HsMAD2 mRNA expression may be a predictor of sensitivity to paclitaxel and survival in neuroblastoma.

Abstract:

PURPOSE:We investigated the associations between hsMAD2 mRNA expression in tumor cells and sensitivity to paclitaxel or patient prognosis in neuroblastoma. METHODS:Fifty-one formalin-fixed paraffin-embedded tumor samples were manually microdissected to collect tumor cells, and RNA was purified. Nineteen clinical samples of advanced neuroblastoma showed appropriate quality of the isolated RNA for real-time reverse transcription-polymerase chain reaction (RT-PCR) analyses. The hsMAD2 expression levels were determined by real-time RT-PCR in 4 neuroblastoma cell lines and 19 clinical samples. The sensitivity to paclitaxel was assessed by WST-8 colorimetric assays and flow cytometry. HsMAD2 expression of the clinical samples was investigated for its association with prognosis in advanced neuroblastoma patients. RESULTS:There was a significant positive correlation between hsMAD2 mRNA expression and the sensitivity to paclitaxel in four neuroblastoma cell lines. High hsMAD2 expression may be correlated with paclitaxel-induced apoptosis. Kaplan-Meier survival curves were stratified by hsMAD2 expression using the median value as a cut-off point and analyzed for prognostic significance by the log-rank test (P = 0.0467). Furthermore, multivariate survival analysis revealed that only hsMAD2 expression had a significant impact on the overall survival rate. CONCLUSIONS:Our results may warrant clinical application of paclitaxel in neuroblastoma treatment for poor prognosis patients.

journal_name

Pediatr Surg Int

authors

Otake K,Uchida K,Tanaka K,Koike Y,Inoue M,Matsushita K,Ueeda M,Hashimoto K,Kitajima T,Komada Y,Kusunoki M

doi

10.1007/s00383-010-2780-5

subject

Has Abstract

pub_date

2011-02-01 00:00:00

pages

217-23

issue

2

eissn

0179-0358

issn

1437-9813

journal_volume

27

pub_type

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