当前位置： SCI文献检索 > COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING期刊下所有文献 > A simple method for analyzing actives in random RNAi screens: introducing the "H Score" for hit nomination & gene prioritization.

A simple method for analyzing actives in random RNAi screens: introducing the "H Score" for hit nomination & gene prioritization.

Abstract：

:Due to the numerous challenges in hit identification from random RNAi screening, we have examined current practices with a discovery of a variety of methodologies employed and published in many reports; majority of them, unfortunately, do not address the minimum associated criteria for hit nomination, as this could potentially have been the cause or may well be the explanation as to the lack of confirmation and follow up studies, currently facing the RNAi field. Overall, we find that these criteria or parameters are not well defined, in most cases arbitrary in nature, and hence rendering it extremely difficult to judge the quality of and confidence in nominated hits across published studies. For this purpose, we have developed a simple method to score actives independent of assay readout; and provide, for the first time, a homogenous platform enabling cross-comparison of active gene lists resulting from different RNAi screening technologies. Here, we report on our recently developed method dedicated to RNAi data output analysis referred to as the BDA method applicable to both arrayed and pooled RNAi technologies; wherein the concerns pertaining to inconsistent hit nomination and off-target silencing in conjugation with minimal activity criteria to identify a high value target are addressed. In this report, a combined hit rate per gene, called "H score", is introduced and defined. The H score provides a very useful tool for stringent active gene nomination, gene list comparison across multiple studies, prioritization of hits, and evaluation of the quality of the nominated gene hits.

journal_name

Comb Chem High Throughput Screen

journal_title

Combinatorial chemistry & high throughput screening

authors

Bhinder B,Djaballah H

doi

10.2174/138620712803519671

subject

Has Abstract

pub_date

2012-11-01 00:00:00

pages

686-704

issue

9

eissn

1386-2073

issn

1875-5402

pii

CCHTS-EPUB-20120828-9

journal_volume

15

pub_type

杂志文章

相关文献

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING文献大全
  • The Application of High-Throughput Technologies in Precision Diagnosis and Personalized Therapy (Pt III).

    abstract：: ...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 社论

    doi：10.2174/138620732204190724103437

    authors： Huang T

    更新日期：2019-01-01 00:00:00

  • In Vitro Antifungal Screening of Argentine Native or Naturalized Plants against the Phytopathogen Monilinia fructicola.

    abstract：BACKGROUND:One of the main problems that fruit health has gone through in recent years is the difficult eradication of their fungal pathogens during crops or the post-harvest stage. This concerns the whole world because it represents huge production losses, fruit export restrictions and consumer distrust. Consequently,...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/1386207324666210121113648

    authors： Stegmayer MI,Fernández LN,Álvarez NH,Seimandi GM,Reutemann AG,Derita MG

    更新日期：2021-01-20 00:00:00

  • Assays of ligand-human serum albumin binding using pulsed ultrafiltration and liquid chromatography-mass spectrometry.

    abstract：:Two approaches were utilized to increase the throughput of pulsed ultrafiltration assays of ligand binding to human serum albumin, reducing the volume of the ultrafiltration chamber and combining pulsed ultrafiltration with high performance liquid chromatography-atmospheric pressure chemical ionization mass spectromet...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：

    authors： Gu C,Nikolic D,Lai J,Xu X,van Breemen RB

    更新日期：1999-12-01 00:00:00

  • Network Pharmacology and Reverse Molecular Docking-Based Prediction of the Molecular Targets and Pathways for Avicularin Against Cancer.

    abstract：AIM AND OBJECTIVE:Avicularin has been found to inhibit the proliferation of HepG-2 cells in vitro in the screening of our laboratory. We intended to explain the molecular mechanism of this effect. Therefore, the combined methods of reverse molecular docking and network pharmacology were used in order to illuminate the ...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/1386207322666190206163409

    authors： Duan C,Li Y,Dong X,Xu W,Ma Y

    更新日期：2019-01-01 00:00:00

  • Screening drugs for metabolic stability using pulsed ultrafiltration mass spectrometry.

    abstract：:A pulsed ultrafiltration-mass spectrometric screening method has been developed to evaluate the metabolic stability of drugs. Pooled human liver microsomes containing cytochrome P450 enzymes were trapped by an ultrafiltration membrane in a stirred flow-through chamber, and eight beta-blocker drugs including acebutolol...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/1386207023330633

    authors： Geun Shin Y,Bolton JL,van Breemen RB

    更新日期：2002-02-01 00:00:00

  • Synthesis and Characterization of a Novel Ionic Liquid Based on N,N,N,N-tetramethylethylenediamine and its Application in the Synthesis of 1,8-dioxo-octahydro Xanthenes.

