The nemaline myopathy-causing E117K mutation in β-tropomyosin reduces thin filament activation.

abstract：:The oxidizing power of the thiyl radical (GS*) produced on oxidation of glutathione (GSH) was determined as the mid-point electrode potential (reduction potential) of the one-electron couple E(m)(GS*,H+/GSH) in water, as a function of pH over the physiological range. The method involved measuring the equilibrium const...

journal_title：Archives of biochemistry and biophysics

authors： Madej E,Wardman P

更新日期：2007-06-01 00:00:00

abstract：:Previously, we reported that emptying of intracellular Ca(2+) pools with endoplasmatic Ca(2+)-ATP-ase inhibitor thapsigargin leads to the Na(+) influx in human lymphocytes (M. Tepel et al., 1994, J. Biol. Chem. 269, 26239-26242). In the present study we examined the mechanism underlying the thapsigargin-induced Na(+) ...

journal_title：Archives of biochemistry and biophysics

authors： Nofer JR,Junker R,Walter M,Seedorf U,Assmann G,Zidek W,Tepel M

更新日期：2000-02-15 00:00:00

abstract：:Human plasma glutathione peroxidase was purified to homogeneity and partially sequenced. Overlapping peptide fragments from three endopeptidase digests permitted the determination of one sequence of 32 contiguous amino acids and one sequence of 23 contiguous amino acids. Five additional unique peptide sequences withou...

journal_title：Archives of biochemistry and biophysics

authors： Esworthy RS,Chu FF,Paxton RJ,Akman S,Doroshow JH

更新日期：1991-05-01 00:00:00

abstract：:Alcohol oxidase (AO) is a peroxisomal, homo-octameric flavoenzyme, which catalyzes methanol oxidation in methylotrophic yeast. Here, we report on the generation of soluble, FAD-lacking AO monomers. Using steady-state fluorescence, fluorescence correlation spectroscopy, circular dichroism and static light scattering ap...

journal_title：Archives of biochemistry and biophysics

authors： Visser NV,Wang D,Stanley WA,Groves MR,Wilmanns M,Veenhuis M,van der Klei IJ

更新日期：2007-03-15 00:00:00

abstract：:Soybean Bowman-Birk protease inhibitor (BBI) is an inhibitor of serine proteases with two functional inhibitory domains of different specificities: one is specific for chymotrypsin-like proteases, the other for trypsin-like proteases. Chymase and tryptase are serine proteases which are stored in mast cell granules and...

journal_title：Archives of biochemistry and biophysics

authors： Ware JH,Wan XS,Rubin H,Schechter NM,Kennedy AR

更新日期：1997-08-01 00:00:00

abstract：:A lens epithelial cell line established from a transgenic mouse synthesizes high levels of the enzyme aldose reductase which converts sugars to polyols. This enzyme has been implicated in the formation of sugar cataracts in animals and with diabetic complications in man. The mouse aldose reductase has been characteriz...

journal_title：Archives of biochemistry and biophysics

authors： Russell P,Yamada T,Merola LO

更新日期：1990-01-01 00:00:00

abstract：:Liver mitochondria were incubated through extended State 3-State 4 transitions (B. Chance and G. N. Williams (1955) J. Biol. Chem. 217, 409-423) in the presence of high concentrations of adenine nucleotides and in presence and absence of a protonophore. In the terminal phase of these transitions (the region of respira...

journal_title：Archives of biochemistry and biophysics

authors： Davis EJ,Davis-van Thienen WI

更新日期：1989-12-01 00:00:00

abstract：:Cellular distribution in rat livers of the mannan-binding protein, a liver lectin specific for mannose and N-acetylglucosamine was investigated. Estimation of mannan binding activity of isolated cells demonstrated that more than 99% of the mannan binding activity recovered from whole livers was derived from hepatocyte...

journal_title：Archives of biochemistry and biophysics

authors： Mori K,Kawasaki T,Yamashina I

更新日期：1983-04-15 00:00:00

abstract：:A ganglioside GM1-specific alpha 1-->2fucosyltransferase is induced during the early stages of chemical carcinogenesis with N-2-acetylaminofluorene (AAF) in rat liver hepatocytes. The induction of this enzyme gives rise to the expression of a fucose-containing ganglioside with the same determinant structure as blood g...

journal_title：Archives of biochemistry and biophysics

authors： Sherwood AL,Holmes EH

更新日期：1998-07-15 00:00:00

abstract：:The binding of Toxin A isolated from Clostridium difficile to rabbit erythrocyte glycolipids has been studied. Total lipid extracts from rabbit erythrocytes were subjected to thin-layer chromatography and toxin-binding glycolipids detected by using 125I-labeled Toxin A in a direct binding overlay technique. Two major ...

