Abstract:
BACKGROUND:In this study, we have investigated the chemotherapeutic potential of a purple violet pigment (PVP), which was isolated from a previously undescribed Antarctic Janthinobacterium sp. (Ant5-2), against murine UV-induced 2237 fibrosarcoma and B16F10 melanoma cells. METHODS:The 2237, B16F10, C50, and NIH3T3 cells were treated with PVP at different doses and for different times, and their proliferation and viability were detected by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle arrest induced by PVP in 2237 fibrosarcoma cells was assessed by flow cytometry and expression analysis of cell cycle regulatory proteins were done by Western blot. Apoptosis induced by PVP in 2237 cells was observed by annexin-V/propidium iodide double staining flow cytometry assay and fluorescence microscopy. To further determine the molecular mechanism of apoptosis induced by PVP, the changes in expression of Bcl-2, Bax and cytochrome c were detected by Western blot. The loss of mitochondrial membrane potential in PVP treated 2237 cells was assessed by staining with JC-1 dye following flow cytometry. Caspase-3, Caspase-9 and PARP cleavage were analyzed by Western blot and Caspase-3 and -9 activities were measured by colorimetric assays. RESULTS:In vitro treatment of murine 2237 cells with the PVP resulted in decreased cell viability (13-79%) in a time (24-72 h) and dose (0.1-1 μM)-dependent manner. The PVP-induced growth inhibition in 2237 cells was associated with both G0/G1 and G2/M phase arrest accompanied with decrease in the expression of cyclin dependent kinases (Cdks) and simultaneous increase in the expression of cyclin dependent kinase inhibitors (Cdki) - Cip1/p21 and Kip1/p27. Further, we observed a significant increase in the apoptosis of the 2237 fibrosarcoma cells which was associated with an increased expression of pro-apoptotic protein Bax, decreased expression of anti-apoptotic proteins Bcl-2, disruption of mitochondrial membrane potential, cytochrome c release, activation of caspase-3, caspase-9 and poly-ADP-ribose-polymerase (PARP) cleavage. CONCLUSIONS:We describe the anti-cancer mechanism of the PVP for the first time from an Antarctic bacterium and suggest that the PVP could be used as a potent chemotherapeutic agent against nonmelanoma skin cancers.
journal_name
Int J Dermatoljournal_title
International journal of dermatologyauthors
Mojib N,Nasti TH,Andersen DT,Attigada VR,Hoover RB,Yusuf N,Bej AKdoi
10.1111/j.1365-4632.2010.04825.xsubject
Has Abstractpub_date
2011-10-01 00:00:00pages
1223-33issue
10eissn
0011-9059issn
1365-4632journal_volume
50pub_type
杂志文章abstract:BACKGROUND:The hair cycle is regulated by various molecules, among which FGF-5 has been shown to promote the transition from anagen to catagen. The FGFR-1, a trans-membrane receptor of FGF-5 with tyrosine kinase activity, is localized in the follicular papilla of hair follicles. OBJECTIVES:In order to apply the antise...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1111/j.1365-4632.2007.03018.x
更新日期:2007-03-01 00:00:00
abstract:BACKGROUND:The aim was to examine cadexomer iodine paste in a comparative clinical trial. METHODS:A 12-week, randomized, open, controlled, multicenter, multinational trial in patients with exudating, venous leg ulcers of cadexomer iodine paste (Iodosorb/Iodoflex), hydrocolloid dressing (Duoderm E, Granuflex E), or par...
journal_title:International journal of dermatology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1046/j.1365-4362.1998.00415.x
更新日期:1998-05-01 00:00:00
abstract::Peripheral blood lymphocyte assays using monoclonal antibodies were done in 66 patients with leprosy, consisting of 25 children and 41 adults. The results were statistically analyzed for correlations, if any, among the different age groups and matched controls. The results, however, failed to show any significant corr...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1111/j.1365-4362.1991.tb04786.x
更新日期:1991-11-01 00:00:00
abstract::A clinical trial using tretinoin lotion 0.05%, benzoyl peroxide 5% and 10% as topical application was performed on 250 ambulatory patients suffering from various degrees of acne vulgaris. The results indicate that tretinoin applied in the morning and benzoyl peroxide applied at night is the most efficacious regimen to...
