Deciphering the luteal transcriptome: potential mechanisms mediating stage-specific luteolytic response of the corpus luteum to prostaglandin F₂α.


:The objective of this study was to identify prostaglandin F(2α) (PG)-induced changes in the transcriptome of bovine corpora lutea (CL) that are specific to mature, PG-responsive (day 11) CL vs. developing (day 4) CL, which do not undergo luteolysis in response to PG administration. CL were collected at 0, 4, and 24 h after PG injection on days 4 and 11 of the estrous cycle (n = 5 per day and time point), and microarray analysis was performed with GeneChip Bovine Genome Arrays. Data normalization was performed with affy package and significance testing with maanova from Bioconductor. Significance (relative to 0 h time point) was declared at fold change >2.0 or <0.5 and false discovery rate of <5%. At 4 and 24 h after PG, 221 (day 4) and 661 (day 11) and 248 (day 4) and 1,421 (day 11) regulated genes, respectively, were identified. The accentuated gene expression response in day 11 CL was accompanied by specific enrichment of PG-regulated genes in distinctive gene ontology categories (immune related and other), particularly at 24 h after injection. Specificity in putative transcription factor binding sites was observed among PG-regulated genes on day 11 vs. day 4, including a potential association of ETS transcription factors with acute PG-induced gene expression specific to day 11 CL. Temporal and PG-induced regulation of abundance of mRNA for ETS transcription factor family members linked to the stage-specific response to PG was not observed. Increased abundance of protein and/or mRNA for six PG-regulated putative ETS-responsive genes was noted in day 11 but not day 4 CL. Results reveal insight into stage-specific gene expression in bovine CL in response to PG and potential transcriptional mediators of luteolysis.


Physiol Genomics


Physiological genomics


Mondal M,Schilling B,Folger J,Steibel JP,Buchnick H,Zalman Y,Ireland JJ,Meidan R,Smith GW




Has Abstract


2011-04-27 00:00:00














  • Transcriptome of the NTS in exercise-trained spontaneously hypertensive rats: implications for NTS function and plasticity in regulating blood pressure.

    abstract::The nucleus tractus solitarii (NTS) controls the cardiovascular system during exercise, and alteration of its function may underlie exercise-induced cardiovascular adaptation. To understand the molecular basis of the NTS's plasticity in regulating blood pressure (BP) and its potential contribution to the antihypertens...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Waki H,Gouraud SS,Bhuiyan ME,Takagishi M,Yamazaki T,Kohsaka A,Maeda M

    更新日期:2013-01-07 00:00:00

  • DNA microarray analysis of vitamin D-induced gene expression in a human colon carcinoma cell line.

    abstract::The full extent to which 1,25-dihydroxyvitamin D(3) affects gene expression in human intestinal epithelial cells is unknown. We used oligonucleotide arrays to catalog vitamin D-induced changes in gene expression in Caco-2 cells, a human colon carcinoma cell line. Five paired sets of Caco-2 cell cultures were subjected...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Wood RJ,Tchack L,Angelo G,Pratt RE,Sonna LA

    更新日期:2004-04-13 00:00:00

  • Identification of positional candidate genes for body weight and adiposity in subcongenic mice.

    abstract::We previously constructed a congenic mouse, B6.S-D2Mit194-D2Mit311 (B6.S-2) with 27 Mb of SPRET/Ei donor DNA on distal chromosome 2 in a C57BL/6J background that captured an obesity quantitative trait locus (QTL). Mice homozygous for SPRET/Ei alleles at the donor region had decreased body weight and obesity-related ph...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Chiu S,Kim K,Haus KA,Espinal GM,Millon LV,Warden CH

    更新日期:2007-09-19 00:00:00

  • Profiling neuroendocrine gene expression changes following fadrozole-induced estrogen decline in the female goldfish.

    abstract::Teleost fish represent unique models to study the role of neuroestrogens because of the extremely high activity of brain aromatase (AroB; the product of cyp19a1b). Aromatase respectively converts androstenedione and testosterone to estrone and 17beta-estradiol (E2). Specific inhibition of aromatase activity by fadrozo...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Zhang D,Popesku JT,Martyniuk CJ,Xiong H,Duarte-Guterman P,Yao L,Xia X,Trudeau VL

    更新日期:2009-08-07 00:00:00

  • Na/K-ATPase signaling regulates collagen synthesis through microRNA-29b-3p in cardiac fibroblasts.

