Abstract:
:The whole cell patch-clamp technique in combination with the slice preparation was used to investigate the electrophysiological properties of pigeon semicircular canal sensory and supporting cells. These properties were also characterized in regenerating neuroepithelia of pigeons preinjected with streptomycin to kill the hair cells. Type II hair cells from each of the three semicircular canals showed similar, topographically related patterns of passive and active membrane properties. Hair cells located in the peripheral regions (zone I, near the planum semilunatum) had less negative resting potentials [0-current voltage in current-clamp mode (Vz) = -62.8 +/- 8.7 mV, mean +/- SD; n = 13] and smaller membrane capacitances (Cm = 5.0 +/- 0.9 pF, n = 14) than cells of the intermediate (zone II; Vz = -79.3 +/- 7.5 mV, n = 3; Cm = 5.9 +/- 1.2 pF, n = 4) and central (zone III; Vz = -68.0 +/- 9.6 mV, n = 17; Cm = 7.1 +/- 1.5 pF, n = 18) regions. In peripheral hair cells, ionic currents were dominated by a rapidly activating/inactivating outward K+ current, presumably an A-type K+ current (IKA). Little or no inwardly rectifying current was present in these cells. Conversely, ionic currents of central hair cells were dominated by a slowly activating/inactivating outward K+ current resembling a delayed rectifier K+ current (IKD). Moreover, an inward rectifying current at voltages negative to -80 mV was present in all central cells. This current was composed of two components: a slowly activating, noninactivating component (Ih), described in photoreceptors and saccular hair cells, and a faster-activating, partially inactivating component (IK1) also described in saccular hair cells in some species. Ih and IK1 were sometimes independently expressed by hair cells. Hair cells located in the intermediate region (zone II) had ionic currents more similar to those of central hair cells than peripheral hair cells. Outward currents in intermediate hair cells activated only slightly more quickly than those of the cells of the central region, but much more slowly than those of the peripheral cells. Additionally, intermediate hair cells, like central hair cells, always expressed an inward rectifying current. The regional distribution of outward rectifying potassium conductances resulted in macroscopic currents differing in peak-to-steady state ratio. We quantified this by measuring the peak (Gp) and steady-state (Gs) slope conductance in the linear region of the current-voltage relationship (-40 to 0 mV) for the hair cells located in the different zones. Gp/Gs average values (4.1 +/- 2.1, n = 15) from currents in peripheral hair cells were higher than those from intermediate hair cells (2.3 +/- 0.8, n = 4) and central hair cells(1.9 +/- 0.8, n = 21). The statistically significant differences (P < 0.001) in Gp/Gs ratios could be accounted for by KA channels being preferentially expressed in peripheral hair cells. Hair cell electrophysiological properties in animals pretreated with streptomycin were investigated at approximately 3 wk and approximately 9-10 wk post injection sequence (PIS). At 3 wk PIS, hair cells (all zones combined) had a statistically significantly (P < 0.001) lower Cm (4.6 +/- 1.1 pF, n = 24) and a statistically significantly (P < 0.01) lower Gp(48.4 +/- 20.8 nS, n = 26) than control animals (Cm = 6.2 +/- 1.6 pF, n = 36; Gp = 66 +/- 38.9 nS, n = 40). Regional differences in values of Vz, as well as the distribution of outward and inward rectifying currents, seen in control animals, were still obvious. But, differences in the relative contribution of the expression of the different ionic current components changed. This result could be explained by a relative decrease in IKA compared with IKD during that interval of regeneration, which was particularly evident in peripheral hair cells. (ABSTRACT TRUNCATED)
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Masetto S,Correia MJdoi
10.1152/jn.1997.78.4.1913subject
Has Abstractpub_date
1997-10-01 00:00:00pages
1913-27issue
4eissn
0022-3077issn
1522-1598journal_volume
78pub_type
杂志文章abstract::The aim of this study was to examine how cat spinal motoneurons integrate the synaptic currents generated by the concurrent activation of large groups of presynaptic neurons. We obtained intracellular recordings from cat triceps surae motoneurons and measured the effects of repetitive activity in different sets of pre...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2000.83.1.483
更新日期:2000-01-01 00:00:00
abstract::Abducens neurons undergo a dose-dependent synaptic blockade (either disinhibition or complete blockade) when tetanus neurotoxin (TeNT) is injected into the lateral rectus muscle at either a low (0.5) or a high dose (5 ng/kg). We studied the firing pattern and recruitment order in abducens neurons both in control and a...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00402.2003
更新日期:2003-10-01 00:00:00
abstract::Understanding how the brain processes sensory input to generate behavior remains an important problem in neuroscience. Towards this end, it is useful to compare results obtained across multiple species to gain understanding as to the general principles of neural coding. Here we investigated hindbrain pyramidal cell ac...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00594.2016
更新日期:2016-12-01 00:00:00
abstract::Evidence first described in reduced animal models over 100 years ago led to deductions about the control of locomotion through spinal locomotor central pattern-generating (CPG) networks. These discoveries in nature were contemporaneous with another form of deductive reasoning found in popular culture, that of Arthur C...
