Abstract:
:ICAM-1 is an inducible glycoprotein important in the adhesion, activation, and transmigration of circulating leukocytes across the vascular endothelial monolayer, and it likely plays a key role in the allogeneic response. To determine the reproducibility and significance of variations in resting levels of cell surface ICAM-1, 3 individual measurements of ICAM-1 levels were performed on 26 individual isolates of human aortic endothelial cells (HAECs) both at rest and following activation by allogeneic lymphocytes, using flow cytometry. Resting HAEC ICAM-1 levels varied 10-fold (range 6-60 mean fluorescence channels) depending on the isolate studied. There were strong correlations (r = 0.71 to 0.77, P < 0.0001) between the three measurements (performed no closer than weekly intervals on separate cultures), attesting to the consistency of the phenotypic expression. Constitutive expression of ICAM-1 was not affected by cell age, based upon comparing a subset of these isolates across 3 population doublings. Levels of HAEC ICAM-1 following allogeneic lymphocyte activation varied 15-fold (range 20-300 mean fluorescent channels) and, more important, correlated with resting ICAM-1 levels (r = 0.58, P = 0.002). Finally, constitutive ICAM-1 expression was related to TNF-alpha-induced ICAM-1 levels based upon a subset of the isolates studied. These data suggest that phenotypic, and likely genetic, differences in quiescent endothelial cell adhesion molecule expression can influence inflammatory responses including alloresponsiveness to the vasculature.
journal_name
Transplantationjournal_title
Transplantationauthors
Everett JP,Shipley GD,Mauck KA,Wagner CR,Morris TE,Hosenpud JDdoi
10.1097/00007890-199410270-00015subject
Has Abstractpub_date
1994-10-27 00:00:00pages
946-50issue
8eissn
0041-1337issn
1534-6080journal_volume
58pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract::The aim of our study was to sensitize cells in vitro, follow their proliferative and cytotoxic responses, and determine their ability to cause lethal graft-versus-host disease (GVHD). C57BL/6 (H2b) spleen cells were incubated with irradiated BALB/C (H2d) Moloney lymphoma cells (LSTRA) in mixed leukocyte culture condit...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197612000-00008
更新日期:1976-12-01 00:00:00
abstract:BACKGROUND:Perioperative renal dysfunction in liver transplant recipients complicates maintenance immunosuppressive therapy, particularly in patients with hepatitis C. Calcineurin inhibitors exacerbate renal dysfunction and mammalian target-of-rapamycin inhibitors are generally avoided because of perceived perioperativ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000438635.44461.2e
更新日期:2014-01-27 00:00:00
abstract:BACKGROUND:The functional relevance of HLA-C mismatches in an alloresponse is still much debated, putting into doubt the relevance of matching for this antigen in selection of an allogeneic bone marrow donor. In addition to presenting peptides to T cells, HLA-C also functions as a ligand for killing inhibitory receptor...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200109150-00030
更新日期:2001-09-15 00:00:00
abstract:BACKGROUND:The aging of liver transplant (LT) recipients, the weighting of the model for end-stage liver disease score, and the increased prevalence of nonalcoholic steatohepatitis has led to an increased number of older LT recipients with pre-LT chronic kidney disease (CKD). There are limited data on the impact of inc...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000003147
更新日期:2020-12-01 00:00:00
abstract:BACKGROUND:BK polyomavirus infection has been reported in 10% to 60% of renal transplant recipients with progression to BK nephropathy (BKN) occurring in 1% to 5% of patients. Graft loss occurs in up to 60% of renal transplant recipients with BKN. Because BK polyomavirus infection is believed, in part, to be a manifest...