    abstract：AIM AND OBJECTIVE:In this work, we synthesized and characterized a novel Brönsted acidic ionic liquid from the reaction of N, N, N', N'-tetramethylethylenediamine with chlorosulfonic acid and explored its catalytic activity in 1, 8-dioxo-octahydroxanthenes synthesis. MATERIALS AND METHODS:Dimedone, aryl aldehydes, and...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/1386207321666180828092546

    authors： Piralghar ZA,Hashemi MM,Ezabadi A

    更新日期：2018-01-01 00:00:00

  • Evaluation of drug transporter interactions in drug discovery and development.

    abstract：:Drug transporters play an important role in the absorption, distribution, excretion and toxicity of both endogenous and exogenous compounds. Transporters may act as physiological 'gatekeepers' in the regulation of the pharmacological and/or toxicological effects of drugs by limiting distribution to tissues responsible...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章,评审

    doi：10.2174/138620710790596772

    authors： Lai Y,Sampson KE,Stevens JC

    更新日期：2010-02-01 00:00:00

  • A green and efficient protocol for the synthesis of quinoxaline, benzoxazole and benzimidazole derivatives using heteropolyanion-based ionic liquids: as a recyclable solid catalyst.

    abstract：:In this paper, we introduce two nonconventional ionic liquid compounds which are composed of propane sulfonate functionalized organic cations and heteropolyanions as green solid acid catalysts for the highly efficient and green synthesis of 2,3-disubstitutedquinoxaline derivatives. These ionic liquids are in the solid...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/1386207311316080004

    authors： Vahdat SM,Baghery S

    更新日期：2013-09-01 00:00:00

  • Efficient One-Pot Synthesis of Thiazol-2-imine Derivatives through Regioselective Reaction Between Primary Amines, Phenylisothiocyanate, and α-Chloroacetaldehyde.

    abstract：AIM AND OBJECTIVE:Thiazol-2-imine derivatives are interested for their pharmaceutical and biologic activities. A literature survey reveals that there have been no any reports on the synthesis of thiazol-2-imine derivatives without substituents in position C-4 and C-5 via one-pot reaction. Herein we report an efficient ...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/1568009617666161107093942

    authors： Nasiri F,Sabahi-Agabager L

    更新日期：2017-01-01 00:00:00

  • High throughput screening and structure-activity relationship study of potential α2A-adrenoceptor agonists by LANCETM cAMP assay.

    abstract：:G protein-coupled receptors (GPCRs) are signaling molecules with a wide variety of skills. Members of this large family of membrane protein have been shown to regulate the activities of the different signaling pathways of the ligand specific manner. α2-adrenoceptors (α2-ARs) are one of the GPCRs and the stimulation of...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/1386207311316070003

    authors： Yang H,He L,Yan M,He JG,Yu T

    更新日期：2013-06-28 00:00:00

  • Enantiomeric resolution of ibuprofen and flurbiprofen in human plasma by SPE-chiral HPLC methods.

    abstract：:Chiral analysis of profens in human plasma is an important area of research due to different pharmaceutical activities of their enantiomers. The solid phase extraction of ibuprofen and flurbiprofen from human plasma was carried out on C18 cartridges by using phosphate buffer (50 mM, pH 6.0) followed by elution with me...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/138620712800563882

    authors： Ali I,Hussain I,Saleem K,Aboul-Enein HY

    更新日期：2012-07-01 00:00:00

  • In silico screening of antifolate based novel inhibitors from Brucea mollis Wall. ex kurz against quadruple mutant drug resistant PfDHFR.

    abstract：:Plasmodium falciparum is the most lethal form of the genus Plasmodium which causes malaria, a 'disease of antiquity'. Globally it affects the health and socio-economic development of a large population especially in Sub-Saharan Africa and Southeast Asia. The Plasmodium falciparum dihydrofolate reductase-thymidylate sy...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/1386207317666140521153305

    authors： Borkakoty B,Sarma K,Parida P,Prakash A,Mohapatra PK,Mahanta J

    更新日期：2014-01-01 00:00:00

  • Identification of Novel Antagonists for Rab38 Protein by Homology Modeling and Virtual Screening.

    abstract：BACKGROUND:The Rab family proteins are involved in membrane trafficking, cell growth and differentiation. Rab38 is implicated in the biogenesis of melanosomes that help in the synthesis, storage and transport of melanin pigments. The Rab38 protein is overexpressed at the RNA level in melanoma cancer. AIM AND OBJECTIVE...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/1386207319666161026153237

    authors： Abdelmonsef AH,Dulapalli R,Dasari T,Padmarao LS,Mukkera T,Vuruputuri U

    更新日期：2016-01-01 00:00:00

  • Recent developments of in silico predictions of oral bioavailability.