journal_title:Journal of neurophysiology
pub_type: 杂志文章,评审
doi:10.1152/jn.00554.2017
更新日期:2018-07-01 00:00:00
abstract::The basal forebrain plays important roles in arousal, learning, and memory by stimulating cortical activation characterized by rhythmic slow theta and high-frequency beta-gamma activities. Although cholinergic neurons play a significant part in these roles, other, including GABAergic, neurons appear to contribute. Usi...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00938.2002
更新日期:2003-02-01 00:00:00
abstract::Responses have been recorded from primary endings of muscle spindles in the cat soleus muscle. Changes in spindle responsiveness were measured following a period of conditioning that consisted of a series of rapid stretches or of tetanic ventral root stimulation. In the testing procedure the response of a single spind...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1986.56.2.451
更新日期:1986-08-01 00:00:00
abstract::Numerous models of the oculomotor neuronal integrator located in the prepositus hypoglossi nucleus (PHN) involve both highly tuned recurrent networks and intrinsic neuronal properties; however, there is little experimental evidence for the relative role of these two mechanisms. The experiments reported here show that ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01355.2005
更新日期:2006-07-01 00:00:00
abstract::1. Synaptic interactions between identified neurons in the flight system of the locust were investigated by the use of standard intracellular recording and staining techniques. The intent was to determine the distribution and functional significance of delayed excitatory connections, which have been previously describ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1991.65.5.1150
更新日期:1991-05-01 00:00:00
abstract::During auditory stimulation with several frequency components, distortion products (DPs) are generated as byproduct of nonlinear cochlear amplification. After generated, DP energy is reemitted into the ear channel where it can be measured as DP otoacoustic emission (DPOAE), and it also induces an excitatory response a...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.90805.2008
更新日期:2009-03-01 00:00:00
abstract::1. We recorded single cell activities in the caudate nucleus of the monkeys trained to perform a series of visuomotor tasks. In the first part of this paper, we summarize the types and locations of neurons in the monkey caudate nucleus. In the second part, we report the characteristics of neurons related to saccadic e...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1989.61.4.780
更新日期:1989-04-01 00:00:00
abstract::1. We examined the role of gamma-aminobutyric acid (GABA)-mediated inhibition in shaping excitatory tuning curves and creating selectivity for frequency-modulated (FM) sweeps in 29 neurons in the central nucleus of the inferior colliculus (ICC) of the pallid bat, with the use of single-unit recording coupled with the ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1996.76.2.1059
更新日期:1996-08-01 00:00:00
abstract::Intracellular recordings were performed in Cs-loaded sympathetic preganglionic neurons (SPNs) of the intermediolateral nucleus, identified by antidromic stimulation, in the slice of the T2 or T3 segment of the cat spinal cord. Loading the neurons with Cs resulted in broadening of the action potential, depression of th...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1987.57.5.1325
更新日期:1987-05-01 00:00:00
abstract::We have examined the role of dorsal horn cells that respond to Lissauer tract stimulation in regulating primary afferent depolarization (PAD). PAD was monitored by recording the dorsal root potential (DRP) in the roots of the lumbar cord. Recordings were made of the discharges of Lissauer tract-responsive cells, and t...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1998.80.2.667
更新日期:1998-08-01 00:00:00
abstract::Two forms of undulatory locomotion in the lamprey (a lower vertebrate) have been described earlier: fast forward swimming (FFS) used for long distance migrations and slow backward swimming (SBS) used for escape from adverse tactile stimuli. In the present study, we describe another form of escape behavior: slow forwar...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00903.2007
更新日期:2008-01-01 00:00:00
abstract::Single-unit recordings were made from layer II/III anterior piriform cortex (aPCX) neurons in adult Wistar rats to examine odor response patterns to unilaterally and bilaterally delivered stimuli. Isoamyl acetate odor stimulation was presented either unilaterally through tubes inserted into the external nares, or bila...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1997.78.1.160
更新日期:1997-07-01 00:00:00
abstract::1. Fluorescence changes in the dye di-4-ANEPPS were monitored on the rat's nasal septum and medial surface of the turbinates in response to odorant stimuli. For each mucosal surface a 6.0 x 6.0-mm area was sampled at 100 contiguous sites with a 10 x 10 photodiode array. The odorants were propyl acetate, 2-propanol, ci...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1995.73.1.387
更新日期:1995-01-01 00:00:00
abstract::Spatial and temporal properties of LGN receptive fields were studied by flashing a small bar of light across the field in 28 discrete steps. The flashes at each of the spatial positions were used to produce 28 PST histograms. These histograms were in turn displayed as a plane, with space on the chi axis, time on the p...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1976.39.2.213
