qRT-PCR quantification of the biological control agent Trichoderma harzianum in peat and compost-based growing media.

Abstract:

:To ensure proper use of Trichoderma harzianum in agriculture, accurate data must be obtained in population monitoring. The effectiveness of qRT-PCR to quantify T. harzianum in different growing media was compared to the commonly used techniques of colony counting and qPCR. Results showed that plate counting and qPCR offered similar T. harzianum quantification patterns of an initial rapid increase in fungal population that decreased over time. However, data from qRT-PCR showed a population curve of active T. harzianum with a delayed onset of initial growth which then increased throughout the experiment. Results demonstrated that T. harzianum can successfully grow in these media and that qRT-PCR can offer a more distinct representation of active T. harzianum populations. Additionally, compost amended with T. harzianum exhibited a lower Fusarium oxysporum infection rate (67%) and lower percentage of fresh weight loss (11%) in comparison to amended peat (90% infection rate, 23% fresh weight loss).

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Beaulieu R,López-Mondéjar R,Tittarelli F,Ros M,Pascual JA

doi

10.1016/j.biortech.2010.09.120

subject

Has Abstract

pub_date

2011-02-01 00:00:00

pages

2793-8

issue

3

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(10)01664-0

journal_volume

102

pub_type

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