Abstract：
:In vitro metabolic acidosis (Met) induces greater bone mineral resorption than respiratory acidosis (Resp). Met, but not Resp, inhibits osteoblasts which control many aspects of osteoclastic function. To determine whether at a similar decrement in extracellular pH, Met and Resp would induce different changes in intracellular pH (pHi) and/or intracellular calcium concentration ([Ca2+]i) of osteoblasts, we measured pHi and [Ca2+]i in an osteoblast-like rat osteosarcoma cell line (UMR-106). Cells were grown to confluence on glass slides and loaded with either 1.5 microM BCECF, for pHi, or 1.5 microM Fura-2, for [Ca2+]i, in control (Ctl; pH approximately 7.40, PCo2 approximately 40, [HCO3-] approximately 24) medium. The fluorescence ratio at excitation wavelengths of 502 and 440 nm was measured for pHi and at 340 and 380 nm for [Ca2+]i. Following a baseline scan in Ctl medium, cells were transferred to either Met (pH approximately 7.10, PCo2 approximately 40, [HCO3-] approximately 12), Resp (pH approximately 7.10, PCo2 approximately 80, [HCO3-] approximately 24) or Ctl conditions. Medium pH, PCo2 and [HCO3-] were held constant over the course of the experiment. Compared to Ctl, pHi was lower in Met (P < 0.001) and even lower in Resp (P < 0.001 vs. Met and vs. Ctl). These changes were maintained over the period of observation. Compared to Ctl, [Ca2+]i was higher in Met (P < 0.001) and even higher in Resp (P < 0.001 vs. Met and vs. Ctl) within 20 to 100 seconds. However, after 100 seconds [Ca2+]i was not different in the three groups.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Kidney Intjournal_title
Kidney internationalauthors
Ori Y,Lee SG,Krieger NS,Bushinsky DAdoi
10.1038/ki.1995.247subject
Has Abstractpub_date
1995-06-01 00:00:00pages
1790-6issue
6eissn
0085-2538issn
1523-1755pii
S0085-2538(15)59019-4journal_volume
47pub_type
杂志文章abstract：:Primary hyperoxaluria type I (PH I) is a congenital error of metabolism that can be manifested by an increased oxalate production, and ultimately result in kidney failure. After a combined liver/kidney transplantation, children with PH I have persistent excretion of oxalate that causes crystal formation in the urinary...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1998.00876.x
更新日期：1998-05-01 00:00:00
abstract：:Females across their lifespan and certain male populations are susceptible to urinary tract infections (UTI). The influence of female vs. male sex on UTI is incompletely understood, in part because preclinical modeling has been performed almost exclusively in female mice. Here, we employed established and new mouse mo...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2018.04.023
更新日期：2018-09-01 00:00:00
abstract：:Bone marrow (BM) cells are reported to contribute to the process of regeneration following myocardial infarction. The present study examined two independent clonal studies to determine the origin of bone marrow (BM)-derived cardiomyocytes. First, we transplanted single CD34(-)c-kit(+)Sca-1(+)lineage(-) side population...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1111/j.1523-1755.2005.00624.x
更新日期：2005-11-01 00:00:00
abstract：BACKGROUND:Tubulointerstitial inflammation and fibrosis are pathologic hallmarks of end-stage renal disease (ESRD). Here we have used DNA microarray technology to monitor the transcriptomic responses to murine unilateral ureteral obstruction (UUO) with a view to identifying molecular modulators of tubulointerstitial fi...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2003.00306.x
更新日期：2003-12-01 00:00:00
abstract：:Cyclosporine A (CsA) is a potent immunosuppressive drug that inhibits the transcription of several proinflammatory cytokines including interleukin-2. In contrast, CsA stimulates transcription of the pleuripotent cytokine, transforming growth factor-beta (TGF beta). Since the effect of CsA in transplant recipients is u...
journal_title：Kidney international
pub_type： 临床试验,杂志文章
doi：10.1038/ki.1996.479
更新日期：1996-11-01 00:00:00
abstract：:We report the first case of a mitochondrial DNA (mtDNA) deletion diagnosed by renal biopsy. An eight-year-old girl with megaloblastic anemia and severe growth retardation developed progressive renal insufficiency accompanied by partial Fanconi syndrome. Histologic examination of the renal biopsy disclosed nonspecific ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1994.181
更新日期：1994-05-01 00:00:00
abstract：:Colony stimulating factor-1 (CSF-1) is a chemoattractant and growth factor for macrophages. In autoimmune MRL-lpr mice, CSF-1 is detected in the circulation and there is an increase in CSF-1 transcripts and macrophages in the kidney. The purpose of this study was to establish whether the MRL-lpr kidney is responsible ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1996.9
更新日期：1996-01-01 00:00:00
abstract：:Reliable estimates of the long-term outcomes of acute kidney injury (AKI) are needed to inform clinical practice and guide allocation of health care resources. This systematic review and meta-analysis aimed to quantify the association between AKI and chronic kidney disease (CKD), end-stage kidney disease (ESKD), and d...