journal_title：Archives of biochemistry and biophysics

authors： Clark GF,Krivan HC,Wilkins TD,Smith DF

更新日期：1987-08-15 00:00:00

abstract：:Escherichia coli pyruvate oxidase (POXEC) requires FAD both for the oxidative decarboxylation of pyruvate to acetate and CO2 and for the formation of acetoin from pyruvate and acetaldehyde. Prior work has shown that the catalytic activity (kcat/Km) for POXEC in the oxidative reaction is stimulated approximately 450-fo...

journal_title：Archives of biochemistry and biophysics

authors： Bertagnolli BL,Hager LP

更新日期：1993-01-01 00:00:00

abstract：:The cDNA clone lambda ML-1 encoding one of the extracellular lignin peroxidases from the white rot fungus, Phanerochaete chrysosporium, was heterologously expressed in an active form using a recombinant baculovirus system. The glycosylated extracellular form of the recombinant protein contained the ferriprotoporphyrin...

journal_title：Archives of biochemistry and biophysics

authors： Johnson TM,Li JK

更新日期：1991-12-01 00:00:00

abstract：:We have isolated and characterized a human liver glutathione S-transferase Ha1 subunit gene. The gene spans approximately 12 kilobases and comprises seven exons separated by six introns. The transcription initiation site has been determined by primer extension analysis. A TATA box is located 26 nucleotides upstream fr...

journal_title：Archives of biochemistry and biophysics

authors： Rozen F,Nguyen T,Pickett CB

更新日期：1992-02-01 00:00:00

abstract：:Enzymes subjected to freeze-thawing are known to be protected by polymers that are preferentially excluded from the hydrated surface of proteins [reviewed in Carpenter et al. (1994) ACS Symp. Ser. 567, 134-147]. Preferentially excluded solutes are also known to stabilize quaternary structure, which enhances the thermo...

journal_title：Archives of biochemistry and biophysics

authors： Anchordoquy TJ,Carpenter JF

更新日期：1996-08-15 00:00:00

abstract：:The protein kinase C (PKC) family of enzymes plays a key role in the regulation of cellular events, including cell proliferation and differentiation. Work from our laboratory has shown that the effects of dietary fat and fiber on colonic cell proliferation were positively correlated with membrane/cytosol PKC activity ...

journal_title：Archives of biochemistry and biophysics

authors： Davidson LA,Jiang YH,Derr JN,Aukema HM,Lupton JR,Chapkin RS

更新日期：1994-08-01 00:00:00

abstract：:Human malic enzyme was studied by steady state kinetics, deuterium isotope effects, and 13C isotope effects with both the physiological dinucleotide cofactor and several alternate cofactors. The log V vs pH profile with NAD revealed two pK(a) values too close to be separately determined, but with an average value of 7...

journal_title：Archives of biochemistry and biophysics

authors： Rishavy MA,Yang Z,Tong L,Cleland WW

更新日期：2001-12-01 00:00:00

abstract：:Dipeptidyl-peptidase IV (EC 3.4.14.5) also known as CD26 is a membrane-bound serine peptidase which cleaves N-terminal dipeptides from a peptide chain containing a proline residue in the penultimate position. The enzyme is believed to play an important role in neuropeptide metabolism and T-cell activation. A series of...

journal_title：Archives of biochemistry and biophysics

authors： Li J,Wilk E,Wilk S

更新日期：1995-10-20 00:00:00

abstract：:Because angiogenesis underlies the pathogenesis of numerous conditions (cancer, psoriasis, macular degeneration), there is a pressing need for continued investigations into angiogenic signaling and potential drug targets. Antiangiogenic agents can be classified as either direct or indirect. Direct antiangiogenics act ...

journal_title：Archives of biochemistry and biophysics

authors： Berrios RL,Arbiser JL

更新日期：2011-04-15 00:00:00

abstract：:Ischemia/reperfusion injury (IRI) initiates from oxidative stress caused by lack of blood supply and subsequent reperfusion. It is often associated with sterile inflammation, cell death and microvascular dysfunction, which ultimately results in myocardial, cerebral and hepatic IRIs. Reportedly, deregulation of Nrf2 pa...

journal_title：Archives of biochemistry and biophysics

authors： Padmavathi G,Ramkumar KM

更新日期：2021-02-15 00:00:00

abstract：:A numerical method was applied to a system of differential rate equations describing the monomer-dimer-inhibitor (M-D-I) interaction involving human immunodeficiency virus type 1 protease and a peptidomimetic, competitive inhibitor. Two pairs of progress curves were obtained, one involving the M-D interaction and the ...

