DNA grading of oral squamous carcinomas. A preliminary report.

Abstract:

:The prognostic validity of the DNA-malignancy-grade (DNA-MG), ranging on a continuous scale from 0.00 to 3.00 was tested in a preliminary study on 7 patients with oral squamous cell carcinomas. Monolayer smears were prepared after a cell separation procedure from paraffin-embedded surgical tumor specimens. Feulgen staining was performed automatically in a modified Shandon staining machine. A TV image analysis system with an automatic microscope (TAS plus, Leitz, FRG) was used for DNA measurements. The DNA-MG revealed a strong correlation with the patients prognosis. 3 patients who died after having a tumor relapse (mean survival = 10 months) had DNA grades greater than 1.40, whereas patients who survived without having a tumor relapse (mean survival = 53 months) revealed DNA grades below 1.00. Additionally, the DNA-malignancy-grade was closely correlated with the histopathologic malignancy grades.

authors

Chatelain R,Hoffmeister B,Härle F,Böcking A,Mittermayer C

doi

10.1016/s0901-5027(89)80015-3

subject

Has Abstract

pub_date

1989-02-01 00:00:00

pages

43-6

issue

1

eissn

0901-5027

issn

1399-0020

journal_volume

18

pub_type

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