Saucerneol G, a new lignan, from Saururus chinensis inhibits matrix metalloproteinase-9 induction via a nuclear factor κB and mitogen activated protein kinases in lipopolysaccharide-stimulated RAW264.7 cells.


:This study was conducted to demonstrate the inhibitory effect of saucerneol G (SG), a new lignan, isolated from the aerial part of Saururus chinensis (Saururaceae) on lipopolysaccharide (LPS)-stimulated matrix metalloproteinase-9 (MMP)-9 inductions in RAW 264.7 cells. Aimed at evaluating the mechanism of action by which SG inhibits the LPS-mediated induction of MMP-9, the effects of SG on nuclear factor-κB (NF-κB) DNA binding activity, NF-κB-dependent reporter gene activity, inhibitory factor-κB (IκB) phosphorylation, degradation and p65 nuclear translocation were assessed. SG profoundly suppressed the DNA binding activity and the reporter gene activity as well as translocation of NF-κB p65 subunit. Furthermore, SG also dose dependently inhibited LPS-stimulated activation of mitogen-activated protein kinases (MAPKs). These findings suggest that SG may inhibit LPS-stimulated MMP-9 induction by blocking NF-κB and MAPKs activation.


Biol Pharm Bull


Lu Y,Hong TG,Jin M,Yang JH,Suh SJ,Piao DG,Ko HK,Seo CS,Chang YC,Kim CH,Son JK,Chang HW




Has Abstract


2010-01-01 00:00:00














  • Characterization of nuclear factors that bind to the human thymidylate synthase gene in HL-60 cells differentiated by all-trans retinoic acid treatment.

    abstract::HL-60 cells differentiate into granulocyte-like cells by all-trans retinoic acid (ATRA) treatment, and the cellular proliferation is markedly reduced during the differentiation. To elucidate the molecular mechanisms of the growth arrest during the cellular differentiation, we examined the regulated expression of the t...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Horie N,Komada Y,Ueta Y,Suzuki T,Nozawa R,Takeishi K

    更新日期:2001-12-01 00:00:00

  • Reducing the impact of binding of UCN-01 to human alpha1-acid glycoprotein by encapsulation in liposomes.

    abstract::A liposomal formulation of UCN-01 was studied to prevent binding of drug to human alpha1-acid glycoprotein (hAGP). The release of drug from liposomes added to various media was investigated by monitoring the concentration of UCN-01 in different fractions. Protein bound UCN-01 was separated from liposomal UCN-01 and fr...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Yamauchi M,Kusano H,Nakakura M,Kato Y

    更新日期:2005-07-01 00:00:00

  • Prolonged intestinal absorption of cephradine with chitosan-coated ethylcellulose microparticles in rats.

    abstract::Cephradine-containing ethylcellulose microparticles (MPC) were prepared by the solvent evaporation method. Chitosan-coated MPC (Chi-MPC) were prepared by doping MPC with viscous chitosan solution and subsequently drying. When fluorescein isothiocyanate (FITC)-labeled chitosan-coated ethylcellulose microparticles witho...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Takishima J,Onishi H,Machida Y

    更新日期:2002-11-01 00:00:00

  • Genetic engineering of transgenic tobacco for enhanced uptake and bioaccumulation of mercury.

    abstract::To further enhance the efficiency and potential of plants for phytoremediation of mercury pollution, a genetically engineered tobacco to simultaneously express mercury transporter, mercury transporter (MerT) and mercury chelator, polyphosphate (polyP) was constructed by integrating bacterial merT gene in polyphosphate...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Nagata T,Nakamura A,Akizawa T,Pan-Hou H

    更新日期:2009-09-01 00:00:00

  • Effect of N-B transition on the microenvironment surrounding 34Cys in human serum albumin.

    abstract::The effect of pH on the microenvironment surrounding 34Cys in human serum albumin (HSA) has been studied using acrylodan, a Cys-specific fluorescence probe. The reactivity of 34Cys with 5,5'-dithiobis(2-nitro benzoic acid) (DTNB) followed a pseudo-first-order reaction, and the increase in reactivity was dependent on p...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Narazaki R,Maruyama T,Otagiri M

    更新日期:1997-04-01 00:00:00

  • Checkpoint kinase 1 is cleaved in a caspase-dependent pathway during genotoxic stress-induced apoptosis.

    abstract::Checkpoint kinase 1 (Chk1) plays important roles in genotoxic stress-induced cell cycle checkpoint and in normal cell cycle progression. Here, we show that Chk1 is cleaved in the treatment of apoptotic dose of etoposide (ETP) or cisplatin (CIS) but not of viable dose in HeLa S3 cells. The cleavage of Chk1 was complete...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Okita N,Kudo Y,Tanuma S

