Abstract：
:Increasingly, combinatorial libraries are used to screen large numbers of complex chemically- and physically-patterned polymers against cell response. There is a strong need to extract meaningful relationships between cell function and material surface features from these experiments. A novel high-throughput cell-material screening strategy, based upon local cell-feature analysis (LCFA) was applied to screen osteoblast proliferation behavior on combinatorial libraries of phase-separated PLGA and PCL. Traditional factor importance analysis, which uses summary statistical inference to identify significant variables, indicated that one controlling material surface feature was PCL diameter. However, the summary statistic analysis was unable to uncover more subtle relationships. The LCFA method, based on histograms of distances between cells and microstructures, was able to identify non-linear, discrete relationships between proliferation, PCL diameter, and cell-PCL distance. LCFA provides an advantage in that a distribution function is not assumed, but rather is developed from the data. Using these results, we propose a model for classifying the material-microstructure interactions, in which small PCL islands far from the cell nucleus act as holders for attachment and large islands close to cells act to shape the cell.
journal_name
Comb Chem High Throughput Screenjournal_title
Combinatorial chemistry & high throughput screeningauthors
Su J,Meredith JCdoi
10.2174/138620709788681880subject
Has Abstractpub_date
2009-07-01 00:00:00pages
626-33issue
6eissn
1386-2073issn
1875-5402journal_volume
12pub_type
杂志文章abstract：:Recent studies have suggested that both constitutive androstane receptor (CAR) and pregnane X-receptor (PXR) are involved in the induction of rat liver microsomal cytochrome P-450 (CYP) 2B and 3A through a mechanism called cross-talk. In this study we intend to determine if a PXR-reporter gene assay could be used for ...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207054020822
更新日期：2005-06-01 00:00:00
abstract：:Two approaches were utilized to increase the throughput of pulsed ultrafiltration assays of ligand binding to human serum albumin, reducing the volume of the ultrafiltration chamber and combining pulsed ultrafiltration with high performance liquid chromatography-atmospheric pressure chemical ionization mass spectromet...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:Building accurate quantitative structure-activity relationships (QSAR) is important in drug design, environmental modeling, toxicology, and chemical property prediction. QSAR methods can be utilized to solve mainly two types of problems viz., pattern recognition, (or classification) where output is discrete (i.e. clas...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：10.2174/138620709788167908
更新日期：2009-05-01 00:00:00
abstract：:We describe a high-throughput, quantitative technology for fast identification of all different clones present in selectively enriched phage surface-displayed cDNA libraries. The strategy is based on a combination of phage display and high-density arrays. To demonstrate the utility of the method cDNAs of Aspergillus f...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207033329751
更新日期：2003-03-01 00:00:00
abstract：:In this review various technologies and approaches for the utilization of mass spectrometry in high-throughput analyses are discussed. The use of quadrupole-based mass spectrometry in the screening of chemical libraries against enzymatic targets for the identification of inhibitors and/or activators is highlighted. Th...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：10.2174/138620709789104924
更新日期：2009-09-01 00:00:00
abstract：:Chemical inhibitors have had a profound impact on many diverse fields of biology. The goal of chemical genetics is to use small molecules to perturb biological systems in a manner conceptually similar to traditional genetics. Key to the advancement of the chemical genetic paradigm is the further development of tools a...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：10.2174/1386207043328391
更新日期：2004-11-01 00:00:00
abstract：:P-glycoprotein (P-gp) is well known to cause multidrug resistance (MDR) in cancer cells. This MDR leads to cancer recurrence which is a major obstacle in cancer treatment. High P-gp expression has been observed in the population of cancer stem cells (CSCs) having self-renewal potential. Early detection and inhibition ...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 历史文章,杂志文章
doi：10.2174/1386207319666160517115158
更新日期：2016-01-01 00:00:00
abstract：AIM AND OBJECTIVE:Lung cancer is a highly heterogeneous cancer, due to the significant differences in molecular levels, resulting in different clinical manifestations of lung cancer patients there is a big difference. Including disease characterization, drug response, the risk of recurrence, survival, etc. Method:Clin...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207322666190125151624
