Abstract:
OBJECTIVES:Recently marketed nucleic acid amplification tests (NAAT) for the detection of Neisseria gonorrhoeae (NG) have improved specificity over previous generation assays. A study to assess the necessity for confirmation of Roche cobas 4800 NG positive samples was undertaken by the Public Health Wales Microbiology Molecular Diagnostic Unit in Cardiff. METHODS:Classical NG culture identification was compared to cobas 4800 (DR-9), opacity (opa) gene and porA pseudogene (pap) results. Confirmatory NAATs (opa/pap) were performed prospectively for 120 cobas 4800 NG positive urogenital and extragenital samples. Retrospective supplementary NAAT and sequence analysis of additional cobas 4800 NG positive extragenital samples was also carried out. RESULTS:Of the 188 classically identified clinical NG isolates, 184 were identified as NG in all 3 molecular targets. Two isolates were only detected by 2 molecular targets. A further 2 isolates were culture false-positives. Combining the results from prospective and retrospective testing, the sensitivity and negative predictive value for cobas 4800 NG detection for urogenital, rectal and oropharyngeal samples was 100%. Specificity for all sample types was greater than 99.7%. Positive predictive value was 96.0% and 96.4% for urogenital and rectal specimens, respectively, and 88.6% for oropharyngeal samples. CONCLUSIONS:Molecular tests could be used for culture confirmation where available. Roche cobas 4800 Chlamydia trachomatis/Neisseria gonorrhoeae (CT/NG)CT/NG gonorrhoea diagnosis is superior to culture with urogenital and rectal positives not requiring confirmation. Roche cobas 4800 oropharyngeal NG detection findings warrant further prospective study of routine confirmatory testing accounting for its cost and clinical usefulness.
journal_name
Sex Transm Infectjournal_title
Sexually transmitted infectionsauthors
Perry MD,Jones RN,Corden SAdoi
10.1136/sextrans-2013-051410subject
Has Abstractpub_date
2014-06-01 00:00:00pages
303-8issue
4eissn
1368-4973issn
1472-3263pii
sextrans-2013-051410journal_volume
90pub_type
杂志文章abstract:OBJECTIVES:To compare endourethral swabs and urine as diagnostic specimens for the detection of genital Chlamydia trachomatis infection using the polymerase chain reaction (PCR), in male patients attending a genitourinary clinic and to assess whether the first endourethral swab used solely for diagnosing gonococcal inf...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.77.6.423
更新日期:2001-12-01 00:00:00
abstract:OBJECTIVE:From December 2017, the Australian National Cervical Screening Program commenced 5 yearly primary human papillomavirus (HPV) screening; one of the first high-income countries to implement primary HPV screening. This study aimed to examine the psychosocial impact of self-reporting testing HPV positive in a sam...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2019-054290
更新日期:2020-05-01 00:00:00
abstract:OBJECTIVE:Comprehensive data on Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) infections to guide screening services among transgender women (TGW) are limited. We studied the burden of CT/NG infections in pharyngeal, rectal and urethral sites of Thai TGW and determined missed CT/NG diagnoses if selected sit...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2018-053835
更新日期:2019-11-01 00:00:00
abstract::In industrialised countries, a rapid epidemic phase of HIV transmission has largely given way to more moderated endemic transmission. The dynamics of endemic transmission may differ substantially from those generating epidemic spread. We hypothesise that three elements play an important role in maintaining endemicity ...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,评审
doi:10.1136/sti.2006.017269
更新日期:2007-02-01 00:00:00
abstract:OBJECTIVES:To develop two new models of expedited partner therapy for the UK, and evaluate them for feasibility, acceptability and preliminary outcome estimates to inform the design of a randomised controlled trial (RCT). METHODS:Two models of expedited partner therapy (APTHotline and APTPharmacy), known as 'Accelerat...