journal_title：Kidney international
pub_type： 杂志文章,meta分析
doi：10.1016/j.kint.2018.08.036
更新日期：2019-01-01 00:00:00
abstract：:A new study in dialysis patients demonstrates that more than one-third of rehospitalizations within 30 days of discharge occur within the first week, and these rehospitalizations are associated with worse survival. Imposing a penalty on the dialysis provider for these rehospitalizations, as mandated by the Hospital Re...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2017.04.046
更新日期：2017-10-01 00:00:00
abstract：:Vitamin D analogs may slow the progression of chronic kidney disease. Tan et al. report that paricalcitol has additive effects with an angiotensin-converting enzyme inhibitor (ACEI) in suppressing extracellular matrix expression, interstitial inflammation, and myofibroblast activation in obstructive nephropathy. Paric...
journal_title：Kidney international
pub_type： 评论,杂志文章,评审
doi：10.1038/ki.2009.390
更新日期：2009-12-01 00:00:00
abstract：:Zhang and co-workers report on the renoprotective role of the vitamin D receptor (VDR) in diabetic nephropathy using the method of streptozotocin-induced hyperglycemia in wild-type and VDR(-/-) mice. Also, experiments with cultured mesangial cells and podocytes confirm the effect of the active vitamin D metabolite 1,2...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/sj.ki.5002693
更新日期：2008-01-01 00:00:00
abstract：:IgA nephropathy (IgAN) often shows lesions morphologically identical with those of focal segmental glomerulosclerosis (FSGS). In order to determine the possible role of FSGS in IgAN lesions, we measured glomerular capsular adhesions, often the first step toward FSGS, in biopsies from 127 patients with IgAN, 100 with l...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2010.466
更新日期：2011-03-01 00:00:00
abstract：:In seven patients on maintenance hemodialysis, de novo recurrent renal stone formation was observed. In all patients, the underlying disease was glomerulonephritis, with or without the nephrotic syndrome. All patients had considerable persistent proteinuria. The stones consisted predominantly of protein, as revealed b...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1979.188
更新日期：1979-12-01 00:00:00
abstract：:Impaired olfactory function is a marker of neurologic dysfunction in the uremic state. Retained uremic toxins not adequately cleared with dialysis may undermine the integrity of the olfactory epithelium and olfactory bulb and malign central olfactory processing. The evidence suggests that only renal transplantation, w...
journal_title：Kidney international
pub_type： 评论,社论
doi：10.1038/ki.2011.219
更新日期：2011-10-01 00:00:00
abstract：:Erythropoiesis stimulating agent (ESA) treatment has been a major advance in the care of patients with kidney disease, resulting in reduced symptoms and blood transfusions, and improved quality of life. Recent studies, however, have indicated that attempts to treat to near normal Hgb targets may result in increased ri...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.2008.467
更新日期：2009-02-01 00:00:00
abstract：:Because of differences in case-mix across states, state-level case-mix-adjusted end-stage renal disease (ESRD) incident rates are reported in each United States Renal Data System Annual Data Report to make the across-state comparisons valid. The adjusted rates were estimated by the direct adjustment method, a widely u...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5000299
更新日期：2006-04-01 00:00:00
abstract：:In studying the metabolic effects of diet potassium (K+) variation in normal humans, we noted that varying diet K+ within its normal range influenced inorganic phosphorus (Pi) homeostasis and serum calcitriol (1,25-dihydroxyvitamin D) levels. In six men who ingested a constant whole-foods diet containing (per 70 kg bo...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1990.120
更新日期：1990-05-01 00:00:00
abstract：BACKGROUND:Adrenomedullin (ADM) exerts antiproliferative effects on rat mesangial cells in vitro and, therefore is a possible renoprotective agent. In contrast, nitric oxide (NO) is capable of exerting both cytoprotective and cytotoxic actions. It was the objective of the present study to examine whether NO stimulates ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2002.00330.x
更新日期：2002-05-01 00:00:00
abstract：:Congenital chloride diarrhea is due to mutations in the intestinal Cl(-)/HCO(3)(-) exchange (SLC26A3) which results in sodium chloride and fluid depletion leading to hypochloremic and hypokalemic metabolic alkalosis. Although treatment with sodium and potassium chloride offers protection from renal involvement in chil...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2008.401
