Abstract:
BACKGROUND:Cardiotoxicity is a leading cause for drug attrition during pharmaceutical development and has resulted in numerous preventable patient deaths. Incidents of adverse cardiac drug reactions are more common in patients with preexisting heart disease than the general population. Here we generated a library of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) from patients with various hereditary cardiac disorders to model differences in cardiac drug toxicity susceptibility for patients of different genetic backgrounds. METHODS AND RESULTS:Action potential duration and drug-induced arrhythmia were measured at the single cell level in hiPSC-CMs derived from healthy subjects and patients with hereditary long QT syndrome, familial hypertrophic cardiomyopathy, and familial dilated cardiomyopathy. Disease phenotypes were verified in long QT syndrome, hypertrophic cardiomyopathy, and dilated cardiomyopathy hiPSC-CMs by immunostaining and single cell patch clamp. Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) and the human ether-a-go-go-related gene expressing human embryonic kidney cells were used as controls. Single cell PCR confirmed expression of all cardiac ion channels in patient-specific hiPSC-CMs as well as hESC-CMs, but not in human embryonic kidney cells. Disease-specific hiPSC-CMs demonstrated increased susceptibility to known cardiotoxic drugs as measured by action potential duration and quantification of drug-induced arrhythmias such as early afterdepolarizations and delayed afterdepolarizations. CONCLUSIONS:We have recapitulated drug-induced cardiotoxicity profiles for healthy subjects, long QT syndrome, hypertrophic cardiomyopathy, and dilated cardiomyopathy patients at the single cell level for the first time. Our data indicate that healthy and diseased individuals exhibit different susceptibilities to cardiotoxic drugs and that use of disease-specific hiPSC-CMs may predict adverse drug responses more accurately than the standard human ether-a-go-go-related gene test or healthy control hiPSC-CM/hESC-CM screening assays.
journal_name
Circulationjournal_title
Circulationauthors
Liang P,Lan F,Lee AS,Gong T,Sanchez-Freire V,Wang Y,Diecke S,Sallam K,Knowles JW,Wang PJ,Nguyen PK,Bers DM,Robbins RC,Wu JCdoi
10.1161/CIRCULATIONAHA.113.001883subject
Has Abstractpub_date
2013-04-23 00:00:00pages
1677-91issue
16eissn
0009-7322issn
1524-4539pii
CIRCULATIONAHA.113.001883journal_volume
127pub_type
杂志文章相关文献
CIRCULATION文献大全abstract:BACKGROUND:Endogenous nitric oxide (NO) reduces sympathetic vasoconstriction by attenuating neuronal excitability in the brain stem and inhibition of postganglionic neurotransmission. We studied whether this modulation of sympathetic circulatory control by NO may be altered during chronic administration of NO donor dru...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.97.23.2352
更新日期:1998-06-16 00:00:00
abstract::Values for several coronary risk factors, including systolic and diastolic blood pressure, serum cholesterol, triglycerides, blood glucose, uric acid, hemoglobin, weight, vitral capacity and cigarette smoking have been found to be similar among spouses in the Framingham Study. However, longitudinal analyses show that...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.52.4.589
更新日期:1975-10-01 00:00:00
abstract::Two guidelines from the American College of Cardiology (ACC), the American Heart Association (AHA), and collaborating societies address the risk of aortic dissection in patients with bicuspid aortic valves and severe aortic enlargement: the "2010 ACCF/AHA/AATS/ACR/ASA/SCA/SCAI/SIR/STS/SVM Guidelines for the Diagnosis ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIR.0000000000000331
更新日期:2016-02-16 00:00:00
abstract::We assessed the effects of ethanol and autonomic blockade on left ventricular function in nine normal subjects, age 20--35 years, using M-mode echocardiography and systolic time intervals. On day 1, measurements were made of heart rate, mean velocity of circumferential fiber shortening, and left ventricular pre-ejecti...
journal_title:Circulation
pub_type: 临床试验,杂志文章
doi:10.1161/01.cir.59.1.120
更新日期:1979-01-01 00:00:00
abstract:BACKGROUND:It has been suggested that intracellular Ca2+ overload in cardiac myocytes leads to the development of diabetic cardiomyopathy. Troglitazone, an insulin-sensitizing agent, is a promising therapeutic agent for diabetes and has been shown to prevent diabetes-induced myocardial changes. To elucidate the underly...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.99.22.2942
更新日期:1999-06-08 00:00:00
abstract:BACKGROUND:Creation of a complete bidirectional inferior vena cava-tricuspid annulus isthmus block (CBIB) by radiofrequency catheter ablation is now a well-accepted criterion for prevention of common atrial flutter (AFl) recurrences. However, some patients still complain of palpitations after ablation, and it is not kn...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.99.4.534
更新日期:1999-02-02 00:00:00
abstract:BACKGROUND:A low level of HDL cholesterol has been identified as a risk factor for stroke in observational studies. METHODS AND RESULTS:Our objective was to determine whether treatment aimed at raising HDL cholesterol and lowering triglycerides reduces stroke in men with coronary heart disease and low levels of both H...
