Abstract:
:A thioalkylation procedure, which uses molecular sieves to promote the reaction, was exploited to provide peptides with useful functional groups (lipidic moieties), naturally occurring on proteins as post-translational modifications. The procedure was further implemented to synthesize tailor-made lipidated peptides, interesting tools to investigate biological processes involving their Ras parent proteins. Moreover, the one-pot preparation of multi-alkylated peptides confirms the versatility and flexibility of the employed methodology.
journal_name
Amino Acidsjournal_title
Amino acidsauthors
Calce E,Leone M,Monfregola L,De Luca Sdoi
10.1007/s00726-014-1742-ysubject
Has Abstractpub_date
2014-08-01 00:00:00pages
1899-905issue
8eissn
0939-4451issn
1438-2199journal_volume
46pub_type
杂志文章相关文献
AMINO ACIDS文献大全abstract::Ocular ischemia resulting from perfusion disturbances may play a major role in initiation of glaucoma. Possibly secondary to ischemia autoimmunogenic events are activated in glaucoma patients with increased prevalence of systemic autoimmune diseases. The determination of potential molecular markers in blood leukocytes...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-001-0141-3
更新日期:2002-01-01 00:00:00
abstract::Glutamate dehydrogenase (GDH) plays a crucial role in amino acid deamination and has been used as an inductor of nutrients metabolism. In this study, we cloned and analyzed the GDH cDNAs in diploids (red crucian carp), triploids and tetraploids and characterized their expression profiles upon dietary treatments. Resul...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1313-z
更新日期:2012-12-01 00:00:00
abstract::D-Aspartate (D-Asp) is a free D-amino acid detected in multiple brain regions and putative precursor of endogenous N-methyl-D-aspartate (NMDA) acting as agonist at NMDA receptors. In this study, we investigated whether D-Asp (20 mM) in drinking solution for 1 month affects pain responses and pain-related emotional, an...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-016-2205-4
更新日期:2016-07-01 00:00:00
abstract::Factor VIII, the plasma protein deficient or defective in individuals with hemophilia A, is a critical member of the blood coagulation cascade. Recent studies have identified the FVIII light chain region Glu1811-Lys1818 as being involved in FIXa binding and in the assembly of the FX-activating FIXaz-FVIIIa complex. Ba...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0464-z
更新日期:2010-07-01 00:00:00
abstract::Relaxin family peptides perform a variety of biological functions by binding and activating relaxin family peptide receptor 1-4 (RXFP1-4), four A-class G protein-coupled receptors. In the present work, we developed a novel ligand binding assay for RXFP3 and RXFP4 based on NanoLuc complementation technology (NanoBiT). ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-018-2588-5
更新日期:2018-08-01 00:00:00
abstract::Methionine (Met) is an essential amino acid for all organisms. In plants, Met also functions as a precursor of plant hormones, polyamines, and defense metabolites. The regulatory mechanism of Met biosynthesis is highly complex and, despite its great importance, remains unclear. To investigate how accumulation of Met i...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0562-y
更新日期:2010-10-01 00:00:00
abstract::G-protein-coupled receptors (GPCRs) are cell surface receptors. The dynamic property of receptor-receptor interactions in GPCRs modulates the kinetics of G-protein signaling and stability. In the present work, the structural and dynamic study of A(2A)R-D(2)R interactions was carried to acquire the understanding of the...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1218-x
更新日期:2012-10-01 00:00:00
abstract::This work deals with the Dakin-West synthesis, starting from the nucleoamino acid 1-thyminyl acetic acid, as well the NMR, ESI MS, and X-ray characterization of a heteroaromatic compound denominated by us T(2)CO, comprising two thymine moieties anchored to a 2-propanonic unit, the spectroscopic properties of which wer...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1237-7
更新日期:2012-10-01 00:00:00
abstract::Lateral diffusion of membrane-integral receptors within the plane of the membrane has been postulated to be mechanistically important for signal transduction. Direct measurement of polypeptide hormone receptor lateral mobility using fluorescence photobleaching recovery techniques indicates that tyrosine kinase recepto...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00805831
更新日期:1995-06-01 00:00:00
abstract::The presence of an enzyme activity which hydrolyzes glycyl-D-aspartate was found in the homogenates of pig kidney cortex. The activity was inhibited by metal chelating agents and cilastatin, suggesting that the enzyme was a cilastatin-sensitive metallo-peptidase. Of the two hydrolysis products,D-aspartate was found to...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00806591
更新日期:1996-06-01 00:00:00
abstract::Several descriptors of protein structure at the sequence and residue levels have been recently proposed. They are widely adopted in the analysis and prediction of structural and functional characteristics of proteins. Numerous in silico methods have been developed for sequence-based prediction of these descriptors. Ho...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0721-1
更新日期:2011-03-01 00:00:00
abstract::Skin is made up of mainly collagen type I and its structure is stabilised by the formation of covalent immature and mature crosslinks. In this study, collagen immature crosslink hydroxylysinonorleucine (HLNL) was isolated from bovine skin in high purity using two sequential purification steps. These consisted of prepa...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-019-02708-3
更新日期:2019-04-01 00:00:00
abstract::Skin collagen metabolism abnormalities induced by ultraviolet (UV) radiation are the major causes of skin photoaging. It has been shown that the one-time exposure of UV irradiation decreases procollagen mRNA expression in dermis and that chronic UV irradiation decreases collagen amounts and induces wrinkle formation. ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1059-z
更新日期:2012-06-01 00:00:00
abstract::The successful prediction of protein-folding rates based on the sequence-predicted secondary structure suggests that the folding rates might be predicted from sequence alone. To pursue this question, we directly predict the folding rates from amino acid sequences, which do not require any information on secondary or t...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1189-3
更新日期:2012-08-01 00:00:00
