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Characterization of a methane-oxidizing biofilm using microarray, and confocal microscopy with image and geostatic analyses.

Abstract：

:A mixed methane-oxidizing biofilm was characterized, concurrently using a number of advanced techniques. Community analysis results by microarray exhibited that type II members dominated the methanotrophic community, in which Methylocystis was most abundant, followed by Methylosinus. Observation results by fluorescent in situ hybridization and confocal microscopy showed multiple biofilm colonies that were irregular, bell-shaped, with mean thickness of approximately 20 μm. Image analysis results indicated that the relative abundance of methanotrophs peaked at a depth of about 5 μm. Although the biofilm colonies differed in size, methanotrophs accounted for 4-9%. Gaussian and linear regression results between the biofilm volumes and types I (r (2) = 0.86) and II volumes (r (2) = 0.92), respectively, revealed that type I members played a role in the growth of the biofilm but only below a threshold volume, whereas type II members supported the overall growth. Geostatistical analyses results revealed concentration of types I and II methanotrophic individuals with decreasing depth, and randomness between the spatial locations and population levels. Collectively, the methane-oxidizing biofilm was a highly organized system with methanotrophs and their cohabitants.

journal_name

Appl Microbiol Biotechnol

journal_title

Applied microbiology and biotechnology

authors

Kim TG,Yi T,Lee EH,Ryu HW,Cho KS

doi

10.1007/s00253-011-3728-y

subject

Has Abstract

pub_date

2012-08-01 00:00:00

pages

1051-9

issue

4

eissn

0175-7598

issn

1432-0614

journal_volume

95

pub_type

杂志文章

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