The antiproliferative function of violacein-like purple violet pigment (PVP) from an Antarctic Janthinobacterium sp. Ant5-2 in UV-induced 2237 fibrosarcoma.


BACKGROUND:In this study, we have investigated the chemotherapeutic potential of a purple violet pigment (PVP), which was isolated from a previously undescribed Antarctic Janthinobacterium sp. (Ant5-2), against murine UV-induced 2237 fibrosarcoma and B16F10 melanoma cells. METHODS:The 2237, B16F10, C50, and NIH3T3 cells were treated with PVP at different doses and for different times, and their proliferation and viability were detected by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle arrest induced by PVP in 2237 fibrosarcoma cells was assessed by flow cytometry and expression analysis of cell cycle regulatory proteins were done by Western blot. Apoptosis induced by PVP in 2237 cells was observed by annexin-V/propidium iodide double staining flow cytometry assay and fluorescence microscopy. To further determine the molecular mechanism of apoptosis induced by PVP, the changes in expression of Bcl-2, Bax and cytochrome c were detected by Western blot. The loss of mitochondrial membrane potential in PVP treated 2237 cells was assessed by staining with JC-1 dye following flow cytometry. Caspase-3, Caspase-9 and PARP cleavage were analyzed by Western blot and Caspase-3 and -9 activities were measured by colorimetric assays. RESULTS:In vitro treatment of murine 2237 cells with the PVP resulted in decreased cell viability (13-79%) in a time (24-72 h) and dose (0.1-1 μM)-dependent manner. The PVP-induced growth inhibition in 2237 cells was associated with both G0/G1 and G2/M phase arrest accompanied with decrease in the expression of cyclin dependent kinases (Cdks) and simultaneous increase in the expression of cyclin dependent kinase inhibitors (Cdki) - Cip1/p21 and Kip1/p27. Further, we observed a significant increase in the apoptosis of the 2237 fibrosarcoma cells which was associated with an increased expression of pro-apoptotic protein Bax, decreased expression of anti-apoptotic proteins Bcl-2, disruption of mitochondrial membrane potential, cytochrome c release, activation of caspase-3, caspase-9 and poly-ADP-ribose-polymerase (PARP) cleavage. CONCLUSIONS:We describe the anti-cancer mechanism of the PVP for the first time from an Antarctic bacterium and suggest that the PVP could be used as a potent chemotherapeutic agent against nonmelanoma skin cancers.


Int J Dermatol


Mojib N,Nasti TH,Andersen DT,Attigada VR,Hoover RB,Yusuf N,Bej AK




Has Abstract


2011-10-01 00:00:00












  • Basal cell carcinoma in five albino Africans from the south-eastern equatorial rain forest of Nigeria.

    abstract:BACKGROUND:Basal cell carcinoma (BCC) is the most common cutaneous malignancy in the white population and occurs infrequently in dark-pigmented individuals. Albinism is an established risk factor for skin cancer in black Africans. METHODS:We evaluated the clinical histories and treatment outcomes of patients with a hi...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Asuquo ME,Agweye P,Ugare G,Ebughe G

    更新日期:2007-07-01 00:00:00

  • The combination of overweight and smoking increases the severity of androgenetic alopecia.

    abstract:INTRODUCTION:Even though androgenetic alopecia (AGA) is the most common type of alopecia, factors associated with AGA severity have been poorly investigated. OBJECTIVES:The objective of our study was to investigate risk factors for AGA severity among a Caucasian population. METHODS:A cross-sectional study was conduct...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Fortes C,Mastroeni S,Mannooranparampil TJ,Ribuffo M

    更新日期:2017-08-01 00:00:00

  • Bullous pemphigoid. Occurrence in a patient with mycosis fungoides receiving PUVA and topical nitrogen mustard therapy.