journal_title：Archives of biochemistry and biophysics

authors： Morelock MM,Graham ET,Erdman D,Pargellis CA

更新日期：1996-04-15 00:00:00

abstract：:Osmolytes are small organic molecules accumulated by organisms under stress conditions to protect macromolecular structure and function. In the present study, we have investigated the effect of several binary osmolyte mixtures on the protein folding/stability and function of RNase-A. For this, we have measured ΔGD(o) ...

journal_title：Archives of biochemistry and biophysics

authors： Warepam M,Singh LR

更新日期：2015-05-01 00:00:00

abstract：:The reaction mechanism of a 1,4-benzoquinone reductase from the wood-rotting basidiomycete Phanerochaete chrysosporium was investigated. The native, oxidized, FMN-containing enzyme was reduced quantitatively by NADH and the resulting reduced enzyme was reoxidized in the presence of one equivalent of 2,6-di-methoxy-1,4...

journal_title：Archives of biochemistry and biophysics

authors： Brock BJ,Gold MH

更新日期：1996-07-01 00:00:00

abstract：:We have analyzed the effect of chlorpromazine (CPZ) on pure actin. We have found that CPZ quenches Trp-79 and Trp-86 fluorescence and, in agreement with an earlier report on conventional actin, inhibits actin polymerization, lowering the extent of polymerization. Moreover, novel polymerization data are presented indic...

journal_title：Archives of biochemistry and biophysics

authors： Milzani A,DalleDonne I

更新日期：1999-09-01 00:00:00

abstract：:Peroxynitrite, a biological oxidant formed from the reaction of nitric oxide with the superoxide radical, is associated with many pathologies, including neurodegenerative diseases, such as multiple sclerosis (MS). Gout (hyperuricemic) and MS are almost mutually exclusive, and uric acid has therapeutic effects in mice ...

journal_title：Archives of biochemistry and biophysics

authors： Squadrito GL,Cueto R,Splenser AE,Valavanidis A,Zhang H,Uppu RM,Pryor WA

更新日期：2000-04-15 00:00:00

abstract：:Chloroplast tRNA(Glu) is a bifunctional molecule involved in both the early steps of chlorophyll synthesis and chloroplast protein biosynthesis. Recently the enzymes involved in these processes have been characterized from the green alga Chlamydomonas reinhardtii. In order to investigate whether transcription of the g...

journal_title：Archives of biochemistry and biophysics

authors： Jahn D

更新日期：1992-11-01 00:00:00

abstract：:The reaction of the Cu,Co derivative of bovine Cu,Zn superoxide dismutase with phenylglyoxal or butanedione, which are known to inactivate the enzyme by selectively binding to Arg 141, has been studied by 1H NMR. Several 1H NMR lines of the copper-liganding histidine residues were perturbed, reproducing an effect so f...

journal_title：Archives of biochemistry and biophysics

authors： Paci M,Desideri A,Sette M,Rotilio G

更新日期：1991-04-01 00:00:00

abstract：:Sulfotyrosyl peptides corresponding to the known high-affinity substrate phosphotyrosyl peptide sequences in casein and the autophosphorylation sites of insulin receptor and EGF receptor were investigated as inhibitors of protein tyrosine phosphatases PTP1B and CD45. These peptides inhibit both PTP1B and CD45 in the m...

journal_title：Archives of biochemistry and biophysics

authors： Desmarais S,Jia Z,Ramachandran C

更新日期：1998-06-15 00:00:00

abstract：:Liver mitochondria provided with an oxidizable substrate, ATP, oxygen, and an ADP-generating system (soluble F1-ATPase) were used to reevaluate the rate-controlling step(s) intrinsic to all of the processes of mitochondrial oxidative phosphorylation. The quantity termed "control strength" (C), previously defined as th...

journal_title：Archives of biochemistry and biophysics

authors： Davis EJ,Davis-Van Thienen WI

更新日期：1984-09-01 00:00:00

abstract：:Glucosidase I, the enzyme catalyzing the first step of N-linked oligosaccharide processing, has been purified from calf liver crude membranes [H. Hettkamp, G. Legler, and E. Bause, (1984) Eur. J. Biochem. 142, 85-90]. Binding experiments with concanavalin A-Sepharose suggest that glucosidase I is a glycoprotein with h...

journal_title：Archives of biochemistry and biophysics

authors： Schweden J,Borgmann C,Legler G,Bause E

更新日期：1986-07-01 00:00:00

abstract：:Human tumor necrosis factor (TNF), first isolated by our group as an anticancer agent, has been now shown to be a primary mediator of inflammation. Till today 19 different members of the TNF superfamily which interact with 29 different receptors, have been identified. Most members of this family exhibit pro-inflammato...

journal_title：Archives of biochemistry and biophysics

authors： Gupta SC,Tyagi AK,Deshmukh-Taskar P,Hinojosa M,Prasad S,Aggarwal BB

更新日期：2014-10-01 00:00:00