Abstract：
:Hepatic cell transplantation consists of grafting already differentiated cells such as hepatocytes. Human hepatocytes are viable and functionally active. Liver cell transplantation is carried out by means of a 3-step method: isolation of hepatocytes from donor liver rejected for orthotopic transplantation, preparing a cell suspension for infusion and, finally, hepatocytes are implanted into the recipient. There are established protocols for the isolation of human hepatocytes from unused segments of donor livers, based on collagenase digestion of cannulated liver tissue at 37 degrees C. The hepatocytes can be used fresh or cryopreserved. Cryopreservation of isolated human hepatocytes would then be available for planned use. In cell transplant, the important aspects are: infusion route, number of cells, number of infusions and viability of the cells. The cells are infused into the patient through a catheter inserted via portal vein or splenic artery. Liver cell transplantation allows liver tissue to be used that would, otherwise, be discarded, enabling multiple patients to be treated with hepatocytes from a single tissue donor.
journal_name
Cir Espjournal_title
Cirugia espanolaauthors
Pareja E,Martínez A,Cortés M,Bonora A,Moya A,Sanjuán F,Gómez-Lechón MJ,Mir Jdoi
10.1016/j.ciresp.2009.10.002subject
Has Abstractpub_date
2010-03-01 00:00:00pages
139-47issue
3eissn
0009-739Xissn
1578-147Xpii
S0009-739X(09)00523-5journal_volume
87pub_type
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