Abstract:
:Polymorphisms and somatic mutations in Flap Endonuclease 1 (FEN1), an essential enzyme involved in DNA replication and repair, can lead to functional deficiencies of the FEN1 protein and a predisposition to cancer. We identified a FEN1 germline mutation that changed residue E359 to K in a patient whose family had a history of breast cancer. We determined that the E359K mutation, which is in the protein-protein domain of FEN1, abolished the interaction of FEN1 with Werner syndrome protein (WRN), an interaction that is critical for resolving stalled DNA replication forks. Furthermore, although the flap endonuclease activity of FEN1 E359K was unaffected, it failed to resolve bubble structures, which require the FEN1 gap-dependent endonuclease activity. To determine the etiological significance of E359K, we established a mouse model containing this mutation. E359K mouse embryonic fibroblasts (MEF) were more sensitive to DNA crosslinking agents that cause replication forks to stall. Cytological analysis suggested that the FEN1-WRN interaction was also required for telomere stability; mutant cell lines had fragile telomeres, increased numbers of spontaneous chromosomal anomalies and higher frequencies of transformation. Moreover, the incidence of cancer was significantly higher in mice homozygous for FEN1 E359K than in wild-type mice, suggesting that the FEN1 E359K mutation is oncogenic.
journal_name
Oncogenejournal_title
Oncogeneauthors
Chung L,Onyango D,Guo Z,Jia P,Dai H,Liu S,Zhou M,Lin W,Pang I,Li H,Yuan YC,Huang Q,Zheng L,Lopes J,Nicolas A,Chai W,Raz D,Reckamp KL,Shen Bdoi
10.1038/onc.2014.19subject
Has Abstractpub_date
2015-02-12 00:00:00pages
902-11issue
7eissn
0950-9232issn
1476-5594pii
onc201419journal_volume
34pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::Cellular senescence functions as a tumor suppressor that protects against cancer progression. α-Catulin, an α-catenin-related protein, is reported to have tumorigenic potential because it regulates the nuclear factor-κB (NF-κB) pathway, but little is known about its clinical relevance and the mechanism through which i...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.637
更新日期:2011-06-09 00:00:00
abstract::We have previously demonstrated that the expression of FGFR3 is frequently downregulated in colorectal carcinoma cells. Here we have shown that FGFR1 is overexpressed in colorectal carcinoma cells and the gene expressions between FGFR1 and FGFR3 are mutually exclusive. Moreover, we have also shown that the disruption ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208254
更新日期:2005-01-27 00:00:00
abstract::Class I HLA expression is low in neuroblastoma tumours and cell lines. We have recently mapped a modifier of methylation for HLA-C (MEMO-1) to chromosomal bands 1p35-36.1, a region deleted in many neuroblastomas. Hypomethylation of HLA-C is strongly correlated with allelic loss of the MEMO-1 locus. Here, we show that ...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1996-10-17 00:00:00
abstract::In breast cancer (BC) patients, local recurrences often arise in proximity of the surgical scar, suggesting that response to surgery may have a causative role. Radiotherapy (RT) after lumpectomy significantly reduces the risk of recurrence. We investigated the direct effects of surgery and of RT delivered intraoperati...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2016.23
更新日期:2016-09-15 00:00:00
abstract::Infection by human immunodeficiency virus type 1 (HIV-1) is characterized by progressive loss of various cell types, mainly CD4+ T lymphocytes. While a passive role for the virus in cell destruction is recognized, it does not account for the vast amount of cell death including those of uninfected "bystander' cells. Si...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
abstract::The viability of rat embryo cells immortalized by thermosensitive mutants of SV40 or polyoma Large T antigen is impaired at the non-permissive temperature thus demonstrating that the immortal phenotype is dominantly maintained by Large T antigens. We have observed that exposing these cells to the restrictive temperatu...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1994-11-01 00:00:00
abstract::The tax gene product of the human T-cell leukemia virus type I (HTLV-I) induces the nuclear expression and biological function of the NF-kappa B/Rel family of host transcription factors although the underlying mechanism remains unclear. In the present study, we demonstrate that Tax-mediated activation of NF-kappa B/Re...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1995-09-07 00:00:00
