当前位置： SCI文献检索 > JOURNAL OF BONE AND MINERAL METABOLISM期刊下所有文献 > Proteomic analysis of osteoclast lipid rafts: the role of the integrity of lipid rafts on V-ATPase activity in osteoclasts.

Proteomic analysis of osteoclast lipid rafts: the role of the integrity of lipid rafts on V-ATPase activity in osteoclasts.

Abstract：

:Lipid rafts are membrane platforms for signaling molecules that regulate various cellular functions. During osteoclastogenesis, lipid rafts also have been shown to play a crucial role for cell fusion upon RANK/RANKL interaction and further activation of osteoclasts, resulting in bone resorption. To investigate proteins that localize in lipid rafts, we conducted two-dimensional (2D) gel electrophoresis followed by MALDI-TOF mass spectrometry. We identified 12 functional proteins among 34 proteins that were detectable by silver staining of the 2D gel. Among them, a subunit of vacuolar H(+)-ATPase that is involved in resorption activity of osteoclasts was identified showing an approximate molecular weight of 56.94 kDa and pI of 5.4. The solubilization of osteoclast membrane proteins followed by sucrose density gradient fractionation confirmed that V-ATPase colocalized with flotillin-1, a marker of lipid rafts, in low-density detergent-insoluble fractions. Disruption of rafts with cholesterol-sequestering agents or introduction of a dominant-negative mutant of TRAF6 impaired V-ATPase activity. These data indicate that integrity of lipid rafts regulates the activity of V-ATPase in osteoclasts, suggesting that cholesterol-lowering agents might be useful for inhibiting osteoclast-dependent bone resorption.

journal_name

J Bone Miner Metab

journal_title

Journal of bone and mineral metabolism

authors

Ryu J,Kim H,Chang EJ,Kim HJ,Lee Y,Kim HH

doi

10.1007/s00774-009-0150-y

subject

Has Abstract

pub_date

2010-07-01 00:00:00

pages

410-7

issue

4

eissn

0914-8779

issn

1435-5604

journal_volume

28

pub_type

杂志文章

相关文献

JOURNAL OF BONE AND MINERAL METABOLISM文献大全
  • Assessment of adherence to treatment of postmenopausal osteoporosis with raloxifene and/or alfacalcidol in postmenopausal Japanese women.

    abstract：:Patients who are diagnosed with osteoporosis and beginning treatment often discontinue their osteoporosis medication relatively early after the start of treatment because of their poor recognition of fracture risk and the asymptomatic nature of osteoporosis. In this study we aimed to assess adherence to treatment with...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章,随机对照试验

    doi：10.1007/s00774-009-0112-4

    authors： Gorai I,Tanaka Y,Hattori S,Iwaoki Y

    更新日期：2010-03-01 00:00:00

  • Study of the osteogenesis effect of icariside II and icaritin on canine bone marrow mesenchymal stem cells.

    abstract：:This study aimed to identify the osteogenesis effect of icariside II (ICSII) and icaritin (ICT) in vitro. Bone marrow mesenchymal stem cells (BMSCs) were treated with ICSII and ICT in order to detect the proliferation and differentiation of BMSCs, the expression of the osteogenesis-related proteins with or without ost...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s00774-017-0889-5

    authors： Luo G,Xu B,Wang W,Wu Y,Li M

    更新日期：2018-11-01 00:00:00

  • Correlation between bone mineral density and intervertebral disk degeneration in pre- and postmenopausal women.

    abstract：:The purpose of this study was to investigate the relationship between intervertebral disk degeneration and bone mass. Magnetic resonance imaging was performed to evaluate lumbar disk degeneration according to Thompson's classification (grades 1 and 2, normal disk; grades 3, 4, and 5, degenerated disk), and bone minera...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s007740300004

    authors： Nanjo Y,Morio Y,Nagashima H,Hagino H,Teshima R

    更新日期：2003-01-01 00:00:00

  • Fructooligosaccharides prevent disorders of the femoral neck following gastrectomy in growing rats.

    abstract：:Gastrectomy-evoked osteopenia in the femoral metaphysis of rats can be prevented by the consumption of fructooligosaccharides (FOS). We examined the effect of FOS on the femoral neck. Twenty-eight 5-week-old male Sprague-Dawley rats were divided into two groups, sham-operated (SH) and gastrectomized (GX). One week aft...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s00774-003-0423-9

    authors： Hirama Y,Morohashi T,Sano T,Maki K,Ohta A,Sakai N,Yamada S,Sasa R

    更新日期：2003-01-01 00:00:00

  • Changes in serum levels of biochemical markers of bone turnover during 10 years among Japanese men and women: associated factors and birth-cohort effect. The Taiji Study.

