Abstract:
:The lack of a convenient diagnostic method for onchocerciasis has motivated attempts to develop better detection techniques. Preliminary results indicate that a dot blot assay of the total immunoglobulin G (IgG) response to an easily available and simply prepared Tris buffer soluble fraction (TSF) of whole female worms may be applicable in the field as a first screening method. The specificity of the assay is improved by using a partially purified fraction of TSF of which the dominant component is a 23 kDa protein antigen, PakF. The IgG response to PakF was 100% sensitive and at least as specific as the IgG4 response to TSF. Sera from 189 individuals including onchocerciasis patients and apparently uninfected control subjects from an endemic area and from an urban area in Ghana were screened. The specificity of the assay with urban and endemic control sera was 93% and 68% respectively. The dot blot assay has advantages over the existing skin snip and enzyme-linked immunosorbent assay techniques in terms of simplicity, cost, consumption of antigen, risk of spreading other infections, and patient comfort.
journal_name
Trans R Soc Trop Med Hygauthors
Lavebratt C,Dalhammar G,Adamafio NA,Nykänen-Dejerud U,Mingarini K,Ingemarsson K,Opoku N,Akuffo HOdoi
10.1016/0035-9203(94)90090-6subject
Has Abstractpub_date
1994-05-01 00:00:00pages
303-6issue
3eissn
0035-9203issn
1878-3503journal_volume
88pub_type
杂志文章abstract::The severity and consequences of human Trichuris infection vary widely. This may reflect differences in immune response. T. muris is a natural parasite of mice which provides a close, well defined, easily manipulated model for otherwise uninvestigatable aspects of this response. Normal mice reject the parasite in 3 we...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/0035-9203(94)90074-4
更新日期:1994-05-01 00:00:00
abstract:BACKGROUND:Hump-nosed pit vipers (Hypnale hypnale) are highly venomous snakes found in Sri Lanka and the Western Ghats region of India. They are the most common cause of venomous snakebites in Sri Lanka, mainly causing local envenoming leading to pain, swelling and necrosis. Systemic envenoming causing acute kidney inj...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1093/trstmh/try120
更新日期:2019-03-01 00:00:00
abstract::The liver fluke Opisthorchis felineus is a member of the triad of epidemiologically important liver fluke species belonging to the family Opisthorchiidae and the major agent causing opisthorchiasis over a vast territory, covering Russia, Kazakhstan and several European countries. The similarity between the diseases ca...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章,评审
doi:10.1093/trstmh/trv085
更新日期:2016-01-01 00:00:00
abstract::Promotion of breastfeeding is a key component of infant health policies globally. However, maternal HIV infection is a possible contraindication for breastfeeding since it can result in mother-to-child transmission. Research into infant-feeding strategies which will promote the health of all infants but also protect a...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章,评审
doi:10.1016/s0035-9203(03)90010-1
更新日期:2003-01-01 00:00:00
abstract::In Amazonian Brazil most human leishmaniasis is due to Leishmania braziliensis s.l. and is acquired during the clearing of primary climax forest. One of the largest deforestation projects has taken place on the JARI property where plantations of exotic tree species are grown for paper pulp. The ability of the regional...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/0035-9203(83)90288-2
更新日期:1983-01-01 00:00:00
abstract:BACKGROUND:Schistosoma haematobium infection is a major public health problem in most of Africa and the Middle East and praziquantel remains the only drug used for schistosomiasis control, therefore emergence of drug resistance is unavoidable. The antimalarial artemisinin-naphthoquine phosphate combination (co-ArNp) wa...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1093/trstmh/try144
更新日期:2019-06-01 00:00:00
abstract::Inadequate evaluation of vaccine coverage after mass vaccination campaigns, such as used in national measles control programmes, can lead to inappropriate public health responses. Overestimation of vaccination coverage may leave populations at risk, whilst underestimation can lead to unnecessary catch-up campaigns. Th...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/j.trstmh.2007.10.015
更新日期:2008-03-01 00:00:00
abstract::This study was conducted to reassess the risk factors for giardiasis in communities of the Orang Asli (indigenous people) in Pahang, Malaysia. Stool samples were collected from 321 individuals (2-76 years old; 160 males, 161 females). Data were collected via laboratory analysis of faecal samples and a pre-tested stand...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章,多中心研究
