Abstract:
PURPOSE:We investigated the associations between hsMAD2 mRNA expression in tumor cells and sensitivity to paclitaxel or patient prognosis in neuroblastoma. METHODS:Fifty-one formalin-fixed paraffin-embedded tumor samples were manually microdissected to collect tumor cells, and RNA was purified. Nineteen clinical samples of advanced neuroblastoma showed appropriate quality of the isolated RNA for real-time reverse transcription-polymerase chain reaction (RT-PCR) analyses. The hsMAD2 expression levels were determined by real-time RT-PCR in 4 neuroblastoma cell lines and 19 clinical samples. The sensitivity to paclitaxel was assessed by WST-8 colorimetric assays and flow cytometry. HsMAD2 expression of the clinical samples was investigated for its association with prognosis in advanced neuroblastoma patients. RESULTS:There was a significant positive correlation between hsMAD2 mRNA expression and the sensitivity to paclitaxel in four neuroblastoma cell lines. High hsMAD2 expression may be correlated with paclitaxel-induced apoptosis. Kaplan-Meier survival curves were stratified by hsMAD2 expression using the median value as a cut-off point and analyzed for prognostic significance by the log-rank test (P = 0.0467). Furthermore, multivariate survival analysis revealed that only hsMAD2 expression had a significant impact on the overall survival rate. CONCLUSIONS:Our results may warrant clinical application of paclitaxel in neuroblastoma treatment for poor prognosis patients.
journal_name
Pediatr Surg Intjournal_title
Pediatric surgery internationalauthors
Otake K,Uchida K,Tanaka K,Koike Y,Inoue M,Matsushita K,Ueeda M,Hashimoto K,Kitajima T,Komada Y,Kusunoki Mdoi
10.1007/s00383-010-2780-5subject
Has Abstractpub_date
2011-02-01 00:00:00pages
217-23issue
2eissn
0179-0358issn
1437-9813journal_volume
27pub_type
杂志文章abstract::Repair of giant inguino-scrotal hernia (GISH) in male infants is a difficult operation, even in experienced hands. It requires an immaculate technique to avoid known complications such as tearing of the sac, injury to delicate testicular vessels and dividing of vas deferens. Moreover, a recurrence rate of 9% is noted ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-008-2145-5
更新日期:2008-06-01 00:00:00
abstract:PURPOSE:The use of general anesthesia in young children has come under increasing scrutiny due to its potential long-term neurotoxic effects. Meanwhile, regional anesthesia for surgical procedures in neonates has many advantages, including preservation of respiratory status and faster return to feeding. We describe the...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-016-4027-6
更新日期:2017-03-01 00:00:00
abstract::To evaluate the efficacy of ultrasound (US) guided percutaneous sclerotherapy in the pediatric population, 14 hydatid liver cysts (HLC) in eight male patients whose ages ranged between 6 and 16 years (mean 9.9 years) were treated. The maximum diameter was 110 mm. Albendazole was administered orally to all patients for...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000356
更新日期:2000-01-01 00:00:00
abstract::Management of stage IV rhabdomyosarcoma comprises systemic chemotherapy with local control by conservative surgery and radiotherapy. Abdominal radiotherapy may lead to radiation enteritis causing such serious morbidity as malabsorption, fistulae or stricture formation. The risk increases with the dose of radiation and...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-005-1490-x
更新日期:2005-09-01 00:00:00
abstract::The presacral cystic teratoma in an infant is reported. The cyst consisted solely of central nervous system tissue. This pathologic finding is extremely rare. A review of the literature reveals no previous report of a teratoma arising in this manner. ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-002-0831-2
更新日期:2002-12-01 00:00:00
abstract:BACKGROUND:Variation exists in the diagnostic testing for gastroesophageal reflux (GER) in infants and in the application of surgical therapy. There has been an increase in antireflux surgery (ARS) since the development of laparoscopy but the outcomes in high-risk infants is unclear. This study examines the results of ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-018-4281-x
更新日期:2018-07-01 00:00:00
abstract:PURPOSE:The Nuss procedure is a minimally invasive procedure for the correction of pectus excavatum. It involves insertion of a substernal metal bar. A feared complication of any implantation procedure is infection, which often requires removal of the implanted device. This report describes the authors' experience with...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-012-3145-z
更新日期:2012-09-01 00:00:00