abstract::Dopa and related catecholamines and their degradation products have been demonstrated to have neurotoxic potential in a number of cellular and in vivo experiments. Several mechanisms have been hypothesized to be involved including generation of prooxidant products that subsequently oxidize membrane lipids and exposed ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF01345261
更新日期:1998-01-01 00:00:00
abstract::α-Pharmacological conotoxins are among the most selective ligands of nicotinic acetylcholine receptors with typical cysteine frameworks. They are characterized by the intercysteine loop and classified into various subfamilies, such as α3/5 and α4/7 conotoxins. A novel α-conotoxin, Pu14a (DCPPHPVPGMHKCVCLKTC), with a d...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1093-x
更新日期:2012-07-01 00:00:00
abstract::The reactions of phosphorus trichloride with various amino acids afford the pentacoordinated spirophosphoranes. The reaction procedures were traced by (31)P NMR spectra techniques. A new crystal structure of alanine derivative was characterized, which is a slightly distorted TBP structure. Besides, this kind of spirop...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-005-0207-8
更新日期:2005-06-01 00:00:00
abstract::Over the last few years, consistent data have demonstrated that creatine (Cr) supplementation prevents the accumulation of fat in rat liver as well as the progression of fatty liver disease in different situations. Studies have demonstrated that Cr is effective and prevents fatty liver in high-fat and choline-deficien...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-016-2183-6
更新日期:2016-08-01 00:00:00
abstract::The effects of metabotropic glutamate receptor (mGluR) activation were studied in medium spiny neurons and large aspiny (LA) interneurons by means of electrophysiological and optical recordings. DCG-IV and L-SOP, agonists for group II and III mGluRs, respectively, produced a presynaptic inhibitory effect on corticostr...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s007260070040
更新日期:2000-01-01 00:00:00
abstract::Oxidative stress, an excess of reactive oxygen species (ROS), may lead to oxidative post-translational modifications of proteins resulting in the cleavage of the peptide backbone, known as α-amidation, and formation of fragments such as peptide amides and α-ketoacyl peptides (α-KaP). In this study, we first compared d...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-020-02902-8
更新日期:2020-10-01 00:00:00
abstract::A simple and efficient one-pot procedure that enables rapid access to orthogonally protected N,N'-diaminoalkylated basic amino acid building blocks fully compatible with standard Boc and Fmoc solid-phase peptide synthesis is reported. Described synthetic approach includes double reductive alkylation of N (α)-protected...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1336-5
更新日期:2013-02-01 00:00:00
abstract::Amino acids are potent regulators of muscle protein synthesis and breakdown and have received considerable attention for the treatment of muscle wasting conditions. Arginine is critically involved in numerous physiological functions including providing substrate for the production of creatine, urea and nitric oxide (N...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-014-1815-y
更新日期:2014-12-01 00:00:00
abstract::We proposed that the combination of conjugated linoleic acid (CLA) and arginine would decrease adiposity by depressing lipid synthesis in liver and adipose tissues of growing pigs. Pigs were allotted to treatments in a 2 × 2 factorial design with two lipids (CLA or canola oil) and two amino acids [L-arginine or L-alan...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1255-5
更新日期:2012-10-01 00:00:00
abstract::Several new beta,beta-dihalo and beta-halo-beta-substituted dehydroalanines and dehydrodipeptides were synthesized by reacting the corresponding dehydroamino acid derivative with a N-halosuccinimide or in the case of beta,beta-di-iododehydroalanines with iodine. The results obtained confirmed that the stereochemical o...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0466-x
更新日期:2010-07-01 00:00:00
abstract::To evaluate the response of soybean to salt stress, the related changes in protein expression were investigated using the proteomic approach. Soybean plants were exposed to 0, 50, 100, and 200 mM NaCl. Especially at 200 mM, the length and fresh weight of the hypocotyl and root reduced under salt stress, while the prol...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-008-0036-7
更新日期:2009-01-01 00:00:00
abstract::The cross-linking of protein molecules to form stable supramolecular aggregates capable of acting as protective and supporting structures is a common feature of organisms coping with the stresses of life. These new polymeric forms range from thick rigid structures to thin flexible membranes. The formation of such cros...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00813999
更新日期:1991-10-01 00:00:00
abstract::Asymmetric dimethylarginine (ADMA) is a methylated form of arginine and an endogenous nitric oxide synthase inhibitor. Renal function decline is associated with increase of plasma ADMA in chronic kidney disease populations. It is yet unknown how isolated renal function impairment affects ADMA homeostasis in healthy hu...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-018-02693-z
更新日期:2019-03-01 00:00:00
abstract::Two experiments were conducted to evaluate the effects of dietary supplementation with Astragalus polysaccharide (APS) on growth performance, apparent ileal digestibilities (AID) of amino acids (AA), and their serum concentrations in early weaned piglets. In Exp. 1, 60 pigs were weaned at 21 days of age (BW 7.35 +/- 0...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-008-0142-6
更新日期:2009-07-01 00:00:00
abstract::Reports on nucleobase-containing chiral peptides (both natural and artificial) and achiral pseudopeptides are reviewed. Their synthesis, structural features, DNA and RNA-binding ability, as well as some other interesting applications which make them promising diagnostic/therapeutic agents of great importance in many a...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-010-0567-6
更新日期:2010-06-01 00:00:00
abstract::Filamentous fungi of the genusHypocrea were grown on malt extract/peptone agar, the mycelia were extracted with dichloromethane/methanol, and the extracts were totally hydrolyzed with 6 N HCl (110°C, 24 h). The amino acids (AA) released from peptides were converted into theirN(O)-pen-tafluoropropionyl 1-propyl esters ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00806923
更新日期:1991-06-01 00:00:00