Abstract：
:Antioxidants are added to foods and drugs to inhibit their oxidation. As these additives are somewhat toxic, it is necessary to control the amount added to any food or drug. Liquid chromatography (LC) is a powerful tool for this purpose. Many antioxidants are electroactive molecules which enables the advantages of electrochemical detection or selectivity and sensitivity to be realized. The interest of analysts in microbore LC arises from the low mobile phase volumetric flow rates involved, the reduced on-column samples together with reduced chromatographic dilution and high efficiency. Coupling of microbore LC with electrochemical detection adds another advantage: the decrease of electrode ageing. The problem of extra-column band broadening with microbore column is discussed in the present communication. A micro LC-electrochemical detection system is constructed and tested using catecholamines. The limit of detection (LOD) for noradrenaline using a 0.7 mm bore column is found to be 0.1 pg injected in 0.2 microliter (0.6 femtomoles). Three phenolic antioxidants are studied: tert-butyl-p-hydroxyanisole (BHA), di-tert-butyl-hydroxytoluene (BHT) and n-propyl gallate (3,4,5-trimethoxybenzoic acid propyl ester). The dynamic range is four orders of magnitude with LODs down to 0.1 femtomoles (20 fg injected) with a 0.3 mm bore column. No electrode response change is observed after 60 injections of 3 ng BHA over 6 days. Antioxidants are determined in different pharmaceutical preparations and foodstuffs (chewing gums, dried potato flakes). The agreement between the manufacturer stated concentrations and observed results is found to be satisfactory.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Boussenadji R,Porthault M,Berthod Adoi
10.1016/0731-7085(93)80150-ysubject
Has Abstractpub_date
1993-01-01 00:00:00pages
71-8issue
1eissn
0731-7085issn
1873-264Xpii
0731-7085(93)80150-Yjournal_volume
11pub_type
杂志文章abstract：:The purpose of this study was to investigate whether antipyrine estimation in saliva provides valid information on plasma antipyrine clearance (APCl) and can be useful as an index of changes in drug metabolism with age. Antipyrine kinetics was studied in 93 elderly (mean age 82 years) and 23 young (mean age 29 years) ...
journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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pub_type： 杂志文章
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2008.05.033
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章,评审
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pub_type： 杂志文章
doi：10.1016/j.jpba.2013.10.041
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2010.06.024
更新日期：2010-12-01 00:00:00
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/s0731-7085(03)00231-0
更新日期：2003-08-21 00:00:00
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/s0731-7085(99)00245-9
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章,评审
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
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journal_title：Journal of pharmaceutical and biomedical analysis
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pub_type： 杂志文章
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/0731-7085(95)01307-7
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pub_type： 杂志文章,评审
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更新日期：2014-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：2020-08-05 00:00:00
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
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journal_title：Journal of pharmaceutical and biomedical analysis
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