    更新日期:2007-02-01 00:00:00

  • Hepatic injury-specific conversion of mouse plasma hyaluronan binding protein to the active hetero-dimer form.

    abstract::Plasma hyaluronan binding protein (PHBP) is produced only in liver and kidney in mouse. The induction of PHBP mRNA and the conversion of pro PHBP to the active hetero-dimer form were studied after CCl4, D-galactosamine, HgCl2 or turpentine administration and after partial hepatectomy. The results indicated that the ad...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Choi-Miura NH,Otsuyama K,Sano Y,Saito K,Takahashi K,Tomita M

    更新日期:2001-08-01 00:00:00

  • Dehydrocholic Acid Ameliorates Sodium Taurocholate-Induced Acute Biliary Pancreatitis in Mice.

    abstract::Acute biliary pancreatitis (ABP) with a high mortality rate is an incurable digestive system disease induced by abnormal bile acid regurgitation due to the biliary obstruction. Dehydrocholic acid (DA) alleviates the severity of cholestatic hepatitis related to biliary inflammation, suggesting DA is potential to develo...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Zhang X,Xin G,Li S,Wei Z,Ming Y,Yuan J,Wen E,Xing Z,Yu K,Li Y,Zhang J,Zhang B,Niu H,Huang W

    更新日期:2020-01-01 00:00:00

  • Acute cytotoxic effects of mercuric compounds in cultured astrocytes prepared from cerebral hemisphere and cerebellum of newborn rats.

    abstract::We investigated acute cytotoxic effects and Hg accumulation after exposure to methylmercury (MeHg) or Hg(2+) in the presence or absence of serum in cultured astrocytes prepared from the cerebral hemisphere or cerebellum of newborn rats. Dose-related changes in viable cell numbers after exposure to mercuric compounds w...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Adachi T,Kunimoto M

    更新日期:2005-12-01 00:00:00

  • Species identification of licorice using nrDNA and cpDNA genetic markers.

    abstract::For the accurate identification of medicinal licorice species, nucleotide sequences of four types of DNA regions were researched for 205 specimens, including three species used as licorice: Glycyrrhiza uralensis, Glycyrrhiza glabra, and Glycyrrhiza inflata. The four DNA regions were the internal transcribed spacer (IT...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kondo K,Shiba M,Yamaji H,Morota T,Zhengmin C,Huixia P,Shoyama Y

    更新日期:2007-08-01 00:00:00

  • Probing AKR1C30 and AKR1C31 with site-directed mutagenesis: identifying the roles of residues 54 and 56 in the binding of substrates and inhibitors.

    abstract::Five rabbit aldo-keto reductases (AKRs) that participate in the reduction of drug ketones and endogenous ketosteroids have recently been cloned and characterized. Among them, AKR1C30 and AKR1C31 show the highest amino acid sequence identity of 91%, but markedly differ in their substrate specificity and inhibitor sensi...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Endo S,Arai Y,Matsunaga T,Ikari A,El-Kabbani O,Hara A,Kitade Y

    更新日期:2014-01-01 00:00:00

  • Cloning of polyketide synthase genes from amphidinolide-producing dinoflagellate Amphidinium sp.

    abstract::Cloning of polyketide synthase (PKS) gene for amphidinolide biosynthesis was attempted from a dinoflagellate Amphidinium sp. (strain Y-42). Fourteen beta-ketoacyl synthase gene fragments were obtained by Polymerase Chain Reaction (PCR) amplification from degenerated primer sets designed on the basis of the conserved a...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kubota T,Iinuma Y,Kobayashi J

    更新日期:2006-07-01 00:00:00

  • The in vitro antiosteoporotic activity of some glycosides in Dioscorea spongiosa.

    abstract::In the investigation of chemical constituents in antiosteoporotic active fraction, 90% ethanol fraction of water extract of the rhizomes of Dioscorea spongiosa, twenty-two glycosides were isolated and their structures were elucidated. In order to know the role of each compound played in the fraction and to find the ex...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Yin J,Han N,Liu Z,Song S,Kadota S

    更新日期:2010-01-01 00:00:00

  • Effect of the hot water extract of Artocarpus camansi leaves on 2,4,6-trinitrochlorobenzene (TNCB)-induced contact hypersensitivity in mice.

    abstract::Medicinal plants with reported anti-inflammatory activity could have the potential use as anti-allergens and inhibitors of allergic contact dermatitis reactions produced by allergens and chemicals. Some species from the genus Artocarpus were reported to have anti-inflammatory activity. In the Philippines one local sou...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Salonga RB,Hisaka S,Nose M

    更新日期:2014-01-01 00:00:00

  • Antioxidant activities of chitobiose and chitotriose.

