Reliability of cytology to detect chlamydial infection in asymptomatic women.

Abstract:

:Chlamydia trachomatis is a frequent sexually transmitted disease. The diagnosis of C. trachomatis infection by cytology is controversial. We compared the ability of Papanicolaou (Pap) smears to detect C. trachomatis infection with antigen detection (enzyme immunoassay; EIA) and polymerase chain reaction (PCR). One hundred sixty-seven women attending a therapeutic abortion clinic were enrolled in the study. Endocervical samples were first collected for EIA and PCR, and then Pap smears were prepared for cytologic evaluation. Eight patients were excluded from the study due to the lack of an endocervical component. The criteria established by Gupta and associates (Diagn Cytopathol 1988;4:224-229; Acta Cytol 1979;23:315-320) were used in this study to assess the specificity and sensitivity of the Pap smear in recognizing C. trachomatis infection. After EIA testing, the remaining sample was subjected to phenol-chloroform extraction to purify the DNA and then tested by PCR. Positive PCR samples were subjected to repeat phenol-chloroform and retested to confirm the positive result. Using a confirmed PCR or a blocked EIA as the extended gold standard, the incidence of C. trachomatis infection was 9.4%. Fifteen of the 159 cases reviewed were positive by extended gold standard. Thirteen (86.7%) of those 15 cases were interpreted as negative by cytology (false-negatives), and two (13.3%) cases were positive. Of the remaining 144 cases, 14 cases (9.7%) were interpreted as positive by cytology (false-positives) but were not confirmed by the extended gold standard. Ten (66.7%) of the 15 cases confirmed by the extended gold standard were interpreted as negative by EIA (false-negatives), and five (33.3%) were positive. There were no false-positives by EIA. In this study, the sensitivity and the specificity for cytology were 13.3% and 90.3%, respectively. The positive predictive value was 12.5%, and the negative predictive value for cytology was 90.9%. The sensitivity and the specificity for EIA were 33.3% and 100%, respectively. The positive predictive value was 100%, and the negative predictive value for EIA was 93.5%. Both EIA and cytology are insensitive methods compared with PCR. Based on these data, cytology should not be used to diagnose C. trachomatis infection in an asymptomatic female population with a moderate risk of C. trachomatis infection.

journal_name

Diagn Cytopathol

journal_title

Diagnostic cytopathology

authors

Vinette-Leduc D,Yazdi HM,Jessamine P,Peeling RW

doi

10.1002/(sici)1097-0339(199710)17:4<258::aid-dc4>3

subject

Has Abstract

pub_date

1997-10-01 00:00:00

pages

258-61

issue

4

eissn

8755-1039

issn

1097-0339

pii

10.1002/(SICI)1097-0339(199710)17:4<258::AID-DC4>3

journal_volume

17

pub_type

杂志文章
  • Cytopathologic characteristics of SMARCB1 (INI-1) deficient sinonasal carcinoma: A potential diagnostic pitfall.

    abstract::Tumors of the head and neck are extremely diverse and a subset are poorly differentiated and difficult to classify. Recently, a new entity has been described with rhabdoid and/or plasmacytoid cytologic features and a characteristic genetic signature-inactivation of the SMARCB1 (INI-1) tumor suppressor gene. To date, o...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23503

    authors: Allison DB,Bishop JA,Ali SZ

    更新日期:2016-08-01 00:00:00

  • Chest wall abscess: an unusual presentation of Hodgkin's lymphoma.

    abstract::A chest wall abscess is a very rare presentation of extranodal Hodgkin's lymphoma (HL); only one case has been reported to date. Here, we describe a case of a 38-yr-old man with HL whose initial presentation was a chest wall abscess. The diagnosis of HL was suggested by cytological examination of the purulent discharg...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20220

    authors: Khalbuss WE,Grigorian S,Mignone J,D'Agostino HJ

    更新日期:2005-07-01 00:00:00

  • Localization of p53 and proliferating cell nuclear antigen in fine-needle aspirates of benign and primary malignant tumors of the human breast: an immunocytochemical study using supersensitive monoclonal antibodies and the biotin-streptavidin-amplified me

    abstract::p53 and proliferating cell nuclear antigen (PCNA) status was determined in fine-needle aspirates (FNAs) and methacarn-fixed paraffin-embedded tissue sections of six fibroadenomas and 50 primary breast carcinomas using supersensitive monoclonal antibodies and the biotin-streptavidin-amplified method. Nuclear accumulati...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0339(199611)15:4<277::AID-DC5>3

    authors: Alexiev BA

    更新日期:1996-11-01 00:00:00

  • Pleomorphic adenoma with bizarre myoepithelial cells: A diagnostic challenge on cytologic smear.