更新日期：2008-10-01 00:00:00
abstract：:International clinical practice guidelines for the management of anemia in chronic kidney disease suggest target hemoglobin levels ≤ 11.5 g/dl (115 g/l), with individualized consideration of slightly higher hemoglobin targets to improve quality of life. An updated meta-analysis of randomized trials demonstrates no sig...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2016.03.001
更新日期：2016-05-01 00:00:00
abstract：:We sought to study glomerular pathophysiology in a model of IgA nephropathy (IgAN). Preliminary experiments with oral immunization indicated that Lewis rats had higher IgA levels and IgA/IgG ratios than Wistar, Fischer or Sprague-Dawley rats. Six groups of Lewis rats were studied: four continuously orally immunized fo...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1992.268
更新日期：1992-07-01 00:00:00
abstract：BACKGROUND:Hypertriglyceridemia is a result of reduced triglyceride (TG)-rich lipoprotein (TRL) catabolism and occurs in rats with nephrotic syndrome (NS) and in Nagase analbuminemic rats (NARs). While the heparin-releasable lipoprotein lipase (LpL) pool in NAR and in NS is similar, TG levels are significantly greater ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2001.00478.x
更新日期：2001-01-01 00:00:00
abstract：:Amylin (islet amyloid peptide) plays a critical role in islet amyloidosis and in the development of beta-cell dysfunction in patients with diabetes; however, the involvement of amylin in renal amyloidosis has not been studied. For this reason, we surveyed 149 patients with biopsy-proven diabetic nephropathy (DN). The ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5002305
更新日期：2007-07-01 00:00:00
abstract：:Podocyte foot process effacement is characteristic of proteinuric renal diseases. In minimal change nephrotic syndrome (MCNS) foot processes are diffusely effaced whereas the extent of effacement varies in focal segmental glomerulosclerosis (FSGS). Here we measured foot process effacement in FSGS and compared it to th...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2008.413
更新日期：2008-12-01 00:00:00
abstract：:Gap junctions are present in the juxtaglomerular apparatus enabling intercellular communication. Our study determined the location of different connexin subtypes within the juxtaglomerular apparatus of the rat, and the role of these subtypes in renal hemodynamics through the use of specific mimetic peptides. Immunohis...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5002673
更新日期：2008-02-01 00:00:00
abstract：BACKGROUND:The long-term prognostic associations of pre- and post-dialysis blood pressures, interdialytic weight gain, and antihypertensive use in hemodialysis patients are unclear. METHODS:The United States Renal Data System (USRDS) Dialysis Morbidity and Mortality Waves 3 and 4 Study, a randomly generated sample of ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2002.00636.x
更新日期：2002-11-01 00:00:00
abstract：:Human renal dysplasia is frequently associated with urinary tract obstruction and the abnormal expression of mitogen-activated protein kinase (MAPK). Here, we determined the renal responses and MAPK expression in developing kidneys that were obstructed in fetal lambs. Kidneys were harvested at various times after obst...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2008.3
更新日期：2008-05-01 00:00:00
abstract：:The Dietary Approaches to Stop Hypertension (DASH) diet lowers blood pressure, an important risk factor for chronic kidney disease (CKD) and end-stage renal disease (ESRD). However, it is unclear whether adherence to a DASH diet confers protection against future ESRD, especially among those with pre-existing CKD and h...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2018.12.027
更新日期：2019-06-01 00:00:00
abstract：:Starting from observations in intact cells and extending to studies in native membranes and solubilized membrane proteins, a significant body of evidence has been accumulated to indicate that some of the short-term regulatory influences on the Na+-H+ exchanger in the apical membrane of the proximal convoluted tubule a...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.1989.226
更新日期：1989-10-01 00:00:00
abstract：:Progressive renal vascular sclerosis is a key feature of chronic kidney disease (CKD). Adiponectin, an adipokine with potent anti-inflammatory and antiatherosclerotic properties, is associated with insulin resistance, type II diabetes and cardiovascular disease. In this study, we evaluated the predictive value of adip...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5002191
更新日期：2007-06-01 00:00:00