journal_title:Circulation
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1161/01.cir.103.23.2828
更新日期:2001-06-12 00:00:00
abstract::We determined the pharmacokinetics, efficacy and therapeutic plasma concentration of encainide, a new antiarrhythmic drug that affects His-Purkinje conduction but not ventricular refractoriness. Nine patients with frequent and complex premature ventricular complexes were studied in a 3-day double-blind protocol. Each ...
journal_title:Circulation
pub_type: 临床试验,杂志文章
doi:10.1161/01.cir.64.2.290
更新日期:1981-08-01 00:00:00
abstract:BACKGROUND:Apoptosis contributes to cell loss after ischemia/reperfusion injury in the heart. This study describes the time course and level of apoptosis in different cell types in the intact heart during ischemia/reperfusion injury. METHODS AND RESULTS:Isolated Langendorff-perfused rat hearts were subjected to perfus...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.104.3.253
更新日期:2001-07-17 00:00:00
abstract:BACKGROUND:Titin is a giant elastic protein that spans the half-sarcomere from Z-disk to M-band. It acts as a molecular spring and mechanosensor and has been linked to striated muscle disease. The pathways that govern titin-dependent cardiac growth and contribute to disease are diverse and difficult to dissect. METHOD...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.118.037588
更新日期:2019-04-09 00:00:00
abstract:BACKGROUND:Left ventricular (LV) mass is an important predictor of heart failure and cardiovascular mortality, yet determinants of LV mass are incompletely understood. Pulmonary hyperinflation in chronic obstructive pulmonary disease (COPD) may contribute to changes in intrathoracic pressure that increase LV wall stres...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.113.001653
更新日期:2013-04-09 00:00:00
abstract::The Beta-Blocker Heart Attack Trial was a multicenter, randomized, double-blind, placebo-controlled trial of propranolol therapy in 3837 men and women with acute myocardial infarction. The patients began their treatment 5-21 days after hospital admission (mean 13.8 days). During an average follow-up of 25 months, ther...
journal_title:Circulation
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1983-06-01 00:00:00
abstract:BACKGROUND:Many patients with implanted cardioverter defibrillators (ICDs) receive adjunctive antiarrhythmic drug therapy, most commonly amiodarone or sotalol. The effects of these drugs on defibrillation energy requirements have not been previously assessed in a randomized controlled trial. METHODS AND RESULTS:The Op...
journal_title:Circulation
pub_type: 杂志文章,随机对照试验
doi:10.1161/CIRCULATIONAHA.106.618421
更新日期:2006-07-11 00:00:00
abstract::Six instances of sudden death were recorded by Holter monitoring, which showed ventricular fibrillation in five and a bradyarrhythmia in one. Complex ventricular ectopic activity preceded cardiac arrest in five patients, including the one with the bradyarrhythmic arrest. Two patients with chronic bifascicular block ar...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.66.1.218
更新日期:1982-07-01 00:00:00
abstract::Phasic blood flow recorded in five unobstructed dog aortocoronary bypass grafts to the left coronary artery was similar to that of normal coronary arteries, with a high diastolic-to-systolic flow ratio which averaged 2.7 +/- 0.4. With an average of 52 +/- 7% and 72 +/- 6% stenosis in the coronary artery distal to the ...
journal_title:Circulation
pub_type: 杂志文章
doi:
更新日期:1975-08-01 00:00:00
abstract:BACKGROUND:A variable degree of smooth muscle cell (SMC) proliferation after balloon injury has been reported in previous rat studies. It is unknown whether balloon injury induces c-fos expression and whether it is related to the degree of vascular injury in vivo. Therefore, we tested the hypothesis that proportional i...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.92.5.1230
更新日期:1995-09-01 00:00:00
abstract:BACKGROUND:This study proposes 2 new echocardiographic indices with potential application in acute coronary artery occlusion to differentiate between viable and necrotic myocardium and to identify reperfusion. We investigated whether the ratio between systolic lengthening and combined late and postsystolic shortening (...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.105.533372
更新日期:2005-12-20 00:00:00
abstract:BACKGROUND:Hypertension is a major modifiable cardiovascular risk factor. The present longitudinal study aimed to examine the best combination of childhood physical and environmental factors to predict adult hypertension and furthermore whether newly identified genetic variants for blood pressure increase the predictio...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.111.085977
更新日期:2012-07-24 00:00:00
abstract::An asymptomatic 14-year-old male was found at cardiac catheterization to have a coronary artery fistula involving a vessel originating from the left main coronary artery and terminating in the right heart. Chest X-ray and electrocardiogram were within normal limits and shunt flow was too small to be detected by oximet...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.57.1.197
更新日期:1978-01-01 00:00:00
abstract::In 1948 knowledge of the role of the central nervous system in controlling blood pressure was rudimentary and the possibility that the brain could contribute to hypertension was barely acknowledged. Over the past 35 years the development of new technologies, particularly those of the neurosciences, and their applicati...
