Abstract：
:Poly(vinyl acetate-acrylamide) microspheres were synthesized in the absence or presence of isooctane via suspension polymerization and utilized as carriers to immobilize Candida rugosa lipase. When the hydrophobic/hydrophilic surface characteristics of the microspheres were modified by changing the ratio of vinyl acetate (hydrophobic monomer) to acrylamide (hydrophilic monomer) from 50:50 to 86:24, the immobilization ratio changed from 45% to 92% and the activity of the immobilized lipase increased from 202.5 to 598.0 U/g microsphere. Excessive lipase loading caused intermolecular steric hindrance, which resulted in a decline in lipase activity. The maximum specific activity of the immobilized lipase (4.65 U/mg lipase) was higher than that of free lipase (3.00 U/mg lipase), indicating a high activity recovery during immobilization.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Zhang DH,Yuwen LX,Li C,Li YQdoi
10.1016/j.biortech.2012.08.083subject
Has Abstractpub_date
2012-11-01 00:00:00pages
233-6eissn
0960-8524issn
1873-2976pii
S0960-8524(12)01277-1journal_volume
124pub_type
杂志文章abstract：:A loop microbial fuel cell (MFC) system consisting of a denitrifying organics/sulfide removal MFC and a nitrification chamber was developed, and its performance at different feeding ratios of total organic carbon to sulfide (TOC/S) and dissolved oxygen (DO) levels of cathodic feeding were investigated. High feeding TO...
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