更新日期：2018-01-01 00:00:00
abstract：AIM AND OBJECTIVE:Avicularin has been found to inhibit the proliferation of HepG-2 cells in vitro in the screening of our laboratory. We intended to explain the molecular mechanism of this effect. Therefore, the combined methods of reverse molecular docking and network pharmacology were used in order to illuminate the ...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207322666190206163409
更新日期：2019-01-01 00:00:00
abstract：:NMR has proven to be a valuable tool for identifying small molecule drug leads that serve as starting points for lead optimization programs. In addition, NMR screening can also be applied during lead optimization in order to improve the pharmacokinetic properties of a compound. In this paper we review the NMR methods ...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：10.2174/1386207023329941
更新日期：2002-12-01 00:00:00
abstract：BACKGROUND:Thiazolidine compounds are known to show interesting pharmacological activity. In particular, they are used as antiseizure, fungicidal, anti-bacterial, antitubercular, antiinflammatory, antiamoebic, anti-diabetic and local anesthetic agents. Some of these compounds have also shown antiparkinsonism, antioxida...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207321666180124094055
更新日期：2018-01-01 00:00:00
abstract：AIM AND OBJECTIVE:Flap endonuclease-1 (FEN1) plays a central role in DNA replication and DNA damage repair process. In mammals, FEN1 functional sites variation is related to cancer and chronic inflammation, and supports the role of FEN1 as a tumor suppressor. However, FEN1 is overexpressed in multiple types of cancer c...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207322666190704095602
更新日期：2019-01-01 00:00:00
abstract：:Due to the numerous challenges in hit identification from random RNAi screening, we have examined current practices with a discovery of a variety of methodologies employed and published in many reports; majority of them, unfortunately, do not address the minimum associated criteria for hit nomination, as this could po...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/138620712803519671
更新日期：2012-11-01 00:00:00
abstract：:In recent years, combinatorial libraries have become a major tool for drug discovery and drug development. Along the way, one potential use of combinatorial chemistry libraries almost been neglected: the basic study of intermolecular interactions. Especially "one-bead-one-structure" libraries can be a powerful means f...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：
更新日期：1998-10-01 00:00:00
abstract：:As technologies for high throughput and high content screening continue to evolve, new platforms for quantitative cellular imaging will play an increasingly important role in identifying and profiling lead compounds. To gain insight into the effects of a compound on cell morphology or intracellular events, it is neces...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：10.2174/138620709789383204
更新日期：2009-11-01 00:00:00
abstract：:The appropriate selection of initial receptor structure has been the 'cornerstone' or foundation of successful structure-based virtual screening (SBVS), and plagued the structure-based design with a significantly practical problem to determine the major physiological states or important transition states of receptors ...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207323666201110150140
更新日期：2020-11-10 00:00:00
abstract：BACKGROUND:A set of antibiotic fluoroquinolones with confirmed antimicrobial activity were analyzed with the use of two types of quantum chemical calculation methods and quantitative structure-activity relationships (QSAR). OBJECTIVE:The purpose of this study was to demonstrate the common and differentiating character...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207321666180827105856
更新日期：2018-01-01 00:00:00
abstract：:A general procedure for the synthesis of a large variety of compounds comprising an alpha, beta,-unsaturated carbonyl functionality was developed. The use of one-pot cascade synthesis with (triphenylphosphora-nylidene)ethenone as a versatile reagent for various formations including heterocycles of different ring sizes...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207023330066
更新日期：2002-11-01 00:00:00
abstract：:Fingerprint representations of molecular structure and properties were among the first computational tools for similarity searching and are widely applied to this date, which is in part due to their computational efficiency and ease-of-use. Moreover, despite their simplicity, 2D molecular fingerprints have been surpri...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/138620710790980487