    abstract：:We aimed to clarify changes in biochemical markers of bone turnover (BTMs) over 10 years, associations with changes in bone mineral density (BMD), and birth-cohort effects in a Japanese community. We randomly selected 400 individuals (age, 40-79 years; 50 of each gender and age stratum) from a list of registered resid...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s00774-011-0266-8

    authors： Yoshimura N,Muraki S,Oka H,Kawaguchi H,Nakamura K,Akune T

    更新日期：2011-11-01 00:00:00

  • Selective cyclooxygenase-2 inhibitor prevents reduction of trabecular bone mass in collagen-induced arthritic mice in association with suppression of RANKL/OPG ratio and IL-6 mRNA expression in synovial tissues but not in bone marrow cells.

    abstract：:We performed this study to clarify whether celecoxib, a selective cyclooxygenase-2 (COX-2) inhibitor, prevents trabecular bone mass reduction by suppressing arthritis-related increase of bone resorption, and to discriminate differences in actions on bone among celecoxib, SC-58560 (a selective COX-1 inhibitor), and ind...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s00774-007-0808-2

    authors： Taketa T,Sakai A,Tanaka S,Nakai K,Menuki K,Yamane H,Tanaka K,Nakamura T

    更新日期：2008-01-01 00:00:00

  • Higher response with bone mineral density increase with monthly injectable ibandronate 1 mg compared with oral risedronate in the MOVER study.

    abstract：:We examined response to bone mineral density (BMD) gains in the MOVER study following treatment with intravenous (IV) ibandronate 1 mg/month, and investigated the characteristics of a non-responder group. At 1 year, responder rates for patients with BMD increases >0 % were similar with IV ibandronate 0.5 or 1 mg/month...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章,随机对照试验

    doi：10.1007/s00774-015-0717-8

    authors： Nakano T,Yamamoto M,Hashimoto J,Tobinai M,Yoshida S,Nakamura T

    更新日期：2016-11-01 00:00:00

  • Smoking among premenopausal women is associated with increased risk of low bone status: the JPOS Study.

    abstract：:The impact of smoking on peak bone density has not been conclusively established. We examined how smoking exposure influences bone mineral density (BMD) or the risk of low bone status in premenopausal women. We conducted a baseline survey with a representative sample of Japanese women in 1996. The effect of current an...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s00774-009-0129-8

    authors： Tamaki J,Iki M,Sato Y,Kajita E,Kagamimori S,Kagawa Y,Yoneshima H

    更新日期：2010-05-01 00:00:00

  • High calcium, phosphate and calcitriol supplementation leads to an osteocyte-like phenotype in calcified vessels and bone mineralisation defect in uremic rats.

    abstract：:A link between vascular calcification and bone anomalies has been suggested in chronic kidney disease (CKD) patients with low bone turnover disease. We investigated the vascular expression of osteocyte markers in relation to bone microarchitecture and mineralization defects in a model of low bone turnover CKD rats wit...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s00774-018-0919-y

    authors： Bisson SK,Ung RV,Picard S,Valade D,Agharazii M,Larivière R,Mac-Way F

    更新日期：2019-03-01 00:00:00

  • Prevalence of diffuse idiopathic skeletal hyperostosis (DISH) of the whole spine and its association with lumbar spondylosis and knee osteoarthritis: the ROAD study.

    abstract：:We aimed to assess the prevalence of diffuse idiopathic skeletal hyperostosis (DISH) and its association with lumbar spondylosis (LS) and knee osteoarthritis (KOA) using a population-based cohort study entitled Research on Osteoarthritis/osteoporosis Against Disability (ROAD). In the baseline ROAD study, which was per...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s00774-014-0583-9

    authors： Kagotani R,Yoshida M,Muraki S,Oka H,Hashizume H,Yamada H,Enyo Y,Nagata K,Ishimoto Y,Teraguchi M,Tanaka S,Nakamura K,Kawaguchi H,Akune T,Yoshimura N

    更新日期：2015-03-01 00:00:00

  • High prevalence of spine–femur bone mineral density discordance and comparison of vertebral fracture risk assessment using femoral neck and lumbar spine bone density in Korean patients.

