Isolation and characterisation of T lymphocytes from the urethra of patients with acute urethritis.

Abstract:

OBJECTIVES:To investigate local cellular immune responses in patients with acute urethritis. METHODS:We have established T cell lines from the urethral exudate and examined their phenotype by flow cytometry. As controls, T cell lines were cultured from first pass urine specimens of asymptomatic healthy individuals. RESULTS:Using interleukin 2 (IL-2) alone a T cell line was obtained on only one occasion. Following culture with IL-2, and subsequent expansion by a single stimulation with irradiated allogenic peripheral blood mononuclear cells (PBMC), phytohaemagglutinin (PHA), and IL-2, it was possible to establish T cell lines from 6/6 acute urethritis patients. T cell lines were also obtained from 4/12 controls subjects, but required repetitive rounds of stimulation with mitogen and allogeneic PBMC to produce sufficient cell numbers for analysis. Three of the patient T cell lines were dominated by T cells expressing the gamma delta receptor. CONCLUSION:The gamma delta T cell subset has been associated with immune responses at mucosal surfaces and has the ability to recognise certain bacterial antigens. The gamma delta T cell response may represent an important aspect of the immune response to organisms associated with acute urethritis.

journal_name

Sex Transm Infect

authors

Brunst M,Shahmanesh M,Sukthankar A,Pearce JH,Gaston JS

doi

10.1136/sti.74.4.279

subject

Has Abstract

pub_date

1998-08-01 00:00:00

pages

279-83

issue

4

eissn

1368-4973

issn

1472-3263

journal_volume

74

pub_type

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