Abstract:
:A new species of unicellular opisthokont protist, Nuclearia thermophila sp. nov., was isolated from the warm spring water of Yunoko Lake, Japan, and has been described using light and electron microscopy. It exists as a spherical floating form and a flattened amoeboid form showing various shapes. The cells occasionally extended as branches or knobbed filopodia. The spherical form when suspended in medium measured 20-40microm in diameter (excluding filopodia). The amoeboid form may exceed 65microm along the longest axis. A nucleus with an obvious spherical nucleolus, dictyosomes, mitochondria with flat cristae, food vacuoles, and lipid droplet-like vacuoles with homogeneous contents were observed; no extracellular matrix or bacterial endosymbionts were present. The cells ingested flour particles. No cysts were seen. The molecular phylogenetic tree constructed on the basis of the small subunit ribosomal DNA revealed the novelty of N. thermophila and its relationships with previously known nucleariids.
journal_name
Eur J Protistoljournal_title
European journal of protistologyauthors
Yoshida M,Nakayama T,Inouye Idoi
10.1016/j.ejop.2008.09.004subject
Has Abstractpub_date
2009-05-01 00:00:00pages
147-55issue
2eissn
0932-4739issn
1618-0429pii
S0932-4739(08)00080-1journal_volume
45pub_type
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