    abstract::The diagnosis on fine needle aspiration of salivary gland tumors with a myoepithelial component is challenging because myoepithelial cells can have a wide cytomorphologic spectrum. The authors report a case of a pleomorphic adenoma of the parotid gland that expands the spectrum of appearances that myoepithelial cells ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24301

    authors: Taweevisit M,Thorner PS

    更新日期:2019-12-01 00:00:00

  • Prognostic value of breast cancer cytologic grading: a retrospective study of 213 cases.

    abstract::The authors evaluate 213 consecutive breast cancer cases with positive fine-needle aspiration cytology. Cytologic smears were reviewed and classified according to a grading system. A correlation between cytologic grading and pathological stage (T and N category) was observed. Univariate analysis of 5-yr overall surviv...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840090210

    authors: Ciatto S,Bonardi R,Herd-Smith A,Cariaggi P,Confortini M,Bulgaresi P

    更新日期:1993-01-01 00:00:00

  • Fine needle aspiration of secondary synovial sarcoma of the thyroid gland.

    abstract::Synovial sarcomas (SS) of the head and neck region are extremely rare and arise in only 5% of cases. We present a case of secondary SS of the thyroid originally diagnosed as medullary carcinoma on fine needle aspiration (FNA). A 41-year-old man presented with several weeks of dysphonia and a left thyroid mass. FNA of ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23327

    authors: Murro D,Slade JM,Syed S,Gattuso P

    更新日期:2015-11-01 00:00:00

  • Cytological diagnosis of collagenous spherulosis of breast associated with fibroadenoma: report of a case with review of literature.

    abstract::Collagenous spherulosis of the breast is a rare incidental finding frequently associated with other benign lesions of the breast. The estimated incidence of this innocuous condition is only about 0.2% in cytological material. It is of utmost importance to differentiate collagenous spherulosis from its malignant mimics...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.20632

    authors: Gangane N,Joshi D,Anshu.,Shivkumar VB

    更新日期:2007-06-01 00:00:00

  • Fine-needle aspiration cytology of the breast in a case with abundant acellular calcific material indicative of soft-tissue calcinosis.

    abstract::Since calcific deposits in soft tissue may clinically resemble a tumor, it is feasible to investigate them by fine-needle aspiration (FNA) cytology. In this presentation we describe a case in the breast with extensive accumulation of acellular liquid calcific material which was sampled by the noninvasive technique of ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(200001)22:1<30::aid-dc9>3.

    authors: Gupta RK,Naran S,Lallu S,Fauck R,Gaskell D

    更新日期:2000-01-01 00:00:00

  • Immunocytochemical analysis of receptors for estrogen and progesterone in fine-needle aspirates from human breast carcinomas.

    abstract::Monoclonal antibodies were used for a preoperative analysis of estrogen receptors (ERs) and progesterone receptors (PgRs) in fine-needle aspirates from 44 primary human breast carcinomas. The semiquantitative receptor values obtained in cytologic specimens correlated well with those from enzyme immunoassay analysis on...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840060205

    authors: Skoog L,Humla S,Isaksson S,Tani E

    更新日期:1990-01-01 00:00:00

  • Fine-needle aspiration of breast lesions: role and accuracy in a review of 7,495 cases.

    abstract::In the past 10 years, 7,495 cytological breast fine-needle aspirations (FNAs) were performed (4,756 FNAs of solid nodes and 2,739 of cystic nodes). Of these, 2,099 cases underwent surgery; 650 (31%) had histologically proven carcinoma. Sensitivity was 83.9%, specificity was 99.5%, the predictive value for negative res...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840030206

    authors: Fessia L,Botta G,Arisio R,Verga M,Aimone V

    更新日期:1987-06-01 00:00:00

  • Chondroid syringoma diagnosed by fine needle aspiration cytology.

    abstract::Chondroid syringoma is a rare benign skin adnexal tumor of eccrine/apocrine origin affecting commonly the head and neck region. It used to be previously called as mixed tumor of skin because of the presence of both the epithelial and mesenchymal components. There are only few case reports describing the fine needle as...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21159

    authors: Kumar B

    更新日期:2010-01-01 00:00:00

  • Evaluation of flow cytometric immunophenotyping and DNA analysis for detection of malignant cells in serosal cavity fluids.

    abstract::The serosal cavities are frequent sites of tumor metastasis. The distinction between carcinoma cells, inflammatory cells, and reactive or malignant mesothelial cells can be difficult in cytology. Multicolor flow cytometry (FCM) provides the opportunity to evaluate multiple antigens simultaneously, making it possible t...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21047

    authors: Sayed DM,el-Attar MM,Hussein AA

    更新日期:2009-07-01 00:00:00

  • Cryptococcal thyroiditis: report of a case diagnosed by fine-needle aspiration cytology.

