1.242-249.1989
更新日期:1989-01-01 00:00:00
abstract::We have developed a system in which vesicular stomatitis virus (VSV) minigenomes encoding viral structural proteins can be expressed from plasmids. These RNAs can be replicated, transcribed, and packaged into infectious particles when coexpressed with the other VSV proteins. The minigenomes contain either the glycopro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.5.2946-2953.1995
更新日期:1995-05-01 00:00:00
abstract::Adenovirus infection affects the nuclear distribution of host splicing factors. Late phase-infected cells contain discrete clusters of small nuclear ribonucleoproteins (snRNPs) that are separate from centers containing the viral 72-kilodalton DNA-binding protein (72K protein). In the present study, we demonstrate that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.281-290.1995
更新日期:1995-01-01 00:00:00
abstract::Cottontail rabbit papillomavirus (CRPV)-induced papillomas progress at a high frequency to carcinomas and thus can serve as a model for high-cancer-risk human papillomavirus infection. Previously, we have shown that antibodies to nonstructural and structural proteins are detected in only a fraction of papilloma-bearin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.6.4043-4048.1994
更新日期:1994-06-01 00:00:00
abstract::Six phages were isolated from sewage and from a lysogenic strain. Three of them, belonging to a new morphological group, had contractile tails and elongated heads with axial ratios of 2.4:1. Two phages, possessing short tails and very long heads with axial ratios of 3.5:1, were new isolates of an extremely rare group....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.3.706-711.1974
更新日期:1974-03-01 00:00:00
abstract::The effect of human cytomegalovirus infection on cellular DNA synthesis in human fibroblasts was measured by fluorometry and by incorporation of radiolabeled thymidine. The results show that although HCMV infection stimulates cellular DNA synthesis in both quiescent and serum-stimulated cells, radiolabeled thymidine i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6402-6404.1996
更新日期:1996-09-01 00:00:00
abstract::Defective interfering RNAs have long been thought to be a causal factor of persistent RNA virus infections. Here we describe a novel type of defective genome of lymphocytic choriomeningitis virus and the unique mechanism by which these RNAs appear to contribute to the establishment and maintenance of persistent infect...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.9.6757-6764.1997
更新日期:1997-09-01 00:00:00
abstract::Human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) envelope (Env) proteins are extensively decorated with N-glycans, predominantly of the high-mannose type. However, it is unclear how high-mannose N-glycans on Env impact viral spread. We show that exclusive modification of SIV Env with these N-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01358-15
更新日期:2015-11-01 00:00:00
abstract:UNLABELLED:Recent evidence suggests that even in treated infections, human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) replication may continue in lymph nodes (LN), serving as a potential virus reservoir. Here we investigated the effects of lentivirus infection on natural killer (NK) cell frequ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00660-15
更新日期:2015-07-01 00:00:00
abstract::In various positive-sense single-stranded RNA viruses, a low-fidelity viral RNA-dependent RNA polymerase (RdRp) confers attenuated phenotypes by increasing the mutation frequency. We report a negative-sense single-stranded RNA virus RdRp mutant strain with a mutator phenotype. Based on structural data of RdRp, rationa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00834-19
更新日期:2019-10-29 00:00:00
abstract::A Staphylococcus aureus strain was successively cured of three prophages. A lysogenic conversion by a group A phage to production of Panton-Valentine leucocidin and by a group F phage to staphylokinase production could be demonstrated. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.2.318-319.1972
更新日期:1972-08-01 00:00:00
abstract::Assembly and maturation of retroviral particles requires the aggregation and controlled proteolytic cleavage of polyprotein core precursors by a precursor-encoded protease (PR). Active, mature retroviral PR is a dimer, and the accumulation of precursors at sites of assembly may facilitate subunit interaction and subse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.11.6165-6172.1991
更新日期:1991-11-01 00:00:00
abstract::Cytotoxic-T-lymphocyte (CTL) escape mutations undermine the durability of effective human immunodeficiency virus type 1 (HIV-1)-specific CD8(+) T cell responses. The rate of CTL escape from a given response is largely governed by the net of all escape-associated viral fitness costs and benefits. The observation that C...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03233-12
更新日期:2013-04-01 00:00:00
abstract::The D4R gene of vaccinia virus encodes a functional uracil-DNA glycosylase that is essential for viral viability (D. T. Stuart, C. Upton, M. A. Higman, E. G. Niles, and G. McFadden, J. Virol. 67:2503-2513, 1993), and a D4R mutant, ts4149, confers a conditional lethal defect in viral DNA replication (A. K. Millns, M. S...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.7965-7973.1996
更新日期:1996-11-01 00:00:00
abstract::The early steps of the intracellular trafficking of human papillomavirus type 16 (HPV-16), -31, and -58 pseudovirions were studied by investigating the effects of drugs acting at defined points of endocytosis pathways on virus-like particle-mediated pseudoinfection by overexpression of a dominant-negative form of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.6.3846-3850.2003
更新日期:2003-03-01 00:00:00
abstract::The relative contributions of interleukin-12 (IL-12) and IL-23 to viral pathogenesis have not been extensively studied. IL-12p40 mRNA rapidly increases after neurotropic coronavirus infection. Infection of mice defective in both IL-12 and IL-23 (p40(-/-)), in IL-12 alone (p35(-/-)), and in IL-23 alone (p19(-/-)) revea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00315-09
更新日期:2009-06-01 00:00:00