journal_title:International journal of dermatology
pub_type: 临床试验,杂志文章
doi:10.1111/j.1365-4362.1979.tb01956.x
更新日期:1979-07-01 00:00:00
abstract:BACKGROUND:Hidradenitis suppurativa (HS) is a chronic inflammatory disease involving the skin bearing apocrine glands. There are numerous comorbidities and associated diseases among patients with HS. The association of HS and thyroid abnormalities is equivocal. We aimed to explore whether HS is associated with thyroid ...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1111/ijd.15319
更新日期:2020-11-25 00:00:00
abstract::Pruritus is among the most common complaints in the field of dermatology. It is also a disturbing symptom of many systemic disorders. Chronic pruritus (CP) refers to the cases of the symptom which last longer than 6 weeks. We conducted a prospective analysis of patients with generalized pruritus without primary skin l...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1111/ijd.14125
更新日期:2019-03-01 00:00:00
abstract:BACKGROUND:There is little research available that describes the natural progression of split-thickness skin graft (STSG) donor site wounds after re-epithelialization. The purpose of this study was to evaluate the longitudinal clinical progress of transepidermal water loss (TEWL), color, and sensory elements in STSG do...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1111/ijd.14389
更新日期:2019-05-01 00:00:00
abstract:BACKGROUND:Psoriasis presents many management complexities. A cornerstone of therapy has been topical corticosteroids, although over the past 10 years there have been many additions to the medication armamentarium. Furthermore, various combination regimens and approaches have been advocated. OBJECTIVE:We sought to cha...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1111/j.1365-4632.2006.02532.x
更新日期:2006-04-01 00:00:00
abstract::Trimethylpsoralen, psoralen and 8-methoxypsoralen were adminstered in 10 mg dosage orally to 37, 29 and 23 patients with vitiligo. Complete improvement was noted in 8.9% and partial improvement was recorded in 59.5%. The overall results produced by trimethylpsoralen and psoralen were superior to those of 8-methoxyps...
journal_title:International journal of dermatology
pub_type: 临床试验,杂志文章
doi:10.1111/ijd.1975.14.3.205
更新日期:1975-04-01 00:00:00
abstract:BACKGROUND:An allogeneic human skin graft is a temporary biologic dressing used in extensive burns that can be a providential treatment for affected patients. Skin quality depends directly on its microbial decontamination after processing in a tissue bank. Our objective was to describe the skin donor profiles in relati...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1111/ijd.14254
更新日期:2019-03-01 00:00:00
abstract:BACKGROUND:Deep dermal and full-thickness burns are not only difficult to treat, but they are also associated with significant morbidity and mortality. Recent reports have proposed the use of mesenchymal stromal cells (MSCs) for inducing tissue repair in burn injuries. OBJECTIVE:We aim to evaluate the effect of alloge...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1111/ijd.14949
更新日期:2020-08-01 00:00:00
abstract:INTRODUCTION:Even though androgenetic alopecia (AGA) is the most common type of alopecia, factors associated with AGA severity have been poorly investigated. OBJECTIVES:The objective of our study was to investigate risk factors for AGA severity among a Caucasian population. METHODS:A cross-sectional study was conduct...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1111/ijd.13652
更新日期:2017-08-01 00:00:00
abstract::Demodecidosis is an ectoparasitosis that is infrequently observed in subjects with human immunodeficiency virus (HIV) infection. It has a rosacea-like presentation. We present three cases in patients with acquired immunodeficiency syndrome (AIDS). Two of these patients had a profuse eruption, and all three cases were ...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1111/j.1365-4632.2006.02924.x
更新日期:2006-09-01 00:00:00
abstract::Actinic granuloma is a rare skin disorder that develops in an area of actinic elastosis. The pathogenesis of the disease is obscure but the most accepted hypothesis implicates the solar radiation as the triggering factor. Typically the disease presents in middle-aged individuals with significant past sun-exposure and ...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1111/j.1365-4632.2005.02043.x
更新日期:2005-02-01 00:00:00
abstract:BACKGROUND:Khite (also known as fatlah in Egypt) is a manual technique of temporary vellus hair removal that is widely performed in the Middle East. METHODS:The technique of khite is reviewed as illustrated. RESULTS:Khite is an effective method of removing unwanted vellus hairs. CONCLUSIONS:Dermatologists in all par...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1046/j.1365-4362.1997.00189.x
更新日期:1997-03-01 00:00:00
abstract::A variety of cutaneous manifestations has been described in multiple myeloma including extramedullary cutaneous plasmacytomas, cutaneous amyloidosis, pyoderma gangrenosum, leukocytoclastic vasculitis, necrobiotic xanthogranuloma, scleromyxedema, Sweet's syndrome, subcorneal pustular dermatosis, scleredema, and plane x...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1111/j.1365-4632.2004.02414.x
更新日期:2006-05-01 00:00:00
abstract:BACKGROUND:Erythroderma is a generalized erythema of the skin accompanied by a variable degree of scaling. However, most of the published series originate from Western countries. There is only one report from Africa, conducted in Dakar in 1979. The aim of our study was to provide information about this condition in our...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1111/j.1365-4632.2004.02100.x
更新日期:2005-09-01 00:00:00
abstract::Pellagra secondary to antituberculous chemotherapy can result from isoniazide therapy, as well as from pyrazinamide treatment. Because of the similarity in structural formulas between pyrazinamide, isoniazide, and nicotinamide, the substrate competition is the mechanism most likely involved. The patient was cured by a...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1111/j.1365-4362.1983.tb02114.x
更新日期:1983-01-01 00:00:00
abstract:BACKGROUND:Assessment of severity is essential in the clinical care of rosacea patients and in the research on rosacea. OBJECTIVE:To determine the range of methods used to assess rosacea severity in clinical trials. METHODS:The medical literature from 1965 through 2001 was searched for rosacea clinical trials using M...