    abstract::Chronic kidney disease (CKD) is accompanied by cardiac fibrosis, hypertrophy, and dysfunction, which are commonly referred to as uremic cardiomyopathy. Our previous studies found that Na/K-ATPase ligands or 5/6th partial nephrectomy (PNx) induces cardiac fibrosis in rats and mice. The current study used in vitro and i...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Drummond CA,Hill MC,Shi H,Fan X,Xie JX,Haller ST,Kennedy DJ,Liu J,Garrett MR,Xie Z,Cooper CJ,Shapiro JI,Tian J

    更新日期:2016-03-01 00:00:00

  • Identification of genes whose expression is altered by obesity throughout the arterial tree.

    abstract::We used next-generation RNA sequencing (RNA-Seq) technology on the whole transcriptome to identify genes whose expression is consistently affected by obesity across multiple arteries. Specifically, we examined transcriptional profiles of the iliac artery as well as the feed artery, first, second, and third branch orde...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Padilla J,Jenkins NT,Thorne PK,Martin JS,Rector RS,Davis JW,Laughlin MH

    更新日期:2014-11-15 00:00:00

  • Integration of expression profiles and genetic mapping data to identify candidate genes in intracranial aneurysm.

    abstract::Intracranial aneurysm (IA) is a complex genetic disease for which, to date, 10 loci have been identified by linkage. Identification of the risk-conferring genes in the loci has proven difficult, since the regions often contain several hundreds of genes. An approach to prioritize positional candidate genes for further ...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Weinsheimer S,Lenk GM,van der Voet M,Land S,Ronkainen A,Alafuzoff I,Kuivaniemi H,Tromp G

    更新日期:2007-12-19 00:00:00

  • A floxed allele of the androgen receptor gene causes hyperandrogenization in male mice.

    abstract::We previously generated a conditional floxed mouse line to study androgen action, in which exon 3 of the androgen receptor (AR) gene is flanked by loxP sites, with the neomycin resistance gene present in intron 3. Deletion of exon 3 in global AR knockout mice causes androgen insensitivity syndrome, characterized by ge...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: MacLean HE,Chiu WS,Ma C,McManus JF,Davey RA,Cameron R,Notini AJ,Zajac JD

    更新日期:2008-03-14 00:00:00

  • Quest for arthritis-causative genetic factors in the rat.

    abstract::Experimental rat models of arthritis are extensively studied with a view to understand the genetic underpinnings of rheumatoid arthritis (RA). Genome scans using these models have led to the detection of arthritis regulatory quantitative trait loci (QTLs) on all but three chromosomes of the rat. Whereas some of the QT...

    journal_title:Physiological genomics

    pub_type: 杂志文章,评审


    authors: Joe B

    更新日期:2006-10-03 00:00:00

  • Short-term administration of rhGH increases markers of cellular proliferation but not milk protein gene expression in normal lactating women.

    abstract::Growth hormone is one of few pharmacologic agents known to augment milk production in humans. We hypothesized that recombinant human GH (rhGH) increases the expression of cell proliferation and milk protein synthesis genes. Sequential milk and blood samples collected over four days were obtained from five normal lacta...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Maningat PD,Sen P,Rijnkels M,Hadsell DL,Bray MS,Haymond MW

    更新日期:2011-04-27 00:00:00

  • Changes in behavior and gene expression induced by caloric restriction in C57BL/6 mice.

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    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Yamamoto Y,Tanahashi T,Kawai T,Chikahisa S,Katsuura S,Nishida K,Teshima-Kondo S,Sei H,Rokutan K

    更新日期:2009-11-06 00:00:00

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    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Brauch KM,Dhruv ND,Hanse EA,Andrews MT

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  • Novel technique of aortic banding followed by gene transfer during hypertrophy and heart failure.

    abstract::Aortic banding in the rat has become a popular method to induce left ventricular (LV) hypertrophy and heart failure. However, because of often extensive intrathoracic adhesions and inflammatory cell infiltrates resulting from the traditional surgical approach, an uncomplicated second thoracic incision for genetic mani...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Del Monte F,Butler K,Boecker W,Gwathmey JK,Hajjar RJ

    更新日期:2002-01-01 00:00:00

  • Changes in global gene expression in rat myometrium in transition from late pregnancy to parturition.

    abstract::The process of parturition involves the complex interplay of factors that change the excitability and contractile activity of the uterus. We have compared the relative gene expression profile of myometrium from rats before parturition (21 days pregnant) and during delivery, using high-density DNA microarray. Of 8,740 ...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Helguera G,Eghbali M,Sforza D,Minosyan TY,Toro L,Stefani E