    abstract::A 57-year-old woman with mycosis fungoides developed blisters within cutaneous plaques while receiving PUVA therapy and topical nitrogen mustard. Direct and indirect immunofluorescence studies showed the findings of bullous pemphigoid. Her bullous disease was controlled after cessation of these therapies and instituti...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Patterson JW,Ali M,Murray JC,Hazra TA

    更新日期:1985-04-01 00:00:00

  • Expression of p16 protein in acral lentiginous melanoma.

    abstract:BACKGROUND:Acral lentiginous melanoma (ALM) is a clinicopathologic subtype of cutaneous malignant melanoma. ALM is the most common type of melanoma amongst Asians, Africans, and patients with mixed ancestry. In Brazil, ALM comprises 10% of cutaneous melanoma. ALM develops on palmar, plantar, and subungual skin, and its...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Hsieh R,Firmiano A,Sotto MN

    更新日期:2009-12-01 00:00:00

  • Failure of interferon-alpha 2b in the treatment of mature keloids.

    abstract:UNLABELLED:BACKGROUND> An unchecked proliferation of fibrous tissue after an injury to the skin is the basic mechanism for keloid formation. Recent studies have shown the inhibitory effect of interferons on dermal fibroblast growth and/or collagen production. Various therapeutic modalities have been used for the treatm...

    journal_title:International journal of dermatology

    pub_type: 临床试验,杂志文章


    authors: al-Khawajah MM

    更新日期:1996-07-01 00:00:00

  • Mycetoma in South India: retrospective analysis of 13 cases and description of two cases caused by unusual pathogens: Neoscytalidium dimidiatum and Aspergillus flavus.

    abstract:BACKGROUND:Mycetoma is a chronic suppurative and/or granulomatous inflammatory lesion of skin, subcutaneous tissue, fascia, and tendons caused by the traumatic inoculation of either fungal (eumycotic) or bacterial (actinomycotic) organisms present in the soil. The disease is characterized by triad of tumefaction, disch...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Padhi S,Uppin SG,Uppin MS,Umabala P,Challa S,Laxmi V,Prasad VB

    更新日期:2010-11-01 00:00:00

  • A rare case of leishmaniasis recidiva cutis evolving for 31 years caused by Leishmania tropica.

    abstract::A 64-year-old woman presented with erythematous, infiltrative plaques with a central atrophic area on both zygomatic regions. Several yellow-reddish papules were seen at the periphery of the plaques and showed an "apple-jelly" color on diascopy (Fig. 1). No visceral involvement was detected. The past medical history r...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Stefanidou MP,Antoniou M,Koutsopoulos AV,Neofytou YT,Krasagakis K,Krüger-Krasagakis S,Tselentis Y,Tosca AD

    更新日期:2008-06-01 00:00:00

  • Sézary cell count in exfoliative dermatitis.

    abstract::Sézary cells were present in a number of less than 1000/mm3 in 52 of 78 cases of various benign diffuse dermatoses and mycosis fungoides. The count was higher than 1000/mm3 in two cases of Sézary syndrome in which the histopathology was that of mycosis fungoides and in six cases of exfoliative erythroderma of undeterm...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Duangurai K,Piamphongsant T,Himmungnan T

    更新日期:1988-05-01 00:00:00

  • Jaipur block in postherpetic neuralgia.

    abstract:BACKGROUND:Postherpetic neuralgia, a common sequele to herpes zoster infection, is a chronic debilitating problem. The available therapeutic modalities are usually ineffective. METHODS:A total of 3960 patients (1326 women and 2634 men; age group, 21-84 years), with postherpetic neuralgia as the presenting complaint an...

    journal_title:International journal of dermatology

    pub_type: 临床试验,杂志文章


    authors: Bhargava R,Bhargava S,Haldia KN,Bhargava P

    更新日期:1998-06-01 00:00:00

  • Global perspectives for the management of onychomycosis.

