Endothelial dysfunction in children with obstructive sleep apnea is associated with epigenetic changes in the eNOS gene.

Abstract:

BACKGROUND:Obstructive sleep apnea (OSA) is a highly prevalent disorder that has been associated with an increased risk for cardiovascular morbidity, even in children. However, not all children with OSA manifest alterations in endothelial postocclusive hyperemia, an endothelial nitric oxide synthase (eNOS)-dependent response. Since expression of the eNOS gene is regulated by epigenetic mechanisms and OSA may cause epigenetic modifications such as DNA hypermethylation, we hypothesized that epigenetic modifications in the eNOS gene may underlie the differential vascular phenotypes in pediatric OSA. METHODS:Age-, sex-, ethnicity-, and BMI-matched prepubertal children with polysomnographically confirmed OSA and either normal (OSAn) or abnormal (OSAab) postocclusive hyperemic responses, assessed as the time to attain peak reperfusion flow (Tmax) by laser Doppler flowmetry, were recruited. Blood genomic DNA was assessed for epigenetic modifications in the eNOS gene using pyrosequencing. Children with no evidence of OSA or endothelial dysfunction served as a control group. RESULTS:The study comprised 36 children with OSA (11 with OSAab and 25 with OSAn) and 35 children in the control group. Overall, the mean age was 7.5 ± 2.4 years, 65% were boys, and 30% were obese; mean apnea-hypopnea index was 18 ± 8.6/h of sleep for the children with OSA. Tmax was 66.7 ± 8.8 s in the OSAab group and 30.1 ± 8.3 s in the OSAn group (P < .001). Pyrosequencing of the proximal promoter region of the eNOS gene revealed no significant differences in six of the seven CpG sites. However, a CpG site located at position -171 (relative to transcription start site), approximating important transcriptional elements, displayed significantly higher methylation levels in the OSAab group as compared with the OSAn or control groups (81.5% ± 3.5%, 74.8% ± 1.4%, and 74.5% ± 1.7%, respectively; P < .001). eNOS mRNA expression levels were assessed in a separate group of children and were significantly reduced in the OSAab group in comparison with the OSAn group. CONCLUSIONS:The presence of abnormal eNOS-dependent vascular responses in children with OSA is associated with epigenetic modifications in the eNOS gene.

journal_name

Chest

journal_title

Chest

authors

Kheirandish-Gozal L,Khalyfa A,Gozal D,Bhattacharjee R,Wang Y

doi

10.1378/chest.12-2026

subject

Has Abstract

pub_date

2013-04-01 00:00:00

pages

971-977

issue

4

eissn

0012-3692

issn

1931-3543

pii

S0012-3692(13)60206-8

journal_volume

143

pub_type

杂志文章

相关文献

CHEST文献大全
  • Eosinophilic pneumonia without radiographic pulmonary infiltrates.

    abstract::In a 52-year-old man, chronic eosinophilic pneumonia (CEP), suggested by clinical history and marked eosinophilia in the peripheral blood and in the bronchoalveolar lavage fluid, has been diagnosed on open lung biopsy. Nevertheless, chest roentgenograms never showed any infiltration during the course of the disease. E...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.84.5.637

    authors: Dejaegher P,Derveaux L,Dubois P,Demedts M

    更新日期:1983-11-01 00:00:00

  • Asthma and Cushing's syndrome.

    abstract::A female patient was treated with high-dose inhaled fluticasone propionate for her asthma. Over 2 years, she developed features of Cushing's syndrome with proximal myopathy, osteopenia, hypertension, depressive psychosis, and cushingoid appearance. She had biochemical evidence of marked adrenal suppression with a 9:00...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.117.2.593

    authors: Wilson AM,Blumsohn A,Jung RT,Lipworth BJ

    更新日期:2000-02-01 00:00:00

  • Supplemental oxygen and exercise performance in patients with cystic fibrosis with severe pulmonary disease.

    abstract::Patients with cystic fibrosis (CF) and advanced pulmonary disease have pulmonary limitation of exercise, often associated with arterial oxygen desaturation. Improving oxygenation during exercise by providing supplemental oxygen may improve exercise performance in these patients. To test this, we performed graded exerc...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.101.1.52

    authors: Marcus CL,Bader D,Stabile MW,Wang CI,Osher AB,Keens TG

    更新日期:1992-01-01 00:00:00

  • Effects of carbohydrates on carbon dioxide excretion in patients with airway disease.

    abstract::Thirteen patients with stable airway disease were fed a diet high in carbohydrates, and 35 minutes later metabolic and ventilatory effects were observed. The six patients who did not retain carbon dioxide increased their mean respiratory quotient from 0.86 +/- 0.07 to 1.03 +/- 0.04, their mean minute ventilation from ...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.71.1.55

    authors: Gieseke T,Gurushanthaiah G,Glauser FL

    更新日期:1977-01-01 00:00:00

  • Periodic leg movement, nasal CPAP, and expiratory muscles.

