Abstract:
:Umbilical cord blood (UCB) is used for HSCT. It is known that UCB can comprise Ag-specific T cells. Here we question whether solely transmaternal cell flow may immunize UCB. Twenty-three female UCB samples were collected from healthy mothers and analyzed for minor histocompatibility Ag HY-specific responses. Forty-two of 104 tetramer(pos) T-cell clones, isolated from 16 of 17 UCB samples, showed male-specific lysis in vitro. Male microchimerism was present in 6 of 12 UCB samples analyzed. In conclusion, female UCB comprises HY-specific cytotoxic T cells. The immunization is presumably caused by transmaternal cell flow of male microchimerism present in the mother. The presence of immune cells in UCB that are not directed against maternal foreign Ags is remarkable and may explain the reported clinical observation of improved HSCT outcome with younger sibling donors.
journal_name
Bloodjournal_title
Bloodauthors
Dierselhuis MP,Blokland EC,Pool J,Schrama E,Scherjon SA,Goulmy Edoi
10.1182/blood-2012-02-410571subject
Has Abstractpub_date
2012-07-19 00:00:00pages
505-10issue
3eissn
0006-4971issn
1528-0020pii
blood-2012-02-410571journal_volume
120pub_type
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