    abstract::A 54-yr-old female diabetic presented with a febrile illness and respiratory distress associated with bilateral alveolar infiltration. The left lobe of the thyroid gland was enlarged and failed to concentrate pertechnetate. The patient was euthyroid. Cryptococcus neoformans was recognized in a fine-needle aspirate and...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840070415

    authors: Vaidya KP,Lomvardias S

    更新日期:1991-01-01 00:00:00

  • Cytological features of mixed adenoneuroendocrine carcinoma of the ampulla of Vater: A case report with immunocytochemical analyses.

    abstract::Mixed adenoneuroendocrine carcinoma (MANEC) is defined as a tumor that has morphologically recognizable both adenocarcinoma and neuroendocrine carcinoma components comprising at least 30% of either components. MANEC occurring in the ampulla of Vater is extremely rare, and only 16 cases have been reported in the Englis...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23891

    authors: Yoshioka S,Ebisu Y,Ishida M,Uemura Y,Yanagimoto H,Satoi S,Tsuta K

    更新日期:2018-06-01 00:00:00

  • Cervical tuberculosis detection in Papanicolaou-stained smear: case report with review of literature.

    abstract::Genital tract tuberculosis is usually secondary to an extragenital tubercular focus. Cervical tuberculosis, however, is rare accounting for up to 3% of all genital tract diseases. The diagnosis of tuberculosis is usually established on multiple cervical biopsies followed by bacteriological confirmation. There are only...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.21082

    authors: Gupta R,Dey P,Jain V,Gupta N

    更新日期:2009-08-01 00:00:00

  • Neovaginal cytology after total pelvic exenteration for gynecological malignancies.

    abstract::Vaginal reconstruction has become an established method to restore sexual function in women who have undergone ultraradical surgery and/or irradiation for the treatment of gynecologic malignancies. Cytologic evaluation of the neovagina serves a major role in the detection of recurrent disease. The purpose of this retr...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840130106

    authors: Selvaggi SM,Haefner HK,Lelle RJ,Pearl ML,Roberts JA

    更新日期:1995-07-01 00:00:00

  • Cystic major salivary gland lesions: Utilizing fine needle aspiration to optimize the clinical management of a broad and diverse differential diagnosis.

    abstract:INTRODUCTION:The use of fine-needle aspiration (FNA) cytology for the preoperative evaluation of salivary gland lesions is an accepted but, currently, nonstandardized practice. More specifically, cystic major salivary gland lesions are relatively rare and can be very challenging to diagnose on FNA due to low cellularit...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23780

    authors: Allison DB,McCuiston AM,Kawamoto S,Eisele DW,Bishop JA,Maleki Z

    更新日期:2017-09-01 00:00:00

  • Is core needle biopsy superior to fine-needle aspiration biopsy in the diagnosis of papillary breast lesions?

    abstract::Since the 1980s core needle biopsy (CNB) has gained remarkable popularity and in many institutions it has replaced fine-needle aspiration biopsy (FNAB). However, similar to FNAB, limitation remains in the ability of this procedure to reliably diagnose a small, but prognostically significant, number of breast lesions. ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.10251

    authors: Masood S,Loya A,Khalbuss W

    更新日期:2003-06-01 00:00:00

  • Lymphoepithelial cyst (LEC) of the pancreas: cytomorphology and differential diagnosis on fine-needle aspiration (FNA).

    abstract::Lymphoepithelial cyst (LEC) of the pancreas is an extremely rare benign entity. We describe the cytopathologic findings of such a lesion in a 49-yr-old woman who was examined for epigastric pain. A trans-esophageal ultrasound-guided fine-needle aspiration of a pancreatic mass disclosed an intimate mixture of squamous ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199906)20:6<371::aid-dc8>3

    authors: Mandavilli SR,Port J,Ali SZ

    更新日期:1999-06-01 00:00:00

  • Vaginal fine-needle aspiration: A useful alternative to biopsy.

    abstract:BACKGROUND:Vaginal fine-needle aspiration (FNA) is infrequently performed to assess palpable lesions. We perform the first multi-institutional study to evaluate this procedure. METHODS:We retrospectively reviewed vaginal FNAs performed at two institutions for the past 27 years. Clinical, cytological and histological d...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23504

    authors: Lin DM,Barkan GA,Chatt G,Park JW,Gattuso P

    更新日期:2016-08-01 00:00:00

  • Fine-needle aspiration biopsy findings in sclerosing polycystic adenosis of the parotid gland.

    abstract::Sclerosing polycystic adenosis (SPA) is a recently described, rare lesion of the salivary gland analogous to fibrocystic disease of the breast. Recognition of this benign entity is important since the differential diagnosis includes other more common benign and malignant salivary gland neoplasms, particularly mucoepid...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20671

    authors: Etit D,Pilch BZ,Osgood R,Faquin WC

    更新日期:2007-07-01 00:00:00

  • Cytological features of warthin-like papillary thyroid carcinoma: A case report with review of previous cytology cases.