    abstract:BACKGROUND:Periodic leg movements (PLMs) may appear during nasal CPAP titration, persisting despite the elimination of hypopneas. METHODS:Systematic recordings of expiratory abdominal muscles on the right and left sides with surface electromyographic (EMG) electrodes lateral to navel, and close from the lateral side o...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.11-1563

    authors: Seo WH,Guilleminault C

    更新日期:2012-07-01 00:00:00

  • Indications for spirometry in outpatients with respiratory disease.

    abstract::It has been suggested that spirometry should be incorporated into the routine examination of every patient, analogous to obtaining vital signs. To determine the impact of spirometry on the management of outpatients with respiratory disease, spirometry was performed on 150 consecutive patients (123 men and 27 women, me...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.99.3.730

    authors: Owens MW,Anderson WM,George RB

    更新日期:1991-03-01 00:00:00

  • Recurrent, self-limited, menstrual-associated bronchiolitis obliterans organizing pneumonia.

    abstract::A 39-year-old woman presented with recurrent acute illness, characterized by high-grade fever, pleuritic chest pain, and unilateral nodular infiltrate on chest radiograph. During the follow-up period, there were six similar episodes, each starting 2 to 3 days prior to her menstrual period and resolving within 5 to 10 ...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.118.1.253

    authors: Yigla M,Ben-Itzhak O,Solomonov A,Guralnik L,Oren I

    更新日期:2000-07-01 00:00:00

  • Exercise-induced pulmonary arterial hypertension in patients with systemic sclerosis.

    abstract:INTRODUCTION:Pulmonary arterial hypertension (PAH) is the most common cause of scleroderma-related deaths. New medications for PAH patients make it necessary to identify patients with high risk factors for PAH. This study looks at the use of an exercise echocardiogram in identifying patients who may have PAH and may be...

    journal_title:Chest

    pub_type: 临床试验,杂志文章

    doi:10.1378/chest.07-2324

    authors: Steen V,Chou M,Shanmugam V,Mathias M,Kuru T,Morrissey R

    更新日期:2008-07-01 00:00:00

  • Improved left ventricular relaxation during short-term right ventricular outflow tract compared to apical pacing.

    abstract:STUDY OBJECTIVES:Pacing-induced asynchrony may deteriorate left ventricular function; however, limited data exists in humans. The aim of our study was to compare left ventricular hemodynamics during short-term atrioventricular sequential pacing from the right ventricular apex and from the outflow tract of the right ven...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.117.1.60

    authors: Kolettis TM,Kyriakides ZS,Tsiapras D,Popov T,Paraskevaides IA,Kremastinos DT

    更新日期:2000-01-01 00:00:00

  • Intrapleural talc for the prevention of recurrent pneumothorax.

    abstract::The ipsilateral recurrence rate after the first spontaneous pneumothorax treated with tube thoracostomy is reported to be between 23 percent and 52 percent. The incidence of recurrence after the first recurrence is substantially higher. Chemical pleurodesis has been attempted to decrease the recurrence rate. Tetracycl...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.106.4.1162

    authors: Milanez JR,Vargas FS,Filomeno LT,Fernandez A,Jatene A,Light RW

    更新日期:1994-10-01 00:00:00

  • The uncommon case of Jahi McMath.

    abstract::A 13-year-old patient named Jahi McMath was determined to be dead by neurologic criteria following cardiopulmonary arrest and resuscitation at a hospital in Oakland, California. Her family did not agree that she was dead and refused to allow her ventilator to be removed. The family's attorney stated in the media that ...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.14-2227

    authors: Luce JM

    更新日期:2015-04-01 00:00:00

  • Propranolol-induced urticaria: successful therapy with tolamolol.

    abstract::Urticaria with systemic effects occurred in a patient being treated with propranolol and was reproducible upon rechallenge with the drug; Successful beta-adrenergic blocking therapy was achieved using a new agent, tolamolol, with no evidence of any adverse effects. ...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.67.4.496

    authors: Seides SF,Ruskin JN,Damato AN

    更新日期:1975-04-01 00:00:00

  • Nuclear accumulation of p53 is a potential marker for the development of squamous cell lung cancer in smokers.

    abstract:STUDY OBJECTIVES:To determine whether the nuclear accumulation of p53 in patients with early bronchial neoplasia represents an altered susceptibility for the development of lung cancer. PATIENTS AND MEASUREMENTS:We evaluated the percentage of cells accumulating nuclear p53 immunohistochemically in squamous cell carcin...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.123.1.181

    authors: Piyathilake CJ,Frost AR,Manne U,Weiss H,Heimburger DC,Grizzle WE

    更新日期:2003-01-01 00:00:00

  • Fatal acute congestive heart failure in a patient with idiopathic hemochromatosis and cocaine use.

