Effects of epidermal growth factor and Clostridium difficile toxin B in a model of mucosal injury.

Abstract:

:Numerous factors have been advocated as being paramount to the development of necrotizing enterocolitis (NEC) including hypoxia, abnormal bacterial flora, and by products of enteral feedings. In an effort to better understand mechanisms involved at the level of the intestinal mucosal barrier the authors have chosen the CACO-2 cell line to model the neonatal intestinal epithelium. By growing CACO-2 cells in transwell inserts, the authors have investigated the ability of Clostridium difficile toxin B, epidermal growth factor (EGF), and a model of mechanical injury to alter transepithelial resistance of CACO-2 monolayers. The findings show that toxin B diminishes resistance in this setting, and EGF can alter that resistance drop.

journal_name

J Pediatr Surg

authors

Lawrence JP,Brevetti L,Obiso RJ,Wilkins TD,Kimura K,Soper R

doi

10.1016/s0022-3468(97)90598-4

subject

Has Abstract

pub_date

1997-03-01 00:00:00

pages

430-3

issue

3

eissn

0022-3468

issn

1531-5037

pii

S0022-3468(97)90598-4

journal_volume

32

pub_type

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