Abstract:
BACKGROUND:It has been observed an spectacular increasing of opportunistic Candida yeast infections. Many of them are fatal, and rapid and effective identification of the infecting species contributes to start the correct treatment. Several new methods for yeast identification have become available; Microring YT is one of these methods based on the growth inhibition by 6 different chemical products. The aim of this work is to study the performance of the test using representative clinical yeast isolates. METHODS:A total of 146 strains belonging to the 5 most common Candida species isolated in the clinical laboratory were identified using conventional methods (germ tube and chlamydospores production, and the standard API 20C AUX and 16 sugars auxonography; Institute Pasteur) and the Microring YT System. This test uses the differing susceptibilities of yeast to 6 discs mounted on a filter paper ring. The chemical products and dyes are: janus green, ethidium bromide, triphenyl tetrazolium chloride, brilliant green, cycloheximide and rhodamine 6G. The inhibition pattern of a 6 digit code is compared with a list of profiles. RESULTS:Using the Microring YT system 112 of the 146 studied strains were correctly identified with an overall concordance of 77% between this method and the standard one. The morphological study (germ tube production) increased 6% the identification of Candida albicans. Better results were obtained with C. krusei and C. parapsilosis (85% of concordance). With C. glabrata only 59% of concordance was found. CONCLUSIONS:In spite Microring YT is a simple method, easy to perform and read, it was considered inadequate for the identification of Candida species as a routine microbiological procedure.
journal_name
Enferm Infecc Microbiol Clinjournal_title
Enfermedades infecciosas y microbiologia clinicaauthors
Torres-Rodríguez JM,Montsant-Montané L,Madrenys-Brunet Nsubject
Has Abstractpub_date
1994-11-01 00:00:00pages
439-42issue
9eissn
0213-005Xissn
1578-1852journal_volume
12pub_type
杂志文章abstract:BACKGROUND:To analyze the production and repercussions of the scientific activity of Spanish authors in the fields of microbiology, mycology, parasitology and virology. METHODS:Documents published during the period of 1990 to 2000 compiled on the MEDLINE database >(WEBSPIRS 4.2) were retrieved using the following sear...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1157/13078799
更新日期:2005-08-01 00:00:00
abstract:BACKGROUND:Diarrhea associated with Clostridium difficile is a health care problem of growing importance in the last few years specially in the hospital environment. The epidemiologic data and factors associated with this disease have not, to date, been sufficiently studied in Spain. METHODS:The cases of diarrhea asso...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
abstract:BACKGROUND:Spain, together with the other southern European countries, was considered to be an area with a moderate degree of endemia. This fact has consequences for tourists that visit these areas and for vaccination strategies. A prevalence study was proposed in order to get to know the situation of this infection in...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/s0213-005x(01)72686-9
更新日期:2001-11-01 00:00:00
abstract::Because lifelong therapy is needed in HIV infection, high long-term adherence is necessary. Darunavir/co-bicistat/emtricitabina/tenofovir alafenamide (Symtuza®) is the first triple therapy combining a protease inhibitor (PI) in a single tablet regimen. This drug combines the potency and high genetic barrier of the mos...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/S0213-005X(18)30395-1
更新日期:2018-12-01 00:00:00
abstract:INTRODUCTION:The increase in infections due to dermatophytes in recent years led us to study the effectiveness of new antifungal formulations against these microorganisms. METHODS:The in vitro activity of a new antifungal agent, voriconazole, was compared with three other antifungal agents, itraconazole, fluconazole a...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/s0213-005x(03)72992-9
更新日期:2003-11-01 00:00:00
abstract:INTRODUCTION:The use of the matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry has shown to be effective and fast in some clinical specimens for the identification of colonizing microorganisms. The objective of the study was to analyze the validity values for the prediction of colo...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
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更新日期:2014-06-01 00:00:00