abstract::Between 1974 and 1988, 86 newborns with perforated necrotizing enterocolitis (NEC) were treated by either laparotomy (usually involving a bowel resection and a temporary stoma) or a peritoneal drain under local anesthesia. The survival of babies in the laparotomy group was 57% versus 59% in the drained group. However,...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract:BACKGROUND:Retinoids are essential for fetal and lung development. Beta-carotene(BC) is the main dietary retinoid source and beta-carotene-15,15'-oxygenase-1 and 2 (Bcmo1,2) is the primary enzyme generating retinoid from BC in adult mammalian tissues. Placenta has a major role in the retinol homeostasis in fetal life: ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-014-3621-8
更新日期:2015-01-01 00:00:00
abstract::An infant born in the 34th week of gestation weighing 5,355 g with a massive sacrococcygeal (SC) tumor was delivered by elective cesarean section. An ultrasonographic examination showed solid and cystic components in the tumor. Resection was successfully undertaken with insertion of a Nélaton catheter into the rectum ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000451
更新日期:2001-03-01 00:00:00
abstract::Two patients who presented with a history of right inguinal hernia and a third with an impalpable left testis were found to have transverse testicular ectopia. Both testes were fixed in their own scrotum, through a modified Ombredanne operation in two patients and a subdartos pouch in one. ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract:UNLABELLED:Necrotizing enterocolitis (NEC) is a serious condition with a high morbidity and mortality commonly affecting premature babies. Data for the impact of the long-term disease burden in developing countries are limited although poor long-term outcome of surgically managed patients has been shown in terms of inc...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-010-2583-8
更新日期:2010-04-01 00:00:00
abstract:AIM:The aim of our study is to introduce a new objective method of perioperative evaluation of the size of diaphragmatic defect to enable comparison of results among various centres and methods used for diaphragmatic reconstruction. MATERIALS AND METHODS:Prospective observational study of neonates with congenital diap...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-012-3113-7
更新日期:2012-10-01 00:00:00
abstract::This study describes a subset of patients with posterior urethral valves (PUV) who presented late in childhood. The objective was to identify factors that lead to back-pressure effects on the upper tracts, which persist in spite of adequate valve ablation in some patients, and seek factors that may preserve the upper ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050384
更新日期:1998-09-01 00:00:00
abstract:INTRODUCTION:Bladder Bowel Dysfunction (BBD) has been described in patients with Down's Syndrome (DS). Our aim was to report the incidence, demographics, presentation, complications and management of the bladder in DS patients with BBD. METHODS:A systematic review was performed using PRISMA guidelines and search terms...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-020-04682-4
更新日期:2020-07-01 00:00:00
abstract::The majority of congenital diaphragmatic hernias (CDH) occur through the foramen of Bochdalek; herniation through the foramen of Morgagni (MH) is rare. Fifteen children (12 males and 3 females) with congenital MH (7 right, 3 left, 5 bilateral) were treated over a period of 15 years, comprising 11% of a total of 135 ch...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830100653
更新日期:2002-03-01 00:00:00
abstract:PURPOSE:The aim of this study was to analyze the outcomes of children added to the waiting list for deceased donor liver transplantation (DDLT) and the results of DDLT in a single Japanese center. METHODS:Forty-seven children were listed on the organ allocation system for DDLT. The priority points related to the medic...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-013-3381-x
更新日期:2013-11-01 00:00:00
abstract::Hirschsprung's disease (HD) in Down's syndrome (DS) patients is stated to have a worse outcome than HD alone. In our study we reviewed the immediate and long-term outcomes of these children and questioned whether DS should influence the operative management. Data were collected on all children with HD (including total...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-005-1617-0
更新日期:2006-02-01 00:00:00
abstract::Negative pressure wound therapy (NPWT) is an accepted form of treatment in managing complex wounds in adults and children. It is not as widely used in the neonatal population due to limited clinical experience. We describe the application of the RENASYS™ system (Smith and Nephew, UK) in delivering NPWT to four neonate...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-011-2868-6
