Abstract:
RATIONALE:Pulmonary hypertensive remodeling is characterized by excessive proliferation, migration, and proinflammatory activation of adventitial fibroblasts. In culture, fibroblasts maintain a similar activated phenotype. The mechanisms responsible for generation/maintenance of this phenotype remain unknown. OBJECTIVE:We hypothesized that aberrant expression of microRNA-124 (miR-124) regulates this activated fibroblast phenotype and sought to determine the signaling pathways through which miR-124 exerts effects. METHODS AND RESULTS:We detected significant decreases in miR-124 expression in fibroblasts isolated from calves and humans with severe pulmonary hypertension. Overexpression of miR-124 by mimic transfection significantly attenuated proliferation, migration, and monocyte chemotactic protein-1 expression of hypertensive fibroblasts, whereas anti-miR-124 treatment of control fibroblasts resulted in their increased proliferation, migration, and monocyte chemotactic protein-1 expression. Furthermore, the alternative splicing factor, polypyrimidine tract-binding protein 1, was shown to be a direct target of miR-124 and to be upregulated both in vivo and in vitro in bovine and human pulmonary hypertensive fibroblasts. The effects of miR-124 on fibroblast proliferation were mediated via direct binding to the 3' untranslated region of polypyrimidine tract-binding protein 1 and subsequent regulation of Notch1/phosphatase and tensin homolog/FOXO3/p21Cip1 and p27Kip1 signaling. We showed that miR-124 directly regulates monocyte chemotactic protein-1 expression in pulmonary hypertension/idiopathic pulmonary arterial hypertension fibroblasts. Furthermore, we demonstrated that miR-124 expression is suppressed by histone deacetylases and that treatment of hypertensive fibroblasts with histone deacetylase inhibitors increased miR-124 expression and decreased proliferation and monocyte chemotactic protein-1 production. CONCLUSIONS:Stable decreases in miR-124 expression contribute to an epigenetically reprogrammed, highly proliferative, migratory, and inflammatory phenotype of hypertensive pulmonary adventitial fibroblasts. Thus, therapies directed at restoring miR-124 function, including histone deacetylase inhibitors, should be investigated.
journal_name
Circ Resjournal_title
Circulation researchauthors
Wang D,Zhang H,Li M,Frid MG,Flockton AR,McKeon BA,Yeager ME,Fini MA,Morrell NW,Pullamsetti SS,Velegala S,Seeger W,McKinsey TA,Sucharov CC,Stenmark KRdoi
10.1161/CIRCRESAHA.114.301633subject
Has Abstractpub_date
2014-01-03 00:00:00pages
67-78issue
1eissn
0009-7330issn
1524-4571pii
CIRCRESAHA.114.301633journal_volume
114pub_type
杂志文章abstract::We determined the effects of indomethacin and meclofenamate, two inhibitors of prostaglandin synthesis, on renal vascular resistance and on renal responses to nerve stimulation, pressor and depressor hormones in the in situ feline kidney under conditions of controlled blood flow. Both inhibitors produced a gradual ris...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.40.4.348
更新日期:1977-04-01 00:00:00
abstract::Nuclear factor kappa B (NF-kappaB) participates in hypertension-induced vascular and target-organ damage. We tested whether or not endothelial cell-specific NF-kappaB suppression would be ameliorative. We generated Cre/lox transgenic mice with endothelial cell-restricted NF-kappaB super-repressor IkappaBalphaDeltaN (T...
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doi:10.1161/01.res.81.4.493
更新日期:1997-10-01 00:00:00
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doi:10.1161/01.res.69.1.26
更新日期:1991-07-01 00:00:00
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doi:10.1161/01.res.52.1.57
更新日期:1983-01-01 00:00:00
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journal_title:Circulation research
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doi:10.1161/01.res.36.1.8
更新日期:1975-01-01 00:00:00
abstract::Patients with diabetes mellitus have >2× the risk for developing heart failure (HF; HF with reduced ejection fraction and HF with preserved ejection fraction). Cardiovascular outcomes, hospitalization, and prognosis are worse for patients with diabetes mellitus relative to those without. Beyond the structural and func...
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doi:10.1161/01.res.46.4.470
更新日期:1980-04-01 00:00:00
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journal_title:Circulation research
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doi:10.1161/01.res.56.4.606
更新日期:1985-04-01 00:00:00
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doi:10.1161/01.res.78.2.180
更新日期:1996-02-01 00:00:00
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doi:10.1161/01.res.42.1.35
更新日期:1978-01-01 00:00:00
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doi:10.1161/01.res.85.9.820
更新日期:1999-10-29 00:00:00
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更新日期:2002-12-13 00:00:00
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更新日期:2017-06-09 00:00:00
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journal_title:Circulation research
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更新日期:1989-05-01 00:00:00
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更新日期:1982-12-01 00:00:00
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更新日期:2000-04-14 00:00:00
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更新日期:1998-01-09 00:00:00
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pub_type: 杂志文章,meta分析,评审
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更新日期:2018-04-13 00:00:00
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更新日期:1986-01-01 00:00:00
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doi:10.1161/01.res.75.2.268
更新日期:1994-08-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2006-10-27 00:00:00
abstract::The hallmark of most cardiac diseases is the progressive loss of cardiomyocytes. In the perinatal period, cardiomyocytes still proliferate, and the heart shows the capacity to regenerate upon injury. In the adult heart, however, the actual rate of cardiomyocyte renewal is too low to efficiently counteract substantial ...
journal_title:Circulation research
pub_type: 杂志文章,评审
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更新日期:2020-02-14 00:00:00
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pub_type: 杂志文章
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更新日期:2000-11-24 00:00:00
abstract:RATIONALE:Previous studies on the relationship between diabetes and arterial stiffness were mostly cross-sectional. A few longitudinal studies focused on one single direction. Whether the association between arterial stiffness and diabetes is bidirectional remains unclear to date. OBJECTIVE:To explore the temporal rel...
journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2020-12-04 00:00:00
abstract::The epicardium, the tissue layer covering the cardiac muscle (myocardium), develops from the proepicardium, a mass of coelomic progenitors located at the venous pole of the embryonic heart. Proepicardium cells attach to and spread over the myocardium to form the primitive epicardial epithelium. The epicardium subseque...
journal_title:Circulation research
pub_type: 杂志文章,评审
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更新日期:2011-12-09 00:00:00