Abstract:
:To examine mechanisms responsible for the long-term regulation of Ca2+-channels in an adult neuron, changes in whole cell Ba2+ current (IBa) were examined in adult bullfrog sympathetic ganglion B cells in vitro. Cells were cultured at low density in defined, serum free medium. After 15 days, total IBa was similar to the initial value, whereas IBa density was reduced by approximately 36%, presumably due to an increase in neuronal surface area. By contrast, IBa density remained constant after 6-15 days in the presence of murine beta-NGF (200 ng/ml), and total IBa was almost doubled. Inclusion of cytosine arabinoside (Ara-C; 10 microM) to inhibit proliferation of nonneuronal cells, did not affect the survival of neurons in the absence of nerve growth factor (NGF) nor did it attenuate IBa. Ara-C did not prevent the effect of NGF on IBa. There were three independent components to the action of NGF; during 6-9 days, it increased omega-conotoxin-GVIA-sensitive N-type IBa (IBa,N); increased nifedipine-sensitive L-type IBa (IBa,L) and decreased inactivation of the total Ba2+ conductance (gBa). The latter effect involved a selective decrease in the amplitude of one of the four kinetic components that describe the inactivation process. Total IBa was also 55.8% larger than control in the somata of B cells acutely dissociated from leopard frogs that had received prior subcutaneous injections of NGF. By contrast, injection of NGF antiserum decreased total IBa by 29.4%. There was less inactivation of gBa in B cells from NGF-injected animals than in cells from animals injected with NGF antiserum (P < 0.001). These data suggest that NGF-like molecule(s) play(s) a role in the maintenance of IBa in an adult amphibian sympathetic neuron; the presence of NGF may allow the neuron to maintain a constant relationship between cell size and current density. They also show that IBa inactivation in an adult neuron can be modulated in a physiologically relevant way by an extracellular ligand.
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Lei S,Dryden WF,Smith PAdoi
10.1152/jn.1997.78.6.3359subject
Has Abstractpub_date
1997-12-01 00:00:00pages
3359-70issue
6eissn
0022-3077issn
1522-1598journal_volume
78pub_type
杂志文章abstract::1. Effects of substance P (SP) and other tachykinins on membrane currents were investigated using whole cell voltage clamp in cultured sensory neurons isolated from rat dorsal root ganglia. 2. SP (100 nM) evoked an inward current in two-thirds of the cells at negative potentials. In most of the cells that generated th...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
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abstract::1. Our previous study showed that two distinct postural modifications occurred when subjects were instructed to step, rather than maintain stance, in response to a backward surface translation: 1) the automatic postural responses to the surfaces perturbation were reduced in magnitude and 2) the anticipatory postural a...
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
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更新日期:1983-03-01 00:00:00
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更新日期:1998-03-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1984-09-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1152/jn.1996.75.1.38
更新日期:1996-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-09-15 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
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更新日期:1991-03-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2011-03-01 00:00:00
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pub_type: 杂志文章
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