doi:10.1016/j.trstmh.2008.02.004
更新日期:2008-05-01 00:00:00
abstract::The bacterial flora of the upper intestine has been examined in symptomatic expatriate adults with mild tropical malabsorption, without steatorrhoea, persisting for many months after return to a western environment. Seven of the 11 patients had enterobacteria in luminal fluid or mucosal samples in numbers ranging from...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/0035-9203(80)90194-7
更新日期:1980-01-01 00:00:00
abstract::Study of the effects of malaria chemoprophylaxis given during pregnancy on birthweight and investigation of the influence of birthweight on child survival suggest that, in a rural area of The Gambia, chemoprophylaxis given during pregnancy might reduce infant mortality by about one-fifth in the children of primigravid...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0035-9203(92)90078-q
更新日期:1992-09-01 00:00:00
abstract::In the global effort to eliminate lymphatic filariasis, annual mass treatments are conducted with diethylcarbamazine (DEC) or ivermectin, combined with albendazole. The success of this strategy depends on achieving high levels of drug coverage, which reduces the number of persons with circulating microfilariae so that...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/s0035-9203(03)80006-8
更新日期:2003-09-01 00:00:00
abstract:BACKGROUND:Manual white blood cell (WBC) differential counts as a predictor for neonatal sepsis development in a low-resource setting have not been thoroughly evaluated. We hypothesized that manual differentiation (specifically immature:total [I:T] neutrophil ratios) would be feasible and useful as an adjunct to predic...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1093/trstmh/traa023
更新日期:2020-08-01 00:00:00
abstract::Difluoromethylornithine (DFMO), a specific, irreversible inhibitor of polyamine biosynthesis shown to be curative in animal models inoculated with various Trypanosoma spp., was evaluated in the Southern Sudan in a preliminary open clinical field trial in patients infected with Trypanosoma brucei gambiense. 20 patients...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 临床试验,杂志文章
doi:10.1016/0035-9203(85)90195-6
更新日期:1985-01-01 00:00:00
abstract::A sensitive and specific polymerase chain reaction (PCR) based on a highly repeated deoxyribonucleic acid (DNA) sequence (188 bp; SspI repeat) was tested for the detection of Wuchereria bancrofti DNA in blood and urine samples collected during the day from individuals in Coque, Recife, Brazil, an endemic area for W. b...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/s0035-9203(98)91016-1
更新日期:1998-05-01 00:00:00
abstract::112 Leishmania isolates, obtained in French Guiana from human lesions, phlebotomine sandflies and wild mammals, were characterized by isoenzyme electrophoresis. Leishmania braziliensis guyanensis and L. mexicana amazonensis were found parasitizing different natural hosts. L.b. guyanensis was the dominant species (103 ...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/0035-9203(89)90373-8
更新日期:1989-09-01 00:00:00
abstract::The involvement of the lung in 13 cases of human visceral leishmaniasis was studied. Interstitial pneumonitis with mononuclear cells was found in 76.8% of the cases; 53.8% also had foci of septal fibrosis. Leishmania were seen within macrophages in 3 cases only. However, all 10 interstitial pneumonitis cases showed PA...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/0035-9203(89)90712-8
更新日期:1989-01-01 00:00:00
abstract::The response of Plasmodium falciparum to chloroquine in Zambia was determined by the in vivo and in vitro microtechniques. A total dose of 25mg chloroquine base/kg body weight (over 3 d) was administered to 60 children at Lubwe and 42 at Kalene. The in vivo test was successfully completed on 55 cases at Lubwe and 39 a...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/0035-9203(88)90406-3
更新日期:1988-01-01 00:00:00
abstract:BACKGROUND:The objective of this study was to assess the impact of sputum quality and quantity on smear and culture positivity for Mycobacterium tuberculosis. METHODS:A total of 14 708 sputum specimens, collected from respiratory symptomatics in a TB prevalence survey, were evaluated for gross appearance and volume of...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1093/trstmh/trt100
更新日期:2014-01-01 00:00:00
abstract::Primary peritonitis that tends to occur especially in children, and cirrhotic spontaneous bacterial peritonitis are well recognized clinical entities. At the University College Hospital, Ibadan, cases of intra-abdominal abscess that do not fit the recognized criteria for primary peritonitis have been seen. These patie...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/0035-9203(82)90022-0
