当前位置： SCI文献检索 > ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA期刊下所有文献 > The use of MALDI-TOF ICMS as an alternative tool for Trichophyton rubrum identification and typing.

The use of MALDI-TOF ICMS as an alternative tool for Trichophyton rubrum identification and typing.

Abstract：

AIMS:In this study, the potential of matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry (MALDI-TOF ICMS) was investigated for the identification of clinical isolates. The isolates were analyzed at the species and strain level. METHODS:Spectral identification by MALDI-TOF ICMS was performed for all strains, and compared with the results of sequencing of the internal transcribed spacers (ITS1 and ITS2), and the 5.8S rDNA region. PCR fingerprinting analysis using primers M13, (GACA)4, and (AC)10 was performed in order to assess the intra-specific variability of Trichophyton rubrum strains. RESULTS:The identification of strains at species level by MALDI-TOF ICMS was in agreement with the previously performed morphological and biochemical analysis. Sequence data confirmed spectral mass identification at species level. Intra-specific variability was assessed. Within the T. rubrum cluster, strains were distributed into smaller highly related sub-groups with a similarity values above 85%. CONCLUSIONS:MALDI-TOF ICMS was shown to be a rapid, low-cost and accurate alternative tool for the identification and strain typing of T. rubrum.

journal_name

Enferm Infecc Microbiol Clin

journal_title

Enfermedades infecciosas y microbiologia clinica

authors

Pereira L,Dias N,Santos C,Lima N

doi

10.1016/j.eimc.2013.01.009

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

11-7

issue

1

eissn

0213-005X

issn

1578-1852

pii

S0213-005X(13)00014-1

journal_volume

32

pub_type

杂志文章

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