更新日期:1976-03-01 00:00:00
abstract::Although metabotropic glutamate receptors (mGluRs) mGluR1 and mGluR5 are often found to have similar functions, there is considerable evidence that the two receptors also serve distinct functions in neurons. In hippocampal area CA1, mGluR5 has been most strongly implicated in long-term synaptic depression (LTD), where...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00383.2005
更新日期:2006-04-01 00:00:00
abstract::The suprachiasmatic nucleus (SCN) receives a dense serotonergic innervation that modulates photic input to the SCN via serotonin 1B (5-HT1B) presynaptic receptors on retinal glutamatergic terminals. However, the majority of 5-HT1B binding sites in the SCN are located on nonretinal terminals and most axonal terminals i...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00770.2004
更新日期:2005-06-01 00:00:00
abstract::The tail and paws in rodents are heat exchangers involved in the maintenance of core body temperature (T(core)). They are also the most widely used target organs to study acute or chronic "models" of pain. We describe the fluctuations of vasomotor tone in the tail and paws in conditions of thermal neutrality and the c...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00721.2013
更新日期:2014-11-01 00:00:00
abstract::1. The discharge patterns and axonal projections of saccadic long-lead burst neurons (LLBNs) with somata in the pontine reticular formation were studied in alert squirrel monkeys with the use of the method of intraaxonal recording and horseradish peroxidase injection. 2. The largest population of stained neurons were ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1996.76.1.353
更新日期:1996-07-01 00:00:00
abstract::We have used a computer-based mathematical model of alpha-motoneurons and of group Ia synaptic input to them, based on anatomical and electrophysiological data from the cat spinal cord, in order to examine the effects of variations in neuron size and input resistance and of conductance magnitude and duration on the ge...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1983.50.2.399
更新日期:1983-08-01 00:00:00
abstract::N-arachidonoyldopamine (NADA) is an endogenous molecule found in the nervous system that is capable of acting as a vanilloid agonist via the TRPV1 receptor and as a cannabinoid agonist via the CB1 receptor. Using anesthetized rats, we investigated the neural correlates of behavioral thermal hyperalgesia produced by NA...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00395.2005
更新日期:2006-02-01 00:00:00
abstract::Responses of low characteristic-frequency (CF) neurons in the inferior colliculus were obtained to amplitude-modulated (AM) high-frequency tones in which the modulation rate was equal to the neuron's CF. Despite all spectral components lying outside the pure tone-evoked response areas, discharge rates were modulated b...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00034.2004
更新日期:2004-09-01 00:00:00
abstract::1. Depolarization-induced elevations of intracellular calcium concentration ([Ca2+]i) were examined in slice-cultured hippocampal pyramidal and nonpyramidal cells of the CA3 region by combined intracellular and multisite fura-2 recording techniques. 2. In pyramidal cells, spiking activity induced by depolarizing curre...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1992.68.3.961
更新日期:1992-09-01 00:00:00
abstract::Recent studies have pointed to olfactory system beta oscillations of the local field potential (15-30 Hz) and their roles both in learning and as specific responses to predator odors. To describe odorant physical properties, resultant behavioral responses and changes in the central olfactory system that may induce the...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00124.2007
更新日期:2007-07-01 00:00:00
abstract::Weight predictions used to scale lifting forces adapt quickly when repeatedly lifting unusually weighted objects and are readily updated by explicit information provided about weight. In contrast, weight predictions used when making perceptual judgments about weight are more resistant to change and are largely unaffec...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01051.2015
更新日期:2016-09-01 00:00:00
abstract::We have addressed the source and nature of the persistent neural activity that bridges the stimulus-free gap between the conditioned stimulus (CS) and unconditioned stimulus (US) during trace eyelid conditioning. Previous work has demonstrated that this persistent activity is necessary for trace eyelid conditioning: C...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00689.2011
更新日期:2012-01-01 00:00:00
abstract::1. Microelectrode recordings from 15 slowly adapting (SA) cutaneous mechanoreceptor afferents originating in hairy skin were obtained from the radial nerve in humans. 2. Controlled skin stretch was applied to the back of the hand that encompassed the physiological range of skin stretch during movements at the metacarp...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1992.67.5.1105
更新日期:1992-05-01 00:00:00
abstract::The temporal lobe is well known for its oscillatory activity associated with exploration, navigation, and learning. Intrinsic membrane potential oscillations (MPOs) and resonance of stellate cells (SCs) in layer II of the entorhinal cortex are thought to contribute to network oscillations and thereby to the encoding o...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00743.2011
更新日期:2013-01-01 00:00:00