journal_title:Transplantation
pub_type: 杂志文章,随机对照试验
doi:10.1097/TP.0b013e3181f2c94b
更新日期:2010-10-27 00:00:00
abstract:BACKGROUND:Previous studies have shown that posttransplant total lymphoid irradiation, anti-thymocyte globulin, and an intravenous donor blood cell infusion induce tolerance to ACI heart allografts in Lewis rat hosts. METHODS:In the current study, fresh ACI monocytes and dendritic cell precursors, derived from short-t...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199811270-00004
更新日期:1998-11-27 00:00:00
abstract::Forty-three human liver allograft biopsies and normal liver were directly analyzed for inflammatory and immunoregulatory cytokine gene expression by polymerase chain reaction (PCR). IL-5 gene expression was predominantly present in biopsies from liver allografts with histopathological evidence of acute rejection. IL-2...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199202010-00035
更新日期:1992-02-01 00:00:00
abstract::We performed a multicenter study to investigate the problem of cadaver kidney transplants that never achieve function, or that recover after a period of delayed function, and we used multivariate techniques to model the results. Sixty-nine patients who received cadaver kidney transplants that never functioned (NF) wer...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198801000-00027
更新日期:1988-01-01 00:00:00
abstract:BACKGROUND:To reduce the side effects of long-term immunosuppressive therapy, stable renal transplant patients were routinely converted from cyclosporine to either azathioprine or mycophenolate mofetil. Thereafter, the azathioprine and mycophenolate mofetil dose was reduced to 75% at 4 months and to 50% at 8 months aft...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:2000-07-15 00:00:00
abstract:BACKGROUND:In 2013, the public health service (PHS) changed the criteria intended to identify organ donors that put the associated organ recipients at increased risk for acquiring human immunodeficiency virus (HIV), hepatitis B virus (HBV), and hepatitis C virus (HCV). The changing donor demographics, organ utilization...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000001716
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:Macroencapsulated islets can reverse hyperglycemia in diabetic animals when transplanted i.p. or into the fat pad. The s.c. space is an attractive site for such transplantation because macrocapsules can be implanted with local anesthesia and be easily removed or reloaded with fresh islets. METHODS:Immunopro...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199903150-00005
更新日期:1999-03-15 00:00:00
abstract:BACKGROUND:Geographic variation in kidney transplantation rates in the United States has been described previously but remains unexplained by age, race, sex, or socioeconomic status differences. Geographic variations in the concentration of poverty appear to impact end-stage renal disease care and potentially access to...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000000125
更新日期:2014-09-27 00:00:00
abstract:BACKGROUND:Composite tissue allotransplantation (CTA) is a recently introduced option for limb replacement and reconstruction of other nonreconstructible tissue defects. As with recipients of other allotransplants, CTA recipients can experience rejection episodes that are presumed to be mediated by immune mechanisms si...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:2005-12-27 00:00:00
abstract::The mission of the recently established Immune Tolerance Network includes the development of protocols for the induction of transplant tolerance in organ allograft recipients and the development of assays that correlate with and may be predictive of the tolerant state. The state of clinical organ transplant tolerance ...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:
更新日期:2001-10-27 00:00:00
abstract::In an attempt to investigate the nonspecificity of the effect of administration of donor splenocytes treated with mitomycin C (MMC) 7 days before transplantation in inducing immunological unresponsiveness, the survival rates of liver, heart, small bowel, and skin allografts were compared in the RT1-incompatible ACI(RT...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198902000-00036
更新日期:1989-02-01 00:00:00
abstract::We reviewed coded autopsy material from 71 dogs, 46 receiving allogeneic and 25 receiving autologous bone marrow transplants, to evaluate the hypothesis that lymphocytic bronchitis is associated with severe acute graft-versus-host disease (GVHD). We found no significant correlation between the presence of lymphocytic ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198403000-00003
更新日期:1984-03-01 00:00:00
abstract:BACKGROUND:Detection of markers predicting allograft rejection is important for risk assessment before kidney transplantation, as well as to minimize posttransplantation immunosuppression. METHODS:We studied the expression of CD25, HLA-DR, CD134, CD62L, and CD44 by flow cytometry in CD4, CD8, and CD3 cells, from pretr...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3182283c58
更新日期:2011-09-15 00:00:00
abstract::Cytomegalovirus remains a significant source of morbidity and mortality in immunocompromised hosts. The increased sensitivity of molecular diagnostic techniques (PCR, antigenemia) has resulted in our ability to detect viral replication earlier in the posttransplant period, before the onset of symptoms. With the advent...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199701270-00017
