Abstract:
:Osthole, a main active constituent from Cnidium monnieri (L.) CUSSON, has been considered therapeutic agent in the treatment of ischemic stroke. This study was designed to investigate the effect of osthole on permanent middle cerebral artery occlusion (MCAO) in rats. Osthole was administrated by gavage to the normal and the MCAO rats. Rats were assessed for neurological deficit after 24 h following MCAO, then their brains were evaluated to determine the infarct area, and the mRNA and protein levels of some inflammatory factors were detected. It was found that MCAO animals pre-treated with osthole for 7 d showed significant improvement in all neurological tests compared with vehicle-treated MCAO groups. In addition, there was a significant decrease in infarct volume 24 h after occlusion in animals pre-treated with osthole versus the vehicle-treated MCAO group. MCAO also dramatically caused some inflammatory factors increase. However, pretreatment with osthole restored the mRNA and protein levels of these factors, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) of ischemic penumbra cortices, suggesting that osthole possessed the function of preventing brain against ischemic damage, while no significant difference was found in any of normal groups with or without osthole. The present study demonstrated that osthole may be a novel neuroprotective therapy in the treatment of focal ischemic stroke.
journal_name
Biol Pharm Bulljournal_title
Biological & pharmaceutical bulletinauthors
Li F,Gong Q,Wang L,Shi Jdoi
10.1248/bpb.b12-00133subject
Has Abstractpub_date
2012-01-01 00:00:00pages
1686-90issue
10eissn
0918-6158issn
1347-5215pii
DN/JST.JSTAGE/bpb/b12-00133journal_volume
35pub_type
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