    abstract：:The aim of this study was to evaluate the prevalence of spine–femur discordance, and to compare the effectiveness of femoral neck (FN) and lumbar spine (LS) bone mineral density (BMD) for estimation of the risk of vertebral fractures. Women who were evaluated with dual energy X-ray absorptiometry between January 2001 ...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s00774-013-0512-3

    authors： Seok H,Kim KJ,Kim KM,Rhee Y,Cha BS,Lim SK

    更新日期：2014-07-01 00:00:00

  • Persistence with osteoporosis medication among newly-treated osteoporotic patients.

    abstract：:Low persistence with osteoporosis medication is associated with higher fracture risk. Previous studies estimated that 1-year persistence with osteoporosis medication is low. Our aim was to study persistence with osteoporosis medication among patients with long-term follow-up (to 5 years). The InterAction Database (IAD...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s00774-013-0440-2

    authors： van Boven JF,de Boer PT,Postma MJ,Vegter S

    更新日期：2013-09-01 00:00:00

  • A possible role for leptin in normo- or hypoparathyroid uremic bone in postmenopausal dialysis women.

    abstract：:Leptin has been proposed to be a key molecule involved in energy regulation. Based on the generally acknowledged concept that a heavier person has a higher bone density, leptin is thought to be potentially involved in bone metabolism. Serum leptin, various bone markers, and bone mineral density (BMD) were studied in 5...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s007740170050

    authors： Yoneda T,Maruyama Y,Uji Y,Motomiya Y,Hashiguchi Y,Miura M,Kitajima I,Maruyama I

    更新日期：2001-01-01 00:00:00

  • Inhibition of osteoclastogenesis by prolyl hydroxylase inhibitor dimethyloxallyl glycine.

    abstract：:Studies examining the effects of hypoxia upon osteoclast biology have consistently revealed a stimulatory effect; both osteoclast differentiation and resorption activity have been shown to be enhanced in the presence of hypoxia. In the present study we examined the effects of the hypoxia mimetics dimethyloxallyl glyci...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s00774-010-0171-6

    authors： Leger AJ,Altobelli A,Mosquea LM,Belanger AJ,Song A,Cheng SH,Jiang C,Yew NS

    更新日期：2010-09-01 00:00:00

  • The role of serum osteoprotegerin and receptor-activator of nuclear factor-κB ligand in metabolic bone disease of women after obesity surgery.

    abstract：:Metabolic bone disease may appear as a complication of obesity surgery. Because an imbalance in the osteoprotegerin and receptor-activator of nuclear factor-κB ligand system may underlie osteoporosis, we aimed to study this system in humans in the metabolic bone disease occurring after obesity surgery. In this study w...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 临床试验,杂志文章

    doi：10.1007/s00774-015-0712-0

    authors： Balsa JA,Lafuente C,Gómez-Martín JM,Galindo J,Peromingo R,García-Moreno F,Rodriguez-Velasco G,Martínez-Botas J,Gómez-Coronado D,Escobar-Morreale HF,Botella-Carretero JI

    更新日期：2016-11-01 00:00:00

  • Number and severity of prevalent vertebral fractures and the risk of subsequent vertebral fractures in Japanese women with osteoporosis: results from the minodronate trial.

    abstract：:The aim was to evaluate the risk of new vertebral fractures with the increasing number and severity of prevalent vertebral fractures in women who received placebo or minodronate in a post hoc analysis of a 2-year randomized, double-blind, placebo-controlled study. The subjects were women aged 55-80 years old with 1-5 ...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章,随机对照试验

    doi：10.1007/s00774-013-0439-8

    authors： Hagino H,Shiraki M,Fukunaga M,Nakano T,Takaoka K,Ohashi Y,Nakamura T,Matsumoto T

    更新日期：2013-09-01 00:00:00

  • Menin and bone metabolism.

    abstract：:Menin, a product of the MEN1 gene, is related to the ontogeny of several cancers such as MEN1 and sporadic endocrine tumors, although it is considered to be a tumor suppressor. Many proteins interact with menin, and it is involved in various biological functions in several tissues. Menin plays some physiological and p...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章,评审

    doi：10.1007/s00774-012-0355-3

    authors： Kaji H

    更新日期：2012-07-01 00:00:00

  • Incidence of osteonecrosis of the jaw in Japanese osteoporosis patients taking minodronic acid.