journal_title:Sexually transmitted infections
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1136/sti.2010.047258
更新日期:2012-02-01 00:00:00
abstract:OBJECTIVES:HIV/AIDS risk is embodied within multiple levels including structural and social levels. The aim of this study was to assess the effects of neighbourhood characteristics on HIV prevalence among injection drug users (IDU) residing in the area of Tallinn, Estonia in 2007. METHODS:A cross-sectional, multilevel...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2009.040212
更新日期:2010-12-01 00:00:00
abstract:BACKGROUND:The objective of this study was to compare the costs and outcomes of two sexually transmitted infection (STI) screening interventions targeted at men in football club settings in England, including screening promoted by team captains. METHODS:A comparison of costs and outcomes was undertaken alongside a pil...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,随机对照试验
doi:10.1136/sextrans-2014-051715
更新日期:2015-03-01 00:00:00
abstract:OBJECTIVE:Three decades into the HIV epidemic and with the advancement of HIV treatments, condom and non-condom-based anal intercourse among gay men in resource-rich countries needs to be re-assessed. METHODS:The proportions of men engaging in a range of anal intercourse practices were estimated from the ongoing cross...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2011-050041
更新日期:2011-10-01 00:00:00
abstract:OBJECTIVE:To assess social patterns in human papillomavirus (HPV) vaccine coverage in a school-based, government-funded vaccination programme located within a single-payer universal healthcare system. DESIGN:We conducted a cross-sectional analysis of HPV vaccine uptake data for the 2013-2014 school year for 131 local ...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2017-053357
更新日期:2018-06-01 00:00:00
abstract:BACKGROUND:Serological testing for herpes simplex virus (HSV) type 2 in persons without a history of genital herpes is not recommended, partly because of concerns that an HSV-2 diagnosis would lead to negative psychosocial sequelae. This review aimed to examine the evidence regarding the psychosocial effects of HSV-2 s...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,评审
doi:10.1136/sextrans-2011-050099
更新日期:2011-12-01 00:00:00
abstract:OBJECTIVE:To investigate the characteristics of South African men who have sex with men (MSM) who (1) have been tested for HIV and (2) are HIV positive. METHODS:Data were collected from 1045 MSM in community surveys using questionnaires that were administered either face-to-face, by mail or on the internet. The mean a...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sti.2008.031500
更新日期:2008-11-01 00:00:00
abstract:OBJECTIVE:To determine the prevalence and genetic diversity of hepatitis C virus in genitourinary medicine clinic attenders and to assess the extent of sexual transmission of the virus. METHODS:A cross sectional, unlinked, anonymous survey in 14 genitourinary medicine clinics situated in England, Wales, and Northern I...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sti.79.4.301
更新日期:2003-08-01 00:00:00
abstract:OBJECTIVES:Oropharyngeal squamous cell carcinoma is the most common human papillomavirus (HPV)-associated cancer in the UK, but little is known about the prevalence of oropharyngeal HPV in sexually active teenagers. We investigated reported HPV vaccination coverage (in females) and prevalence of oropharyngeal HPV in se...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2020-054428
更新日期:2020-09-03 00:00:00
abstract:OBJECTIVES:To investigate the distribution of human papillomavirus (HPV) genotypes and determine the associations between HPV infection and HIV coinfection in sexually active heterosexual men with anogenital warts (GW), male urethral discharge or asymptomatic men. METHODS:Valid specimens for HPV genotyping were obtain...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sti.2009.037598
更新日期:2010-06-01 00:00:00
abstract:INTRODUCTION:In a systematic internet-based Chlamydia Screening Implementation Programme in The Netherlands, all chlamydia-positive participants automatically received a testkit after 6 months to facilitate early detection of repeat infections. The authors describe participation in repeat testing and prevalence and det...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2011-050455
更新日期:2013-02-01 00:00:00
abstract:BACKGROUND:Genital warts (condyloma acuminatum) remain one of the most commonly reported sexually transmitted infections (STI) worldwide. Most genital warts are caused by non-oncogenic human papilloma virus. Recurrence is common and many patients receive several rounds of treatment. There are limited data in the litera...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2008.030999
更新日期:2009-04-01 00:00:00
abstract:OBJECTIVES:To better understand the epidemiology of Trichomonas vaginalis infection, we investigated the association between T vaginalis and demographic, clinical, microbiological and behavioural characteristics of patients presenting with genital discharges to a primary healthcare clinic in Johannesburg, South Africa....