    abstract::Onychomycosis is a fungal nail infection caused by dermatophytes, nondermatophyte molds, and yeasts. This difficult-to-treat chronic infection has a tendency to relapse despite treatment. This paper aims to offer a global perspective on onychomycosis management from expert physicians from around the world. Overall, th...

    journal_title:International journal of dermatology

    pub_type: 杂志文章,评审


    authors: Gupta AK,Mays RR,Versteeg SG,Piraccini BM,Takwale A,Shemer A,Babaev M,Grover C,Di Chiacchio NG,Taborda PRO,Taborda VBA,Shear NH,Piguet V,Tosti A

    更新日期:2019-10-01 00:00:00

  • Co-infection of mucosal leishmaniasis and extra pulmonary tuberculosis in a patient with inherent immune deficiency.

    abstract:BACKGROUND:In Sri Lanka, cutaneous leishmaniasis is a well-established disease caused by Leishmania donovani. Only a few cases of visceral disease and mucosal localization have been reported to date. CASE REPORT:A 52-year-old man presented with severe local destruction of his upper and lower lip and total destruction ...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Rathnayake D,Ranawake RR,Sirimanna G,Siriwardhane Y,Karunaweera N,De Silva R

    更新日期:2010-05-01 00:00:00

  • Considerable variation among Iranian dermatologists in the dosing and monitoring of methotrexate for treating psoriasis.

    abstract:BACKGROUND:Methotrexate (MTX) is a risky medication requiring careful attention to dosing and monitoring. Dosing and monitoring practices are not well characterized. AIMS:The aim of this study was to assess variation in the dosing and monitoring of methotrexate among Iranian dermatologists. METHODS:A questionnaire wa...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Zargari O,Hejazi S,Shahidi-Dadras M,Younespour S,Robati R,Firooz A,Toosi P,Feldman SR

    更新日期:2014-03-01 00:00:00

  • Clinical and dermoscopic patterns of melanocytic nevi in Hispanic adolescents: a descriptive study.

    abstract:BACKGROUND:Melanocytic nevi are well-known, important precursors of melanoma among children and adults. The adolescence period is an important period for nevi formation and evolution. This study provides data of a longitudinal study of nevi in a Hispanic adolescent population. MATERIALS AND METHODS:A cross-sectional s...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Sosa-Seda IM,Valentín-Nogueras S,Figueroa LD,Sánchez JL,Mercado R

    更新日期:2014-03-01 00:00:00

  • Cephalic pyoderma gangrenosum (PG)-like lesions as a presenting sign of Wegener's granulomatosis.

    abstract:BACKGROUND:Pyoderma gangrenosum (PG)-like lesions can be associated with Wegener's granulomatosis (WG); in rare instances, they could be the presenting sign of WG. The purpose of this study was to elucidate the relationship between these two disorders. METHODS:The clinical and histologic data from a case of WG with pr...

    journal_title:International journal of dermatology

    pub_type: 杂志文章,评审


    authors: Micali G,Cook B,Ronan S,Yadgir J,Solomon LM

    更新日期:1994-07-01 00:00:00

  • Treatment of impetigo and ecthyma. A comparison of sulconazole with miconazole.

    abstract::In a randomized, double-blind, parallel comparative study of 80 patients, impetigo and ecthyma were treated effectively by sulconazole nitrate 1% cream and miconazole nitrate 2% cream applied to lesions twice daily for 14 days. When treatment began, bacterial cultures from all pyodermal lesions yielded Group A beta-he...

    journal_title:International journal of dermatology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Nolting S,Strauss WB

    更新日期:1988-12-01 00:00:00

  • Relapsing polychondritis, an underestimated dermatological urgency: case report and literature review.

    abstract:BACKGROUND:Relapsing polychondritis is an autoimmune multisystemic disease with primary chondral involvement. Its high mortality and morbidity make it a real clinical challenge. CASE DESCRIPTION:A 32-year-old woman with a history of relapsing polychondritis, refractory to multiple treatments, with multisystem compromi...