更新日期:1982-01-01 00:00:00
abstract::A study was undertaken to assess the role of cockroaches as potential carriers of human intestinal parasites in Addis Ababa and Ziway, Ethiopia. A total of 6480 cockroaches were trapped from the two localities from October 2006 to March 2007. All the cockroaches trapped in Addis Ababa (n=2240) and almost 50% (2100/424...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/j.trstmh.2008.05.009
更新日期:2008-11-01 00:00:00
abstract::There is a lack of information on the disease burden due to Taenia solium taeniasis and its associated risk factors in pig farming communities throughout the world. The present study was conducted in a rural pig farming community of north India to estimate the prevalence of T. solium taeniasis and associated factors. ...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/j.trstmh.2007.04.019
更新日期:2007-12-01 00:00:00
abstract::Tuberculosis (TB) of the breast is a very rare pathology. The clinical presentations of this disease are manifold, often mimicking carcinoma of the breast. We conducted a retrospective analysis of 20 women with TB of the breast receiving care at the mastology clinic at Getúlio Vargas Hospital, PI, Brazil, between 1994...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章,评审
doi:10.1016/j.trstmh.2009.02.005
更新日期:2009-06-01 00:00:00
abstract::The activities of serum and urinary alpha-esterases were studied in 49 bilharzial bladder cancer patients, 92 bilharzial patients with other, different, urologic diseases and 22 normal healthy controls. Among the group studied, the bilharzial bladder cancer patients showed the highest level of urinary-alpha-esterases ...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/0035-9203(78)90176-1
更新日期:1978-01-01 00:00:00
abstract::Tinea imbricata, also known as 'Tokelau', is an uncommon superficial mycosis caused by the anthropophilic dermatophyte Trichophyton concentricum. Cutaneous lesions appear characteristically as scaly and concentric rings that may cover all parts of the body. Often acquired in childhood, tinea imbricata is a chronic dis...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/j.trstmh.2008.01.002
更新日期:2008-04-01 00:00:00
abstract::A survey of enteric pathogens in 143 children with diarrhoea, 116 age-matched controls and 524 asymptomatic people of all ages in a commercial farming area was carried out over a period of one year. Giardia lamblia was detected in 34% of the cases, 29% of the controls and 23% of asymptomatic people of all ages. Variou...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/0035-9203(87)90403-2
更新日期:1987-01-01 00:00:00
abstract::We conducted experiments in vitro to assess the effect of artemether in combination with haemin on adult Schistosoma japonicum, S. mansoni and S. haematobium. When schistosomes were maintained in a medium containing artemether at concentrations of 20 micrograms/mL or less for 72 h, no apparent effect on the schistosom...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/s0035-9203(01)90336-0
更新日期:2001-01-01 00:00:00
abstract::In Malawi, where high levels of chloroquine resistance were shown using a modified 7-day in vivo test, amodiaquine and pyrimethamine-sulfadoxine were evaluated as alternative initial therapies for Plasmodium falciparum infections in children under 5 years old. Therapy success rates, judged by parasite clearance by day...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/0035-9203(87)90005-8
更新日期:1987-01-01 00:00:00
abstract::Data on malaria transmission intensity and prevalences of asexual parasites and of gametocytes were obtained in an urban district of Yaoundé, Cameroon. The transmission level from mosquito to human was determined by indoor night capture of mosquitoes on human volunteers, revealing a calculated entomological inoculatio...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/s0035-9203(03)90059-9
更新日期:2003-07-01 00:00:00
abstract::By microinjection of cryopreserved microfilariae (mf) into nulliparous flies, a comparison of the lengths of the infective larvae (L3) of Onchocerca volvulus and O. ochengi from the head of Simulium damnosum s.l. (presumed S. sirbanum) has been made. The suitability of S. sirbanum as a host was similar for both Onchoc...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/0035-9203(92)90443-g
更新日期:1992-01-01 00:00:00
abstract::Indoor-resting anopheline mosquitoes were collected from Obuasi, Ghana, and were identified morphologically and by PCR as Anopheles funestus Giles. Wild-caught females were induced to lay eggs. Samples of F1 progeny from each family were divided into cohorts and were either exposed to DDT and permethrin or were stored...
journal_title:Transactions of the Royal Society of Tropical Medicine and Hygiene
pub_type: 杂志文章
doi:10.1016/j.trstmh.2008.02.022
更新日期:2008-06-01 00:00:00