    abstract：:Unfavorable oral bioavailability is an important reason accounting for the failure of the drug candidates. Considering the lack of in vitro high-throughput screening assay for oral bioavailability, it is critical to develop in silico models for early predictions of oral bioavailability. In this review, we summarize pr...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章,评审

    doi：10.2174/138620711795508368

    authors： Zhu J,Wang J,Yu H,Li Y,Hou T

    更新日期：2011-06-01 00:00:00

  • Selective molecularly imprinted polymer obtained from a combinatorial library for the extraction of bisphenol A.

    abstract：:In the present work, an analytical methodology based on molecularly imprinted solid-phase extraction (MISPE) has been developed for the determination of bisphenol A (BPA) in environmental and food samples. In order to select the optimum material, a combinatorial library of molecularly imprinted polymers in small-scale...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/138620706779026024

    authors： Martin-Esteban A,Tadeo JL

    更新日期：2006-12-01 00:00:00

  • On the Spacer Group Effect on Critical Micelle Concentration of Cationic Gemini Surfactants Using Molecular Connectivity Indices.

    abstract：:The important factor which differentiates gemini surfactants from conventional monomeric surfactants is the spacer group. The molecular connectivity method was used to study the effect of the spacer group on critical micelle concentration of cationic gemini surfactants. Two models were derived employing only Kier and ...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/1386207319666160504095717

    authors： Mozrzymas A

    更新日期：2016-01-01 00:00:00

  • Chemoinformatics Approach for Building Molecular Networks from Marine Organisms.

    abstract：:Natural products obtained from marine sources are considered to be a rich and diverse source of potential drugs. In the present work we demonstrate the use of chemoinformatics approach for the design of new molecules inspired by molecules from marine organisms. Accordingly we have assimilated information from two majo...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/1386207318666150703112950

    authors： Karthikeyan M,Nimje D,Pahujani R,Tyagi K,Bapat S,Vyas R,Pillai Padmakumar K

    更新日期：2015-01-01 00:00:00

  • Ligand Based-Pharmacophore Modeling and Extended Bi oactivity Prediction for Salinosporamide A, B and C from Marine Actino mycetes Salinispora tropica.

    abstract：AIM AND OBJECTIVE:Actinomycetes produce structurally unique secondary metabolites with pharmaceutically essential bioactivities. Salinispora, an obligate marine actinomycete, produces structurally varied and unique secondary metabolites. There is plenty of scope for development of drugs from the novel compounds isolate...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/1386207319666161215154128

    authors： Dineshkumar K,Vasudevan A,Hopper W

    更新日期：2017-01-01 00:00:00

  • Cyclopeptide analogs for generating new molecular and 3D diversity.

    abstract：:Cyclic peptides have been often utilized as metabolically stable, conformationally restricted mimics of different kinds of biologically active peptides, including peptide antibiotics, endogenous opioid peptides, integrin inhibitors, peptide hormones, anticancer peptides, and so on. And in particular, cyclic compounds ...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/138620709789824754

    authors： Gentilucci L,Cardillo G,Tolomelli A,Squassabia F,De Marco R,Chiriano G

    更新日期：2009-12-01 00:00:00

  • In silico identification of potential dynamin-related protein 1 antagonists: implications for diseases involving mitochondrial dysfunction.

    abstract：:Mitochondria are the center of metabolism and the critical role of aberrant mitochondrial fission in the onset and progression of a wide range of human diseases such as neurodegenerative disorders, cardiovascular disease, ischemic stroke and diabetes, is slowly becoming recognized. Under physiological conditions, mito...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/1386207311316010044

    authors： Hanumanthappa P,Rajanikant GK

    更新日期：2014-01-01 00:00:00

  • "Whole cell"--matrix-assisted laser desorption ionization-time of flight-mass spectrometry, an emerging technique for efficient screening of biocombinatorial libraries of natural compounds-present state of research.

    abstract：:Whole Cell-matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) is an emerging sensitive technique for rapid typing of microorganisms, efficient screening of biocombinatorial libraries of natural compounds and the analysis of complex biological samples, as whole cells, subcellula...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章,评审

    doi：10.2174/138620703106298725

    authors： Vater J,Gao X,Hitzeroth G,Wilde C,Franke P

    更新日期：2003-09-01 00:00:00

  • Bioactivity-Guided Isolation of Antiproliferative Sesquiterpene Lactones from Centaurea solstitialis L. ssp. solstitialis.

    abstract：:Centaurea solstitialis L. ssp. solstitialis (CSS) has been used as medicine for various diseases. In this work, root, stem and flower parts of the plant were separately extracted with methanol to execute bioassay-guided isolation. Antiproliferative activities of each extracts on C6 cells (Rat Brain tumor cells) and He...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/1386207319666151203002117

    authors： Erenler R,Sen O,Yaglioglu AS,Demirtas I

    更新日期：2016-01-01 00:00:00

  • Gene expression analysis for high throughput screening applications.