    abstract：:Osteonecrosis of the jaw (ONJ) associated with bisphosphonate therapy is a rare but severe side effect in osteoporosis patients. Recently, the number of osteoporosis patients with ONJ has dramatically increased in Japan. This has contributed to an increase in the number of patients avoiding extractions. However, there...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章,多中心研究,随机对照试验

    doi：10.1007/s00774-019-00990-5

    authors： Taguchi A,Uemura Y,Imai T,Tanaka S,Ohta H,Nakamura T,Orimo H,Sugimoto T,Soen S,Shiraki M,Adequate Treatment of Osteoporosis (A-TOP) research group.

    更新日期：2019-09-01 00:00:00

  • Identification of a novel frameshift mutation (383insT) in the RUNX2 (PEBP2 alpha/CBFA1/AML3) gene in a Japanese patient with cleidocranial dysplasia.

    abstract：:Cleidocranial dysplasia (CCD) is an autosomal dominant disorder due to mutations in runt-related gene 2 (RUNX2)/polyomavirus enhancer-binding protein 2alphaA (PEBP2alphaA)/core-binding factor A1 (CBFA1)/acute myeloid leukemia 3 (AML3). To investigate the RUNX2 mutations in a Japanese patient with classic CCD, we analy...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s007740170030

    authors： Goseki-Sone M,Orimo H,Watanabe A,Hamatani R,Yokozeki M,Ohyama K,Kuroda T,Watanabe H,Miyazaki H,Shimada T,Oida S

    更新日期：2001-01-01 00:00:00

  • Collagen-related abnormalities, reduction in bone quality, and effects of menatetrenone in rats with a congenital ascorbic acid deficiency.

    abstract：:In this study, we focused on collagen metabolism as a factor involved in menatetrenone (MK-4)-related improvement in bone quality. Using rats with a congenital ascorbic acid (AA) deficiency, osteogenic disorder Shionogi (ODS) rats, we established a model in which abnormal collagen metabolism reduced bone mechanical pr...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s00774-009-0069-3

    authors： Hara K,Akiyama Y

    更新日期：2009-01-01 00:00:00

  • Identification of oxytetracycline as a chondrogenic compound using a cell-based screening system.

    abstract：:To effectively treat degenerative joint diseases including osteoarthritis (OA), small chemical compounds need to be developed that can potently induce chondrogenic differentiation without promoting terminal differentiation. For this purpose, we screened natural and synthetic compound libraries using a Col2GFP-ATDC5 sy...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s00774-010-0179-y

    authors： Hojo H,Yano F,Ohba S,Igawa K,Nakajima K,Komiyama Y,Kan A,Ikeda T,Yonezawa T,Woo JT,Takato T,Nakamura K,Kawaguchi H,Chung UI

    更新日期：2010-11-01 00:00:00

  • Visfatin is a positive predictor of bone mineral density in young survivors of acute lymphocytic leukemia.

    abstract：:Bone mass acquisition may be compromised in survivors of childhood acute lymphocytic leukemia due to various factors, including adiposity. Fat accumulation can affect bone through the direct effect of adipokines or indirectly through the state of chronic inflammation. The aim of this study was to evaluate the effect o...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章,多中心研究,随机对照试验

    doi：10.1007/s00774-015-0728-5

    authors： Siviero-Miachon AA,Spinola-Castro AM,de Martino Lee ML,Calixto AR,Geloneze B,Lazaretti-Castro M,Guerra-Junior G

    更新日期：2017-01-01 00:00:00

  • Nontraumatic lumbar vertebral compression fracture as a risk factor for femoral neck fractures in involutional osteoporotic patients.

    abstract：:To screen a potential risk factor for femoral neck fracture, we characterized lumbar vertebral fractures in 120 patients with femoral neck fractures (19 men, 101 women; mean age, 78.7 years) by investigating the frequency of patients with lumbar vertebral fracture, the number of vertebral fractures per patient, and th...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s007740050085

    authors： Kinoshita T,Ebara S,Kamimura M,Tateiwa Y,Itoh H,Yuzawa Y,Takahashi J,Takaoka K

    更新日期：1999-01-01 00:00:00

  • Identification of a progenitor cell population destined to form fracture fibrocartilage callus in Dickkopf-related protein 3-green fluorescent protein reporter mice.