abstract::This study addresses the question of whether loss of p16INK4 expression contributes to the immortalization of human cells. In vitro immortalization usually proceeds through two phases. In the first phase (lifespan extension), cells continue proliferating and their telomeres continue shortening beyond the point at whic...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1996-09-19 00:00:00
abstract::In order to obtain insight into the parameters determining the subcellular localization of mutant and wild-type forms of p53, we analysed the subcellular distribution of p53 in four Balb/c mouse-derived cell lines ranging in their cellular phenotypes from normal (3T3), via minimal transformant (T3T3), to maximally tra...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1992-07-01 00:00:00
abstract::The cbl oncogene was first identified as part of a transforming retrovirus which arose in a mouse pre-B cell lymphoma. Its protein product, p120cbl, is cytoplasmic and has several distinctive domains including a highly basic region, a RING finger motif and a large proline-rich domain. A mutation to cbl in the 70Z/3 pr...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1995-10-19 00:00:00
abstract::E mu-bcl-2 transgenic mice bearing the bcl-2 proto-oncogene linked to the immunoglobulin enhancer (E mu) sporadically develop B or T cell lymphomas after a long latent period. To identify genes that play important roles in development of lymphoid malignancies, proviral insertional mutagenesis with Moloney murine leuke...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1995-11-02 00:00:00
abstract::The Rb protein is a tumor suppressor, which plays a pivotal role in the negative control of the cell cycle and in tumor progression. It has been shown that Rb protein (pRb) is responsible for a major G1 checkpoint, blocking S-phase entry and cell growth. The retinoblastoma family includes three members, Rb/p105, p107 ...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1209615
更新日期:2006-08-28 00:00:00
abstract::Medullary thyroid carcinoma (MTC) is a rare human tumor affecting the calcitonin-secreting c-cells of the thyroid. Here we report that two independent strains of transgenic mice expressing a Polyomavirus (Py) truncated middle-T antigen (deltaMT), consisting of the amino-terminal 304 amino acids, and the full length Py...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202578
更新日期:1999-04-08 00:00:00
abstract::Signature abnormalities in the cell cycle and apoptotic pathway have been identified in mantle cell lymphoma (MCL), affording the opportunity to develop targeted therapies. In this study, we tested a novel class of kinase inhibitors, styryl sulfones, which differ from prior cell cycle inhibitors in that they are not r...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210350
更新日期:2007-08-16 00:00:00
abstract::We previously reported that YY-1, a versatile transcription factor, regulates expression of glycophorin gene by binding to its locus control region-like region (Gp-LCR) in combination with E-box binding protein during murine erythroleukemia (MEL) cell differentiation. In the present work, we demonstrated that YY-1 and...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202026
更新日期:1998-08-27 00:00:00
abstract::Secreted frizzled related protein 1 (SFRP 1) is an antagonist of the transmembrane frizzled receptor, a component of the Wnt signaling pathway, and has been suggested to be a candidate tumor suppressor in several human malignancies. Since SFRP 1 is located at chromosome 8 p 11, where lung cancers also exhibit frequent...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208777
更新日期:2005-09-15 00:00:00
abstract::Endothelial cells expressing fibroblast growth factor receptor-1 (FGFR-1) migrate and proliferate in response to treatment with FGF. We analysed ligand-induced migration and proliferation of porcine aortic endothelial cells expressing wild-type FGFR-1, point-mutated Y766F FGFR-1, unable to activate phospholipase C-gam...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201936
更新日期:1998-07-23 00:00:00
abstract::We have cloned the rat homolog of the human leukocyte common antigen-related gene (LAR), which encodes a transmembrane receptor phosphotyrosine phosphatase, and raised antibodies against its protein product. We present evidence here for a processing event resulting in a two-chain structure of the mature receptor on th...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00
abstract::We have previously shown that the PLAG1 gene on chromosome 8q12 is consistently rearranged in pleomorphic adenomas of the salivary glands with t(3;8)(p21;q12) translocations. The t(3;8) results in promoter swapping between the PLAG1 gene, which encodes a novel zinc finger protein, and the constitutively expressed gene...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201660
