Double paths of macronucleus differentiation at conjugation in Blepharisma japonicum.

Abstract:

:Nuclear phenomena at conjugation of the multimicronucleate ciliate Blepharisma japonicum were investigated by using fluorescence microscopy. Several hours after cells united in pairs, micronuclei differentiated into meiotic micronuclei and "somatomicronuclei". Meiotic micro-nuclei participated in conventional sexual processes producing new micro- and macronuclei through meiosis, karyogamy and postkaryogamic mitoses. On the other hand, somatomicronuclei participated in characteristic asexual processes in which each micronucleus differentiated into a macronucleus without undergoing any nuclear division and karyogamy. These two paths of macronucleus differentiation, one sexual and the other asexual, proceeded side by side in each cell, but eventually one path dominated the other. The sexual path regularly dominated in crossing conjugation which was induced by mixing clones of complementary mating types I and II. However, if macronuclear anlagen in the sexual path were removed, the asexual path took over to form macronuclei, indicating that the asexual path serves as a reserve path of macronucleus formation. The asexual path regularly dominated in intraclonal conjugation of high-frequency selfers, suggesting that it functions in reducing the genetic effect of inbreeding. These findings not only clarify nuclear phenomena at conjugation in Blepharisma, but also provide a new opportunity for the study of macronucleus differentiation.

journal_name

Eur J Protistol

authors

Miyake A,Rivola V,Harumoto T

doi

10.1016/S0932-4739(11)80340-8

subject

Has Abstract

pub_date

1991-06-21 00:00:00

pages

178-200

issue

2

eissn

0932-4739

issn

1618-0429

pii

S0932-4739(11)80340-8

journal_volume

27

pub_type

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