更新日期：2010-03-01 00:00:00
abstract：:For years, the physicochemical properties of drug candidates have been used to predict their in vivo pharmacokinetic behaviors. Several theories and empirical correlations have been established by various researchers with the overall goal of expediting the drug candidate selection process, with greater confidence and ...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/138620709787581693
更新日期：2009-03-01 00:00:00
abstract：:The recent success of the human genome project and the continued accomplishment in obtaining DNA sequences for a vast array of organisms is providing an unprecedented wealth of information. Nevertheless, an abundance of the proteome contains hypothetical proteins or proteins of unknown function, where high throughput ...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：10.2174/138620707782507331
更新日期：2007-09-01 00:00:00
abstract：:A new and simple high-performance liquid chromatography method was developed and validated for the simultaneous determination of retinol, retinyl palmitate, β-carotene, α-tocopherol and vitamin C in rat serum treated with Plantago Major L. and 7,12 dimethylbenz[a]anthracene. High-performance liquid chromatography anal...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：
更新日期：2013-02-01 00:00:00
abstract：:Chromosome analysis has been a cornerstone both for the identification of genetic defects that predispose to a variety of genetic syndromes as well as for the analysis of cancer progression. The relatively low resolution of metaphase chromosomes, however, only allows characterization of major genetic events which are ...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：10.2174/1386207043328481
更新日期：2004-09-01 00:00:00
abstract：:Microwave-assisted Suzuki-Miyaura and Stille cross-coupling reactions for the synthesis of highly electron-rich and diversely functionalized biaryl intermediates are presented. Microwave-irradiation has been demonstrated to be a very powerful tool for performing difficult transition metal-catalyzed cross-coupling reac...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/138620707783018513
更新日期：2007-11-01 00:00:00
abstract：:Widely diverse biological queries are now routinely analyzed on the various optical platforms: laser line scanners, nonconfocal imagers and confocal imagers. These analyses may be performed to query a limited number of samples or range to include the evaluation of a million samples as is the goal of many screening dep...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：10.2174/138620709789383231
更新日期：2009-11-01 00:00:00
abstract：:The Emory Chemical Biology Discovery Center (ECBDC) aims to accelerate high throughput biology and translation of biomedical research discoveries into therapeutic targets and future medicines by providing high throughput research platforms to scientific collaborators worldwide. ECBDC research is focused at the interfa...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207317666140109125415
更新日期：2014-03-01 00:00:00
abstract：BACKGROUND:Clinical outcomes after rotator cuff repair associated with diabetes mellitus(DM) are generally favorable, but no study has attempted to establish the influence of DM on outcomes after rotator cuff repair. PURPOSE:To conduct a meta-analysis of clinical studies evaluating patient outcomes between people with...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： meta分析
doi：10.2174/1386207323666201020114034
更新日期：2020-10-20 00:00:00
abstract：:Structural genomics, structure-based analysis of gene products, has so far mainly concentrated on soluble proteins because of their less demanding requirements for overexpression, purification and crystallisation compared to membrane proteins. This so-called "low-hanging fruit" approach has generated more than 25,000 ...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：10.2174/1386207043328634
更新日期：2004-08-01 00:00:00
abstract：:Mitochondria are the center of metabolism and the critical role of aberrant mitochondrial fission in the onset and progression of a wide range of human diseases such as neurodegenerative disorders, cardiovascular disease, ischemic stroke and diabetes, is slowly becoming recognized. Under physiological conditions, mito...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207311316010044
更新日期：2014-01-01 00:00:00
abstract：AIM AND OBJECTIVE:In the past decade, the drug design technologies have been improved enormously. The computer-aided drug design (CADD) has played an important role in analysis and prediction in drug development, which makes the procedure more economical and efficient. However, computation with big data, such as ZINC c...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207321666180102120641
更新日期：2018-01-01 00:00:00