    journal_title:International journal of dermatology

    pub_type: 杂志文章,评审


    authors: Cuestas D,Peñaranda E,Mora S,Cortes C,Galvis I,Patiño M,Velasquez O

    更新日期:2017-12-01 00:00:00

  • Increased cutaneous fibrinolytic activity in a case of aquagenic pruritus.

    abstract::Cutaneous fibrinolytic activity was found to be increased in a patient with aquagenic pruritus both before and after contact with water. This could explain the lack of wheal formation in this affection, which is characterized by increased histamine release. ...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Lotti T,Cappugi P,Lattari P,Panconesi E

    更新日期:1984-01-01 00:00:00

  • Topical zinc therapy for acne vulgaris.

    abstract::A double-blind study of 30 patients with mild to moderate acne vulgaris was conducted to evaluate the efficacy of a topically applied 2% zinc sulfate solution for acne therapy. Over a 12-week period, no difference was noted between placebo- and zinc-treated participants in regard to either the number or type of acne l...

    journal_title:International journal of dermatology

    pub_type: 临床试验,杂志文章


    authors: Cochran RJ,Tucker SB,Flannigan SA

    更新日期:1985-04-01 00:00:00

  • Hereditary benign telangiectasia: first case in Iran.

    abstract::A 14-year-old boy was referred to the Dermatology Clinic of the Medical University of Mashhad, Iran, with numerous cutaneous telangiectasias on the face, ears, lips, and back of the hands, with lesions in the temporal region being the first to appear (Figs 1-3). His mother stated that the lesions had been present for ...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Javidi Z,Maleki M,Mashayekhi V,Nahidi Y,Omidvar Borna A

    更新日期:2006-07-01 00:00:00

  • Organizing a dermatology service mission.

    abstract:BACKGROUND:There are few published guidelines that describe the forethought and logistical considerations needed to create a dermatology-specific medical mission. OBJECTIVE:To report the experience of planning and executing a successful medical mission to an underserved community in Puerto Rico. METHODS:We identified...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Ramirez-Fort MK,Lastra-Vicente R,Levitt JO,Sanchez JL,Reizner GT

    更新日期:2013-03-01 00:00:00

  • Pellagra probably due to pyrazinamide: development during combined chemotherapy of tuberculosis.

    abstract::Pellagra secondary to antituberculous chemotherapy can result from isoniazide therapy, as well as from pyrazinamide treatment. Because of the similarity in structural formulas between pyrazinamide, isoniazide, and nicotinamide, the substrate competition is the mechanism most likely involved. The patient was cured by a...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Jørgensen J

    更新日期:1983-01-01 00:00:00

  • Quality of life and related factors in a cohort of plaque-type psoriasis patients in La Coruña, Spain.

    abstract:BACKGROUND:Psoriasis can significantly affect the physical, psychological, and social aspects of a patient's life. Many studies have evaluated the effects of psoriasis on quality of life (QoL), but results in many cases are contradictory. OBJECTIVES:This study was conducted to assess the relationships between the char...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Fernández-Torres RM,Pita-Fernández S,Fonseca E

    更新日期:2014-11-01 00:00:00

  • Scar sarcoidosis after hyaluronic acid injection.

    abstract::A 54-year-old woman presented with a 5-month history of tender nodules in both nasolabial folds that had developed 4 months after the injection of hyaluronic acid (HA) (Restylane) for wrinkles. The patient was treated with 1.5 mg/day betamethasone for 6 days and her lesions disappeared within 1 week. About 8 days afte...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Dal Sacco D,Cozzani E,Parodi A,Rebora A

    更新日期:2005-05-01 00:00:00

  • Is narrowband ultraviolet B monotherapy effective in the treatment of pityriasis lichenoides?

