Abstract：
:Cyclic peptides have been often utilized as metabolically stable, conformationally restricted mimics of different kinds of biologically active peptides, including peptide antibiotics, endogenous opioid peptides, integrin inhibitors, peptide hormones, anticancer peptides, and so on. And in particular, cyclic compounds which can mimic important secondary structure elements such as beta-turns are of outstanding importance. Since greater chemical and structural diversity are primary features to pursue for finding novel leads for pharmacological and biotechnological applications, we explored the potential utility of the retro-inverso modification. We introduced this modification into the sequence of 13-membered cyclotetrapeptides, which can be regarded as easily available, conformationally stable analogs of cyclotetrapeptides composed of all alpha-residues. In this paper we describe the synthesis of a selected mini-library of partially modified retro-inverso cyclic peptides as conformationally homogeneous scaffolds for medicinal chemistry applications. The different compounds have been obtained by simple scramble of the same residues. Finally, we discuss the conformational features of such molecules as turn mimics. The comparison suggests that the retro-inverso modification allows a higher degree of three-dimensional diversity than normal peptides.
journal_name
Comb Chem High Throughput Screenjournal_title
Combinatorial chemistry & high throughput screeningauthors
Gentilucci L,Cardillo G,Tolomelli A,Squassabia F,De Marco R,Chiriano Gdoi
10.2174/138620709789824754subject
Has Abstractpub_date
2009-12-01 00:00:00pages
929-39issue
10eissn
1386-2073issn
1875-5402journal_volume
12pub_type
杂志文章abstract：:Among the chemometric tools used in rational drug design, we find artificial neural network methods (ANNs), a statistical learning algorithm similar to the human brain, to be quite powerful. Some ANN applications use biological and molecular data of the training series that are inserted to ensure the machine learning,...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：10.2174/1386207318666150803141219
更新日期：2015-01-01 00:00:00
abstract：:In this review various technologies and approaches for the utilization of mass spectrometry in high-throughput analyses are discussed. The use of quadrupole-based mass spectrometry in the screening of chemical libraries against enzymatic targets for the identification of inhibitors and/or activators is highlighted. Th...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：10.2174/138620709789104924
更新日期：2009-09-01 00:00:00
abstract：:The evaluation of the relationships between the hormones involved in the urogenital tract cancer, including bladder, kidney, prostate, and testis, could prove important from diagnostic point of view. The determination of the steroid hormone profiles may likely provide a biomarker for discrimination of hormone-related ...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207311316060007
更新日期：2013-07-01 00:00:00
abstract：:The identification of clones expressing high levels of recombinant protein in Pichia pastoris is usually dependant upon SDS-PAGE, Western blotting, or bioactivity-based assays that are labour and time-consuming. We describe a rapid method that images green fluorescence protein (GFP) of individual P. pastoris clones tr...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/138620710791292967
更新日期：2010-06-01 00:00:00
abstract：:As technologies for high throughput and high content screening continue to evolve, new platforms for quantitative cellular imaging will play an increasingly important role in identifying and profiling lead compounds. To gain insight into the effects of a compound on cell morphology or intracellular events, it is neces...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：10.2174/138620709789383204
更新日期：2009-11-01 00:00:00
abstract：:A comparative structure-affinity study of anthraquinone dyes adsorption on cellulose fibre is presented in this paper. We used receptor-dependent 4D-QSAR methods based on grid and neural (SOM) methodology coupled with IVEPLS procedure. The applied RD 4D-QSAR approach focuses mainly on the ability of mapping dye proper...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207317666140205195252
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abstract：:It is practically impossible in a short period of time to synthesize and test all compounds in any large exhaustive chemical library. We discuss rational approaches to selecting representative subsets of virtual libraries that help direct experimental synthetic efforts for both targeted and diverse library design. For...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207024607400
更新日期：2002-03-01 00:00:00
abstract：:Embryonic stem cells, due to their self-renewal and pluripotency properties, can be used to repair damaged tissues and as an unlimited source of differentiated cells. Although stem cells represent an important opportunity for cell based therapy and small molecules screening (in the context of drug or target discovery)...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/138620712802650540
更新日期：2012-09-01 00:00:00
abstract：:Recyclable ZnO nano-rods catalyst as a most efficient and straightforward protocol for one-pot, three-component synthesis of spirooxindole derivatives at room temperature under solvent-free conditions in both reaction media and work-up procedure is described. This method is of great value because of its environmentall...