    abstract::Cardiovascular complications from cocaine use have been recognized in increasing frequency in recent years. We report the case of a young man with a history of intranasal cocaine use presenting with acute congestive heart failure who, on postmortem examination, was found to have idiopathic hemochromatosis. It is specu...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.92.2.374

    authors: Goldenberg SP,Zeldis SM

    更新日期:1987-08-01 00:00:00

  • Low and High Blood Eosinophil Counts as Biomarkers in Hospitalized Acute Exacerbations of COPD.

    abstract:BACKGROUND:Characterizing acute exacerbations of COPD (AECOPD) and individualizing therapy is challenging. Key exacerbation therapies include antibiotics and systemic corticosteroids. Blood eosinophils, when either low or high, may offer a simple, inexpensive distinction to predict beneficial responses to these therapi...

    journal_title:Chest

    pub_type: 杂志文章,多中心研究

    doi:10.1016/j.chest.2019.02.406

    authors: MacDonald MI,Osadnik CR,Bulfin L,Hamza K,Leong P,Wong A,King PT,Bardin PG

    更新日期:2019-07-01 00:00:00

  • Nasal polyps in cystic fibrosis: clinical endoscopic study with nasal lavage fluid analysis.

    abstract:STUDY OBJECTIVES:Nasal polyps frequently appear in patients with cystic fibrosis (CF). The aims of this study were to focus on what problems (symptoms, endoscopic findings, and laboratory correlates) nasal polyps cause the CF patient, and how these correlate to the total health situation of this patient group. PATIENT...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.121.1.40

    authors: Henriksson G,Westrin KM,Karpati F,Wikström AC,Stierna P,Hjelte L

    更新日期:2002-01-01 00:00:00

  • Idiopathic bronchiolitis obliterans with organizing pneumonia. An acute and life-threatening syndrome.

    abstract::Idiopathic bronchiolitis obliterans with organizing pneumonia (BOOP) is a clinicopathologic syndrome characterized by an indolent course and favorable prognosis. This report describes five patients with a fulminating and life-threatening variant of this syndrome. Four patients presented with respiratory failure requir...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.108.1.271

    authors: Nizami IY,Kissner DG,Visscher DW,Dubaybo BA

    更新日期:1995-07-01 00:00:00

  • Acute pulmonary exacerbations of sarcoidosis.

    abstract::Exacerbations of sarcoidosis are common. In particular, exacerbations of pulmonary sarcoidosis are reported in more than one-third of patients. Despite their frequent occurrence, there is little medical evidence concerning the definition, diagnosis, and treatment of pulmonary exacerbations of sarcoidosis. In this arti...

    journal_title:Chest

    pub_type: 杂志文章,评审

    doi:10.1378/chest.12-1060

    authors: Panselinas E,Judson MA

    更新日期:2012-10-01 00:00:00

  • Cartilaginous variant of congenital adenomatoid malformation of the lung.

    abstract::We report the occurrence of an unusual variant of congenital adenomatoid malformation (CAM) of the lung in a 16-year-old male. Abundant cartilage in the walls of malformed bronchioles, an extremely rare finding in CAM, distinguishes this lesion from the usual forms. We present the roentgenographic, computed tomographi...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.92.3.514

    authors: Benning TL,Godwin JD,Roggli VL,Askin FB

    更新日期:1987-09-01 00:00:00

  • Effect of mechanical ventilation on hepatic drug pharmacokinetics.

    abstract::Mechanical ventilation was able to induce a decrease in cardiac output and regional blood flow, especially hepatic flow. Thus, hepatic elimination of drugs with a high hepatic-extraction ratio, which was linked to alteration in hepatic blood flow, could be reduced during mechanical ventilation. The aim of this work wa...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.90.6.837

    authors: Richard C,Berdeaux A,Delion F,Riou B,Rimailho A,Giudicelli JF,Auzépy P

    更新日期:1986-12-01 00:00:00

  • Humidifier lung. An outbreak in office workers.

    abstract::Symptoms consistent with hypersensitivity pneumonitis (HP) occurred in 26 of 50 employees working in a localized area of a large factory. This area was served by a single heating-cooling unit utilizing a water humidification system. The illness consisted of flu-like symptoms with fever, chills, headache, cough, dyspne...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.77.2.183

    authors: Ganier M,Lieberman P,Fink J,Lockwood DG

    更新日期:1980-02-01 00:00:00

  • Immunosuppressive therapy in connective tissue diseases-associated pulmonary arterial hypertension.