    abstract::Chitooligosaccharides, the oligomers made up of beta-1,4-linked D-glucosamine, are obtained by partial hydrolysis of chitosan, a deacetylation product of chitin. The antioxidant activity of various chitooligosaccharides was tested in vitro with aminoguanidine, pyridoxamine, and Trolox as reference compounds. Hydroxyla...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Chen AS,Taguchi T,Sakai K,Kikuchi K,Wang MW,Miwa I

    更新日期:2003-09-01 00:00:00

  • Effect of 12-O-tetradecanoylphorbol-13-acetate on expression of cell surface antigens in two subclones of human leukemia K562 cells.

    abstract::Two subclones (K562-L and -H) were previously isolated from K562 human leukemic cells according to hemoglobin production: K562-L was expressed in less than 5% and K562-H in more than 90% of dianisidine positive cells. 12-O-Tetradecanoylphorbol-13- acetate (TPA) suppressed the expression of the erythrocytic (glycophori...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Sasaki D,Kosunago S,Mikami T,Matsumoto T,Suzuki M

    更新日期:1993-10-01 00:00:00

  • Hepatoprotective effects of phloridzin on hepatic fibrosis induced by carbon tetrachloride against oxidative stress-triggered damage and fibrosis in rats.

    abstract::The present study was to study the hepatoprotective effects of phloridzin (PHL) on hepatic fibrosis induced by carbon tetrachloride (CCl₄) in rats, on the basis of this investigation, the possible mechanism of PHL was elucidated. Male Sprague Dawley (SD) rats were randomly divided into six groups: control, model, PHL-...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Deng G,Wang J,Zhang Q,He H,Wu F,Feng T,Zhou J,Zou K,Hattori M

    更新日期:2012-01-01 00:00:00

  • Nucleolin Acts as a Scavenger Receptor for Acetylated Low-Density Lipoprotein on Macrophages.

    abstract::Although macrophage phagocytoses modified low-density lipoprotein (LDL), excessive accumulation of modified LDL induces macrophage foam cell formation, which is a feature of atherosclerotic plaque. Thus, the identification of scavenger receptor for modified LDL will provide better understanding of an atherosclerotic e...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Miki Y,Tachibana Y,Ohminato Y,Fujiwara Y

    更新日期:2015-01-01 00:00:00

  • Localization and activity of adrenal carbonyl reductase during the estrous cycle.

    abstract::Changes in carbonyl reductase (CR) activity towards seven substrates in adrenal glands of female rats were investigated during estrous cycle and the circadian patterns of adrenal CR activity towards seven substrates in male rats were examined. The localization of the enzyme protein in the adrenal gland was carried out...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Inazu N,Nagashima Y,Satoh T

    更新日期:1995-05-01 00:00:00

  • Preventive effect of 7,8-dihydroxyflavone against oxidative stress induced genotoxicity.

    abstract::We elucidated the protective effect of 7,8-dihydroxyflavone against hydrogen peroxide (H(2)O(2))-induced DNA damage. We found that 7,8-dihydroxyflavone scavenges 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and intracellular reactive oxygen species (ROS). 7,8-Dihydroxyflavone with antioxidant effect prevented the H(2)...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Zhang R,Kang KA,Piao MJ,Ko DO,Wang ZH,Chang WY,You HJ,Lee IK,Kim BJ,Kang SS,Hyun JW

    更新日期:2009-02-01 00:00:00

  • Prenylflavones from Psoralea corylifolia inhibit nitric oxide synthase expression through the inhibition of I-kappaB-alpha degradation in activated microglial cells.

    abstract::The overproduction of nitric oxide (NO) by inducible nitric oxide synthase (iNOS) switches the function of NO from a physiological neuromodulator to a neurotoxic effector in central nervous system (CNS) after brain injury. From the methanol extracts of Psoralea corylifolia, we purified two inhibitors of NO production ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Lee MH,Kim JY,Ryu JH

    更新日期:2005-12-01 00:00:00

  • O/W lipid emulsions for parenteral drug delivery. I. Pharmacokinetics of the oil particles and incorporated sudan II.

    abstract::The potential usefulness of oil in water (O/W) lipid emulsions as parenteral drug delivery system for lipophilic drugs was examined in tumor-bearing rats. A model lipophilic drug, sudan II (PCoct = 226000), was formulated in five lipid emulsions consisting of soybean oil and various surfactants. Compared with HCO-60 m...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Sakaeda T,Takahashi K,Nishihara Y,Hirano K

    更新日期:1994-11-01 00:00:00

  • Pharmacological activity of deep-sea water: examination of hyperlipemia prevention and medical treatment effect.