journal_title：Combinatorial chemistry & high throughput screening
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doi：10.2174/138620712803901144
更新日期：2012-12-01 00:00:00
abstract：:Formazan-based colorimetric cytotoxicity assays, such as the MTT assay, are typically used to assess cell viability with only metabolically active cells reducing tetrazolium salts into the formazans, which is then quantified by absorbance. Fluorescence offers several advantages compared to colorimetric assays and woul...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207319666160408150913
更新日期：2016-01-01 00:00:00
abstract：:Crucial to the success of directed evolution of enantioselective enzymes for use as catalysts in synthetic organic chemistry is the availability of high-throughput assays for determining the enantiopurity of thousands of samples. Although several such ee-assays are available, they entail time and effort, which means t...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/138620706776843156
更新日期：2006-05-01 00:00:00
abstract：:The transmembrane metzinkin-proteases of the ADAM (a disintegrin and a metalloproteinase)-family ADAM10 and ADAM 17 are both implicated in the ectodomain shedding of various cell surface molecules including the IL6-receptor and the transmembrane chemokines CX3CL1 and CXCL16. These molecules are constitutively released...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：10.2174/1386207053258488
更新日期：2005-03-01 00:00:00
abstract：:A pulsed ultrafiltration-mass spectrometric screening method has been developed to evaluate the metabolic stability of drugs. Pooled human liver microsomes containing cytochrome P450 enzymes were trapped by an ultrafiltration membrane in a stirred flow-through chamber, and eight beta-blocker drugs including acebutolol...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207023330633
更新日期：2002-02-01 00:00:00
abstract：:Two tryptophan-modified permethylated beta-cyclodextrins, 6I-L-Trp-6I-deoxy-2I,3I-di-O-methyl-hexakis(2II-VII,3II-VII,6II-VII-tri-O-methyl-beta-cyclodextrin (3) and 6I-D-Trp- 6I-deoxy-2I,3I-di-O-methyl-hexakis(2II-VII,3II-VII,6II-VII-tri-O-methyl)-beta-cyclodextrin (4), were synthesized, and their binding behaviors we...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/138620707781996457
更新日期：2007-07-01 00:00:00
abstract：:4-Benzothiazol-2-ylamino-4-oxo-2-butenoic acid, prepared from reaction of 2-aminobenzothiazole and maleic anhydride, are used as an acid component in Passerini three-component reaction, under solvent-free media, to produce unsaturated α-benzothiazole acyloxyamides in good yields. ...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/13862073113169990041
更新日期：2013-11-01 00:00:00
abstract：:Clinical application of viral vectors is often hampered by the lack of selectivity of viral particles for the targeted tissue. This drawback decreases the efficiency of gene delivery and raises safety concerns. We successfully established a novel in vitro evolution protocol to engineer adeno-associated virus vectors w...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/138620710792927385
更新日期：2010-11-01 00:00:00
abstract：AIM AND OBJECTIVE:The present method is simple, green and highly efficient for the synthesis of 2-amino-4H-pyran derivatives which are achieved by a one pot three component cyclocondensation of aldehyde, malanonitrile and ethyl acetoacetate or methyl acetoacetate using DABCO under solvent free with grinding conditions ...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207321666180315095422
更新日期：2018-01-01 00:00:00
abstract：:We describe a high-throughput, quantitative technology for fast identification of all different clones present in selectively enriched phage surface-displayed cDNA libraries. The strategy is based on a combination of phage display and high-density arrays. To demonstrate the utility of the method cDNAs of Aspergillus f...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207033329751
更新日期：2003-03-01 00:00:00
abstract：BACKGROUND:The fruit pulp decoction of Crescentia cujete, commonly known as calabash, is traditionally used for the treatment of several respiratory diseases and is available as syrup formulations. Unfortunately, there is no detailed investigation on analytical methods for warranting the quality of these products. AIM...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207323666201207114302
更新日期：2020-12-06 00:00:00
abstract：:Direct renin inhibitors (DRIs) have increasingly shown a significant advantage in the treatment of hypertension and protection of target organs. In this paper, a series of azaindole class renin inhibitors were subjected to 3D-QSAR study using Topomer CoMFA. Five kinds of splitting mode for different fragment cutting a...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207317666140107094708