journal_title:Circulation
pub_type: 历史文章,杂志文章
doi:
更新日期:1984-11-01 00:00:00
abstract:BACKGROUND:PCTP (phosphatidylcholine transfer protein) regulates the intermembrane transfer of phosphatidylcholine. Higher platelet PCTP expression is associated with increased platelet responses on activation of protease-activated receptor 4 thrombin receptors noted in black subjects compared with white subjects. Litt...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.116.023711
更新日期:2017-09-05 00:00:00
abstract:BACKGROUND:Chronic heart failure (CHF) is associated with endothelial dysfunction including impaired endothelium-mediated, flow-dependent dilation (FDD). There is evidence for increased radical formation in CHF, raising the possibility that nitric oxide is inactivated by radicals, thereby impairing endothelial function...
journal_title:Circulation
pub_type: 临床试验,杂志文章
doi:10.1161/01.cir.97.4.363
更新日期:1998-02-03 00:00:00
abstract:BACKGROUND:Interaction of complement with endothelial cells (ECs) underlies the development of inflammation and coagulation in disease. Assembly of the membrane attack complex (MAC) of complement on EC membrane, like stimulation with cytokines, upregulates tissue factor and cyclooxygenase-2 but does so via the intermed...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.101.15.1867
更新日期:2000-04-18 00:00:00
abstract:BACKGROUND:Coronary blood flow occurs mainly during the diastolic phase of each cardiac cycle and is mainly dependent on diastolic driving pressure, especially in the left anterior descending coronary artery (LAD). We hypothesized that calculation of the ratio of the diastolic driving pressure of a stenotic LAD to its ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.102.19.2365
更新日期:2000-11-07 00:00:00
abstract:BACKGROUND:Initially based on histology, the diagnosis of renal artery fibromuscular dysplasia (FMD) is now based mostly on angiographic appearance because arterial tissue samples are rarely available. This retrospective cross-sectional study aimed to assess the clinical relevance of a binary angiographic classificatio...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.112.117499
更新日期:2012-12-18 00:00:00
abstract::It is generally agreed that the conditions leading to myocardial ischemia occur when the oxygen demands of the myocardium exceed the oxygen supply. The major limitations of oxygen supply to the myocardium are atherogenic disease and vasospasm of the coronary arteries. The major hemodynamic determinants of myocardial o...
journal_title:Circulation
pub_type: 杂志文章
doi:
更新日期:1983-06-01 00:00:00
abstract:BACKGROUND:Cystic medial degeneration (CMD) is a histological abnormality that is common in the aortic diseases associated with Marfan's syndrome (MFS). Although little known about the mechanism underlying CMD, several recent reports have demonstrated that vascular smooth muscle cell (VSMC) apoptosis could play a subst...
journal_title:Circulation
pub_type: 临床试验,杂志文章
doi:10.1161/hc37t1.094856
更新日期:2001-09-18 00:00:00
abstract:BACKGROUND:Granulocyte-colony stimulating factor (G-CSF) after myocardial infarction does not affect systolic function when compared with placebo. In contrast, intracoronary infusion of bone marrow cells appears to improve ejection fraction. We aimed to evaluate the G-CSF mobilization of subsets of stem cells. METHODS...
journal_title:Circulation
pub_type: 杂志文章,随机对照试验
doi:10.1161/CIRCULATIONAHA.106.678649
更新日期:2007-09-11 00:00:00
abstract:BACKGROUND:Although patients with heart failure express elevated circulating levels of tumor necrosis factor-alpha (TNF-alpha) in their peripheral circulation, the structural and functional effects of circulating levels of pathophysiologically relevant concentrations of TNF-alpha on the heart are not known. METHODS AN...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.97.14.1382
更新日期:1998-04-14 00:00:00
abstract:BACKGROUND:An objective of the United States' Healthy People 2010 Initiative is to eliminate disparities based on socioeconomic status. We assessed the effect of difficulty affording health care on the health status (symptoms, function, and quality of life) of patients treated with percutaneous coronary intervention or...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.104.474775
更新日期:2005-05-24 00:00:00