更新日期:1998-03-01 00:00:00
abstract::Cancer reflects the progressive accumulation of genetic alterations and subsequent genetic instability of cells. Cytogenetic studies have demonstrated the importance of aneuploidy in differentiated thyroid cancer development. The pituitary tumour transforming gene (PTTG), also known as securin, is a mitotic checkpoint...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208659
更新日期:2005-07-14 00:00:00
abstract::In Xiphophorus the causative, primary cellular oncogene for melanoma formation has been assigned by classical genetics to a sex-chromosomal locus, designated Tu. Activation of Tu was proposed to be the result of the elimination of Tu-specific regulatory genes which normally suppress the transforming function in the no...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
abstract::Mouse embryonic stem (ES) cells are known to express D-type cyclins at very low levels and these levels increase dramatically during in vitro and in vivo differentiation. Here, we investigate some of the signalling pathways regulating expression of cyclin D1 and progression to S phase, the Ras/Extracellular signal-reg...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205728
更新日期:2002-08-15 00:00:00
abstract::To elucidate the mechanisms behind the high sensitivity of myxoid/round cell liposarcoma (MRCL) to trabectedin and the suggested selectivity for specific subtypes, we have developed and characterized three MRCL xenografts, namely ML017, ML015 and ML004 differing for the break point of the fusion gene FUS-CHOP, respect...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2013.462
更新日期:2014-10-30 00:00:00
abstract::Heat shock protein (Hsp)90 regulates many key pathways in oncogenesis, including Akt and mammalian target of rapamycin (mTOR). The strengths of disruption of Hsp90 in cancer therapy include their versatility in inhibiting a wide range of oncogenic pathways. The present study demonstrated that synuclein γ (SNCG) protec...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2014.126
更新日期:2015-04-30 00:00:00
abstract::Clinical and epidemiological data support a role for human papilloma virus (HPV) type 16 in the pathogenesis of cervical carcinoma. The W12 cell line contains HPV16 sequences, predominantly as a high copy number episome, and is immortalised in vitro, but non-tumourigenic. A morphologically distinct sub-line of the par...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract::The PLZF gene was identified first by its fusion with the retinoic acid receptor alpha gene in the t(11;17) translocation associated with a retinoic acid resistant form of acute promyelocytic leukemia (APL). It encodes a krüppel-like zinc finger protein with a POZ domain shared with a subset of regulatory proteins inc...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202043
更新日期:1998-05-14 00:00:00
abstract::Ras-GRF, a guanine-nucleotide exchange factor that activates Ras p21, was tested for its ability to couple to either tyrosine kinase or heterotrimeric G protein signal transduction pathways. Ras-GRF failed to bind the SH2 and SH3 containing adaptor protein Grb2, either in vitro or in vivo. Furthermore, Ras-GRF did not...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1995-05-18 00:00:00
abstract::Vascular endothelial growth factor (VEGF), an important angiogenic factor, regulates cell proliferation, differentiation, and apoptosis through activation of its tyrosine-kinase receptors, such as Flt-1 and Flk-1/Kdr. Human malignant mesothelioma cells (HMC), which have wild-type p53, express VEGF and exhibit cell gro...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205382
更新日期:2002-04-25 00:00:00
abstract::Overexpression of the c-Jun proto-oncogene in MCF7 breast cancer cells results in a variety of phenotype changes related to malignant progression including increased motility and invasion. Concurrent with these phenotypic effects are changes in the expression of multiple gene targets. We previously demonstrated that e...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205857
更新日期:2002-10-10 00:00:00
abstract::The progression and negative outcome of a variety of human carcinomas are intimately associated with aberrant activity of the c-Met oncogene. The underlying cause of this dysregulation, however, remains a subject of discussion, as the majority of cancer patients do not present with activating mutations in c-Met recept...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.586
更新日期:2011-04-28 00:00:00