更新日期:1997-01-27 00:00:00
abstract::The immunosuppressive effects of cyclosporin A were tested in a DA (RT-1a) to Lewis (RT-1(1) rat renal allograft model, which represents a very strong histocompatibility barrier. Dose-response studies established that oral doses of 5 mg/kg/day or higher gave complete suppression of rejection, while oral doses of 2 mg/...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198005000-00003
更新日期:1980-05-01 00:00:00
abstract::Orthotopic liver transplantation is frequently associated with a complex coagulation disorder, influencing the outcome of the procedure. In this respect, disseminated intravascular coagulation (DIC) had been suggested to be of causative importance for bleeding complications after reperfusion of the liver graft. In 10 ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199109000-00018
更新日期:1991-09-01 00:00:00
abstract:BACKGROUND:Organ transplantation has been successfully practiced for decades, but the outcome of cell transplantation remains disappointing. This is the case in animal models; liver allografts in mice are spontaneously accepted without requirement of immunosuppression, whereas hepatocyte transplants in the same combina...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31818bfd13
更新日期:2008-12-15 00:00:00
abstract:BACKGROUND:The role of human herpesvirus (HHV)-8 in the pathogenesis of multiple myeloma and its pre-malignant state of monoclonal gammopathy is unclear. HHV-8 is transmitted by organ transplantation, representing a unique model with which to investigate primary HHV-8 infection. METHODS:The authors studied the inciden...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000129065.31427.96
更新日期:2004-05-27 00:00:00
abstract::To evaluate the pharmacokinetic properties of the new microemulsion formulation of cyclosporine (Sandimmun Neoral), a double-blind, prospective study in stable renal transplant recipients was performed. The patients were randomized on a 4:1 basis either to receive Sandimmun Neoral (n = 45) or continue on regular Sandi...
journal_title:Transplantation
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1097/00007890-199510150-00005
更新日期:1995-10-15 00:00:00
abstract:BACKGROUND:We assess whether remote ischemic preconditioning (rIPC) of the recipient can modify ischemia-reperfusion (IR) injury in the donor heart following orthotopic heart transplantation from brain dead donors and to examine potential mechanisms of protection. METHODS:Sixteen pigs weighing from 26 to 34.2 (mean 29...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000159137.76400.5d
更新日期:2005-06-27 00:00:00
abstract::Acute rejection episodes still occur after kidney transplantation in spite of modern immunosuppressive protocols including combined tacrolimus, mycophenolate mofetil, and prednisolone. The authors present seven cases of biopsy-proven acute rejection after kidney transplantation refractory to conventional rejection the...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000147197.24050.61
更新日期:2005-01-15 00:00:00
abstract::Acute rejection is a frequent cause of early graft dysfunction in renal transplantation, and serum creatinine is the most important measure of clinical response. However, there is little information on serum creatinine during rejection episodes. In this study, the determinants that might influence the clinical respons...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199507150-00006
更新日期:1995-07-15 00:00:00
abstract:BACKGROUND:The aim of the study was to assess the incidence of cytomegalovirus (CMV) infection and disease in adult liver transplant recipients, using routine preemptive therapy guided by the pp65 antigenemia test. METHODS:Antigenemia was monitored weekly after liver transplantation (OLTX) for the first 3 months, and ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199910270-00011
更新日期:1999-10-27 00:00:00
abstract::Allograft rejection results from a complex process involving both the innate and acquired immune systems. The innate immune system predominates in the early phase of the allogeneic response, during which chemokines and cell adhesion play essential roles, not only for leukocyte migration into the graft but also for fac...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/00007890-200205150-00001
更新日期:2002-05-15 00:00:00
abstract:BACKGROUND:Primary hyperoxaluria type-1 (PH1) is an autosomal recessive disease characterized by excessive oxalate production by hepatocytes caused by the deficiency of peroxisomal alanine-glyoxylate aminotransferase (AGT) activity. Persistent hyperoxaluria causes nephrocalcinosis and urolithiasis, leading to renal fai...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31816de49e
更新日期:2008-05-15 00:00:00
abstract::Organ donation after circulatory death (DCD) has been endorsed by the World Health Organization and is practiced worldwide. This overview examines current DCD practices, identifies problems and challenges, and suggests clinical strategies for possible improvement. Although there is uniform agreement on DCD donor candi...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/01.TP.0000437178.48174.db
更新日期:2014-02-15 00:00:00