    abstract::Warthin-like papillary thyroid carcinoma (WLPTC) is a rare morphological variant of papillary thyroid carcinoma which mimics various benign and malignant lesions on thyroid aspiration cytology. As correct cytological diagnosis is the cornerstone for appropriate patient management, awareness of the salient cytomorpholo...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.23739

    authors: Vallonthaiel AG,Agarwal S,Jain D,Yadav R,Damle NA

    更新日期:2017-09-01 00:00:00

  • Primary hydatid cyst of the supraspinatus muscle: complete removal of the germinal layer and cytodiagnosis by fine-needle aspiration.

    abstract::Primary hydatid disease of the skeletal muscle without systemic involvement is rare. The purpose of this report is to document the novel clinical presentation and the interesting facets of fine-needle aspiration in a case of hydatid disease. It was a case of primary hydatid cyst of the left supraspinatus muscle in an ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.22925

    authors: Das DK,El-Sharawy M,Ayyash EH,Al-Enezi NA,Iqbal JR,Madda JP

    更新日期:2014-03-01 00:00:00

  • Concordance between thyroid nodule sizes measured by ultrasound and gross pathology examination: effect on patient management.

    abstract::Ultrasound examination (US) is an essential tool in the evaluation of thyroid nodules. The size determined by US is used to distinguish between clinical vs. nonclinical thyroid nodules i.e. greater than or equal to or less than 1 cm. In this study, we evaluated the concordance between the sizes of thyroid nodules meas...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20714

    authors: Deveci MS,Deveci G,LiVolsi VA,Gupta PK,Baloch ZW

    更新日期:2007-09-01 00:00:00

  • Vaginal Enterobius vermicularis diagnosed on liquid-based cytology during Papanicolaou test cervical cancer screening: A report of two cases and a review of the literature.

    abstract::Enterobiasis is one of the most common human parasitic infections. It is considered an intestinal parasite, but cases of extra-intestinal affections exist, notably infections of the female genital tract. Enterobius vermicularis (EV) eggs (or ova) have been found in the cervical smears of two patients in our institute ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.23812

    authors: Tsai CY,Junod R,Jacot-Guillarmod M,Beniere C,Ziadi S,Bongiovanni M

    更新日期:2018-02-01 00:00:00

  • Intraductal carcinoma in a male breast: diagnosis by nipple discharge cytology.

    abstract::We describe a case of intraductal carcinoma (comedo type) in a 50-yr-old male. The patient had presented with a bloody nipple discharge, which had shown malignant cells on cytologic examination. In current clinical practice, the use of nipple discharge cytology as a screening and diagnostic tool for breast carcinoma i...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840120414

    authors: Hirschman SA,Hoda SA,May M,Cody HS 3rd

    更新日期:1995-06-01 00:00:00

  • Intranuclear peudoinclusions: morphology, pathogenesis, and significance.

    abstract::Intranuclear pseudoinclusions represent invaginations of the cytoplasm into the nucleus. The nuclear pseudoinclusions are generally easier to appreciate in cytological preparations than frozen sections or histopathology thus forming an important cytological feature. In the present article we have reviewed the morpholo...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.21714

    authors: Arora SK,Dey P

    更新日期:2012-08-01 00:00:00

  • Diagnostic role of fine-needle aspiration of pancreatic allograft to detect rejection.

    abstract::The purpose of this study was to assess whether the same principles to evaluate renal transplant by fine-needle aspiration (FNA) for rejection could be applied to pancreatic allograft. Between 1996-1998, 25 ultrasound-guided FNAs on 13 patients with pancreatic allograft were performed and ThinPrep made. The percentage...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.10179

    authors: Sariya D,Kluskens L,Assad L,Treaba D,Reddy V,Gattuso P

    更新日期:2002-11-01 00:00:00

  • Mesothelioma: you do not have to work for it.

    abstract::Asbestos pollution is a global problem. Asbestos exposure induced mesothelioma does not require an 'occupational' type of exposure. Bystander exposures may result in earlier age of disease onset and more aggressive disease progression as described in the following 3 case reports. ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20766

    authors: Ampleford EJ,Ohar J

    更新日期:2007-12-01 00:00:00

  • Liesegang rings in renal cyst fluid.

    abstract::Peculiar ring-like structures identified as Liesegang rings (LRs) were found in renal cyst fluid from three patients with benign renal cysts. They ranged in size from 5 to 820 mu. Most had a double-layer outer wall with equally spaced radial cross-striations and an amorphous central nidus. Special stains were performe...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840060310

    authors: Katz LB,Ehya H

    更新日期:1990-01-01 00:00:00