Abstract：
:A method for analysis of the four stereoisomers of 1045U85 in rat plasma was developed and validated. The method involved liquid extraction of 1045U85 and an internal standard (propranolol) from plasma, followed by reaction with a chiral derivatizing reagent, GITC. The diastereoisomeric products were then separated by reversed-phase LC. The range of quantitation was 9.828-0.121 micrograms ml-1 for total 1045U85 (3.440-0.042 micrograms ml-1 for the RR and SS isomers, and 1.474-0.018 micrograms ml-1 for the RS and SR isomers). Specificity of the method for 1045U85 was demonstrated using spiked plasma samples as well as plasma samples from dosed animals. Extraction recovery of 1045U85 and propranolol was greater than 95%, and the derivatization reaction was shown to be complete. Accuracy (% bias) ranged from -2.6 to 3.9% for total 1045U85 and from -4.7 to 14.1% for the individual stereoisomers. Precision (% RSD) was 3.8-8.7% for total 1045U85 and 2.9-16.5% for the individual isomers. Plasma samples stored at -70 degrees C were stable for 19 weeks. The method has been used to determine plasma 1045U85 concentrations in nonclinical studies with this compound.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
McNulty MJ,Page TL,Deal DLdoi
10.1016/0731-7085(94)00077-8subject
Has Abstractpub_date
1994-11-01 00:00:00pages
1453-61issue
11eissn
0731-7085issn
1873-264Xpii
0731-7085(94)00077-8journal_volume
12pub_type
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
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更新日期：2010-01-05 00:00:00
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/0731-7085(96)01808-0
更新日期：1996-08-01 00:00:00
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/s0731-7085(96)01872-9
更新日期：1996-12-01 00:00:00
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 临床试验,杂志文章
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更新日期：1997-06-01 00:00:00
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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更新日期：2010-12-01 00:00:00
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journal_title：Journal of pharmaceutical and biomedical analysis
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