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2013-051049
更新日期:2013-09-01 00:00:00
abstract:OBJECTIVE:To evaluate sexual behaviour in 17 year old girls, using data from a national survey on adolescent sexuality. METHOD:The study was based on two samples of 17 year olds, comprising 2% of the population born in 1973 and carried out in 1990. A school sample and a sample of school non-attenders were recruited in...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.76.2.98
更新日期:2000-04-01 00:00:00
abstract::This review will delineate performance characteristics and limitations, as far as they are known, of the new glycoprotein G based, type specific HSV serologies. Several of these tests have been FDA approved in the United States for use in adults. With the departure of Gull/Meridian from the HSV serology market, it is ...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,评审
doi:10.1136/sti.77.4.232
更新日期:2001-08-01 00:00:00
abstract:OBJECTIVES:To determine the prevalence of urethritis pathogens amongst male symptomatic urethritis (MUS) patients, genital ulcer (GUS) patients without urethritis symptoms and men requesting HIV testing at a voluntary counselling and testing (VCT) clinic. METHODS:A prospective study was conducted in Johannesburg, Sout...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2007.028464
更新日期:2008-08-01 00:00:00
abstract:OBJECTIVES:To assess the effectiveness of an internet-accessed STI (e-STI) testing and results service on testing uptake among young adults (16-30 years) who have never tested for STIs in London, England. METHODS:We conducted secondary analyses on data from a randomised controlled trial. In the trial, participants wer...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,随机对照试验
doi:10.1136/sextrans-2019-053992
更新日期:2019-12-01 00:00:00
abstract:OBJECTIVE:HIV transmission is ongoing among men who have sex with men (MSM) in the UK. Sex without a condom (condomless sex, CLS) is the main risk factor. We investigated the prevalence of and factors associated with types of CLS. METHODS:Cross-sectional questionnaire study in UK HIV clinics in 2011/2012 (ASTRA). MSM ...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sextrans-2016-053029
更新日期:2017-12-01 00:00:00
abstract:BACKGROUND:Almost half of all new HIV infections in Thailand occur among low-risk partners of people infected with HIV, so it is important to include people infected with HIV in prevention efforts. METHODS:Risk for HIV transmission was assessed among people with HIV attending routine care at the National Infectious Di...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sti.2008.032532
更新日期:2009-02-01 00:00:00
abstract:OBJECTIVES:The diagnosis and treatment of Chlamydia trachomatis infection is important in preventing persistent or recurrent infection. Expedited partner therapy (EPT) is the favoured and supported method for STI treatment of the Centers for Disease Control and Prevention when the provider cannot be assured that all re...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2016-052887
更新日期:2018-11-01 00:00:00
abstract:OBJECTIVES:To investigate local cellular immune responses in patients with acute urethritis. METHODS:We have established T cell lines from the urethral exudate and examined their phenotype by flow cytometry. As controls, T cell lines were cultured from first pass urine specimens of asymptomatic healthy individuals. R...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.74.4.279
更新日期:1998-08-01 00:00:00
abstract::Despite having the highest prevalence of any sexually transmitted infection (STI) globally, there is a dearth of data describing Trichomonas vaginalis (TV) incidence and prevalence in the general population. The lack of basic epidemiological data is an obstacle to addressing the epidemic. Once considered a nuisance in...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,评审
doi:10.1136/sextrans-2013-051075
更新日期:2013-09-01 00:00:00
abstract:OBJECTIVES:To collect and analyse published and unpublished surveillance and research data on the prevalence of same sex sexual activity among male adults (including male-to-female transgenders and sex workers) in low and middle income countries. METHODS:Key indicators were operationalised (ever sex with a man, sex wi...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,评审
doi:10.1136/sti.2005.019489
更新日期:2006-06-01 00:00:00
abstract:BACKGROUND:Identifying predictors that contribute to geographical disparities in sexually transmitted infections (STIs) is necessary. This study assesses the spatial relationship between condom availability to locations of STIs in order to better understand these geographical disparities. OBJECTIVES:We conducted a con...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2015-052144
更新日期:2016-05-01 00:00:00
abstract:OBJECTIVES:To assess the feasibility and outcomes of recalling men who have sex with men (MSM) diagnosed as having a bacterial sexually transmitted infection (STI) for re-screening. METHODS:This evaluation was conducted from December 2008 for a 9-month period. MSM diagnosed as having a bacterial STI in that period wer...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2011-050144
更新日期:2011-12-01 00:00:00
abstract:OBJECTIVES:This study examines violence experienced in work and personal contexts and relation to HIV risk factors in these contexts among female sex workers (FSW) in Andhra Pradesh, India. METHODS:FSW at least 18 years of age (n=2335) were recruited through three rounds of respondent-driven sampling between 2006 and ...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2015-052162
更新日期:2016-09-01 00:00:00