    abstract:BACKGROUND:Pityriasis lichenoides (PL) is a self-limiting papulosquamous disease that may persist for years and is associated with a high relapse rate. To date, few comparative studies have investigated the efficacy of narrowband ultraviolet B (NB-UVB) phototherapy and other therapies in the treatment of PL. OBJECTIVE...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Park JM,Jwa SW,Song M,Kim HS,Chin HW,Ko HC,Kim MB,Kim BS

    更新日期:2013-08-01 00:00:00

  • Immediate nonallergic psychotic reaction to intramuscular procaine penicillin.

    abstract:BACKGROUND:Though little known by medical personnel, an immediate nonallergic psychotic reaction to intramuscular procaine penicillin has been reported occasionally from many countries since 1951. MATERIALS AND METHODS:A case report describes a patient whose violent behavior, provoked by this reaction, resulted in leg...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Einterz EM,Einterz RM

    更新日期:1995-09-01 00:00:00

  • Levocetirizine is an effective treatment in patients suffering from chronic idiopathic urticaria: a randomized, double-blind, placebo-controlled, parallel, multicenter study.

    abstract:BACKGROUND:Chronic idiopathic urticaria (CIU) is defined by the almost daily presence of urticaria for at least 6 weeks without an identifiable cause. Symptoms include short-lived wheals, itching, and erythema. CIU impedes significantly a patient's quality of life (QoL). Levocetirizine is an antihistamine from the late...

    journal_title:International journal of dermatology

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Kapp A,Pichler WJ

    更新日期:2006-04-01 00:00:00

  • In vivo pressure sore-healing efficacy of β-cyclodextrin/polyethyleneimine/silk fibroin xerogel.

    abstract:BACKGROUND:β-Cyclodextrin (βCD) xerogel containing silk fibroin (SF) was prepared, and the efficacy of the xerogel for a pressure sore developed on the back of an ICR mouse was investigated. MATERIALS AND METHODS:Tosyl βCD was conjugated to polyethyleneimine (PEI), and the βCD residues were cross-linked in an aqueous ...

    journal_title:International journal of dermatology

    pub_type: 杂志文章


    authors: Seo SR,Lee MS,So BP,Kim JC

    更新日期:2012-08-01 00:00:00

  • Elevated plasma homocysteine levels in patients on isotretinoin therapy for cystic acne.

    abstract:BACKGROUND:The use of Isotretinoin (Iso) for cystic acne (CA) therapy includes marked side-effects such as dyslipidemia, increased liver enzymes, and reduction of biotinidase activity. Moreover, Homocysteine (Hcy), an amino acid, is metabolized in the liver requiring folate, vitamin B6, vitamin B12, and the activity of...

    journal_title:International journal of dermatology

    pub_type: 临床试验,杂志文章


    authors: Schulpis KH,Karikas GA,Georgala S,Michas T,Tsakiris S

    更新日期:2001-01-01 00:00:00

  • Tamoxifen vs. non-tamoxifen treatment for advanced melanoma: a meta-analysis.

    abstract::Although tamoxifen (TAM) is routinely used in advanced melanoma, it is still uncertain whether evidence exists to support this practice. This review assesses the benefits and harms of systemic therapy with TAM vs. without TAM on response and mortality in patients with advanced melanoma. MEDLINE, The Cochrane Database ...

    journal_title:International journal of dermatology

    pub_type: 杂志文章,meta分析,评审


    authors: Beguerie JR,Xingzhong J,Valdez RP

    更新日期:2010-10-01 00:00:00

  • Treatment of histoid leprosy: a lack of consensus.

    abstract::Histoid leprosy is a rare variant of leprosy with a unique clinical presentation and bacilli rich histology. These patients are large reservoirs for disease and vectors for spread, making prompt diagnosis and treatment crucial. To date, no consensus on treatment and duration exists. This paper aims to investigate the ...

    journal_title:International journal of dermatology

    pub_type: 杂志文章,评审


    authors: Bartos G,Sheuring R,Combs A,Rivlin D

    更新日期:2020-10-01 00:00:00