更新日期：2014-01-01 00:00:00
abstract：:P-glycoprotein (P-gp) is well known to cause multidrug resistance (MDR) in cancer cells. This MDR leads to cancer recurrence which is a major obstacle in cancer treatment. High P-gp expression has been observed in the population of cancer stem cells (CSCs) having self-renewal potential. Early detection and inhibition ...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 历史文章,杂志文章
doi：10.2174/1386207319666160517115158
更新日期：2016-01-01 00:00:00
abstract：:This review describes computational procedures for deriving the amino acid sequences that are compatible with a given protein backbone structure. Such procedures can be used to gain insight into the constraints imposed by the 3D structure of the protein sequence, or to design proteins that are likely to adopt a given ...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：10.2174/1386207013330724
更新日期：2001-12-01 00:00:00
abstract：:The development of stents has been a major advancement over balloon angioplasty, improving vessel revascularization in obstructive coronary artery disease. The development of drug-eluting stents (DES) was the next breakthrough, designed to prevent the development of neointimal hyperplasia (restenosis) following percut...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/138620709788923674
更新日期：2009-08-01 00:00:00
abstract：BACKGROUND:In recent years, Staphylococcus aureus have developed resistance to medicines used for the treatment of human infections. Therefore, the search for antibacterial agents of high potency against Staphylococcus aureus is of great concern. Peptide deformylase (PDF), a metalloprotease catalyzing the removal of a ...
journal_title：Combinatorial chemistry & high throughput screening
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doi：10.2174/1386207321666180220124259
更新日期：2018-01-01 00:00:00
abstract：:Plasmodium falciparum is the most lethal form of the genus Plasmodium which causes malaria, a 'disease of antiquity'. Globally it affects the health and socio-economic development of a large population especially in Sub-Saharan Africa and Southeast Asia. The Plasmodium falciparum dihydrofolate reductase-thymidylate sy...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207317666140521153305
更新日期：2014-01-01 00:00:00
abstract：:Voltage-clamp techniques are typically used to study the plasma membrane proteins, such as ion channels and transporters that control bioelectrical signals. Many of these proteins have been cloned and can now be studied as potential targets for drug development. The two approaches most commonly used for heterologous e...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：10.2174/138620709787047975
更新日期：2009-01-01 00:00:00
abstract：:G protein-coupled receptors (GPCRs) are signaling molecules with a wide variety of skills. Members of this large family of membrane protein have been shown to regulate the activities of the different signaling pathways of the ligand specific manner. α2-adrenoceptors (α2-ARs) are one of the GPCRs and the stimulation of...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207311316070003
更新日期：2013-06-28 00:00:00
abstract：:Polymer-bound triphenylphosphine can replace triphenylphosphine in the Mitsunobu reaction to generate stereochemically inverted secondary alcohols. This method is comparable with the standard Mitsunobu reaction in terms of inversion of stereochemistry, yield, and reaction time, even for sterically very hindered second...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：10.2174/1386207003331706
更新日期：2000-04-01 00:00:00
abstract：:A pulsed ultrafiltration-mass spectrometric screening assay has been developed to generate and identify electrophilic metabolites of xenobiotic compounds formed by hepatic cytochrome P450 enzymes. This assay would be suitable for the early identification of potentially toxic compounds during the initial phase of drug ...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章
doi：
更新日期：1999-06-01 00:00:00
abstract：:Tumorigenesis may be affected by various cellular components in the tumor cells and/or by tumor microenvironmental factors. Cytokines, including chemokines (chemotactic cytokines) are polypeptides or small soluble proteins generated by leukocytes and non-leukocytes, including cancer cells and stromal cells, for exampl...
journal_title：Combinatorial chemistry & high throughput screening
pub_type： 杂志文章,评审
doi：10.2174/138620712799218608
更新日期：2012-03-01 00:00:00