    abstract：:Fracture healing is a complex biological process involving the proliferation of mesenchymal progenitor cells, and chondrogenic, osteogenic, and angiogenic differentiation. The mechanisms underlying the proliferation and differentiation of mesenchymal progenitor cells remain unclear. Here, we demonstrate Dickkopf-relat...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s00774-015-0711-1

    authors： Mori Y,Adams D,Hagiwara Y,Yoshida R,Kamimura M,Itoi E,Rowe DW

    更新日期：2016-11-01 00:00:00

  • Hypophosphatemic rickets accompanying McCune-Albright syndrome: evidence that a humoral factor causes hypophosphatemia.

    abstract：:McCune-Albright syndrome (MAS) is sometimes complicated by hypophosphatemia. However, it remains unclear whether a humoral factor is associated with the cause of hypophosphatemia. We isolated cells with mutations of the Gsalpha gene from fibrous bone dysplasia tissues of two MAS patients (MAS cells). Severe combined i...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s007740170012

    authors： Yamamoto T,Miyamoto KI,Ozono K,Taketani Y,Katai K,Miyauchi A,Shima M,Yoshikawa H,Yoh K,Takeda E,Okada S

    更新日期：2001-01-01 00:00:00

  • Biochemical bone turnover markers are useful tools to assess changes in bone metabolism in marmosets.

    abstract：:This study was designed to investigate whether biochemical markers of bone resorption and formation could be determined in the serum and urine of marmosets (Callithrix jacchus), using standard laboratory chemistry methods and commercially available human kits. Consequently, the findings from this study will indicate w...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s00774-003-0469-8

    authors： Angeliewa A,Budde M,Schlachter M,Hoyle NR,Bauss F

    更新日期：2004-01-01 00:00:00

  • Lactoferrin immuno-expression in human normal and neoplastic bone tissue.

    abstract：:Lactoferrin (Lf) expression was investigated by using a Lf monoclonal antibody in 50 formalin-fixed and paraffin-embedded human bone tumours [10 giant cell tumours (GCTs), 7 osteoid osteomas, 6 ossifying fibromas, 19 enchondromas, 2 chondroblastomas, 2 chondrosarcomas, 2 chondroblastic osteosarcomas, 1 myeloma and 1 a...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s00774-009-0044-z

    authors： Ieni A,Barresi V,Grosso M,Rosa MA,Tuccari G

    更新日期：2009-01-01 00:00:00

  • Eldecalcitol reduces the risk of severe vertebral fractures and improves the health-related quality of life in patients with osteoporosis.

    abstract：:Eldecalcitol reduces the risk of vertebral fractures in comparison to alfacalcidol in osteoporotic patients under vitamin D repletion. The aim of this study was to evaluate the effects of eldecalcitol on the spinal location of incident vertebral fractures, the severity of the fractures, and the changes in health-relat...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章,随机对照试验

    doi：10.1007/s00774-012-0397-6

    authors： Hagino H,Takano T,Fukunaga M,Shiraki M,Nakamura T,Matsumoto T

    更新日期：2013-03-01 00:00:00

  • Adult hypophosphatasia with painful periarticular calcification treated with surgical resection.

    abstract：:Hypophosphatasia is a rare inherited disorder characterized by a low serum alkaline phosphatase (ALP) activity and defective bone mineralization. Adult hypophosphatasia typically manifests in middle-age as a result of osteomalacia with recurrent stress fractures of the lower limb. However, considerable variation occur...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s00774-011-0338-9

    authors： Iida K,Fukushi J,Fujiwara T,Oda Y,Iwamoto Y

    更新日期：2012-11-01 00:00:00

  • Tracking of appendicular bone mineral density for 6 years including the pubertal growth spurt: Japanese Population-based Osteoporosis kids cohort study.

    abstract：:Bone development up to early adulthood plays an important role in determining the risk of osteoporosis later in life. However, bone development in children has not been fully documented by longitudinal studies in Japanese children. The purpose of this study is to determine the degree of tracking of areal bone mineral ...

    journal_title：Journal of bone and mineral metabolism

    pub_type： 杂志文章

    doi：10.1007/s00774-010-0213-0

    authors： Fujita Y,Iki M,Ikeda Y,Morita A,Matsukura T,Nishino H,Yamagami T,Kagamimori S,Kagawa Y,Yoneshima H

    更新日期：2011-03-01 00:00:00