    更新日期:2009-01-08 00:00:00

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    journal_title:Physiological genomics

    pub_type: 杂志文章,评审


    authors: Cho Y,Ross RS

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  • Knockin mice with Leu9'Ser alpha4-nicotinic receptors: substantia nigra dopaminergic neurons are hypersensitive to agonist and lost postnatally.

    abstract::This study analyzes the electrophysiological cause and behavioral consequence of dopaminergic cell loss in a knockin mouse strain bearing hypersensitive nicotinic alpha4-receptor subunits ("L9'S mice"). Adult brains of L9'S mice show moderate loss of substantia nigra dopaminergic neurons and of striatal dopaminergic i...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Orb S,Wieacker J,Labarca C,Fonck C,Lester HA,Schwarz J

    更新日期:2004-08-11 00:00:00

  • Gene expression in hypothalamus and brown adipose tissue of mice divergently selected for heat loss.

    abstract::Gene expression was evaluated in mice divergently selected for 16 generations for heat loss, measured by direct calorimetry. The high (MH) heat loss line has approximately 50% greater heat loss, approximately 35% less body fat, approximately 20% greater feed intake, and twofold greater activity levels than the low (ML...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Allan MF,Nielsen MK,Pomp D

    更新日期:2000-09-08 00:00:00

  • From raw materials to validated system: the construction of a genomic library and microarray to interpret systemic perturbations in Northern bobwhite.

    abstract::The limited availability of genomic tools and data for nonmodel species impedes computational and systems biology approaches in nonmodel organisms. Here we describe the development, functional annotation, and utilization of genomic tools for the avian wildlife species Northern bobwhite (Colinus virginianus) to determi...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Rawat A,Gust KA,Deng Y,Garcia-Reyero N,Quinn MJ Jr,Johnson MS,Indest KJ,Elasri MO,Perkins EJ

    更新日期:2010-07-07 00:00:00

  • Ammonia excretion in rainbow trout (Oncorhynchus mykiss): evidence for Rh glycoprotein and H+-ATPase involvement.

    abstract::Branchial ammonia transport in freshwater teleosts is not well understood. Most studies conclude that NH(3) diffuses out of the gill and becomes protonated to NH(4)(+) in an acidified gill boundary layer. Rhesus (Rh) proteins are new members of the ammonia transporter superfamily and rainbow trout possess genes encodi...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Nawata CM,Hung CC,Tsui TK,Wilson JM,Wright PA,Wood CM

    更新日期:2007-11-14 00:00:00

  • Genomic dissection of the cytokine-controlled STAT5 signaling network in liver.

    abstract::Growth hormone (GH) controls the physiology and pathophysiology of the liver, and its signals are conducted by two members of the family of signal transducers and activators of transcription, STAT5A and STAT5B. Mice in which the Stat5a/b locus has been inactivated specifically in hepatocytes display GH resistance, the...

    journal_title:Physiological genomics

    pub_type: 杂志文章,评审


    authors: Hosui A,Hennighausen L

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  • Genomic profiling of developing cardiomyocytes from recombinant murine embryonic stem cells reveals regulation of transcription factor clusters.

    abstract::Cardiomyocytes derived from pluripotent embryonic stem cells (ESC) have the advantage of providing a source for standardized cell cultures. However, little is known on the regulation of the genome during differentiation of ESC to cardiomyocytes. Here, we characterize the transcriptome of the mouse ESC line CM7/1 durin...

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    pub_type: 杂志文章


    authors: Seewald MJ,Ellinghaus P,Kassner A,Stork I,Barg M,Niebrügge S,Golz S,Summer H,Zweigerdt R,Schräder EM,Feicht S,Jaquet K,Reis S,Körfer R,Milting H

    更新日期:2009-06-10 00:00:00

  • Noninjurious mechanical ventilation activates a proinflammatory transcriptional program in the lung.

    abstract::Mechanical ventilation is a life-saving intervention in patients with respiratory failure. However, human and animal studies have demonstrated that mechanical ventilation using large tidal volumes (>or=12 ml/kg) induces a potent inflammatory response and can cause acute lung injury. We hypothesized that mechanical ven...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Gharib SA,Liles WC,Klaff LS,Altemeier WA

    更新日期:2009-05-13 00:00:00

  • High-fat diet negatively impacts both metabolic and behavioral health in outbred heterogeneous stock rats.

    abstract::Obesity is influenced by genetics and diet and has wide ranging comorbidities, including anxiety and depressive disorders. Outbred heterogeneous stock (HS) rats are used for fine-genetic mapping of complex traits and may be useful for understanding gene by diet interactions. In this study, HS rats were fed diets conta...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Deal AW,Seshie O,Lenzo A,Cooper N,Ozimek N,Solberg Woods LC

    更新日期:2020-09-01 00:00:00

  • Thermoprotection of synaptic transmission in a Drosophila heat shock factor mutant is accompanied by increased expression of Hsp83 and DnaJ-1.