更新日期:2011-08-01 00:00:00
abstract::Our presentation of four cases demonstrates the essential features of limb-body wall complex (LBWC), representing a compound anomaly pattern in body-wall defects. The diagnosis of this entity is based on two of the three following characteristics: (1) exencephaly/encephalocele and facial clefts; (2) thoraco- and/or ab...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000497
更新日期:2001-07-01 00:00:00
abstract:PURPOSE:This study aimed to evaluate the mid-term outcomes of single-stage modified anterior sagittal anorectoplasty (ASARP) for anorectal malformation with vestibular fistula. METHOD:Twenty-six patients with congenital imperforate anus and vestibular fistula underwent single-stage modified sphincter-saving ASARP betw...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-015-3749-1
更新日期:2015-09-01 00:00:00
abstract::Hypospadias surgery is one of the most difficult areas in pediatric urology and has been characterized by constant evolution. Some of the surgical techniques proposed in the past are now considered inadequate because of an unacceptable complication rate or poor functional and aesthetic results. The key for assessing a...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-004-1147-1
更新日期:2004-03-01 00:00:00
abstract::Gastrointestinal atresia is a major cause of bowel obstruction in the newborn. Experimental models and clinical observations have demonstrated the heterogeneous nature of its pathogenesis. A proportion is due to late intra-uterine vascular insults and some are genetic in nature. Epidemiological studies have found gast...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-008-2138-4
更新日期:2008-06-01 00:00:00
abstract:PURPOSE:Surgery of the inguinal canal is very common in infancy. Occasionally, injury of the bladder may occur with severe consequences. The aim of this paper was to warn young surgeons of this serious complication. METHODS:During the last 2 years, we observed four cases of bladder injury following surgery on inguinal...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-010-2707-1
更新日期:2010-12-01 00:00:00
abstract::Intestinal neuronal dysplasia is a controversial form of dysganglionosis that has been described both as an isolated disorder and in association with Hirschsprung's disease. It has been blamed for the bad outcome of bowel function in patients operated on for Hirschsprung's disease. According to various authors, intest...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-003-1035-0
更新日期:2003-12-01 00:00:00
abstract::Congenital lumbar hernia (CLH) is a rare anomaly with only 45 cases reported in the English-language literature. This paper describes nine patients with CLH treated in our unit. Unusual features included the relatively high incidence of inferior lumbar hernia, presentation at the age of 6 years in one case, and an ass...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050048
更新日期:2000-01-01 00:00:00
abstract::Accidental ingestion of corrosive substances remains a major health hazard in children. Most infants and children who ingest caustic substances present with very few symptoms or signs. Approximately 40% of caustic substance ingestions result in esophageal injury, but the optimal management of caustic esophageal burns ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-004-1331-3
更新日期:2005-02-01 00:00:00
abstract:PURPOSE:For International Neuroblastoma Staging System (INSS) stages III or IV neuroblastoma (intermediate or high risk), complete excision of the primary tumor is not always feasible. Most current studies on the treatment outcome of these patients have reported on the complete excision status. The aim of this study is...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-009-2417-8
更新日期:2009-09-01 00:00:00
abstract::Parathyroid carcinoma is unusual and its intrathyroidal variant is extremely rare. Therefore, few cases have been reported to describe a case of parathyroid carcinoma located inside the thyroid gland. The case corresponds to a 14-year-old girl who came to the office with a severe osteoarticular disease, depression, ca...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-011-2904-6
更新日期:2011-12-01 00:00:00
abstract:OBJECTIVE:Postoperative anxiety symptoms are distressing for both family and child. The aim of this study was to examine the prevalence of postoperative anxiety symptoms in children. METHODS:60 children aged 6-12 undergoing surgery were included in the study group. The study group was assessed three times in terms of ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-018-4273-x
更新日期:2018-07-01 00:00:00