    abstract::When normal rabbits were administered various samples of deep-sea water, their biochemical values changed within normal limits, and no differences from distilled water administration (control) group levels were observed. Furthermore, no histopathological changes were observed in internal organs on the 28th day after a...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Yoshioka S,Hamada A,Cui T,Yokota J,Yamamoto S,Kusunose M,Miyamura M,Kyotani S,Kaneda R,Tsutsui Y,Odani K,Odani I,Nishioka Y

    更新日期:2003-11-01 00:00:00

  • Differential induction of chromosomal aberrations by topoisomerase inhibitors in cultured Chinese hamster cells.

    abstract::The inducibility of chromosomal aberration was studied using five mammalian topoisomerase inhibitors in Chinese hamster lung fibroblastic cells (CHL) in two protocols involving 6 and 24 h treatment, with respect to the mechanism of chromosomal aberration. In the 6 h treatment plus 18 h recovery culture (6 h treatment)...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Suzuki H,Nakane S

    更新日期:1994-02-01 00:00:00

  • Preparation and in vivo evaluation of piroxicam-loaded gelatin microcapsule by spray drying technique.

    abstract::To develop a piroxicam-loaded gelatin microcapsule with enhanced bioavailability, a gelatin microcapsule encapsulated ethanol and piroxicam has been formulated by using gelatin as a water-soluble polymer shell. The aqueous solubility and bioavailability of piroxicam in piroxicam-loaded microcapsule in rats were then e...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Piao MG,Yang CW,Li DX,Kim JO,Jang KY,Yoo BK,Kim JA,Woo JS,Lyoo WS,Han SS,Lee YB,Kim DD,Yong CS,Choi HG

    更新日期:2008-06-01 00:00:00

  • Improvement of the Solubility and Intestinal Absorption of Curcumin by N-Acyl Taurates and Elucidation of the Absorption-Enhancing Mechanisms.

    abstract::In this study, the effects of N-acyl taurates (NATs) on the intestinal absorption of curcumin (CUR), a water-insoluble and poorly absorbed compound, were examined in rats. Sodium methyl lauroyl taurate (LMT) and sodium methyl cocoyl taurate (CMT) were the most effective in increasing the solubility and intestinal abso...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Li X,Kawamura A,Sato Y,Morishita M,Kusamori K,Katsumi H,Sakane T,Yamamoto A

    更新日期:2017-01-01 00:00:00

  • Antiproliferative constituents in plants 9. Aerial parts of Lippia dulcis and Lippia canescens.

    abstract::The antiproliferative constituents in the MeOH extracts of the aerial parts of Lippia dulcis Trev. and Lippia canescens Kunth (Verbenaceae) were investigated. Activity-guided chemical investigation of the MeOH extracts resulted in the isolation of the three bisabolane-type sesquiterpenes [(+)-hernandulcin (1), (-)-epi...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Abe F,Nagao T,Okabe H

    更新日期:2002-07-01 00:00:00

  • The 5-HT1A receptor full agonist, 8-OH-DPAT inhibits ACTH-induced 5-HT2A receptor hyperfunction in rats: involvement of 5-HT1A receptors in the DOI-induced wet-dog shakes in ACTH-treated rats.

    abstract::We examined the influence of 8-hydroxy-2-di-n-propylamino tetralin (8-OH-DPAT), a serotonin 1A (5-HT1A) receptor full agonist, on the wet-dog shake response induced by the (+/-)-1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI), a 5-HT2A receptor agonist, in adrenocorticotropic hormone (ACTH)-treated rats. Chronic A...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kitamura Y,Kitagawa K,Fujitani Y,Shibata K,Araki H,Sendou T,Gomita Y

    更新日期:2007-01-01 00:00:00

  • Inhibitory effects of Agaricus blazei on mast cell-mediated anaphylaxis-like reactions.

    abstract::Agaricus blazei is a medicinal mushroom native to Brazil. It used to be a source of anti-tumor and immunmoactive compounds and considered a health food in many countries. However, its specific effect against mast cell-mediated anaphylactic reactions is still unknown. In the present study, we investigated the effect of...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Choi YH,Yan GH,Chai OH,Choi YH,Zhang X,Lim JM,Kim JH,Lee MS,Han EH,Kim HT,Song CH

    更新日期:2006-07-01 00:00:00

  • Effect of magnesium deficiency on intracellular ATP levels in human lens epithelial cells.

    abstract::Cataractous lenses have an altered distribution of the intracellular ionic environment, and the lens ionic imbalance with increased levels of calcium (Ca2+) and sodium (Na+), coupled with decreased levels of magnesium (Mg2+) and potassium (K+), is related to cataract development in human senile cataracts. We previousl...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Nagai N,Fukuhata T,Ito Y

    更新日期:2007-01-01 00:00:00