abstract:INTRODUCTION:To characterize OXA-48 carbapenemase-producing Klebsiella pneumoniae strains isolated after an increase in carbapenem resistance in Catalonia. METHODOLOGY:K. pneumoniae identification, antimicrobial susceptibility studies, the Modified Hodge Test method, amplification of antimicrobial resistance genes (ag...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2018.02.003
更新日期:2019-02-01 00:00:00
abstract::The alarms that have sounded in the last two decades of the 20(th) century in the feld of antimicrobial resistance in livestock farming have led to the development of various measures that have substantially improved the use of antimicrobial agents in veterinary medicine. These measures mainly involve the implementati...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/S0213-005X(10)70042-2
更新日期:2010-11-01 00:00:00
abstract::In the present study the 60 patients with brucellosis and evaluation of bone marrow aspirate seen at the Hospital Base Cayetano Heredia from 1980 to 1986 were included. Iron deficiency was found in the bone marrow in 34.5% of patients, 31% in males and 36% in females. No correlation was found between iron deficiency a...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::The relevance of mixed genital infections in our area was evaluated, and we report their clinical and epidemiological features for both viral types. We evaluated overall 54 Herpes simplex virus (HSV) isolates from 44 patients seen at the clinic of sexually transmitted diseases (STD) of the Sevilla School of Medicine d...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
abstract:OBJECTIVE:To describe the changes in causes of death among persons with HIV infection. METHODS:An analysis of mortality according to cause was performed in persons diagnosed with HIV infection and residing in the province of Navarre (Spain) from 1985 to 2004. RESULTS:Among 1,649 persons diagnosed with HIV infection u...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1157/13096746
更新日期:2007-01-01 00:00:00
abstract::The detection of group B streptococci by the ICON Strep-B test an enzyme immunoassay, and culture in new Granada Medium was compared in tubes and plates in a total of 200 vaginal specimens from pregnant women. The group B streptococci were cultured in new Granada medium from 33 of these specimens (incidence of 16.5%)....
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
abstract:BACKGROUND:The evaluation of health care workers role in methicillin-resistant Staphylococcus aureus outbreaks and the efficacy of mupirocin as a topical agent for nasal carriers. METHOD:Microbiologic study of nasal microflora of 1547 health care workers from the San Carlos University Hospital and 108 health care work...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1992-02-01 00:00:00
abstract::The fimbriated adhesines constitute a factor of virulence of E. coli in the urinary tract infections. The presence of type 1 fimbriae and fimbriae P was evaluated in 23 E. coli strains isolated with patients with chronic bacterial prostatitis. For the diagnosis of prostatitis the Stamey test was used. The detection of...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1989-03-01 00:00:00
abstract:INTRODUCTION:To investigate the susceptibility of group B streptococci (GBS) to macrolides and lincosamides and assess alternatives for intrapartum chemoprophylaxis in women allergic to penicillin and colonized by a GBS strain resistant to these antibiotics. METHODS:Multicenter study performed with 131 strains isolate...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,多中心研究
doi:10.1157/13059822
更新日期:2004-05-01 00:00:00
abstract::Antimicrobial prophylaxis in surgery refers to a very brief course of an antimicrobial agent initiated just before the start of the procedure. The efficacy of antimicrobials to prevent postoperative infection at the site of surgery (incisional superficial, incisional deep, or organ/space infection) has been demonstrat...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:2002-08-01 00:00:00
abstract::The use of intravascular devices in the usual and daily clinical practice, used as diagnostic or as therapeutic means, is a reality nowadays, present in all hospitals of our medium, and it is increasing. In the area of intensive care, it constitutes a routine and prolonged procedure, and its contribution within the ho...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1997-10-01 00:00:00
abstract::Quinolone resistance is caused mainly by chromosomal mutations in gram negative bacteria. In 1998, plasmid-mediated resistance to quinolones in clinical isolates was first reported in a Klebsiella pneumoniae strain. Locus qnr (quinolone resistance) was responsible of the quinolone resistance in this plasmid. qnr codes...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:10.1157/13070406
