Abstract：
:The surface expression and metabolic turnover time of alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptors are important factors in determining the involvement of these proteins in synaptic function. We used the membrane-impermeant chemical crosslinking reagent BS3 and subsequent immunoblot analysis to show that a large proportion of each of the AMPA receptor subunits GluR1-4 is surface expressed in cultured rat cerebellar granule cells. The apparent molecular mass of the individual subunits was 105 kDa and the surface expressed crosslinked complex was 530 kDa for each of the antibodies tested. These results are consistent with functional ionotropic glutamate receptors being pentameric subunit assemblies. We investigated the time course of AMPA receptor surface expression using the membrane-impermeant biotinylating agent NHS-SS-biotin. The half-life of surface expressed AMPA receptors was found to be 30 h. To estimate the mean degradation rate of AMPA receptors and total trichloracetic acid (TCA)-precipitable protein we used [35S]methionine/cysteine pulse-chase labelling. The half-life of AMPA receptors immunoprecipitated with anti-GluR1 antibody was approximately 48 h and the half-life of total TCA-precipitable protein from the same samples was 37 h.
journal_name
Neuropharmacologyjournal_title
Neuropharmacologyauthors
Archibald K,Perry MJ,Molnár E,Henley JMdoi
10.1016/s0028-3908(98)00135-xsubject
Has Abstractpub_date
1998-10-01 00:00:00pages
1345-53issue
10-11eissn
0028-3908issn
1873-7064journal_volume
37pub_type
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