    abstract：:To meet growing needs for high throughput gene expression profiling, we established a new automated high throughput TaqMan RT-PCR method for quantitative mRNA expression analysis. In this method, the Allegro( trade mark ) (Zymark) system conducts all sample tracking and liquid handling steps, and ABI PRISM 7900 HT (Ap...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/138620704773120810

    authors： Pinhasov A,Mei J,Amaratunga D,Amato FA,Lu H,Kauffman J,Xin H,Brenneman DE,Johnson DL,Andrade-Gordon P,Ilyin SE

    更新日期：2004-03-01 00:00:00

  • "In silico" design of potential anti-HIV actives using fragment descriptors.

    abstract：:Substructural Molecular Fragments (SMF) method was applied for computer-aided design of new compounds potentially possessing high anti-HIV activities: tetrahydroimidazobenzodiazepinone (TIBO) derivatives and 1-[2-hydroxyethoxy)methyl]-6-(phenylthio)thymine (HEPT) derivatives. Using available experimental data, the SMF...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/1386207054546513

    authors： Varnek A,Solov'ev VP

    更新日期：2005-08-01 00:00:00

  • Synthesis, Antitumor Activity and Molecular Docking Studies on Seven Novel Thiazacridine Derivatives.

    abstract：AIM AND OBJECTIVE:In the last decades, cancer has become a major problem in public health all around the globe. Chimeric chemical structures have been established as an important trend on medicinal chemistry in the last years. Thiazacridines are hybrid molecules composed of a thiazolidine and acridine nucleus, both pha...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/1386207323666200319105239

    authors： Almeida ML,Viana DCF,da Costa VCM,Dos Santos FA,Pereira MC,Pitta MGR,de Melo Rêgo MJB,Pitta IR,Pitta MGR

    更新日期：2020-01-01 00:00:00

  • Osteopontin is a prognostic factor in patients with advanced gastric cancer.

    abstract：OBJECTIVE:Osteopontin (OPN), a phosphorylated sialoprotein, has been shown to overexpress in a variety of cancers and to contribute tumor progression and metastasis by increasing cell migration and adhesion. In the current study, we aimed to investigate the prognostic and predictive role of OPN in patients with advance...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/1386207323666200902135349

    authors： Yazici O,Dogan M,Ozal G,Aktas SH,Demirkazik A,Utkan G,Senler FC,Icli F,Akbulut H

    更新日期：2020-09-02 00:00:00

  • Synthesis of 1,4,5-trisubstituted 1,2,3-triazoles amicable for automation.

    abstract：:A three-component 3+2 cycloaddition reaction followed by Suzuki coupling reaction was carried out to synthesize a library of compounds using automation (parallel synthesizer). Scaffolds that are unexplored in literature were used for the synthesis of library. The iodo-triazoles formed by 3+2 cycloaddition reaction wer...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/1386207311316080009

    authors： Kavitha M,Mahipal B,Mainkar PS,Chandrasekhar S

    更新日期：2013-09-01 00:00:00

  • Investigation of electrochemical behavior of lipid lowering agent atorvastatin calcium in aqueous media and its determination from pharmaceutical dosage forms and biological fluids using boron-doped diamond and glassy carbon electrodes.

    abstract：:The electrochemical behavior of atorvastatin calcium at glassy carbon and boron-doped diamond electrodes has been studied using voltammetric techniques. The possible mechanism of oxidation was discussed with model compounds. The dependence of the peak current and potentials on pH, concentration, scan rate and nature o...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/138620707782152407

    authors： Dogan-Topal B,Uslu B,Ozkan SA

    更新日期：2007-08-01 00:00:00

  • High throughput electrophysiology with Xenopus oocytes.

    abstract：:Voltage-clamp techniques are typically used to study the plasma membrane proteins, such as ion channels and transporters that control bioelectrical signals. Many of these proteins have been cloned and can now be studied as potential targets for drug development. The two approaches most commonly used for heterologous e...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章,评审

    doi：10.2174/138620709787047975

    authors： Papke RL,Smith-Maxwell C

    更新日期：2009-01-01 00:00:00

  • Potential of MurA Enzyme and GBAP in Fsr Quorum Sensing System as Antibacterial Drugs Target: In vitro and In silico Study of Antibacterial Compounds from Myrmecodia pendans.

    abstract：BACKGROUND:Increasing the resistance issue has become the reason for the development of new antibacterial in crucial condition. Many ways are tracked to determine the most effective antibacterial agent. Some proteins that are a key role in bacteria metabolism are targeted, including MurA in cell wall biosynthesis and g...

    journal_title：Combinatorial chemistry & high throughput screening

    pub_type： 杂志文章

    doi：10.2174/1386207323666200628111348

    authors： Apriyanti E,Satari MH,Kurnia D

    更新日期：2021-01-01 00:00:00