journal_title:International journal of dermatology
pub_type: 杂志文章,评审
doi:10.1046/j.1365-4362.2003.01780.x
更新日期:2003-06-01 00:00:00
abstract:BACKGROUND:A study was conducted to determine the chief agents of superficial mycoses in Malta. Data were collected over a 5-year period from mycologic investigations carried out on all dermatologic specimens sent to the Mycology Laboratory at St. Luke's Hospital in Malta. METHODS:In the period between January 1995 an...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1046/j.1365-4362.2003.01789.x
更新日期:2003-04-01 00:00:00
abstract:BACKGROUND:This is the first epidemiologic study of pemphigus in the southern region of Saudi Arabia. METHODS:Data were collected from patient records in the outpatient and inpatient departments of Asir Central Hospital during the period 1990-99. The diagnosis of pemphigus was based on the clinical, histopathologic, a...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1046/j.1365-4362.2001.01247.x
更新日期:2001-09-01 00:00:00
abstract::An 11-month-old male infant was referred to our clinic because of a painful ulcer of approximately 5 months' duration on the ventral surface of the tongue (Fig. 1). On physical examination, the lesion was circular (3 cm x 2 cm) with erythematous, raised, and indurated borders. No pathologic findings emerged from the l...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1111/j.1365-4632.2004.02554.x
更新日期:2006-09-01 00:00:00
abstract:BACKGROUND:With the increasing incidence of human immunodeficiency virus (HIV) infection and immunosuppressive therapy, the incidence of syphilis has been increasing. Given the fact that the above conditions may mask or obscure the usual clinical signs and symptoms of syphilis, a means of enhanced detection is essentia...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1046/j.1365-4362.2000.00029.x
更新日期:2000-08-01 00:00:00
abstract::There is a lack of validated tools to measure fatigue in patients with inflammatory skin, neuropsychiatric, and medical disorders. The use of nonvalidated tools may compromise the quality of data. The purpose of this meta-review was to evaluate existing fatigue scales commonly used to assess fatigue in other inflammat...
journal_title:International journal of dermatology
pub_type: 杂志文章,评审
doi:10.1111/ijd.15341
更新日期:2020-12-10 00:00:00
abstract::Hydroxyurea is a hydroxylated derivate of urea commonly used in the treatment of various hematologic disorders. Cutaneous side-effects such as alopecia, diffuse hyperpigmentation, scaling, poikiloderma, atrophy of the skin and subcutaneous tissues or nail changes can develop after long-term treatment with hydroxyurea....
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1111/j.1365-4632.2004.02450.x
更新日期:2006-02-01 00:00:00
abstract:BACKGROUND:Epidermal autografting has been used to treat vitiligo. The pigmentation achieved at the recipient site can be variegated and incomplete compared with that of the surrounding normal skin, and sometimes remains that way for a fairly long time. OBJECTIVE:We investigated whether the clinical results from epide...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1046/j.1365-4362.1998.00437.x
更新日期:1998-07-01 00:00:00
abstract:BACKGROUND:β-Cyclodextrin (βCD) xerogel containing silk fibroin (SF) was prepared, and the efficacy of the xerogel for a pressure sore developed on the back of an ICR mouse was investigated. MATERIALS AND METHODS:Tosyl βCD was conjugated to polyethyleneimine (PEI), and the βCD residues were cross-linked in an aqueous ...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1111/j.1365-4632.2011.05389.x
更新日期:2012-08-01 00:00:00
abstract::Cutaneous fibrinolytic activity was found to be increased in a patient with aquagenic pruritus both before and after contact with water. This could explain the lack of wheal formation in this affection, which is characterized by increased histamine release. ...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1111/j.1365-4362.1984.tb05665.x
更新日期:1984-01-01 00:00:00
abstract:UNLABELLED:BACKGROUND> An unchecked proliferation of fibrous tissue after an injury to the skin is the basic mechanism for keloid formation. Recent studies have shown the inhibitory effect of interferons on dermal fibroblast growth and/or collagen production. Various therapeutic modalities have been used for the treatm...
journal_title:International journal of dermatology
pub_type: 临床试验,杂志文章
doi:10.1111/j.1365-4362.1996.tb01671.x
更新日期:1996-07-01 00:00:00
abstract:OBJECTIVE:This study analyzes the major clinical characteristics of patients with active leprosy in relation to the in vitro immune response to the T-lymphocyte activator anti-CD3. METHODS:Thirty-eight patients with an established diagnosis of leprosy were classified according to the Ridley and Jopling table. Peripher...
journal_title:International journal of dermatology
pub_type: 杂志文章
doi:10.1111/j.1365-4362.1996.tb02974.x
更新日期:1996-11-01 00:00:00