    abstract:STUDY OBJECTIVE:Immune and inflammatory mechanisms could play a significant role in pulmonary arterial hypertension (PAH) genesis or progression, especially in patients with connective tissue diseases. Immunosuppressive therapy should be better evaluated in this setting. STUDY DESIGN:Monocentric retrospective study. ...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.130.1.182

    authors: Sanchez O,Sitbon O,Jaïs X,Simonneau G,Humbert M

    更新日期:2006-07-01 00:00:00

  • The timing of tracheotomy in critically ill patients undergoing mechanical ventilation: a systematic review and meta-analysis of randomized controlled trials.

    abstract:BACKGROUND:The objective of this study was to systematically review and quantitatively synthesize all randomized controlled trials (RCTs), comparing important outcomes in ventilated critically ill patients who received an early or late tracheotomy. METHODS:A systematic literature search of PubMed, Cumulative Index to ...

    journal_title:Chest

    pub_type: 杂志文章,meta分析,评审

    doi:10.1378/chest.11-2024

    authors: Wang F,Wu Y,Bo L,Lou J,Zhu J,Chen F,Li J,Deng X

    更新日期:2011-12-01 00:00:00

  • Physical and Social Environment Relationship With Sleep Health and Disorders.

    abstract::Sleep health is a multidimensional construct that includes adequate duration, quality, and appropriately timed sleep that may be influenced by environmental factors. In this review, we focus on how an individual's living and sleeping environment, both the surrounding neighborhood physical and social features and the a...

    journal_title:Chest

    pub_type: 杂志文章,评审

    doi:10.1016/j.chest.2019.12.002

    authors: Billings ME,Hale L,Johnson DA

    更新日期:2020-05-01 00:00:00

  • Power spectral analysis of EEG activity during sleep in cigarette smokers.

    abstract:BACKGROUND:Research on the effects of cigarette smoking on sleep architecture is limited. The objective of this investigation was to examine differences in sleep EEG between smokers and nonsmokers. METHODS:Smokers and nonsmokers who were free of all medical comorbidities were matched on different factors, including ag...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.07-1190

    authors: Zhang L,Samet J,Caffo B,Bankman I,Punjabi NM

    更新日期:2008-02-01 00:00:00

  • A test of the practical value of estimating breath sound intensity. Breath sounds related to measured ventilatory function.

    abstract::Each of four examiners performed standardized physical examinations on a group of patients who had just undergone tests of ventilatory function. The intensity of breath sounds heard with deep inspiration was graded on a rating scale of 0 to 4; the grades in six areas of the chest were added to give a total score, with...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.70.3.341

    authors: Pardee NE,Martin CJ,Morgan EH

    更新日期:1976-09-01 00:00:00

  • Frequency dependent phase and amplitude differences between simulated mouth and pleural pressures during panting. Demonstration with a mechanical model.

    abstract::A mechanical model was employed to examine phase and amplitude relationships between simulated pleural and mouth pressures during panting at different frequencies with and without lower airway obstruction and with rigid or flaccid extrathoracic airways. Results confirm the hypothesis that, in the presence of lower air...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.86.4.589

    authors: Knudson RJ,Knudson DE

    更新日期:1984-10-01 00:00:00

  • Oral corticosteroids increase esophageal acid contact times in patients with stable asthma.

    abstract:STUDY OBJECTIVES:The prevalence of gastroesophageal reflux disease (GERD) is higher in people with asthma than in control populations. Predisposing factors for GERD development may include asthma medications such as prednisone. The objective of this study was to determine whether prednisone alters GERD parameters in pe...

    journal_title:Chest

    pub_type: 临床试验,杂志文章

    doi:10.1378/chest.121.2.625

    authors: Lazenby JP,Guzzo MR,Harding SM,Patterson PE,Johnson LF,Bradley LA

    更新日期:2002-02-01 00:00:00

  • Endobronchial tuberculosis simulating lung cancer.

    abstract::Endobronchial tuberculosis, while relatively common before the advent of effective antituberculosis chemotherapy, is seldom recognized now. Four patients with what appeared to be primary tuberculosis complicated by endobronchial spread were recently seen by us. Three of the four patients presented with anterior segmen...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.86.4.642

    authors: Matthews JI,Matarese SL,Carpenter JL

    更新日期:1984-10-01 00:00:00

  • Efficacy of positive vs negative pressure ventilation in unloading the respiratory muscles.

    abstract::We compared the efficacy of positive pressure ventilation (PPV) vs negative pressure ventilation (NPV) in providing ventilatory muscle rest for five normal subjects and six patients with chronic obstructive pulmonary disease (COPD). All participants underwent measurement of transdiaphragmatic pressure (Pdi), pressure ...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.98.4.850

    authors: Belman MJ,Soo Hoo GW,Kuei JH,Shadmehr R

    更新日期:1990-10-01 00:00:00