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    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Neal SJ,Karunanithi S,Best A,So AK,Tanguay RM,Atwood HL,Westwood JT

    更新日期:2006-05-16 00:00:00

  • Functional properties of skeletal muscle from transgenic animals with upregulated heat shock protein 70.

    abstract::The influence of inducible heat stress proteins on protecting contracting skeletal muscle against fatigue-induced injury was investigated. A line of transgenic mice overexpressing the inducible form of the 72-kDa heat shock protein (HSP72) in skeletal muscles was used. We examined the relationship between muscle contr...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Nosek TM,Brotto MA,Essig DA,Mestril R,Conover RC,Dillmann WH,Kolbeck RC

    更新日期:2000-11-09 00:00:00

  • In silico structure-function analysis of pathological variation in the HSD11B2 gene sequence.

    abstract::11beta-Hydroxysteroid dehydrogenase type 2 (11betaHSD2) is a short-chain dehydrogenase/reductase (SDR) responsible for inactivating cortisol and preventing its binding to the mineralocorticoid receptor (MR). Nonfunctional mutations in HSD11B2, the gene encoding 11betaHSD2, cause the hypertensive syndrome of apparent m...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Manning JR,Bailey MA,Soares DC,Dunbar DR,Mullins JJ

    更新日期:2010-08-01 00:00:00

  • Transfer of the Rf-1 region from FHH onto the ACI background increases susceptibility to renal impairment.

    abstract::The genetically hypertensive fawn-hooded (FHH/Eur) rat is characterized by the early presence of systolic and glomerular hypertension, progressive proteinuria (UPV), and albuminuria (UAV), and focal glomerulosclerosis, resulting in premature death from renal failure. Previous studies showed that at least five genetic ...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Provoost AP,Shiozawa M,Van Dokkum RP,Jacob HJ

    更新日期:2002-02-28 00:00:00

  • Blood transcriptional signature of recombinant human erythropoietin administration and implications for antidoping strategies.

    abstract::Recombinant human erythropoietin (rHuEPO) is frequently abused by athletes as a performance-enhancing drug, despite being prohibited by the World Anti-Doping Agency. Although the methods to detect blood doping, including rHuEPO injections, have improved in recent years, they remain imperfect. In a proof-of-principle s...

    journal_title:Physiological genomics

    pub_type: 临床试验,杂志文章


    authors: Durussel J,Haile DW,Mooses K,Daskalaki E,Beattie W,Mooses M,Mekonen W,Ongaro N,Anjila E,Patel RK,Padmanabhan N,McBride MW,McClure JD,Pitsiladis YP

    更新日期:2016-03-01 00:00:00

  • Distinct profiles of expressed sequence tags during intestinal regeneration in the sea cucumber Holothuria glaberrima.

    abstract::Repair and regeneration are key processes for tissue maintenance, and their disruption may lead to disease states. Little is known about the molecular mechanisms that underline the repair and regeneration of the digestive tract. The sea cucumber Holothuria glaberrima represents an excellent model to dissect and charac...

    journal_title:Physiological genomics

    pub_type: 杂志文章


    authors: Rojas-Cartagena C,Ortíz-Pineda P,Ramírez-Gómez F,Suárez-Castillo EC,Matos-Cruz V,Rodríguez C,Ortíz-Zuazaga H,García-Arrarás JE

    更新日期:2007-10-22 00:00:00

  • Novel slc22 transporter homologs in fly, worm, and human clarify the phylogeny of organic anion and cation transporters.

    abstract::Slc22 family organic anion and cation transporters (OATs, OCTs, and OCTNs) are transmembrane proteins expressed predominantly in kidney and liver. These proteins mediate the uptake or excretion of numerous physiologically (and pharmacologically) important compounds, and accordingly have been the focus of intensive stu...

    journal_title:Physiological genomics

    pub_type: 杂志文章,评审


    authors: Eraly SA,Monte JC,Nigam SK

    更新日期:2004-06-17 00:00:00