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND:HIV infection and parenteral drug abuse (PDA) are frequently associated conditions. Both are at increased risk of developing specific types of Candida infections. Localized CNS infection is an exceptional finding. METHODS:We report two cases of meningitis in PDA due to Candida albicans--one of them HIV posi...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
doi:
更新日期:1993-05-01 00:00:00
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journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2015.07.006
更新日期:2016-05-01 00:00:00
abstract:BACKGROUND:Several recent studies have shown that the combination of ddI plus TDF can produce an unexpected drop in CD4 cell counts, even after correcting the ddI dose. OBJECTIVE:Comparative study of the immunological effectiveness of various once-daily NRTI backbones (ddI plus TDF, ddI plus 3TC or TDF plus 3TC) in an...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1157/13091779
更新日期:2006-08-01 00:00:00
abstract::The development of novel direct antiviral agents (DAAs) against hepatitis C virus (HCV) has represented a breakthrough in the treatment of chronic hepatitis C. Telaprevir and boceprevir are the first two protease inhibitor (PI) DAAs to be approved for combination therapy with pegylated interferon (PEG-IFN) and ribavir...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/S0213-005X(13)70113-7
更新日期:2013-02-01 00:00:00
abstract:BACKGROUND:Extrapulmonary tuberculosis is a relatively frequent disease in patients with HIV infection. This may be observed coinciding with pulmonary involvement or without diagnostic data of the same. Within extrapulmonary involvement, gastrointestinal involvement is one of the least frequently observed and its diagn...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract:INTRODUCTION:Primary lymphoma is the most common neoplasm of the central nervous system (CNS) in AIDS patients. METHODS:We retrospectively reviewed the clinical manifestations, neuroimaging findings, diagnostic methods used, histological characteristics, detection of Epstein-Barr virus (EBV) DNA by PCR in cerebrospina...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章,评审
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更新日期:2004-06-01 00:00:00
abstract:OBJECTIVE:To determine the prevalence of chronic colonization with methicillin-resistant Staphylococcus aureus (MRSA) in patients with cystic fibrosis, describe antibiotic sensitivity of the strains, and compare the patients' clinical characteristics with those of patients infected with methicillin-sensitive S. aureus ...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2008.02.008
更新日期:2009-02-01 00:00:00
abstract::The effectiveness of different methodologies to identify antigenemia and antibodies against HIV at the time of viral primary infection was evaluated. To this end, 6 consecutive serum samples of a patient who had had symptomatic seroconversion were assessed. The detection of antigenemia was earlier than that of antibod...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract:BACKGROUND:It has been established that the genomic background of Mycobacterium tuberculosis may influence disease progression, in particular for the Beijing family and the Latin American and Mediterranean (LAM)/RDRio strains. The purpose of this study was to evaluate the prevalence of the LAM/RDRio genotype in cases o...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2020.07.003
更新日期:2020-08-24 00:00:00
abstract:BACKGROUND:Staphylococcus aureus is the main pathogen causing nosocomial infections. Health professionals, including medical students, could be a source of transmission. The aims of the study were to determine the rate of nasal carriage of S.aureus susceptible and resistant to methicillin (MRSA) and evaluate the knowle...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2012.12.005
更新日期:2013-10-01 00:00:00
abstract:INTRODUCTION:Streptococcus suis (S. suis) infection is poorly described zoonosis in our country, which is related with exposure to pigs or their meat. The most common clinical presentation is meningitis, while spinés involvement is rare. METHODS:We report 2 cases of S. suis infection and perform a systematic review of...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2020.08.005
更新日期:2020-10-14 00:00:00
abstract:PURPOSE:Survival in people living with HIV (PLWH) has increased and thus people are aging with HIV, increasing the frequency of multimorbidity and polypharmacy. This cross-sectional study was conducted to evaluate the prevalence of polypharmacy among PLWH who were on antiretroviral treatment and were followed in an out...
journal_title:Enfermedades infecciosas y microbiologia clinica
pub_type: 杂志文章
doi:10.1016/j.eimc.2020.09.020
更新日期:2020-11-20 00:00:00